##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1528120_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 327528 Sequences flagged as poor quality 0 Sequence length 59 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.578426882587138 31.0 31.0 33.0 27.0 34.0 2 31.341308224029703 31.0 31.0 34.0 30.0 34.0 3 31.547101316528664 31.0 31.0 34.0 30.0 34.0 4 35.32606677902347 37.0 35.0 37.0 33.0 37.0 5 35.217706577758236 37.0 35.0 37.0 33.0 37.0 6 35.53117290735449 37.0 35.0 37.0 33.0 37.0 7 34.97320534427591 37.0 35.0 37.0 32.0 37.0 8 34.521323367773135 35.0 35.0 37.0 30.0 37.0 9 35.786842651620624 38.0 35.0 39.0 30.0 39.0 10 36.81768886934857 39.0 37.0 39.0 32.0 39.0 11 37.061909210815564 39.0 37.0 39.0 34.0 39.0 12 37.06697442661391 39.0 37.0 39.0 34.0 39.0 13 36.8430149483403 39.0 37.0 39.0 32.0 39.0 14 36.29450306538678 39.0 36.0 40.0 30.0 40.0 15 35.515119318043034 38.0 34.0 40.0 27.0 40.0 16 35.04782186561149 38.0 33.0 40.0 26.0 40.0 17 35.251706724310594 38.0 33.0 40.0 26.0 40.0 18 35.289306563103004 38.0 33.0 40.0 26.0 40.0 19 35.71969114091009 38.0 34.0 40.0 29.0 40.0 20 35.81925209447742 38.0 34.0 40.0 29.0 40.0 21 35.84927395520383 38.0 34.0 40.0 29.0 40.0 22 35.72539752326519 38.0 34.0 40.0 29.0 40.0 23 35.68813963996971 38.0 34.0 40.0 28.0 40.0 24 35.61949512713417 38.0 34.0 40.0 28.0 40.0 25 35.43613675777338 37.0 34.0 40.0 28.0 40.0 26 34.709978994162334 37.0 33.0 40.0 26.0 40.0 27 34.41409284091742 36.0 33.0 40.0 25.0 40.0 28 34.24467831757895 36.0 33.0 40.0 24.0 40.0 29 34.08448437996141 36.0 33.0 40.0 24.0 40.0 30 33.69867919689309 35.0 32.0 39.0 22.0 40.0 31 33.56549363718522 35.0 32.0 39.0 22.0 40.0 32 33.30686536723578 35.0 32.0 39.0 22.0 40.0 33 33.05193143792287 35.0 32.0 39.0 21.0 40.0 34 32.793855792481864 35.0 31.0 38.0 21.0 40.0 35 32.528189956278545 35.0 31.0 38.0 21.0 40.0 36 32.01149825358443 35.0 31.0 37.0 20.0 40.0 37 31.792252876089982 35.0 31.0 37.0 20.0 40.0 38 31.457893676265847 35.0 30.0 36.0 18.0 39.0 39 30.848498448987566 34.0 29.0 35.0 15.0 39.0 40 30.372114750494614 34.0 28.0 35.0 15.0 39.0 41 29.768126694511615 33.0 26.0 35.0 11.0 39.0 42 29.284149752082264 33.0 25.0 35.0 10.0 38.0 43 28.77167448279231 33.0 23.0 35.0 9.0 38.0 44 28.245713343591998 33.0 22.0 35.0 9.0 37.0 45 27.940835592682152 33.0 22.0 35.0 8.0 37.0 46 27.46643951051513 33.0 20.0 35.0 8.0 36.0 47 26.94158056715762 33.0 20.0 35.0 8.0 36.0 48 26.568644512835544 33.0 20.0 35.0 8.0 35.0 49 26.093262255440756 32.0 17.0 35.0 8.0 35.0 50 25.488492586893333 31.0 15.0 35.0 8.0 35.0 51 25.058538506631493 31.0 12.0 35.0 8.0 35.0 52 24.348483183117168 30.0 10.0 34.0 8.0 35.0 53 24.040750714442733 29.0 10.0 34.0 8.0 35.0 54 23.54732114506241 28.0 10.0 34.0 8.0 35.0 55 23.04326347671039 27.0 9.0 34.0 8.0 35.0 56 22.412871571285507 25.0 9.0 34.0 8.0 35.0 57 21.703036076304926 23.0 8.0 33.0 8.0 35.0 58 21.157272050023206 22.0 8.0 33.0 8.0 35.0 59 20.38335959063042 20.0 8.0 33.0 7.