FastQCFastQC Report
Fri 17 Jun 2016
SRR1528119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482769
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCTGTGTGGGGATGGCCAACATGGTGAAACCCCATCTCTACTAAAAATA25710.5325528358283155No Hit
AGATCTGTGTGGGGCTTCCGCTGGTGGCCCAGGGCTGTGCTTGGTTCTGTCA24860.5149460715166053No Hit
CTTGCTGTGTGGGGGCTGGCAAGATGGCTCAGCAGGCAAGAGCACTGACTGC24700.5116318570579305No Hit
CAGGCTGTGTGGGAAGCAGTGGTATCAGGGAGAAGAGGTCACAGGGAGACTC24000.4971321688012279No Hit
CAGGCTGTGTGGGAAGCAGTGGTATCAGGGAGGAGAGGTCACAGGGAGACTC22630.4687542074988245No Hit
ATAACTGTGTGGGGATCTTGTACTGAGCGGCAGCTAAAAGTGTTCTACCCAG22490.465854269847484No Hit
TCCACTGTGTGGGCTGCTTAGCGGTGCGGATCGCAGCTACGCGTGGGTGGTT21930.454254519242122No Hit
CGGCCTGTGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG21650.44845464393944107No Hit
GTAGCTGTGTGGGATCACTATCTGCCCGTGCCCATGGATGAGATGGGGGGGA20540.4254622811323842No Hit
GGATCTGTGTGGGGGAGCTGGGCCTGGTGGCACAAGCCAAGAATCAACTACT19310.39998425748132127No Hit
GATACTGTGTGGGGCTGGGTCTTTGTTGTTCCGAGGGCTTTCTCTAGTTGTG19310.39998425748132127No Hit
AGGTCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG18570.3846560156099501No Hit
ACTGCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTTTT18380.38072038594027374No Hit
TAACCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTCCCC18340.37989183232560503No Hit
AGGACTGTGTGGGGATATTTTTGGTCATGAATTTATGGGGGAAGTCGTTGAA17970.3722277113899194No Hit
TTAACTGTGTGGGGAGGAGAGGCAGTAGAGGCAGTAGAGCTGCGGAGCCTCA17460.3616636528028933No Hit
TTAACTGTGTGGGGCCGGTTCTGGTGGCGCAGGCCGGTAGGATTTGCCTCCC17360.3595922687662215No Hit
GCGTCTGTGTGGGGAAGGATTCTAGCTAAAAAATGCAGAAGGAGGCCAGGCG15980.33100716906015093No Hit
GCATCTGTGTGGGCATTCGCCTCAGCACATGATTATTCCCAAGGATAAACCA15440.3198216952621233No Hit
GTTACTGTGTGGGAGGCCGAGGTGGGAGGGTCACAAGGTCAAGAGATTGAGA14810.30677197583109106No Hit
GGTGCTGTGTGGGGTAGCCAACATGCAGCTCTTTGTCCGCGCCCAGGAACTA14350.29724360926240084No Hit
GGTTCTGTGTGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTGC14240.2949650868220619No Hit
CTTACTGTGTGGGTTCCCTTTTTATCATTGGCAAGCCTTGGAATCTATCATA14200.2941365332073932No Hit
TGCTCTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC14100.2920651491707214No Hit
TTCCCTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGC13840.28667955067537476No Hit
TTGACTGTGTGGGACATGATCATCTCCTTAGATGCTGAAAAAGCATTTGACA13360.2767369072993502No Hit
GCTGCTGTGTGGGGCTCAGATGCTCATTGCCCTGTCAGCTGTCGAGAGCCGA13350.27652976889568304No Hit
GCTGCTGTGTGGTGACCCGCTTTTGCCAGCCTTGAAGGCCTGCGGCTACCGG13300.27549407687734717No Hit
AAGGCTGTGTGGGGCTTCCTTTCCTTTCAGCGGCGCGCGGCTGCAAGATGGC13200.27342269284067533No Hit
TGGCCTGTGTGGGGACATGGCGCCCTCTTTCTATCACTATCTGCCCGTGCCC13090.2711441704003364No Hit
TGCCCTGTGTGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC13070.27072989359300204No Hit
TGCGCTGTGTGGGGTGCTGCTGTGTGGGTACACTCACGTTCCTTTGTCTCCA12930.26782995594166153No Hit
TTCACTGTGTGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCA12930.26782995594166153No Hit
TTGACTGTGTGGGTGGTATCCCGTGGCCCAGTCAACACATAAAATTAACCAT12570.2603729734096431No Hit
TGAGCTGTGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCT12390.25664448214363395No Hit
ACTGCTGTGTGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGG11980.2481518075932796No Hit
GGTACTGTGTGGGGGAGCTGGGCCTGGTGGCACAAGCCAAGAATCAACTACT11930.24711611557494373No Hit
TTTTCTGTGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTC11480.2377948874099207No Hit
GGTACTGTGTGGGGGAACCTCCAAAGTATGGCAGAAGCGAGGTTGACTTGGT11350.23510208816224734No Hit
GCGTCTGTGTGGGGTGAGGCCTCATCTCCACAAAAAATCAAAAAAGTATCTG11250.23303070412557558No Hit
AGGGCTGTGTGGGTATTCGGCTACAATTTTTCCTCAGTTAAGCATAATTTCA11250.23303070412557558No Hit
GTCCCTGTGTGGGGACCTTCAAGGCTCCACCATGTTCTCGCGGGCGAGCGTT11040.22868079764856486No Hit
GTAGCTGTGTGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGT10800.22370947596055255No Hit
TGAGCTGTGTGGGGGCACCAACTCAGAGTCTTGTTGGAGCTTTATCCCTTTG10660.22080953830921207No Hit
GCGTCTGTGTGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCACTGACTG10570.2189452926762075No Hit
TGATCTGTGTGGGGACTGGAGCGATGGCTCAACAGCCAAGAGCACTGACTGC10390.21521680141019825No Hit
TCTACTGTGTGGGGACCGCCTCAGCTGTTCGCGTTTCGCCATGTCGGGGTCC10280.21293827896985928No Hit
TTACCTGTGTGGGGCAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCG10110.20941692610751728No Hit
CGTACTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG10090.20900264930018292No Hit
TTGTCTGTGTGGGACTTGAACTATTTCTTGTCTAAATTCTTGCACAATGAGT9910.20527415803417368No Hit
CATTCTGTGTGGGAGTGGAGAAGGAACATGAGGCCACCTTATGTGACCATCT9590.198645729116824No Hit
GTAACTGTGTGGGTCTCCCAGTATTATTCTGCCAGCATACCAGAAGTCTGGT9570.19823145230948963No Hit
ATATCTGTGTGGGACACCAGGTCCCACCAGCCCCTGGACTGTGGTGTCCAGT9530.1974028986948209No Hit
ACTGCTGTGTGGGAGAACATTCAGGTATTTGATTTCACCTTTAGCCCAGAGG9500.19678148348381938No Hit
TTTGCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACT9310.192845853814143No Hit
CAATCTGTGTGGGGGATATAAAAGTTTGTCGCCGCTGTCTTGTTAGGGGGAC9260.1918101617958071No Hit
TGTACTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG9220.1909816081811384No Hit
GTGACTGTGTGGGAGGCTGGAGAGATGGCTCAGTGGTTATGAGCGCTGACTG8900.1843531792637887No Hit
TTCACTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG8710.1804175495941123No Hit
CGTTCTGTGTGGGAAGCAATGGTATCAACGCAGAATCTAGCTGTGTGGGGGG8690.18000327278677794No Hit
GCCACTGTGTGGGGGGAGTCTGGAAAGTTAGAAAGTCTGGGAGGGCGGCAGC8630.17876044236477487No Hit
TTAACTGTGTGGGAGGTGGCTTTATGAATGAATTCTACCAAACATTTAAAGA8500.17606764311710157No Hit
AATACTGTGTGGGCGCTCCCAGGGGGGCAGCAGTCTGCAGGATGGCTCGTTG8460.17523908950243283No Hit
CATCCTGTGTGGGTAACTGATGAGGGCTAAGTAAATTTGAATGAAATTTTCT8430.1746176742914313No Hit
GCTACTGTGTGGGGGCAAGGTTCTAAGTTAATCTTTTTTTGTGAATTGCTTT8430.1746176742914313No Hit
TTTCCTGTGTGGGTCCCATCCTGATCACCATCATAGCCATGAGTGCCCTGGG8410.17420339748409694No Hit
TGTGCTGTGTGGGGCCATATAAAGTCTATAGCCTATTTCTGTACCTGTTGTG8360.17316770546576105No Hit
AGGACTGTGTGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCA8250.1708891830254221No Hit
GGCCCTGTGTGGGAGAAGAGTAGAAGGAAGACTAACAGAATCAACTAACCTG8200.1698534910070862No Hit
ATTACTGTGTGGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCG8180.16943921419975183No Hit
