FastQCFastQC Report
Fri 17 Jun 2016
SRR1528118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477710
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCTGTGTGGGGATGGCCAACATGGTGAAACCCCATCTCTACTAAAAATA24630.5155847690021143No Hit
AGATCTGTGTGGGGCTTCCGCTGGTGGCCCAGGGCTGTGCTTGGTTCTGTCA24280.5082581482489377No Hit
CTTGCTGTGTGGGGGCTGGCAAGATGGCTCAGCAGGCAAGAGCACTGACTGC24190.5063741600552636No Hit
CAGGCTGTGTGGGAAGCAGTGGTATCAGGGAGAAGAGGTCACAGGGAGACTC23250.48669695003244645No Hit
CAGGCTGTGTGGGAAGCAGTGGTATCAGGGAGGAGAGGTCACAGGGAGACTC22050.46157710745012664No Hit
CGGCCTGTGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG21220.44420254966402206No Hit
ATAACTGTGTGGGGATCTTGTACTGAGCGGCAGCTAAAAGTGTTCTACCCAG21190.4435745535994641No Hit
TCCACTGTGTGGGCTGCTTAGCGGTGCGGATCGCAGCTACGCGTGGGTGGTT21040.4404345732766741No Hit
GTAGCTGTGTGGGATCACTATCTGCCCGTGCCCATGGATGAGATGGGGGGGA19770.413849406543719No Hit
TAACCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTCCCC19560.409453434091813No Hit
GATACTGTGTGGGGCTGGGTCTTTGTTGTTCCGAGGGCTTTCTCTAGTTGTG19100.39982416110192376No Hit
GGATCTGTGTGGGGGAGCTGGGCCTGGTGGCACAAGCCAAGAATCAACTACT19060.3989868330158465No Hit
ACTGCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTTTT18290.38286826735885787No Hit
AGGACTGTGTGGGGATATTTTTGGTCATGAATTTATGGGGGAAGTCGTTGAA17730.3711456741537753No Hit
AGGTCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG17150.35900441690565404No Hit
TTAACTGTGTGGGGCCGGTTCTGGTGGCGCAGGCCGGTAGGATTTGCCTCCC16910.3539804483891901No Hit
TTAACTGTGTGGGGAGGAGAGGCAGTAGAGGCAGTAGAGCTGCGGAGCCTCA15940.33367524230181494No Hit
GCGTCTGTGTGGGGAAGGATTCTAGCTAAAAAATGCAGAAGGAGGCCAGGCG15750.3296979338929476No Hit
GCATCTGTGTGGGCATTCGCCTCAGCACATGATTATTCCCAAGGATAAACCA15440.32320864122584836No Hit
GGTGCTGTGTGGGGTAGCCAACATGCAGCTCTTTGTCCGCGCCCAGGAACTA14210.2974608025789705No Hit
AAGGCTGTGTGGGGCTTCCTTTCCTTTCAGCGGCGCGCGGCTGCAAGATGGC14110.2953674823637772No Hit
GGTTCTGTGTGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTGC14060.29432082225618056No Hit
GTTACTGTGTGGGAGGCCGAGGTGGGAGGGTCACAAGGTCAAGAGATTGAGA13970.29243683406250653No Hit
TTCCCTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGC13860.29013418182579387No Hit
TGCGCTGTGTGGGGTGCTGCTGTGTGGGTACACTCACGTTCCTTTGTCTCCA13600.2846915492662913No Hit
CTTACTGTGTGGGTTCCCTTTTTATCATTGGCAAGCCTTGGAATCTATCATA13480.2821795650080593No Hit
GCTGCTGTGTGGTGACCCGCTTTTGCCAGCCTTGAAGGCCTGCGGCTACCGG13320.27883025266374994No Hit
TTCACTGTGTGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCA13230.276946264470076No Hit
TGCTCTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC13190.2761089363839987No Hit
GCTGCTGTGTGGGGCTCAGATGCTCATTGCCCTGTCAGCTGTCGAGAGCCGA13150.27527160829792136No Hit
TGGCCTGTGTGGGGACATGGCGCCCTCTTTCTATCACTATCTGCCCGTGCCC13020.27255029201817005No Hit
TTGACTGTGTGGGACATGATCATCTCCTTAGATGCTGAAAAAGCATTTGACA12650.26480500722195477No Hit
TTGACTGTGTGGGTGGTATCCCGTGGCCCAGTCAACACATAAAATTAACCAT12540.26250235498524205No Hit
TGCCCTGTGTGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC12430.26019970274852944No Hit
AGGGCTGTGTGGGTATTCGGCTACAATTTTTCCTCAGTTAAGCATAATTTCA11990.25098909380167883No Hit
GGTACTGTGTGGGGGAGCTGGGCCTGGTGGCACAAGCCAAGAATCAACTACT11650.24387180507002157No Hit
TGAGCTGTGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCT11430.23926650059659627No Hit
TGATCTGTGTGGGGACTGGAGCGATGGCTCAACAGCCAAGAGCACTGACTGC11330.23717318038140295No Hit
GCGTCTGTGTGGGGTGAGGCCTCATCTCCACAAAAAATCAAAAAAGTATCTG11210.23466119612317093No Hit
ACTGCTGTGTGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGG11040.23110255175734234No Hit
TCTACTGTGTGGGGACCGCCTCAGCTGTTCGCGTTTCGCCATGTCGGGGTCC11020.23068388771430365No Hit
TTTTCTGTGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTC10980.2298465596282263No Hit
GGTACTGTGTGGGGGAACCTCCAAAGTATGGCAGAAGCGAGGTTGACTTGGT10830.22670657930543633No Hit
GTAGCTGTGTGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGT10800.22607858324087834No Hit
GTCCCTGTGTGGGGACCTTCAAGGCTCCACCATGTTCTCGCGGGCGAGCGTT10580.22147327876745307No Hit
TTACCTGTGTGGGGCAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCG10310.2158213141864311No Hit
CGTACTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG10140.21226266982060246No Hit
GCGTCTGTGTGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCACTGACTG9980.20891335747629317No Hit
TGAGCTGTGTGGGGGCACCAACTCAGAGTCTTGTTGGAGCTTTATCCCTTTG9940.20807602939021583No Hit
CAATCTGTGTGGGGGATATAAAAGTTTGTCGCCGCTGTCTTGTTAGGGGGAC9560.2001214125724812No Hit
ACTGCTGTGTGGGAGAACATTCAGGTATTTGATTTCACCTTTAGCCCAGAGG9440.19760942831424924No Hit
TGTACTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG9420.19719076427121054No Hit
CATTCTGTGTGGGAGTGGAGAAGGAACATGAGGCCACCTTATGTGACCATCT9390.19656276820665258No Hit
TTTGCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACT9380.19635343618513326No Hit
TTGTCTGTGTGGGACTTGAACTATTTCTTGTCTAAATTCTTGCACAATGAGT9290.19446944799145924No Hit
GTAACTGTGTGGGTCTCCCAGTATTATTCTGCCAGCATACCAGAAGTCTGGT9090.1902828075610726No Hit
CATCCTGTGTGGGTAACTGATGAGGGCTAAGTAAATTTGAATGAAATTTTCT8880.18588683510916665No Hit
ATATCTGTGTGGGACACCAGGTCCCACCAGCCCCTGGACTGTGGTGTCCAGT8850.18525883904460866No Hit
GTGACTGTGTGGGAGGCTGGAGAGATGGCTCAGTGGTTATGAGCGCTGACTG8600.18002553850662537No Hit
GCCACTGTGTGGGGGGAGTCTGGAAAGTTAGAAAGTCTGGGAGGGCGGCAGC8460.1770948902053547No Hit
TTCACTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG8420.17625756211927737No Hit
TTAACTGTGTGGGAGGTGGCTTTATGAATGAATTCTACCAAACATTTAAAGA8390.17562956605471938No Hit
ATCGCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACT8370.1752109020116807No Hit
GCTACTGTGTGGGGGCAAGGTTCTAAGTTAATCTTTTTTTGTGAATTGCTTT8320.17416424190408408No Hit
GGCCCTGTGTGGGAGAAGAGTAGAAGGAAGACTAACAGAATCAACTAACCTG8260.17290824977496808No Hit
CGAACTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAG8230.1722802537104101No Hit
ATTACTGTGTGGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCG8220.17207092168889074No Hit
CTACCTGTGTGGGGTAAACTAGGTGCTGCCAAGATGGCTCAGCGGTTAAGAG8120.16997760147369742No Hit
CGTTCTGTGTGGGAAGCAATGGTATCAACGCAGAATCTAGCTGTGTGGGGGG8110.1697682694521781No Hit
TGTGCTGTGTGGGGCCATATAAAGTCTATAGCCTATTTCTGTACCTGTTGTG8040.16830294530154277No Hit
GGTACTGTGTGGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTG7970.16683762115090747No Hit
CGTGCTGTGTGGGCAGTGACAATGAGGAGGAGCTGGTAGAGAGACTTGAGAG7820.16369764082811747No Hit