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 7.0 14 21.0 15 47.0 16 168.0 17 463.0 18 1065.0 19 2016.0 20 3161.0 21 4476.0 22 6248.0 23 8122.0 24 10507.0 25 13094.0 26 15624.0 27 17716.0 28 17894.0 29 16732.0 30 17812.0 31 19933.0 32 22748.0 33 25348.0 34 29323.0 35 32433.0 36 34779.0 37 20990.0 38 6668.0 39 131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.05352824796659 26.238062089348084 36.674421728829294 6.033987933856037 2 27.45811045162551 22.680503651596197 32.10198822696075 17.75939766981754 3 17.898622407855207 26.014875064116655 32.46317872059794 23.623323807430204 4 17.432402725873818 26.68168828313915 30.035600009770157 25.850308981216873 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 0.0 0.0 0.0 100.0 8 100.0 0.0 0.0 0.0 9 0.0 0.0 0.0 100.0 10 0.0 0.0 0.0 100.0 11 97.25336459783591 1.6450501941818716 0.2088371070564959 0.8927481009257224 12 96.09834884345766 3.3438362521677534 0.2830292371949879 0.2747856671795999 13 90.17183263720965 6.817432402725873 2.0718839305341836 0.9388510295302996 14 39.34564373122298 38.195513055372366 14.277557949244034 8.181285264160621 15 35.39788964607606 36.31811631371974 20.038897437776313 8.245096602427884 16 32.29311692435456 37.296658606287096 18.183788866906035 12.226435602452309 17 26.95128355438314 31.303277887692044 19.97508609950905 21.770352458415772 18 15.99893749542024 39.04124227546958 27.87273149165873 17.087088737451456 19 19.877079211548327 42.79756234581471 25.90801397132459 11.417344471312378 20 21.041254488165897 39.99139004909504 23.089628978285827 15.877726484453236 21 21.45679148042305 40.882916880388855 15.409369580616008 22.250922058572094 22 21.755697222832858 43.59871522434723 17.18234776874038 17.46323978407953 23 17.269363230013923 47.51685352092035 17.802447424342347 17.41133582472338 24 16.49141447448768 46.58441415695757 14.558144647175203 22.366026721379544 25 24.716970762805012 37.5390806282211 13.77195232163357 23.971996287340318 26 24.575608802911507 38.119489020786006 12.617241884663297 24.687660291639187 27 18.202107911384676 45.888901101585205 11.824027258738184 24.08496372829193 28 16.969236217972202 46.826836178891575 11.27506655919494 24.928861043941282 29 26.23867272416404 47.495786619769916 10.981656530128722 15.283884125937325 30 26.271952321633567 51.92166776580933 10.662599838792408 11.143780073764686 31 20.39642412251777 55.479836838377175 10.391783297916515 13.73195574118854 32 17.21471141398598 58.77757016194035 11.77761901272563 12.230099411348037 33 13.864158178842725 62.153159424537755 12.988202535355756 10.99447986126377 34 14.05376028919665 63.996665933904886 11.256747514716299 10.692826262182164 35 16.385164016511567 62.387032559048386 11.693046090715908 9.534757333724139 36 14.887276812974767 60.39544710681224 12.842260814342591 11.875015265870399 37 12.563200703451308 61.845704794704574 11.59900832905889 13.992086172785228 38 13.392442783517744 59.402860213477936 13.035526733592242 14.169170269412081 39 13.832710485821059 59.99883979384969 12.653269338804622 13.515180381524633 40 14.357245792726117 59.42087394054859 12.624569502454753 