GTTTCTGTGTGGGCTGTGTGGGTAGCTACCTAACATTTTCCCGGCAGGAAAA8140.16861066058508312No Hit
CGAACTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAG8130.16840352218141597No Hit
CTACCTGTGTGGGGTAAACTAGGTGCTGCCAAGATGGCTCAGCGGTTAAGAG8070.1671606917594129No Hit
CTTACTGTGTGGGCGGGGCTTCAGGCCTGCCTCCGAGGAGATCAAGACCCTC7930.1642607541080724No Hit
ATCGCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACT7890.16343220049340368No Hit
AGCACTGTGTGGGGGTCGCTAGGTTTGTTGAAGGACATCGGCCGCCGAGTTT7890.16343220049340368No Hit
GATTCTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC7720.15991084763106164No Hit
GGGACTGTGTGGGATTCTACTGCTGAGCTGTGCCTAGCCACGGAGACTTCCT7690.1592894324200601No Hit
TTAGCTGTGTGGGTTCTGCTGAGTCAAACATCTGGTATGGGCAGTGTCATCT7660.15866801720905857No Hit
CGTGCTGTGTGGGCAGTGACAATGAGGAGGAGCTGGTAGAGAGACTTGAGAG7630.15804660199805703No Hit
GCCGCTGTGTGGGATCCAGGACACATGGTGTGCCACGCTTATCCAGCCTCCA7510.1555609411540509No Hit
GCTTCTGTGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCC7480.15493952594304936No Hit
GTAACTGTGTGGGAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACC7410.1534895571173791No Hit
TCCGCTGTGTGGGCAGAGTTGTGCTGTGTGGGATATTGACCGATTGTCAGAG7340.15203958829170888No Hit
GGTACTGTGTGGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTG7330.1518324498880417No Hit
GCTACTGTGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGG7300.15121103467704017No Hit
GAATCTGTGTGGGGCTTTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGG7220.14955392744770274No Hit
TATGCTGTGTGGGAGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGAC6990.14478974416335763No Hit
TTAGCTGTGTGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACAT6860.1420969449156843No Hit
AGTCCTGTGTGGGGAGTCTGTGCAATGCTTTAATGCAGCAAGGAACAAAGAA6810.14106125289734842No Hit
TGAACTGTGTGGGGAGGCCAGGCCGGGCGGCGGCTCACCAGGACGAACCATG6800.14085411449368124No Hit
TGAGCTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA6750.13981842247534534No Hit
GGCTCTGTGTGGGAGCTTTTGCATAGCACTCTTTTTTTTTTAGATTTATTTA6690.13857559205334227No Hit
GGCGCTGTGTGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACATGG6630.13733276163133923No Hit
ATTGCTGTGTGGGCAGGGTTCTCTAAGTGAACAGCATTGGTAGAATGAATAT6630.13733276163133923No Hit
AGTTCTGTGTGGGAGACTGTCTTTGCTTCATCATCTGAAGGTAAAATTTTCC6620.13712562322767205No Hit
GCTGCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG6520.13505423919100024No Hit
AGTCCTGTGTGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAG6450.13360427036533No Hit
GATACTGTGTGGGACCTTGCTGCCTCTTAAAAACTGTGTGTGTGTGTGTGAT6440.13339713196166283No Hit
AACTCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG6380.13215430153965976No Hit
AGACCTGTGTGGGAACCTGTTAGGCTATCTCTAATGACTATAGAGGGAGCAC6350.13153288632865823No Hit
TCACCTGTGTGGGAGAGGTGGCATGCAGATCTTTGTGAAGACCCTGACAGGC6350.13153288632865823No Hit
TGTGCTGTGTGGGCAAGCAGCTCTACCAGGTTCTCACAGTAAATATTGGGGG6320.13091147111765666No Hit
TCATCTGTGTGGGGGGCGCAGGACCCTAGGGCTCCTCCCGTGCTCCTCTCCG6280.13008291750298798No Hit
TGGACTGTGTGGGGAGGTCTCCAGTGCCGAGCACAGGCACCTGCCATCAGTA6240.12925436388831926No Hit
ATACCTGTGTGGGGAAGCCTCACCGCTACAGGCCAGGGACCGTGGCTCTGAG6160.12759725665898183No Hit
