##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2275599
Sequences flagged as poor quality	0
Sequence length	59
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.63178442247514	31.0	31.0	33.0	28.0	34.0
2	31.4050735652459	31.0	31.0	34.0	30.0	34.0
3	31.66720059201995	31.0	31.0	34.0	30.0	34.0
4	35.50399301458649	37.0	35.0	37.0	33.0	37.0
5	35.22184180956311	37.0	35.0	37.0	33.0	37.0
6	35.32533763637618	37.0	35.0	37.0	33.0	37.0
7	35.247675007767185	37.0	35.0	37.0	33.0	37.0
8	35.30664541511927	37.0	35.0	37.0	33.0	37.0
9	36.93332217143706	39.0	37.0	39.0	33.0	39.0
10	36.99253031839089	39.0	37.0	39.0	33.0	39.0
11	36.72352949706868	37.0	37.0	39.0	34.0	39.0
12	36.658814228693196	39.0	37.0	39.0	32.0	39.0
13	36.69364813396385	39.0	37.0	39.0	32.0	39.0
14	36.84765329919727	39.0	36.0	40.0	31.0	40.0
15	36.27511393703372	38.0	36.0	40.0	30.0	40.0
16	35.53335934846166	38.0	34.0	40.0	28.0	40.0
17	35.55133879035805	38.0	34.0	40.0	27.0	40.0
18	35.74232279061469	38.0	34.0	40.0	27.0	40.0
19	35.91495118428159	38.0	35.0	40.0	29.0	40.0
20	35.84271965315506	38.0	34.0	40.0	29.0	40.0
21	35.81966418512225	38.0	34.0	40.0	29.0	40.0
22	35.82516075987026	38.0	34.0	40.0	29.0	40.0
23	35.7779498936324	38.0	34.0	40.0	29.0	40.0
24	35.77673570782902	38.0	34.0	40.0	29.0	40.0
25	35.78741113878148	38.0	34.0	40.0	29.0	40.0
26	35.36330434316415	38.0	34.0	40.0	27.0	40.0
27	35.03028609170597	37.0	34.0	40.0	26.0	40.0
28	34.819405352173206	37.0	33.0	40.0	26.0	40.0
29	34.722048568311024	37.0	33.0	40.0	26.0	40.0
30	34.55143019486298	37.0	33.0	40.0	25.0	40.0
31	34.52260130189897	37.0	33.0	40.0	25.0	40.0
32	34.43567254160333	36.0	33.0	40.0	25.0	40.0
33	34.19668491680652	36.0	33.0	40.0	24.0	40.0
34	34.035954928790176	36.0	33.0	40.0	23.0	40.0
35	33.88274911353011	36.0	33.0	39.0	23.0	40.0
36	33.51796735716618	35.0	32.0	39.0	22.0	40.0
37	33.40942846257183	35.0	32.0	39.0	22.0	40.0
38	33.17930707475263	35.0	32.0	39.0	21.0	40.0
39	32.77160738776911	35.0	31.0	39.0	20.0	40.0
40	32.444029462132825	35.0	31.0	39.0	18.0	40.0
41	32.08530105699642	35.0	31.0	38.0	17.0	40.0
42	31.829250672020862	35.0	31.0	38.0	15.0	40.0
43	31.494261510925256	35.0	30.0	38.0	13.0	40.0
44	31.193581558086464	35.0	29.0	38.0	11.0	40.0
45	31.03161760925365	35.0	29.0	38.0	10.0	40.0
46	30.70328339922807	35.0	28.0	37.0	10.0	40.0
47	30.302054096525794	34.0	27.0	37.0	10.0	40.0
48	30.004063984911227	34.0	26.0	37.0	9.0	40.0
49	29.696406968011498	34.0	26.0	36.0	9.0	39.0
50	29.286211674376723	34.0	25.0	36.0	9.0	39.0
51	28.998193882138285	33.0	24.0	36.0	9.0	39.0
52	28.422873274245593	33.0	23.0	35.0	8.0	39.0
53	28.29126572827638	33.0	23.0	35.0	8.0	38.0
54	28.10868698747011	33.0	23.0	35.0	8.0	38.0
55	27.885385342496637	33.0	22.0	35.0	8.0	38.0
56	27.56304296143565	33.0	20.0	35.0	8.0	38.0
57	27.12688087839729	33.0	20.0	35.0	8.0	37.0
58	26.846971720412956	33.0	18.0	35.0	8.0	37.0
59	26.10683297013226	31.0	15.0	35.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	12.0
14	37.0
15	183.0
16	643.0
17	1884.0
18	4133.0
19	7675.0
20	12849.0
21	19048.0