13.597310764270535 41 15.625534305463962 60.73557069929899 11.352006546005226 12.28688844923182 42 14.328851273784228 58.582472338242845 13.193681150924503 13.894995237048436 43 16.66727730148262 56.27671527319802 13.738062089348086 13.317945335971276 44 18.913497471971862 56.88460223248089 11.717776800762072 12.484123494785178 45 17.220817762145526 54.568464375564844 11.566644683813292 16.644073178476344 46 17.57529127280721 57.12885615886275 11.274761241786962 14.021091326543075 47 17.22753474512103 55.989716909699325 12.376651767177158 14.406096578002492 48 18.222564177719157 55.80805305195281 12.8044014557534 13.16498131457463 49 18.15111990425246 58.663381451356834 12.271011944017001 10.91448670037371 50 16.80161696099265 58.40722014606384 11.791968930900564 12.99919396204294 51 16.5692704135219 56.20008060379571 13.924305708214261 13.306343274468139 52 16.891685596345962 55.357709875186245 14.219852959136317 13.530751569331478 53 16.76436823721941 53.993246378935545 13.201619403531911 16.040765980313136 54 17.532546835690383 53.964546542585666 14.219547641728342 14.283358979995603 55 17.56918492464766 52.63183605676461 13.737451454532131 16.06152756405559 56 18.805415109547884 50.317530104296424 15.93817933123275 14.938875454922936 57 20.533511638699594 48.9439070858064 15.54767836642974 14.974902909064264 58 21.731882465010624 45.631518526660315 14.511736401162647 18.12486260716641 59 21.815539434796413 46.11544661830439 16.886800517818322 15.182213429080873 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 12.5 9 258.0 10 493.0 11 516.0 12 539.0 13 1122.5 14 1887.5 15 2069.0 16 1995.5 17 1922.0 18 1967.0 19 1760.0 20 1508.0 21 1673.5 22 1839.0 23 2409.5 24 2980.0 25 4402.0 26 7699.5 27 9575.0 28 11850.0 29 14125.0 30 16340.5 31 19843.5 32 21131.0 33 20865.5 34 20600.0 35 19931.5 36 17964.0 37 16665.0 38 15777.5 39 14890.0 40 15139.0 41 15388.0 42 16897.5 43 16942.5 44 15478.0 45 14930.5 46 14383.0 47 15050.0 48 14353.5 49 12990.0 50 13295.5 51 13601.0 52 12879.0 53 11019.5 54 9882.0 55 9809.0 56 9736.0 57 8685.0 58 7980.5 59 8327.0 60 7171.0 61 6015.0 62 5364.0 63 4713.0 64 4316.5 65 2781.0 66 1642.0 67 1435.0 68 1228.0 69 768.0 70 202.5 71 97.0 72 85.5 73 74.0 74 62.0 75 41.5 76 33.0 77 22.0 78 11.0 79 10.0 80 9.0 81 6.0 82 1.5 83 0.0 84 0.5 85 1.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 59 327528.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.49099049462138 #Duplication Level Percentage of deduplicated Percentage of total 1 93.03081989384658 57.20557261799354 2 3.328704881758621 4.093707204872383 3 0.9961612074433613 1.8376481801403086 4 0.47425769869202694 1.1665030256908977 5 0.3021880114415587 0.9290920069570712 6 0.19772371066171307 0.7294936087716399 7 0.14818899550046574 0.6378601679608641 8 0.12879390194735232 0.6335731680327872 9 0.1153941132492756 0.6386128488851822 >10 1.1112052061551212 14.504475683496349 >50 0.10203821434833557 4.141739513545115 >100 0.05101910717416774 6.229218057910974 >500 0.009503559179501835 4.0150206156110135 >1k 0.004001498601895509 3.2374833001318777 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAACGCCGGGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAAGCGGCAGCG 1760 0.5373586380401065 No Hit TTCAAACGCCGAGAGACCCAACCTTCGGGAGGCATCACGCTTTAGACTACCACGTTAAA 1645 0.5022471361227132 No Hit ACCGAACGCCGGGTAACACCGGGGTCTTATTCTTAGTCATTCAGGAAGTTTTCTTGTGT 1405 0.42897095820815323 No Hit CTGCAACGCCGGAGACAGAAAAAGGCCAGGCGCGGTGGCTCATGCCCATAATCCCAGCA 1375 0.41981143596883325 No Hit GTTGAACGCCGGGGGGCCAACATAGTGAAACCCCATCTCTACTAAAAAATACAAAAATT 1141 0.34836716250213723 No Hit GGACAACGCCGGGAGAGTGGGTAACGCCGGGAAAACCCCCAACACTCCGATGCGGGTGA 1095 0.3343225617351799 No Hit ATAGAACGCCGGGGGTACACAATTGCCTATAATGAGCAAAATTGAATTAGGAGACGATG 1071 0.3269949439437239 No Hit AGTTAACGCCGGGAGAGTTTGAAACGCCGGGGCATTTTCGCTATTGTTCAATTAATTTT 1034 0.31569819984856257 No Hit AGTTAACGCCCGGGCAGCACTTTGGGAGGCCGAGGCAGGAGGATCACCTGAGGTCAGGA 933 0.2848611416428519 No Hit CTAGAACGCCGGGCAGGGATGCTGGGATGGTTTAACATATGCAAGTCAATAAATATGAT 887 0.2708165408758946 No Hit CCACAACGCCGGTATCCACCAGGGACTAGGACTAGGAGGCTATAGTTCTGTCTGACATA 871 0.2659314623482572 No Hit CGTAAACGCCGGGTGGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCACCTGACCTCG 829 0.25310813121320924 No Hit AAACAACGCCGGTCTGTTTCTGATTCACATTGCTAAAGAACCACAGTGGATATGTTTGT 803 0.2451698786057986 No Hit CGCAAACGCCGGGAGAGTTAAAAACGCCGGGGGGAATTCTCTTGCTTCAACAATAACGT 761 0.23234654747075056 No Hit CACAAACGCCGGGGAAACCTAGAAGAAAGAGATAAGTTCCTAGACACTTCTATTCAACA 738 0.22532424708727192 No Hit AATAAACGCCGGGACGGGAGACATCTCATATGTACCCCGAATTGAGTAAATTCAGTTAG 713 0.21769131188783858 No Hit TCACAACGCCGGGAGAGTTGTCAACGCGGGGCGGGCGCCTGTAGTGCCAGCTACTCGGG 690 0.21066901150435993 No Hit GTTAAACGCCGGGTGCTCCCCAAGTTAAGAAAATGAGATGTTTTATATAAGCGGGTATC 682 0.20822647224054128 No Hit GTACAACGCCGGGAGAATAGTCAAACTCATAGAAACGGAAAGTACAATGGTGCTTGCCA 675 0.2060892503846999 No Hit TACCAACGCCGGGCGTTTTTGATGTAAATATTAAGAACTTGGTCTATTTCTGCAAGTCA 657 0.20059353704110794 No Hit CTAGAACGCCGGGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGA 606 0.18502234923426394 No Hit ACATAACGCCGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA 600 0.18319044478639995 No Hit GGCCAACGCCGGGGTATCAACGGGGCACAAGCCTTGTCCTGTCCTGGGTTCTTCCTTAC 539 0.1645660828997826 No Hit CACAAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA 531 0.16212354363596396 No Hit GTATAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA 520 0.15876505214821326 No Hit CTACAACGCCGGGAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATA 514 0.1569331477003493 No Hit GCTTAACGCCGGGGGAAGAGCTAGCATCTCTGACGAAAAACAGCAGACGGAAAAGTACT 505 0.15418529102855327 No Hit GAATAACGCCCGGCATATATATGTATCTTTTTGTTTTTTTGAGATGTAGTCCCACTTCT 488 0.14899489509293862 No Hit ATTCAACGCCGGGATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATAT 463 0.14136195989350528 No Hit AGCCAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA 435 0.13281307247013996 No Hit TTCCAACGCCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGG 416 0.12701204171857064 No Hit CTCTAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA 415 0.1267067243105933 No Hit ACACAACGCCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA 414 0.12640140690261595 No Hit GAATAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATC 399 0.12182164578295597 No Hit GAATAACGCCGGGACTCCAGCCTGGGTAACAGAGCAAGACTCTGTCTCAAAAACAAACA 395 0.12060037615104661 No Hit TCGAAACGCCGGGAAGCAAACACACCAAAAACAAACCAATACTAAACTCCCTTCTCCAA 387 0.11815783688722797 No Hit CCTGAACGCCAGGGTTAGAACTTATTTCCAAGTCAAAAATTAGTATCTCCATTAGGGGC 364 0.1111355365037493 No Hit TGCTAACGCCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATC 340 0.10380791871229329 No Hit AACCAACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 340 0.10380791871229329 No Hit GCACAACGCCGGGTATGACCTAAAGTAGCACTGTTTCAGCCAACCCATGAGACCATTAG 334 0.1019760142644293 No Hit TTTCAACGCCGGGCTGCTGGCCGTGGGCGGACTCGGCTTCGTGATGACACTGCTCTACA 330 0.10075474463251996 No Hit AGGCAACGCCGGGATACTGTTGGTCTTTACAATTCACCTCATCTTATAGATTTTACTGA 328 0.1001441098165653 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.05862094233164798 0.0 0.0 0.0 0.0 12 0.1038079187122933 0.0 0.0 0.0 0.0 13 0.12701204171857064 0.0 0.0 0.0 0.0 14 0.22562956449524926 0.0 0.0 0.0 0.0 15 0.3288268483915879 0.0 0.0 0.0 0.0 16 0.4878972179477785 0.0 0.0 0.0 0.0 17 0.6014752937153465 0.0 0.0 0.0 0.0 18 0.7535233628880584 0.0 0.0 0.0 0.0 19 0.9202266676436823 0.0 0.0 0.0 0.0 20 1.096700129454581 0.0 0.0 0.0 0.0 21 1.2792799394250263 0.0 0.0 0.0 0.0 22 1.4472045138125595 0.0 0.0 0.0 0.0 23 1.603221709288977 0.0 0.0 0.0 0.0 24 1.7796951710998754 0.0 0.0 0.0 0.0 25 1.9537260936469554 0.0 0.0 0.0 0.0 26 2.175997166654454 0.0 0.0 0.0 0.0 27 2.3698737207200606 0.0 0.0 0.0 0.0 28 2.592144793727559 0.0 0.0 0.0 0.0 29 2.8534964949561563 0.0 0.0 0.0 0.0 30 3.1914828655870644 0.0 0.0 0.0 0.0 31 3.490999242812828 0.0 0.0 0.0 0.0 32 3.710827776556508 0.0 0.0 0.0 0.0 33 3.8952394909748174 0.0 0.0 0.0 0.0 34 4.05858430424269 0.0 0.0 0.0 0.0 35 4.22467697418236 0.0 0.0 0.0 0.0 36 4.401455753401236 0.0 0.0 0.0 0.0 37 4.5861727852275225 0.0 0.0 0.0 0.0 38 4.775469578173469 0.0 0.0 0.0 0.0 39 4.978810971886373 0.0 0.0 0.0 0.0 40 5.187342761534891 0.0 0.0 0.0 0.0 41 5.398317090447229 0.0 0.0 0.0 0.0 42 5.603795706015974 0.0 0.0 0.0 0.0 43 5.815991304560221 0.0 0.0 0.0 0.0 44 6.010173176033804 0.0 0.0 0.0 0.0 45 6.199469968979751 0.0 0.0 0.0 0.0 46 6.379912557094356 0.0 0.0 0.0 0.0 47 6.566156175960528 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTTTA 135 0.0 53.000004 28 GGATGCT 135 0.0 53.000004 17 TTCACCG 75 0.0 53.000004 19 AAGTACT 75 0.0 53.000004 53 GGCATTT 150 0.0 53.000004 31 ATCTCTG 75 0.0 53.000004 26 TAAATTC 75 0.0 53.000004 47 TTCGCTA 150 0.0 53.000004 37 GAAACCT 75 0.0 53.000004 14 TTCAATT 150 0.0 53.000004 47 TTTCTTG 150 0.0 53.000004 50 TCCTAGA 75 0.0 53.000004 38 TAAGTTC 75 0.0 53.000004 33 CTGGGAT 135 0.0 53.000004 22 TATGTAC 75 0.0 53.000004 29 ATGGTTT 135 0.0 53.000004 27 ATGGTCT 75 0.0 53.000004 37 AATTAAT 150 0.0 53.000004 50 TTTCACC 75 0.0 53.000004 18 CAATTAA 150 0.0 53.000004 49 >>END_MODULE