##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528122_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340163
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.050790356387967	31.0	31.0	34.0	28.0	34.0
2	31.72382945823032	31.0	31.0	34.0	30.0	34.0
3	31.883785126542275	31.0	31.0	34.0	30.0	34.0
4	35.60057090277308	37.0	35.0	37.0	33.0	37.0
5	35.57924877191229	37.0	35.0	37.0	33.0	37.0
6	35.85027472123658	37.0	35.0	37.0	35.0	37.0
7	35.46252531874425	37.0	35.0	37.0	33.0	37.0
8	35.18048112228549	37.0	35.0	37.0	32.0	37.0
9	36.36814409562474	38.0	35.0	39.0	31.0	39.0
10	37.156510261257104	39.0	37.0	39.0	32.0	39.0
11	37.60214955771204	39.0	37.0	39.0	35.0	39.0
12	37.50854443311001	39.0	37.0	39.0	35.0	39.0
13	37.337546999526694	39.0	37.0	39.0	35.0	39.0
14	37.24894829831581	40.0	37.0	40.0	31.0	40.0
15	36.41492460967242	39.0	35.0	40.0	30.0	40.0
16	36.157671469266205	38.0	35.0	40.0	30.0	40.0
17	36.12355547193551	38.0	35.0	40.0	30.0	40.0
18	36.09382266736829	38.0	35.0	40.0	30.0	40.0
19	36.369249448058724	38.0	35.0	40.0	30.0	40.0
20	36.5819768757919	39.0	35.0	40.0	30.0	40.0
21	36.61963823225924	39.0	35.0	40.0	30.0	40.0
22	36.668700005585556	39.0	35.0	40.0	30.0	40.0
23	36.64748076657367	39.0	35.0	40.0	31.0	40.0
24	36.49143498852021	39.0	35.0	40.0	30.0	40.0
25	36.35671428109465	38.0	35.0	40.0	30.0	40.0
26	35.892733777630134	38.0	35.0	40.0	30.0	40.0
27	35.59934501988752	38.0	35.0	40.0	29.0	40.0
28	35.4417940810729	38.0	34.0	40.0	28.0	40.0
29	35.4105531759774	37.0	34.0	40.0	28.0	40.0
30	35.15904728027446	37.0	34.0	40.0	27.0	40.0
31	34.97726090139139	37.0	33.0	40.0	26.0	40.0
32	34.757195814947536	36.0	33.0	40.0	26.0	40.0
33	34.56358275297431	36.0	33.0	40.0	26.0	40.0
34	34.25706793507818	35.0	33.0	39.0	25.0	40.0
35	33.96522255506919	35.0	33.0	39.0	24.0	40.0
36	33.60857882838521	35.0	33.0	38.0	24.0	40.0
37	33.21420907035745	35.0	33.0	37.0	23.0	40.0
38	32.71874954066139	35.0	33.0	37.0	21.0	40.0
39	32.156442646613534	35.0	32.0	36.0	20.0	40.0
40	31.726425272589907	35.0	31.0	36.0	18.0	40.0
41	31.179437504960855	35.0	31.0	35.0	15.0	39.0
42	30.60167037567284	35.0	30.0	35.0	12.0	39.0
43	30.038519768463942	35.0	28.0	35.0	10.0	39.0
44	29.511998659466197	34.0	26.0	35.0	9.0	39.0
45	29.141726172452618	34.0	24.0	35.0	9.0	38.0
46	28.64927402451178	34.0	23.0	35.0	8.0	37.0
47	28.13153693964364	33.0	23.0	35.0	8.0	37.0
48	27.71461916786952	33.0	20.0	35.0	8.0	36.0
49	27.343267786325967	33.0	20.0	35.0	8.0	36.0
50	26.925218204213863	33.0	20.0	35.0	8.0	35.0
51	26.464938867542912	33.0	17.0	35.0	8.0	35.0
52	25.694937427057027	33.0	12.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	5.0
15	11.0
16	26.0
17	119.0
18	257.0
19	572.0
20	1082.0
21	1771.0
22	2407.0
23	3518.0
24	4655.0
25	6307.0
26	8260.0
27	10414.0
28	12966.0
29	15796.0
30	17501.0
31	17932.0
32	18128.0
33	20577.0
34	25686.0
35	36318.0
36	50298.0
37	58779.0
38	24567.0
39	2209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.123608387743523	25.265240487648565	37.55758268829943	6.053568436308476
2	28.05831322042668	22.012388178608493	32.20103303416303	17.7282655668018
3	17.84497432113428	25.420460191143658	32.738422462172544	23.996143025549515
4	17.402833347542206	26.32590846153168	30.288420551323924	25.98283763960219
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	0.0	100.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	0.0	100.0
11	97.21486463842335	1.6597925112372596	0.20989937177176826	0.9154434785676279
12	95.98104438166408	3.4748047259696087	0.2757501550727151	0.2684007372935916
13	89.96980859176335	6.956076939584846	2.1166323203875788	0.9574821482642146