22	26914.0
23	35991.0
24	47265.0
25	60326.0
26	74870.0
27	86640.0
28	90442.0
29	88851.0
30	94581.0
31	109041.0
32	130120.0
33	158285.0
34	205750.0
35	246329.0
36	265073.0
37	284878.0
38	210222.0
39	13552.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.231072785670936	22.966744140773486	34.958180241773704	12.844002831781873
2	23.920075549338875	23.856356062733372	33.662653217899994	18.56091517002776
3	17.42657647502921	24.627889184342234	37.21358640076745	20.731947939861108
4	20.090842015662687	23.735816371865166	34.90083270382875	21.272508908643395
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	0.0	100.0
11	98.41206644931731	1.0633244257885506	0.25281255616653026	0.27179656872761854
12	97.08054890162985	2.517798610387858	0.20117779977931088	0.20047468820297426
13	92.92744459810362	5.301329452157432	1.0596770344863045	0.7115489152526433
14	48.148421580427836	34.86778645974093	6.817589566527318	10.166202393303916
15	34.35772295558224	32.60618412998072	16.671961975725953	16.36413093871108
16	35.80195807785115	26.17750315411459	21.45733936427288	16.563199403761384
17	23.66484604712869	25.241617701537045	25.153816643442013	25.939719607892254
18	22.442969960876237	31.63865865646803	27.228039738108517	18.690331644547218
19	27.253483588277195	29.84568019233617	26.06236863348947	16.838467585897163
20	23.460240578414737	32.61413807968803	25.844535878245683	18.081085463651547
21	26.235290136794752	30.78358709069568	20.35424518994779	22.62687758256178
22	26.74363980648612	32.114137859965666	22.22966348640512	18.912558847143103
23	22.1341721454439	35.325204484621416	21.331921836843836	21.20870153309085
24	22.52532190425466	37.06597691421028	21.789999028827133	18.618702152707925
25	23.447584570040682	34.181505616762884	22.131799143873767	20.23911066932267
26	21.943541019309638	33.19235946227784	19.23849500724864	25.625604511163875
27	24.265435166740712	36.76921988452271	17.582008077873123	21.383336870863452
28	25.12876829353502	33.90803915804146	17.38997072858619	23.573221819837325
29	25.56030302351161	32.827796109947315	15.056343406724999	26.555557459816075
30	27.403290298510413	34.610623400695815	17.7223227818258	20.263763518967973
31	22.29202069433147	38.309869181696776	15.211203731413136	24.18690639255862
32	21.749833780028908	42.34247773882833	16.777516601123484	19.130171880019283
33	25.726896522629865	39.43708887198491	17.234275458901152	17.601739146484068
34	20.867209029358865	42.98551721986168	16.659833301034148	19.487440449745318
35	25.14937825161639	40.667314408206366	17.574185961586377	16.609121378590867
36	23.504712385618028	39.40149384843287	20.389708380079266	16.70408538586983
37	22.76380856205333	40.98494506281643	18.318781120926843	17.932465254203397
38	20.6111885266253	42.0729662827238	21.634611370456746	15.681233820194155
39	20.87766781405687	43.53930547517379	19.351432304197708	16.23159440657163
40	21.656319940376136	43.95225169285098	18.20689849134228	16.184529875430602
41	23.605433118928246	42.38277482104712	16.642079733731645	17.369712326292987
