FastQCFastQC Report
Fri 17 Jun 2016
SRR1528121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338879
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACGCCGGGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAAGC20250.5975584205571901No Hit
TTCAAACGCCGAGAGACCCAACCTTCGGGAGGCATCACGCTTTAGACTACCA19500.57542662720322No Hit
ACCGAACGCCGGGTAACACCGGGGTCTTATTCTTAGTCATTCAGGAAGTTTT18050.5326384933855447No Hit
CTGCAACGCCGGAGACAGAAAAAGGCCAGGCGCGGTGGCTCATGCCCATAAT15310.451783674999041No Hit
GGACAACGCCGGGAGAGTGGGTAACGCCGGGAAAACCCCCAACACTCCGATG13680.4036839107764128No Hit
GTTGAACGCCGGGGGGCCAACATAGTGAAACCCCATCTCTACTAAAAAATAC13330.39335574054456013No Hit
AGTTAACGCCGGGAGAGTTTGAAACGCCGGGGCATTTTCGCTATTGTTCAAT11750.3467314292121967No Hit
AGTTAACGCCCGGGCAGCACTTTGGGAGGCCGAGGCAGGAGGATCACCTGAG11440.33758362129255576No Hit
ATAGAACGCCGGGGGTACACAATTGCCTATAATGAGCAAAATTGAATTAGGA10800.31869782429716803No Hit
CTAGAACGCCGGGCAGGGATGCTGGGATGGTTTAACATATGCAAGTCAATAA10120.29863166498956856No Hit
CGTAAACGCCGGGTGGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCACCT9830.29007403822603345No Hit
CGCAAACGCCGGGAGAGTTAAAAACGCCGGGGGGAATTCTCTTGCTTCAACA9320.27502441874533384No Hit
AATAAACGCCGGGACGGGAGACATCTCATATGTACCCCGAATTGAGTAAATT9140.2697127883403811No Hit
CCACAACGCCGGTATCCACCAGGGACTAGGACTAGGAGGCTATAGTTCTGTC9060.2673520637159576No Hit
TCACAACGCCGGGAGAGTTGTCAACGCGGGGCGGGCGCCTGTAGTGCCAGCT8490.2505319007669404No Hit
AAACAACGCCGGTCTGTTTCTGATTCACATTGCTAAAGAACCACAGTGGATA8370.2469908138303052No Hit
GTTAAACGCCGGGTGCTCCCCAAGTTAAGAAAATGAGATGTTTTATATAAGC7980.2354822812862408No Hit
ACATAACGCCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC7820.2307608320373939No Hit
CACAAACGCCGGGGAAACCTAGAAGAAAGAGATAAGTTCCTAGACACTTCTA7440.2195473900713824No Hit
GTACAACGCCGGGAGAATAGTCAAACTCATAGAAACGGAAAGTACAATGGTG7200.212465216198112No Hit
CTAGAACGCCGGGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATA7040.20774376694926508No Hit
GGCCAACGCCGGGGTATCAACGGGGCACAAGCCTTGTCCTGTCCTGGGTTCT7040.20774376694926508No Hit
TACCAACGCCGGGCGTTTTTGATGTAAATATTAAGAACTTGGTCTATTTCTG6590.1944646909368831No Hit
GCTTAACGCCGGGGGAAGAGCTAGCATCTCTGACGAAAAACAGCAGACGGAA6230.18384143012697748No Hit
ATTCAACGCCGGGATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT6060.17882489030007762No Hit
GAATAACGCCCGGCATATATATGTATCTTTTTGTTTTTTTGAGATGTAGTCC5940.1752838033634424No Hit
CACAAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT5780.17056235411459547No Hit
CTACAACGCCGGGAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCG5660.1670212671779603No Hit
TTCCAACGCCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC5520.1628899990852192No Hit
GTATAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT5290.15610291579000174No Hit
CTCTAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT5220.1540372817436312No Hit
GAATAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC5010.1478403796045196No Hit
AGCCAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT4980.1469551078703608No Hit
AACCAACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4880.14400420208983147No Hit
ACACAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT4540.13397112243603174No Hit
TCGAAACGCCGGGAAGCAAACACACCAAAAACAAACCAATACTAAACTCCCT4290.1265938579847084No Hit
TTTCAACGCCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4220.12452822393833787No Hit
TGCTAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC4200.12393804278223201No Hit
TTTCAACGCCGGGCTGCTGGCCGTGGGCGGACTCGGCTTCGTGATGACACTG4200.12393804278223201No Hit
GAATAACGCCGGGACTCCAGCCTGGGTAACAGAGCAAGACTCTGTCTCAAAA4050.11951168411143802No Hit
AGGCAACGCCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4040.11921659353338508No Hit
CCTGAACGCCAGGGTTAGAACTTATTTCCAAGTCAAAAATTAGTATCTCCAT3910.11538041601869695No Hit
AGGCAACGCCGGGATACTGTTGGTCTTTACAATTCACCTCATCTTATAGATT3730.11006878561374414No Hit
GCACAACGCCGGGTATGACCTAAAGTAGCACTGTTTCAGCCAACCCATGAGA3580.10564242694295013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCTA700.046.00000440
TTTTGAT700.046.00000417
CATGATT351.0167241E-746.00000441
CATGAGA351.0167241E-746.00000446
CAGTTAA351.0167241E-746.00000434
TAGTGCC1400.046.00000442
TTACCTA351.0167241E-746.00000422
TGACCTA351.0167241E-746.00000416
ATGATTT351.0167241E-746.00000442
CGCCTGT1400.046.00000436
TCCTAGT351.0167241E-746.00000434
TCCTACA700.046.00000435
CCTGTAG1400.046.00000438
GTACTAC351.0167241E-746.00000434
CCTAAAG351.0167241E-746.00000419
TAAAACT351.0167241E-746.00000427
GATGTAA700.046.00000421
GTTTTTG700.046.00000415
TATGACC351.0167241E-746.00000414
AGAACAA351.0167241E-746.00000443
Produced by FastQC (version 0.11.5)