14	39.57132315977928	38.00119354544733	14.311374252931683	8.116109041841705
15	35.62174604527829	35.63879669452586	20.494292442152734	8.245164818043115
16	32.4570867495877	37.088983810702516	18.123664243318643	12.330265196391142
17	27.403039131240025	31.04011900177268	19.924565575915075	21.632276291072223
18	16.099928563659187	38.70761958237669	28.127397747550436	17.06505410641369
19	19.73642048076951	42.840932141355765	25.889059068740572	11.533588309134151
20	20.975238341618578	40.01023038954854	23.035427133462488	15.979104135370395
21	21.43237212748006	40.55555718875951	15.35058192689975	22.66148875686068
22	21.857462451824567	43.41359877470507	17.019487716183125	17.709451057287243
23	17.644776180830952	47.15063072703381	17.550409656547007	17.654183435588234
24	16.76754967471477	45.87918145124543	14.598883476450997	22.754385397588802
25	25.061220650100097	37.17423705694035	13.84718502600224	23.91735726695731
26	24.75901259102254	37.74425789988917	12.750357916645843	24.746371592442447
27	18.55287024161946	45.63694464124552	11.881950711864606	23.928234405270416
28	17.220861763331108	46.41333713543213	11.58944388425549	24.77635721698127
29	26.168924897769596	47.31172996475219	11.363081816658484	15.156263320819724
30	26.258587794674902	51.74019514174088	10.843624968030033	11.15759209555419
31	20.5733721774561	54.89103753200671	10.574930254025276	13.960660036511907
32	17.523363799119835	57.84079985183573	12.170929818939744	12.464906530104686
33	13.989175777494905	61.066018350026305	13.402398262009685	11.5424076104691
34	14.441311959266587	62.744037417355806	11.727318961791847	11.08733166158577
35	16.751086978889532	61.16832224551171	12.182982864097507	9.89760791150125
36	15.188306782336703	59.48030797000262	13.034927373053506	12.296457874607174
37	13.079905809861742	60.669737743375975	12.083030782301426	14.167325664460861
38	13.87158509302893	58.05011127018518	13.501468413672269	14.576835223113624
39	14.382810593744763	58.53046921622869	13.219250770953925	13.86746941907262
40	15.103053536098871	57.818751598498366	13.207785679218492	13.87040918618427
41	16.362155790018313	59.056099575791606	11.845791576391317	12.73595305779876
42	14.978407410564934	56.783659598486615	14.095301370225451	14.142631620723007
43	17.20969064830684	54.65820797676408	14.62122570649953	13.510875668429549
44	19.580612823852096	55.39403168480993	12.426983534364409	12.598371956973569
45	18.165114959592902	53.097191640478236	12.213850418769825	16.52384298115903
46	18.258599553743352	55.81383042835346	11.722027380990879	14.205542636912305
47	17.758545168051786	54.88368811422759	12.910869200941901	14.44689751677872
48	18.819801095357224	54.52444857318404	13.395930774364054	13.259819557094687
49	18.74983463809997	57.426292689093174	12.762998915225937	11.060873757580925
50	17.527773449787308	57.0864556109865	12.34378812510473	13.041982814121466
51	17.37372965313688	54.705832203972804	14.277566931147714	13.642871211742605
52	19.234896211522123	52.673865176400724	14.26904160652393	13.82219700555322
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	17.5
10	33.0
11	333.0
12	633.0
13	685.0
14	1591.5
15	2446.0
16	2694.5
17	2943.0
18	2875.5
19	2808.0
20	3105.5
21	3403.0
22	3398.0
23	3393.0
24	4296.5
25	5200.0
26	7399.5
27	9599.0
28	13197.5
29	16796.0
30	19937.0
31	23078.0
32	23687.0
33	24296.0
34	23498.0
35	22700.0
36	20356.5
37	18013.0
38	17367.5
39	15094.5
40	13467.0
41	12509.5
42	11552.0
43	12116.5
44	12681.0
45	12330.0
46	11979.0
47	15329.5
48	18680.0
49	17466.0
50	16252.0
51	16314.5
