SRR1528120_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528120_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	327528
Sequences flagged as poor quality	0
Sequence length	59
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCTAACGCCGGGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAAGCGGCAGCG	1760	0.5373586380401065	No Hit
TTCAAACGCCGAGAGACCCAACCTTCGGGAGGCATCACGCTTTAGACTACCACGTTAAA	1645	0.5022471361227132	No Hit
ACCGAACGCCGGGTAACACCGGGGTCTTATTCTTAGTCATTCAGGAAGTTTTCTTGTGT	1405	0.42897095820815323	No Hit
CTGCAACGCCGGAGACAGAAAAAGGCCAGGCGCGGTGGCTCATGCCCATAATCCCAGCA	1375	0.41981143596883325	No Hit
GTTGAACGCCGGGGGGCCAACATAGTGAAACCCCATCTCTACTAAAAAATACAAAAATT	1141	0.34836716250213723	No Hit
GGACAACGCCGGGAGAGTGGGTAACGCCGGGAAAACCCCCAACACTCCGATGCGGGTGA	1095	0.3343225617351799	No Hit
ATAGAACGCCGGGGGTACACAATTGCCTATAATGAGCAAAATTGAATTAGGAGACGATG	1071	0.3269949439437239	No Hit
AGTTAACGCCGGGAGAGTTTGAAACGCCGGGGCATTTTCGCTATTGTTCAATTAATTTT	1034	0.31569819984856257	No Hit
AGTTAACGCCCGGGCAGCACTTTGGGAGGCCGAGGCAGGAGGATCACCTGAGGTCAGGA	933	0.2848611416428519	No Hit
CTAGAACGCCGGGCAGGGATGCTGGGATGGTTTAACATATGCAAGTCAATAAATATGAT	887	0.2708165408758946	No Hit
CCACAACGCCGGTATCCACCAGGGACTAGGACTAGGAGGCTATAGTTCTGTCTGACATA	871	0.2659314623482572	No Hit
CGTAAACGCCGGGTGGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCACCTGACCTCG	829	0.25310813121320924	No Hit
AAACAACGCCGGTCTGTTTCTGATTCACATTGCTAAAGAACCACAGTGGATATGTTTGT	803	0.2451698786057986	No Hit
CGCAAACGCCGGGAGAGTTAAAAACGCCGGGGGGAATTCTCTTGCTTCAACAATAACGT	761	0.23234654747075056	No Hit
CACAAACGCCGGGGAAACCTAGAAGAAAGAGATAAGTTCCTAGACACTTCTATTCAACA	738	0.22532424708727192	No Hit
AATAAACGCCGGGACGGGAGACATCTCATATGTACCCCGAATTGAGTAAATTCAGTTAG	713	0.21769131188783858	No Hit
TCACAACGCCGGGAGAGTTGTCAACGCGGGGCGGGCGCCTGTAGTGCCAGCTACTCGGG	690	0.21066901150435993	No Hit
GTTAAACGCCGGGTGCTCCCCAAGTTAAGAAAATGAGATGTTTTATATAAGCGGGTATC	682	0.20822647224054128	No Hit
GTACAACGCCGGGAGAATAGTCAAACTCATAGAAACGGAAAGTACAATGGTGCTTGCCA	675	0.2060892503846999	No Hit
TACCAACGCCGGGCGTTTTTGATGTAAATATTAAGAACTTGGTCTATTTCTGCAAGTCA	657	0.20059353704110794	No Hit
CTAGAACGCCGGGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGA	606	0.18502234923426394	No Hit
ACATAACGCCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA	600	0.18319044478639995	No Hit
GGCCAACGCCGGGGTATCAACGGGGCACAAGCCTTGTCCTGTCCTGGGTTCTTCCTTAC	539	0.1645660828997826	No Hit
CACAAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA	531	0.16212354363596396	No Hit
GTATAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA	520	0.15876505214821326	No Hit
CTACAACGCCGGGAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATA	514	0.1569331477003493	No Hit
GCTTAACGCCGGGGGAAGAGCTAGCATCTCTGACGAAAAACAGCAGACGGAAAAGTACT	505	0.15418529102855327	No Hit
GAATAACGCCCGGCATATATATGTATCTTTTTGTTTTTTTGAGATGTAGTCCCACTTCT	488	0.14899489509293862	No Hit
ATTCAACGCCGGGATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATAT	463	0.14136195989350528	No Hit
AGCCAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA	435	0.13281307247013996	No Hit
TTCCAACGCCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGG	416	0.12701204171857064	No Hit
CTCTAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA	415	0.1267067243105933	No Hit
ACACAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA	414	0.12640140690261595	No Hit
GAATAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATC	399	0.12182164578295597	No Hit
GAATAACGCCGGGACTCCAGCCTGGGTAACAGAGCAAGACTCTGTCTCAAAAACAAACA	395	0.12060037615104661	No Hit
TCGAAACGCCGGGAAGCAAACACACCAAAAACAAACCAATACTAAACTCCCTTCTCCAA	387	0.11815783688722797	No Hit
CCTGAACGCCAGGGTTAGAACTTATTTCCAAGTCAAAAATTAGTATCTCCATTAGGGGC	364	0.1111355365037493	No Hit
TGCTAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATC	340	0.10380791871229329	No Hit
AACCAACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	340	0.10380791871229329	No Hit
GCACAACGCCGGGTATGACCTAAAGTAGCACTGTTTCAGCCAACCCATGAGACCATTAG	334	0.1019760142644293	No Hit
TTTCAACGCCGGGCTGCTGGCCGTGGGCGGACTCGGCTTCGTGATGACACTGCTCTACA	330	0.10075474463251996	No Hit
AGGCAACGCCGGGATACTGTTGGTCTTTACAATTCACCTCATCTTATAGATTTTACTGA	328	0.1001441098165653	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGGTTTA	135	53.000004	28
GGATGCT	135	53.000004	17
TTCACCG	75	53.000004	19
AAGTACT	75	53.000004	53
GGCATTT	150	53.000004	31
ATCTCTG	75	53.000004	26
TAAATTC	75	53.000004	47
TTCGCTA	150	53.000004	37
GAAACCT	75	53.000004	14
TTCAATT	150	53.000004	47
TTTCTTG	150	53.000004	50
TCCTAGA	75	53.000004	38
TAAGTTC	75	53.000004	33
CTGGGAT	135	53.000004	22
TATGTAC	75	53.000004	29
ATGGTTT	135	53.000004	27
ATGGTCT	75	53.000004	37
AATTAAT	150	53.000004	50
TTTCACC	75	53.000004	18
CAATTAA	150	53.000004	49

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content