##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528121_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338879
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.075457611713915	31.0	31.0	34.0	28.0	34.0
2	31.73998683896022	31.0	31.0	34.0	30.0	34.0
3	31.909737693985168	31.0	31.0	34.0	30.0	34.0
4	35.637903204388586	37.0	35.0	37.0	33.0	37.0
5	35.70674783624834	37.0	35.0	37.0	35.0	37.0
6	35.88802197834625	37.0	35.0	37.0	35.0	37.0
7	35.46201446534014	37.0	35.0	37.0	33.0	37.0
8	35.178314383600046	37.0	35.0	37.0	32.0	37.0
9	36.36957734176505	38.0	35.0	39.0	31.0	39.0
10	37.13864830809817	39.0	37.0	39.0	32.0	39.0
11	37.60690689006991	39.0	37.0	39.0	35.0	39.0
12	37.52663930193373	39.0	37.0	39.0	35.0	39.0
13	37.34710324334054	39.0	37.0	39.0	35.0	39.0
14	37.215770230672305	40.0	37.0	40.0	31.0	40.0
15	36.45861797278675	39.0	36.0	40.0	30.0	40.0
16	36.17039120157933	38.0	35.0	40.0	30.0	40.0
17	36.1681072005052	38.0	35.0	40.0	30.0	40.0
18	36.04100283582046	38.0	35.0	40.0	30.0	40.0
19	36.37358762272079	38.0	35.0	40.0	30.0	40.0
20	36.60347793755293	39.0	35.0	40.0	30.0	40.0
21	36.689535202830506	39.0	35.0	40.0	31.0	40.0
22	36.60776560365204	39.0	35.0	40.0	30.0	40.0
23	36.66495120677292	39.0	35.0	40.0	31.0	40.0
24	36.60446058917785	39.0	35.0	40.0	31.0	40.0
25	36.38982055541948	38.0	35.0	40.0	30.0	40.0
26	35.81887340319111	38.0	35.0	40.0	30.0	40.0
27	35.49391080592188	38.0	34.0	40.0	29.0	40.0
28	35.381437032097004	38.0	34.0	40.0	28.0	40.0
29	35.35507363985376	37.0	34.0	40.0	28.0	40.0
30	34.959215531207306	37.0	33.0	40.0	26.0	40.0
31	34.84953921606237	37.0	33.0	40.0	26.0	40.0
32	34.70792229674899	36.0	33.0	40.0	26.0	40.0
33	34.60041489735274	36.0	33.0	40.0	26.0	40.0
34	34.368252385069596	35.0	33.0	39.0	26.0	40.0
35	33.91931928505455	35.0	33.0	39.0	24.0	40.0
36	33.58339997462221	35.0	33.0	38.0	23.0	40.0
37	33.21819882612968	35.0	33.0	37.0	23.0	40.0
38	32.76696992141738	35.0	33.0	37.0	21.0	40.0
39	32.2210907137946	35.0	32.0	36.0	20.0	40.0
40	31.712366360854464	35.0	31.0	36.0	18.0	40.0
41	31.14472422310028	35.0	31.0	35.0	15.0	39.0
42	30.5836744088598	35.0	30.0	35.0	12.0	39.0
43	30.061358183894548	35.0	28.0	35.0	10.0	39.0
44	29.470153063482837	34.0	25.0	35.0	9.0	38.0
45	29.087458945523327	34.0	24.0	35.0	9.0	38.0
46	28.602920216360413	34.0	23.0	35.0	8.0	37.0
47	28.18584804605774	33.0	23.0	35.0	8.0	37.0
48	27.75943920986547	33.0	21.0	35.0	8.0	36.0
49	27.236355749397276	33.0	20.0	35.0	8.0	36.0
50	26.783114326942655	33.0	18.0	35.0	8.0	35.0
51	26.358889161027978	33.0	17.0	35.0	8.0	35.0
52	25.4775391806515	32.0	12.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	6.0
15	16.0
16	38.0
17	115.0
18	252.0
19	580.0
20	1036.0
21	1727.0
22	2515.0
23	3577.0
24	4763.0
25	6223.0
26	8134.0
27	10504.0
28	12984.0
29	15819.0
30	17384.0
31	17752.0
32	17980.0
33	20382.0
34	25563.0
35	36458.0
36	50490.0
37	58436.0
38	24200.0
39	1943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.14031852076995	25.261228934221357	37.454371619368565	6.144080925640125
2	27.92412631057103	22.012576760436616	32.36789532547015	17.6954016035222
3	17.868619772839274	25.563401686147564	32.68629805918927	23.881680481823896
4	17.65202328854842	26.122008150401765	30.24501370695735	25.980954854092463
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	0.0	100.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	0.0	100.0
11	97.162409001443	1.6687372188893383	0.22279338642996466	0.9460603932377043
12	96.03781880848327	3.4124274446041216	0.2906642193821393	0.2590895275304755
