FastQCFastQC Report
Fri 17 Jun 2016
SRR1528122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340163
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACGCCGGGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAAGC21050.6188209770022018No Hit
TTCAAACGCCGAGAGACCCAACCTTCGGGAGGCATCACGCTTTAGACTACCA20390.5994185140653158No Hit
ACCGAACGCCGGGTAACACCGGGGTCTTATTCTTAGTCATTCAGGAAGTTTT16740.492117014490112No Hit
CTGCAACGCCGGAGACAGAAAAAGGCCAGGCGCGGTGGCTCATGCCCATAAT16290.4788880624876897No Hit
GTTGAACGCCGGGGGGCCAACATAGTGAAACCCCATCTCTACTAAAAAATAC14010.41186137234208303No Hit
GGACAACGCCGGGAGAGTGGGTAACGCCGGGAAAACCCCCAACACTCCGATG13530.3977504902061659No Hit
AGTTAACGCCGGGAGAGTTTGAAACGCCGGGGCATTTTCGCTATTGTTCAAT12300.36159135473287807No Hit
AGTTAACGCCCGGGCAGCACTTTGGGAGGCCGAGGCAGGAGGATCACCTGAG11240.33042982334939425No Hit
ATAGAACGCCGGGGGTACACAATTGCCTATAATGAGCAAAATTGAATTAGGA10490.3083815700120236No Hit
CGTAAACGCCGGGTGGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCACCT10050.2954465947207662No Hit
CCACAACGCCGGTATCCACCAGGGACTAGGACTAGGAGGCTATAGTTCTGTC9690.28486343311882834No Hit
CTAGAACGCCGGGCAGGGATGCTGGGATGGTTTAACATATGCAAGTCAATAA9350.2748682249392203No Hit
AATAAACGCCGGGACGGGAGACATCTCATATGTACCCCGAATTGAGTAAATT9310.2736923180945605No Hit
CGCAAACGCCGGGAGAGTTAAAAACGCCGGGGGGAATTCTCTTGCTTCAACA9110.26781278387126173No Hit
TCACAACGCCGGGAGAGTTGTCAACGCGGGGCGGGCGCCTGTAGTGCCAGCT8690.2554657620023342No Hit
AAACAACGCCGGTCTGTTTCTGATTCACATTGCTAAAGAACCACAGTGGATA8430.24782236751204567No Hit
GTTAAACGCCGGGTGCTCCCCAAGTTAAGAAAATGAGATGTTTTATATAAGC8330.24488260040039628No Hit
ACATAACGCCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC8010.23547534564311817No Hit
GGCCAACGCCGGGGTATCAACGGGGCACAAGCCTTGTCCTGTCCTGGGTTCT7560.22224639364069576No Hit
TACCAACGCCGGGCGTTTTTGATGTAAATATTAAGAACTTGGTCTATTTCTG7450.21901264981788143No Hit
CACAAACGCCGGGGAAACCTAGAAGAAAGAGATAAGTTCCTAGACACTTCTA7120.20931141834943834No Hit
GTACAACGCCGGGAGAATAGTCAAACTCATAGAAACGGAAAGTACAATGGTG7080.2081355115047786No Hit
CTAGAACGCCGGGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATA6780.19931621016983037No Hit
GCTTAACGCCGGGGGAAGAGCTAGCATCTCTGACGAAAAACAGCAGACGGAA6570.1931426992353666No Hit
GTATAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT6500.191084862257212No Hit
GAATAACGCCCGGCATATATATGTATCTTTTTGTTTTTTTGAGATGTAGTCC6490.19079088554604706No Hit
ATTCAACGCCGGGATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT6000.17638602669896492No Hit
CACAAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT5970.17550409656547009No Hit
CTACAACGCCGGGAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCG5730.16844865549751148No Hit
TTCCAACGCCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC5640.16580286509702702No Hit
AGCCAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT5160.1516919829611098No Hit
CTCTAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT5060.14875221584946038No Hit
GAATAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC5030.14787028571596558No Hit
AACCAACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4800.1411088213591719No Hit
ACACAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT4760.13993291451451215No Hit
TGCTAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC4730.13905098438101732No Hit
TTTCAACGCCGGGCTGCTGGCCGTGGGCGGACTCGGCTTCGTGATGACACTG4520.13287747344655357No Hit
GAATAACGCCGGGACTCCAGCCTGGGTAACAGAGCAAGACTCTGTCTCAAAA4240.1246461255339352No Hit
TTTCAACGCCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4210.12376419540044037No Hit
TCGAAACGCCGGGAAGCAAACACACCAAAAACAAACCAATACTAAACTCCCT4180.12288226526694554No Hit
CCTGAACGCCAGGGTTAGAACTTATTTCCAAGTCAAAAATTAGTATCTCCAT4110.12082442828879095No Hit
AGGCAACGCCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4040.11876659131063638No Hit
AGGCAACGCCGGGATACTGTTGGTCTTTACAATTCACCTCATCTTATAGATT4030.11847261459947142No Hit
CACCAACGCCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG3810.11200512695384271No Hit
GTTAAACGCCGGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA3490.10259787219656458No Hit
GCACAACGCCGGGTATGACCTAAAGTAGCACTGTTTCAGCCAACCCATGAGA3480.10230389548539964No Hit
GGCAAACGCCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.10142196535190481No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA700.046.00000442
GAGAATA700.046.00000413
TTTTGAG700.046.00000437
GGGTCTC351.0167241E-746.00000411
GCCTTGT1450.046.00000431
GTCATAC700.046.00000443
CACACCG700.046.00000421
ATAAGTT1400.046.00000432
TTACTTC700.046.00000428
GAAACCT1450.046.00000414
CTGGGTT1450.046.00000444
TCCTAGA1400.046.00000438
TAAGTTC1400.046.00000433
TTCCGCC700.046.00000438
CACGCCA700.046.00000431
CACAAGC1450.046.00000426
CCGCCCA700.046.00000440
AGGGTTA700.046.00000411
GATGTAG700.046.00000443
ACACTTC1400.046.00000444
Produced by FastQC (version 0.11.5)