42	21.66524066850091	42.64042126930096	15.819395244944298	19.874942817253828
43	24.647048974797407	39.427860532545495	18.319220565662054	17.605869926995048
44	21.34435812285029	41.96815871337613	16.172401200738797	20.51508196303479
45	21.08521756249673	37.424080428933216	17.946219874415483	23.544482134154567
46	21.644806488313627	37.13250884712113	16.019210766044456	25.203473898520784
47	23.611277733906544	38.06487874181699	17.57031884791653	20.753524676359937
48	25.93180960265846	36.167136652810974	17.772595259533862	20.1284584849967
49	23.50888711060253	39.089883586695194	19.03824004141327	18.362989261289005
50	22.25264644605662	40.67988252763338	16.053970844599597	21.0135001817104
51	20.12735987315867	41.00502768721554	19.938618359385814	18.92899408023997
52	22.4863431562415	38.190647825034205	20.177192906131527	19.145816112592772
53	20.178071795601948	39.09379464483857	18.97684961190438	21.7512839476551
54	21.415284503113245	38.11005366059661	20.717402319125647	19.757259517164492
55	20.6425209362458	37.69807422133689	20.71252448256481	20.946880359852504
56	20.476938160018527	37.86167949625571	21.461074644522167	20.20030769920359
57	20.99113244468819	35.99918087501357	22.46577714263365	20.54390953766459
58	21.60991457633792	34.87064285051979	21.91348299942125	21.60595957372103
59	21.625602753384936	34.60811856570511	21.344489956270856	22.421788724639093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	5.5
5	11.0
6	561.5
7	1112.0
8	1589.0
9	3356.5
10	4647.0
11	5494.5
12	6342.0
13	8134.5
14	10246.0
15	10565.0
16	10211.0
17	9857.0
18	9020.0
19	8366.0
20	8549.0
21	10142.5
22	11736.0
23	14829.0
24	17922.0
25	23929.0
26	37826.5
27	45717.0
28	54011.5
29	62306.0
30	68010.5
31	78038.5
32	82362.0
33	83282.0
34	84202.0
35	80965.0
36	74866.5
37	72005.0
38	70979.0
39	69953.0
40	70005.5
41	70058.0
42	77250.5
43	88781.5
44	93120.0
45	86941.0
46	80762.0
47	84424.0
48	94220.5
49	100355.0
50	96829.5
51	93304.0
52	99516.5
53	124629.0
54	143529.0
55	148513.0
56	153497.0
57	144176.5
58	127769.5
59	120683.0
60	108902.0
61	97121.0
62	81369.5
63	65618.0
64	59052.0
65	44742.5
66	36999.0
67	29710.0
68	22421.0
69	19076.5
70	13612.0
71	11492.0
72	10458.5
73	9425.0
74	6458.5
75	2951.5
76	2411.0
77	1532.0
78	653.0
79	474.5
80	296.0
81	233.5
82	91.0
83	11.0
84	8.5
85	6.0
86	3.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	2275599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.06600788538997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.84590270425097	30.606912299929938
2	4.3906561678100955	2.991442552693097
3	1.4467978787190763	1.4785988383502862
4	0.8339359701832801	1.1363547734469581
5	0.5035488073184297	0.857694882039417
6	0.35947851612627874	0.7347598778991659
7	0.26531902523101275	0.6326852003964563
8	0.23305466266565622	0.6351393580876117
9	0.17730962860487182	0.5436208085588219
>10	1.531395521349746	10.774179408396659
>50	0.1257370089098984	2.8612307841531495
>100	0.18511320416835383	16.708196916046447
>500	0.07736882710605406	18.541916374258882