13	89.92442730296064	7.011942315693802	2.0980940099563563	0.9655363713891979
14	39.546268727185804	37.88638422563806	14.414584556729688	8.152762490446444
15	35.409098822883685	35.8836044723928	20.355938255247448	8.351358449476066
16	32.79282575786638	36.92232330713912	18.08256044192765	12.202290493066847
17	27.408603070712555	30.86706464549294	19.9386801778806	21.78565210591391
18	16.219063441523378	38.72975309771334	28.012063302830803	17.039120157932476
19	19.89235095712629	42.75508367293341	25.824556847724413	11.528008522215895
20	21.00071116829311	39.99893767391901	23.067525576975854	15.932825580812029
21	21.601220494630827	40.47344332342813	15.216050566721457	22.709285615219592
22	21.87506455106395	43.42110310759888	17.05239923394486	17.651433107392315
23	17.605398977216055	47.12124386580461	17.617497690916224	17.65585946606311
24	16.76852209785794	45.863272731564955	14.492783559913716	22.875421610663395
25	25.014238120391052	37.152493958020415	13.891979142997943	23.94128877859059
26	24.78613310355614	37.60073654608282	12.77889748258228	24.834232867778766
27	18.527261943053418	45.59326485264652	11.981562740683254	23.897910463616807
28	17.333620554829306	46.40446885171403	11.572567199501888	24.689343393954776
29	26.178075360231823	47.27970750621903	11.387545407062698	15.154671726486447
30	26.334768457177933	51.570619601686744	11.049666695192089	11.044945245943243
31	20.48577810959074	54.86796172085021	10.51850365469681	14.127756514862236
32	17.564971568022806	57.977921322950074	12.117304406587602	12.339802702439513
33	13.985522856240722	61.246934746620475	13.23363206336185	11.533910333776953
34	14.521112255406798	62.936623396551575	11.570796656033568	10.971467692008062
35	16.543663077381602	61.35965934743669	12.240062087057622	9.85661548812408
36	15.10775232457603	59.50560524553011	13.079004600462113	12.307637829431744
37	13.056577716530088	60.59065330103075	12.139436199941573	14.213332782497586
38	13.852732096116904	58.01097146769201	13.575051862169094	14.561244574021995
39	14.358812437477683	58.63892421778865	13.181991212202584	13.82027213253108
40	14.919484535778258	57.99887275399184	13.23687805972043	13.844764650509475
41	16.209915633603735	59.264516243260864	11.81926292275414	12.706305200381257
42	15.017749698269883	56.866020024846634	14.019163182138758	14.09706709474473
43	17.19817397950301	54.80215652194441	14.519931893094585	13.479737605457995
44	19.584276393639026	55.45106070308281	12.433051325104241	12.531611578173921
45	17.94209732677445	53.16764981010921	12.272817141221498	16.617435721894836
46	18.06249428262005	55.983994287046414	11.771458249109564	14.182053181223978
47	17.74261609601067	54.88714260842366	12.823751250446325	14.54649004511935
48	18.933306578454257	54.63779106996892	13.240714237235117	13.188188114341697
49	18.56031208779535	57.52731801026325	12.8565063046102	11.0558635973312
50	17.40149138778148	57.16789768619478	12.430100419323711	13.00051050670003
51	17.323587475175508	54.79389398575892	14.347894086089724	13.53462445297584
52	19.42964893073929	52.65950383470206	14.299499231289046	13.611348003269605
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	24.0
10	45.0
11	352.0
12	659.0
13	666.5
14	1532.0
15	2390.0
16	2653.0
17	2916.0
18	2829.0
19	2742.0
20	3042.0
21	3342.0
22	3331.5
23	3321.0
24	4276.0
25	5231.0
26	7375.5
27	9520.0
28	13164.0
29	16808.0
30	19976.5
31	23145.0
32	23762.0
33	24379.0
34	23519.5
35	22660.0
36	20370.0
37	18080.0
38	17334.0
39	15067.0
40	13546.0
41	12439.5