52	16377.0
53	18533.0
54	20689.0
55	19425.0
56	18161.0
57	15848.5
58	13536.0
59	12498.0
60	11460.0
61	8966.5
62	6473.0
63	6748.0
64	5343.0
65	3663.0
66	3750.0
67	3837.0
68	2459.0
69	1081.0
70	636.5
71	192.0
72	141.0
73	90.0
74	87.5
75	85.0
76	61.0
77	37.0
78	30.0
79	23.0
80	18.0
81	13.0
82	8.0
83	3.0
84	4.0
85	5.0
86	3.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	340163.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.21963808944283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.6967352605295	38.77917894846294
2	5.36596449710542	4.745620161256015
3	1.610528105262067	2.136509098426941
4	0.7697988903184924	1.3616091332615372
5	0.47864483192347773	1.058275062051919
6	0.3462052621001555	0.9185442836837745
7	0.2669036668024502	0.826166848552471
8	0.25607264658103635	0.9058751805135382
9	0.22801362697040528	0.9074412057683289
>10	2.6458240480030004	25.108232832387788
>50	0.23264989736976205	6.771896857001181
>100	0.08066085266974558	7.308684571997009
>500	0.015332228193422713	4.865578266733187
>1k	0.006666186171053354	4.306387549903376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAACGCCGGGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAAGC	2105	0.6188209770022018	No Hit
TTCAAACGCCGAGAGACCCAACCTTCGGGAGGCATCACGCTTTAGACTACCA	2039	0.5994185140653158	No Hit
ACCGAACGCCGGGTAACACCGGGGTCTTATTCTTAGTCATTCAGGAAGTTTT	1674	0.492117014490112	No Hit
CTGCAACGCCGGAGACAGAAAAAGGCCAGGCGCGGTGGCTCATGCCCATAAT	1629	0.4788880624876897	No Hit
GTTGAACGCCGGGGGGCCAACATAGTGAAACCCCATCTCTACTAAAAAATAC	1401	0.41186137234208303	No Hit
GGACAACGCCGGGAGAGTGGGTAACGCCGGGAAAACCCCCAACACTCCGATG	1353	0.3977504902061659	No Hit
AGTTAACGCCGGGAGAGTTTGAAACGCCGGGGCATTTTCGCTATTGTTCAAT	1230	0.36159135473287807	No Hit
AGTTAACGCCCGGGCAGCACTTTGGGAGGCCGAGGCAGGAGGATCACCTGAG	1124	0.33042982334939425	No Hit
ATAGAACGCCGGGGGTACACAATTGCCTATAATGAGCAAAATTGAATTAGGA	1049	0.3083815700120236	No Hit
CGTAAACGCCGGGTGGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCACCT	1005	0.2954465947207662	No Hit
CCACAACGCCGGTATCCACCAGGGACTAGGACTAGGAGGCTATAGTTCTGTC	969	0.28486343311882834	No Hit
CTAGAACGCCGGGCAGGGATGCTGGGATGGTTTAACATATGCAAGTCAATAA	935	0.2748682249392203	No Hit
AATAAACGCCGGGACGGGAGACATCTCATATGTACCCCGAATTGAGTAAATT	931	0.2736923180945605	No Hit
CGCAAACGCCGGGAGAGTTAAAAACGCCGGGGGGAATTCTCTTGCTTCAACA	911	0.26781278387126173	No Hit
TCACAACGCCGGGAGAGTTGTCAACGCGGGGCGGGCGCCTGTAGTGCCAGCT	869	0.2554657620023342	No Hit
AAACAACGCCGGTCTGTTTCTGATTCACATTGCTAAAGAACCACAGTGGATA	843	0.24782236751204567	No Hit
GTTAAACGCCGGGTGCTCCCCAAGTTAAGAAAATGAGATGTTTTATATAAGC	833	0.24488260040039628	No Hit
ACATAACGCCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC	801	0.23547534564311817	No Hit
GGCCAACGCCGGGGTATCAACGGGGCACAAGCCTTGTCCTGTCCTGGGTTCT	756	0.22224639364069576	No Hit
TACCAACGCCGGGCGTTTTTGATGTAAATATTAAGAACTTGGTCTATTTCTG	745	0.21901264981788143	No Hit
CACAAACGCCGGGGAAACCTAGAAGAAAGAGATAAGTTCCTAGACACTTCTA	712	0.20931141834943834	No Hit
GTACAACGCCGGGAGAATAGTCAAACTCATAGAAACGGAAAGTACAATGGTG	708	0.2081355115047786	No Hit
CTAGAACGCCGGGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATA	678	0.19931621016983037	No Hit
GCTTAACGCCGGGGGAAGAGCTAGCATCTCTGACGAAAAACAGCAGACGGAA	657	0.1931426992353666	No Hit
GTATAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	650	0.191084862257212	No Hit
GAATAACGCCCGGCATATATATGTATCTTTTTGTTTTTTTGAGATGTAGTCC	649	0.19079088554604706	No Hit
ATTCAACGCCGGGATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT	600	0.17638602669896492	No Hit
CACAAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	597	0.17550409656547009	No Hit
CTACAACGCCGGGAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCG	573	0.16844865549751148	No Hit
TTCCAACGCCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC	564	0.16580286509702702	No Hit
AGCCAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	516	0.1516919829611098	No Hit
CTCTAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	506	0.14875221584946038	No Hit
GAATAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC	503	0.14787028571596558	No Hit
AACCAACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	480	0.1411088213591719	No Hit
ACACAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	476	0.13993291451451215	No Hit
TGCTAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC	473	0.13905098438101732	No Hit
TTTCAACGCCGGGCTGCTGGCCGTGGGCGGACTCGGCTTCGTGATGACACTG	452	0.13287747344655357	No Hit
GAATAACGCCGGGACTCCAGCCTGGGTAACAGAGCAAGACTCTGTCTCAAAA	424	0.1246461255339352	No Hit
TTTCAACGCCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC	421	0.12376419540044037	No Hit
TCGAAACGCCGGGAAGCAAACACACCAAAAACAAACCAATACTAAACTCCCT	418	0.12288226526694554	No Hit
CCTGAACGCCAGGGTTAGAACTTATTTCCAAGTCAAAAATTAGTATCTCCAT	411	0.12082442828879095	No Hit
AGGCAACGCCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC	404	0.11876659131063638	No Hit
AGGCAACGCCGGGATACTGTTGGTCTTTACAATTCACCTCATCTTATAGATT	403	0.11847261459947142	No Hit
CACCAACGCCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG	381	0.11200512695384271	No Hit
GTTAAACGCCGGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA	349	0.10259787219656458	No Hit
GCACAACGCCGGGTATGACCTAAAGTAGCACTGTTTCAGCCAACCCATGAGA	348	0.10230389548539964	No Hit
GGCAAACGCCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	345	0.10142196535190481	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.06849657370143138	0.0	0.0	0.0	0.0
12	0.11935454473296625	0.0	0.0	0.0	0.0
13	0.14316665833732653	0.0	0.0	0.0	0.0
14	0.2704585742717462	0.0	0.0	0.0	0.0
15	0.40186616416247506	0.0	0.0	0.0	0.0
16	0.5944209099755118	0.0	0.0	0.0	0.0
17	0.7422911956914773	0.0	0.0	0.0	0.0
18	0.9283784538588853	0.0	0.0	0.0	0.0
19	1.1297525010068703	0.0	0.0	0.0	0.0
20	1.3605242192713494	0.0	0.0	0.0	0.0
21	1.5921778676693232	0.0	0.0	0.0	0.0
22	1.8150122147323489	0.0	0.0	0.0	0.0
23	1.9981597057881075	0.0	0.0	0.0	0.0
24	2.195712055690948	0.0	0.0	0.0	0.0
25	2.432363308178726	0.0	0.0	0.0	0.0
26	2.7069375564067815	0.0	0.0	0.0	0.0
27	2.9597575280086312	0.0	0.0	0.0	0.0
28	3.2305100789915424	0.0	0.0	0.0	0.0
29	3.5618218324744313	0.0	0.0	0.0	0.0
30	3.997495318420875	0.0	0.0	0.0	0.0
31	4.374373462134329	0.0	0.0	0.0	0.0
32	4.631309107692489	0.0	0.0	0.0	0.0
33	4.845618130131731	0.0	0.0	0.0	0.0
34	5.061691012837963	0.0	0.0	0.0	0.0
35	5.258655409318473	0.0	0.0	0.0	0.0
36	5.478843965981015	0.0	0.0	0.0	0.0
37	5.708145800689669	0.0	0.0	0.0	0.0
38	5.938035588820654	0.0	0.0	0.0	0.0
39	6.18291818922105	0.0	0.0	0.0	0.0
40	6.439559858068044	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCATA	70	0.0	46.000004	42
GAGAATA	70	0.0	46.000004	13
TTTTGAG	70	0.0	46.000004	37
GGGTCTC	35	1.0167241E-7	46.000004	11
GCCTTGT	145	0.0	46.000004	31
GTCATAC	70	0.0	46.000004	43
CACACCG	70	0.0	46.000004	21
ATAAGTT	140	0.0	46.000004	32
TTACTTC	70	0.0	46.000004	28
GAAACCT	145	0.0	46.000004	14
CTGGGTT	145	0.0	46.000004	44
TCCTAGA	140	0.0	46.000004	38
TAAGTTC	140	0.0	46.000004	33
TTCCGCC	70	0.0	46.000004	38
CACGCCA	70	0.0	46.000004	31
CACAAGC	145	0.0	46.000004	26
CCGCCCA	70	0.0	46.000004	40
AGGGTTA	70	0.0	46.000004	11
GATGTAG	70	0.0	46.000004	43
ACACTTC	140	0.0	46.000004	44
>>END_MODULE