>1k	0.02438207755630929	11.497267925743124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGTTCTACCGGGACATGATCTCACAAAATATCATTTCAAAACCAATTATTACACAGTT	3170	0.1393039810616897	No Hit
AGACTCTACCGGGATACTACCTTCTGAAGAACCCTGCTTTCTTCTGAAAAATCTGAGGA	3161	0.13890848080000034	No Hit
TAACTCTACCGGGGGAGAGATAGCTCAGAAGTTAAGAGCACTGACTGTTCTTCATAAGG	2866	0.12594486111129422	No Hit
TCTTTCTACCGGGGAGCATCCACAGCGCTCACTACCCTGGCCTGGCCCCTGTGAAAGAG	2765	0.1215064692856694	No Hit
TTCTTCTACCGGGCAGGAGGCTGAGAATCACTTAAACCCGGGAGACAGAGGTCGCAGTT	2604	0.11443140904878232	No Hit
AAAATCTACCGGGTGGCTCAGGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGTA	2565	0.11271757458146184	No Hit
TACTTCTACCGGGGGACCATCTTCGACAGTGACCTTATAGACAGCACCATGCTGGGGGG	2411	0.10595012565922203	No Hit
GTTATCTACCGGGGCAGAGTTCTATCTACGGGGGAGAGATGGCTCAGTGGCTAAAAGCA	2383	0.10471968040063298	No Hit
TATGTCTACCGGGGGAGAGATGGCTCAGTGGCTAAAAGCACTGACTGCTCTTCCAGAGG	2329	0.10234667883049695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.015380565732363216	0.0	0.0	0.0	0.0
12	0.03919847038076568	0.0	0.0	0.0	0.0
13	0.05018458876102512	0.0	0.0	0.0	0.0
14	0.09245917228826345	0.0	0.0	0.0	0.0
15	0.1608367730869982	0.0	0.0	0.0	0.0
16	0.23932160279557163	0.0	0.0	0.0	0.0
17	0.3031729228216395	0.0	0.0	0.0	0.0
18	0.39110581433723607	0.0	0.0	0.0	0.0
19	0.4791265947998747	0.0	0.0	0.0	0.0
20	0.5682020426270182	0.0	0.0	0.0	0.0
21	0.6571896015071197	0.0	0.0	0.0	0.0
22	0.7468363274900367	0.0	0.0	0.0	0.0
23	0.8289246040273353	0.0	0.0	0.0	0.0
24	0.918351607642647	0.0	0.0	0.0	0.0
25	1.012568558871752	0.0	0.0	0.0	0.0
26	1.1179913508487216	0.0	0.0	0.0	0.0
27	1.2164709160093672	0.0	0.0	0.0	0.0
28	1.3168400935314175	0.0	0.0	0.0	0.0
29	1.4360175057204718	0.0	0.0	0.0	0.0
30	1.5715862065328734	0.0	0.0	0.0	0.0
31	1.6984099571145883	0.0	0.0	0.0	0.0
32	1.7967576888546708	0.0	0.0	0.0	0.0
33	1.878538354077322	0.0	0.0	0.0	0.0
34	1.9584733514120898	0.0	0.0	0.0	0.0
35	2.0292678982544814	0.0	0.0	0.0	0.0
36	2.0979531103678637	0.0	0.0	0.0	0.0
37	2.1711646032539127	0.0	0.0	0.0	0.0
38	2.249649432962486	0.0	0.0	0.0	0.0
39	2.3356048231696356	0.0	0.0	0.0	0.0
40	2.422043602585517	0.0	0.0	0.0	0.0
41	2.503296934125916	0.0	0.0	0.0	0.0
42	2.5870990451305347	0.0	0.0	0.0	0.0
43	2.673362046652332	0.0	0.0	0.0	0.0
44	2.762173827638349	0.0	0.0	0.0	0.0
45	2.845580438381279	0.0	0.0	0.0	0.0
46	2.928108159653788	0.0	0.0	0.0	0.0
47	3.006461155941798	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	215	0.0	53.000004	18
AACGTTA	50	3.6379788E-12	53.0	29
CTTAACA	15	0.006697106	53.0	52
TTAGGTG	20	3.1388993E-4	53.0	36
TACTTTA	35	3.34403E-8	53.0	48
ATTAGGC	15	0.006697106	53.0	41
ATAGGTA	35	3.34403E-8	53.0	52
GATAACG	50	3.6379788E-12	53.0	18
CAACGTC	80	0.0	53.0	23
CGACGAA	15	0.006697106	53.0	32
TCCGATA	70	0.0	53.0	25
ACGTTCC	55	0.0	53.0	43
ACGTTAT	50	3.6379788E-12	53.0	30
ACGTTAG	45	7.6397555E-11	53.0	36
CGTGCGA	55	0.0	53.0	49
TTAGCCA	190	0.0	53.0	31
CGTGCAC	25	1.4791944E-5	53.0	47
TCCGAAC	105	0.0	53.0	30
CGCATTA	15	0.006697106	53.0	21
AACTTAT	210	0.0	53.0	38
>>END_MODULE