42	11333.0
43	12040.0
44	12747.0
45	12331.5
46	11916.0
47	15236.5
48	18557.0
49	17372.5
50	16188.0
51	16222.0
52	16256.0
53	18421.0
54	20586.0
55	19297.5
56	18009.0
57	15809.5
58	13610.0
59	12464.5
60	11319.0
61	8863.0
62	6407.0
63	6708.0
64	5325.5
65	3642.0
66	3709.5
67	3777.0
68	2407.0
69	1037.0
70	613.0
71	189.0
72	134.5
73	80.0
74	83.5
75	87.0
76	63.0
77	39.0
78	32.0
79	25.0
80	18.5
81	12.0
82	8.0
83	4.0
84	2.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	338879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.20457444991753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.86516251928582	38.84042118137872
2	5.185261477936615	4.58424554087477
3	1.5696613400777029	2.0815863470586637
4	0.7763947896700267	1.3728080512998702
5	0.49682405488017145	1.0980947961230227
6	0.3703924916203453	0.9823825482913205
7	0.2805594720269647	0.8681408448191862
8	0.2280791742571986	0.8065714271142438
9	0.2380929735680686	0.9472318718482741
>10	2.650915878646218	25.09830513616935
>50	0.23605317122150737	6.910867294215457
>100	0.08114327760739316	7.508487011332017
>500	0.014753323201344211	4.625327602266709
>1k	0.0067060560006110045	4.275530347208405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAACGCCGGGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAAGC	2025	0.5975584205571901	No Hit
TTCAAACGCCGAGAGACCCAACCTTCGGGAGGCATCACGCTTTAGACTACCA	1950	0.57542662720322	No Hit
ACCGAACGCCGGGTAACACCGGGGTCTTATTCTTAGTCATTCAGGAAGTTTT	1805	0.5326384933855447	No Hit
CTGCAACGCCGGAGACAGAAAAAGGCCAGGCGCGGTGGCTCATGCCCATAAT	1531	0.451783674999041	No Hit
GGACAACGCCGGGAGAGTGGGTAACGCCGGGAAAACCCCCAACACTCCGATG	1368	0.4036839107764128	No Hit
GTTGAACGCCGGGGGGCCAACATAGTGAAACCCCATCTCTACTAAAAAATAC	1333	0.39335574054456013	No Hit
AGTTAACGCCGGGAGAGTTTGAAACGCCGGGGCATTTTCGCTATTGTTCAAT	1175	0.3467314292121967	No Hit
AGTTAACGCCCGGGCAGCACTTTGGGAGGCCGAGGCAGGAGGATCACCTGAG	1144	0.33758362129255576	No Hit
ATAGAACGCCGGGGGTACACAATTGCCTATAATGAGCAAAATTGAATTAGGA	1080	0.31869782429716803	No Hit
CTAGAACGCCGGGCAGGGATGCTGGGATGGTTTAACATATGCAAGTCAATAA	1012	0.29863166498956856	No Hit
CGTAAACGCCGGGTGGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCACCT	983	0.29007403822603345	No Hit
CGCAAACGCCGGGAGAGTTAAAAACGCCGGGGGGAATTCTCTTGCTTCAACA	932	0.27502441874533384	No Hit
AATAAACGCCGGGACGGGAGACATCTCATATGTACCCCGAATTGAGTAAATT	914	0.2697127883403811	No Hit
CCACAACGCCGGTATCCACCAGGGACTAGGACTAGGAGGCTATAGTTCTGTC	906	0.2673520637159576	No Hit
TCACAACGCCGGGAGAGTTGTCAACGCGGGGCGGGCGCCTGTAGTGCCAGCT	849	0.2505319007669404	No Hit
AAACAACGCCGGTCTGTTTCTGATTCACATTGCTAAAGAACCACAGTGGATA	837	0.2469908138303052	No Hit
GTTAAACGCCGGGTGCTCCCCAAGTTAAGAAAATGAGATGTTTTATATAAGC	798	0.2354822812862408	No Hit
ACATAACGCCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC	782	0.2307608320373939	No Hit
CACAAACGCCGGGGAAACCTAGAAGAAAGAGATAAGTTCCTAGACACTTCTA	744	0.2195473900713824	No Hit
GTACAACGCCGGGAGAATAGTCAAACTCATAGAAACGGAAAGTACAATGGTG	720	0.212465216198112	No Hit
CTAGAACGCCGGGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATA	704	0.20774376694926508	No Hit
GGCCAACGCCGGGGTATCAACGGGGCACAAGCCTTGTCCTGTCCTGGGTTCT	704	0.20774376694926508	No Hit
TACCAACGCCGGGCGTTTTTGATGTAAATATTAAGAACTTGGTCTATTTCTG	659	0.1944646909368831	No Hit
GCTTAACGCCGGGGGAAGAGCTAGCATCTCTGACGAAAAACAGCAGACGGAA	623	0.18384143012697748	No Hit
ATTCAACGCCGGGATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT	606	0.17882489030007762	No Hit
GAATAACGCCCGGCATATATATGTATCTTTTTGTTTTTTTGAGATGTAGTCC	594	0.1752838033634424	No Hit
CACAAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	578	0.17056235411459547	No Hit
CTACAACGCCGGGAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCG	566	0.1670212671779603	No Hit
TTCCAACGCCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC	552	0.1628899990852192	No Hit
GTATAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	529	0.15610291579000174	No Hit
CTCTAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	522	0.1540372817436312	No Hit
GAATAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC	501	0.1478403796045196	No Hit
AGCCAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	498	0.1469551078703608	No Hit
AACCAACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	488	0.14400420208983147	No Hit
ACACAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	454	0.13397112243603174	No Hit
TCGAAACGCCGGGAAGCAAACACACCAAAAACAAACCAATACTAAACTCCCT	429	0.1265938579847084	No Hit
TTTCAACGCCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC	422	0.12452822393833787	No Hit
TGCTAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC	420	0.12393804278223201	No Hit
TTTCAACGCCGGGCTGCTGGCCGTGGGCGGACTCGGCTTCGTGATGACACTG	420	0.12393804278223201	No Hit
GAATAACGCCGGGACTCCAGCCTGGGTAACAGAGCAAGACTCTGTCTCAAAA	405	0.11951168411143802	No Hit
AGGCAACGCCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC	404	0.11921659353338508	No Hit
CCTGAACGCCAGGGTTAGAACTTATTTCCAAGTCAAAAATTAGTATCTCCAT	391	0.11538041601869695	No Hit
AGGCAACGCCGGGATACTGTTGGTCTTTACAATTCACCTCATCTTATAGATT	373	0.11006878561374414	No Hit
GCACAACGCCGGGTATGACCTAAAGTAGCACTGTTTCAGCCAACCCATGAGA	358	0.10564242694295013	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0734775539351804	0.0	0.0	0.0	0.0
12	0.1159705971748028	0.0	0.0	0.0	0.0
13	0.14518456440204322	0.0	0.0	0.0	0.0
14	0.2652864296695871	0.0	0.0	0.0	0.0
15	0.3992575521056188	0.0	0.0	0.0	0.0
16	0.5822137104984375	0.0	0.0	0.0	0.0
17	0.7312344524151688	0.0	0.0	0.0	0.0
18	0.8947146326564939	0.0	0.0	0.0	0.0
19	1.1057043959643413	0.0	0.0	0.0	0.0
20	1.3346946845334176	0.0	0.0	0.0	0.0
21	1.5666358788830232	0.0	0.0	0.0	0.0
22	1.7817569102836115	0.0	0.0	0.0	0.0
23	1.9871399526084532	0.0	0.0	0.0	0.0
24	2.1875064551063947	0.0	0.0	0.0	0.0
25	2.40026676188256	0.0	0.0	0.0	0.0
26	2.668209006754623	0.0	0.0	0.0	0.0
27	2.9119538242263463	0.0	0.0	0.0	0.0
28	3.1716335329129275	0.0	0.0	0.0	0.0
29	3.4823639116026666	0.0	0.0	0.0	0.0
30	3.8807361919741266	0.0	0.0	0.0	0.0
31	4.2537306826330346	0.0	0.0	0.0	0.0
32	4.523738561551468	0.0	0.0	0.0	0.0
33	4.759220842837709	0.0	0.0	0.0	0.0
34	4.957226620711228	0.0	0.0	0.0	0.0
35	5.161134210145804	0.0	0.0	0.0	0.0
36	5.367107433626752	0.0	0.0	0.0	0.0
37	5.597868265664146	0.0	0.0	0.0	0.0
38	5.813874568798893	0.0	0.0	0.0	0.0
39	6.050537212397345	0.0	0.0	0.0	0.0
40	6.306675834147292	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTCTA	70	0.0	46.000004	40
TTTTGAT	70	0.0	46.000004	17
CATGATT	35	1.0167241E-7	46.000004	41
CATGAGA	35	1.0167241E-7	46.000004	46
CAGTTAA	35	1.0167241E-7	46.000004	34
TAGTGCC	140	0.0	46.000004	42
TTACCTA	35	1.0167241E-7	46.000004	22
TGACCTA	35	1.0167241E-7	46.000004	16
ATGATTT	35	1.0167241E-7	46.000004	42
CGCCTGT	140	0.0	46.000004	36
TCCTAGT	35	1.0167241E-7	46.000004	34
TCCTACA	70	0.0	46.000004	35
CCTGTAG	140	0.0	46.000004	38
GTACTAC	35	1.0167241E-7	46.000004	34
CCTAAAG	35	1.0167241E-7	46.000004	19
TAAAACT	35	1.0167241E-7	46.000004	27
GATGTAA	70	0.0	46.000004	21
GTTTTTG	70	0.0	46.000004	15
TATGACC	35	1.0167241E-7	46.000004	14
AGAACAA	35	1.0167241E-7	46.000004	43
>>END_MODULE