TCACCTGTGTGGGGTACCTGTGTATTTATCCTTGATCATAGTTTGCTTATTT6160.12759725665898183No Hit
TAGTCTGTGTGGGGGTAAAGGCGCTTGCCACCAAGCCTGGAGACCTGAGTTT6110.12656156464064594No Hit
CGTTCTGTGTGGGGGTGCTGGCCATCCTCAGCACACTTTTAATTGGACTGCT6110.12656156464064594No Hit
CAAACTGTGTGGGACGAAACCAGTTTTCTTTGCCGACAATAACTGGCACCAC6050.12531873421864287No Hit
TTTGCTGTGTGGGGGAGACAGGCCTGGGGTTAGATCATCAGTGCTGCAAGAA5880.12179738135630085No Hit
CTAGCTGTGTGGGGCATTCAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTC5810.12034741253063058No Hit
ATGGCTGTGTTCCTCATTCTGTGCTCTACTCAGGAGGTATTTTGACATGAAA5760.11931172051229472No Hit
TGATCTGTGTGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGA5640.11682605966828856No Hit
GCGTCTGTGTGGGATGAGCAAAGATGAGAGTGGTTGGTTTGGTAAAGAACTT5620.1164117828609542No Hit
AAGGCTGTGTGGGAGTCAGTTAGGTGACTCTTAGCATGCGCAGGCCAGCTGA5610.11620464445728702No Hit
ATTTCTGTGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGC5590.11579036764995265No Hit
GTTGCTGTGTGGGGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCT5580.11558322924628547No Hit
AAGCCTGTGTGGGAAGTAGGGTAATGCCATTTGTAGATAGGTCATTGATACA5510.11413326042061525No Hit
TTGGCTGTGTGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG5490.11371898361328088No Hit
AATTCTGTGTGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC5490.11371898361328088No Hit
TACCCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG5480.1135118452096137No Hit
GGTGCTGTGTGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCA5220.10812624671426706No Hit
GTGTCTGTGTGGGGAAGGTTCACGTTGGAGTGCTTCGCGACTGCGTCGGGGA5210.1079191083105999No Hit
CCTACTGTGTGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG5170.10709055469593119No Hit
TATTCTGTGTGGGATTGCTTAGGCACTGAAAGTCCTTATCCCTGGCTTAGCC5150.10667627788859682No Hit
GGAACTGTGTGGGGAGTCCATCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA5150.10667627788859682No Hit
GTATCTGTGTGGGGAGTGCCATCTGGGCACTGTGCGCTTCCTGCACAACTCC5140.10646913948492964No Hit
CGAACTGTGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5100.10564058587026093No Hit
GAGACTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA5100.10564058587026093No Hit
TGATCTGTGTGGGGCTCTTTTACCGAGACCCGCCAACATGGGCCGCGTTCGC5100.10564058587026093No Hit
GGCTCTGTGTGGGCATTTTATTTATTATAGTTTGTGTGTATAGATGTTTTGC5070.10501917065925939No Hit
GTCTCTGTGTGGGACCATGTCATTAGCCAAGCATGAGGGGCAGTTTCAACCC5040.10439775544825786No Hit
TGATCTGTGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATAC4870.10087640258591582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTA700.046.00000417
GCCCTAG700.046.00000424
GCCCTAC700.046.00000430
CGCGACT700.046.00000436
CGCTGGT2800.046.00000419
TAGAAGA351.01792466E-746.00000446
CTACACA700.046.00000433
GAGGCGA351.01792466E-746.00000413
CTCTGAG700.046.00000446
ATCCTAG700.046.00000418
TCCTTTG1650.046.00000440
ACTAATC700.046.00000414
TTGTTGT1400.046.00000423
GGAGACC700.046.00000439
ACTGCAG351.01792466E-746.00000444
AATCCTA700.046.00000417
TTCACGT700.046.00000419
TTCATCG351.01792466E-746.00000419
AAACTAG700.046.00000416
TCAAGTA351.01792466E-746.00000422
Produced by FastQC (version 0.11.5)