AATACTGTGTGGGCGCTCCCAGGGGGGCAGCAGTCTGCAGGATGGCTCGTTG7810.16348830880659815No Hit
GTTTCTGTGTGGGCTGTGTGGGTAGCTACCTAACATTTTCCCGGCAGGAAAA7770.1626509807205208No Hit
TTAGCTGTGTGGGTTCTGCTGAGTCAAACATCTGGTATGGGCAGTGTCATCT7700.1611856565698855No Hit
AGCACTGTGTGGGGGTCGCTAGGTTTGTTGAAGGACATCGGCCGCCGAGTTT7620.15951100039773083No Hit
TTTCCTGTGTGGGTCCCATCCTGATCACCATCATAGCCATGAGTGCCCTGGG7580.15867367231165352No Hit
TGAGCTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA7570.15846434029013418No Hit
GGGACTGTGTGGGATTCTACTGCTGAGCTGTGCCTAGCCACGGAGACTTCCT7560.15825500826861486No Hit
GCTTCTGTGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCC7530.15762701220405684No Hit
AGGACTGTGTGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCA7400.15490569592430556No Hit
CTTACTGTGTGGGCGGGGCTTCAGGCCTGCCTCCGAGGAGATCAAGACCCTC7370.15427769985974754No Hit
AGACCTGTGTGGGAACCTGTTAGGCTATCTCTAATGACTATAGAGGGAGCAC7280.15239371166607354No Hit
GCTACTGTGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGG7280.15239371166607354No Hit
TCCGCTGTGTGGGCAGAGTTGTGCTGTGTGGGATATTGACCGATTGTCAGAG7220.15113771953695757No Hit
GTAACTGTGTGGGAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACC7170.1500910594293609No Hit
GAATCTGTGTGGGGCTTTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGG7160.14988172740784156No Hit
TGAACTGTGTGGGGAGGCCAGGCCGGGCGGCGGCTCACCAGGACGAACCATG7070.1479977392141676No Hit
GCCGCTGTGTGGGATCCAGGACACATGGTGTGCCACGCTTATCCAGCCTCCA6970.14590441899897427No Hit
GGCTCTGTGTGGGAGCTTTTGCATAGCACTCTTTTTTTTTTAGATTTATTTA6950.1454857549559356No Hit
GATTCTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC6920.1448577588913776No Hit
GGCGCTGTGTGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACATGG6580.13774047015972032No Hit
TATGCTGTGTGGGAGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGAC6570.137531138138201No Hit
TCACCTGTGTGGGAGAGGTGGCATGCAGATCTTTGTGAAGACCCTGACAGGC6530.13669381005212367No Hit
AACTCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG6460.13522848590148837No Hit
GATACTGTGTGGGACCTTGCTGCCTCTTAAAAACTGTGTGTGTGTGTGTGAT6380.1335538297293337No Hit
AGTCCTGTGTGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAG6370.13334449770781437No Hit
AGTTCTGTGTGGGAGACTGTCTTTGCTTCATCATCTGAAGGTAAAATTTTCC6330.13250716962173703No Hit
TGGACTGTGTGGGGAGGTCTCCAGTGCCGAGCACAGGCACCTGCCATCAGTA6320.1322978376002177No Hit
TTTGCTGTGTGGGGGAGACAGGCCTGGGGTTAGATCATCAGTGCTGCAAGAA6280.13146050951414037No Hit
AGTCCTGTGTGGGGAGTCTGTGCAATGCTTTAATGCAGCAAGGAACAAAGAA6280.13146050951414037No Hit
TTAGCTGTGTGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACAT6240.13062318142806306No Hit
ATACCTGTGTGGGGAAGCCTCACCGCTACAGGCCAGGGACCGTGGCTCTGAG6220.1302045173850244No Hit
GCTGCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG6130.1283205291913504No Hit
TAGTCTGTGTGGGGGTAAAGGCGCTTGCCACCAAGCCTGGAGACCTGAGTTT6010.1258085449331184No Hit
TCACCTGTGTGGGGTACCTGTGTATTTATCCTTGATCATAGTTTGCTTATTT5970.12497121684704109No Hit
TGATCTGTGTGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGA5860.12266856461032845No Hit
TCATCTGTGTGGGGGGCGCAGGACCCTAGGGCTCCTCCCGTGCTCCTCTCCG5850.12245923258880911No Hit
CAAACTGTGTGGGACGAAACCAGTTTTCTTTGCCGACAATAACTGGCACCAC5780.12099390843817379No Hit
ATTGCTGTGTGGGCAGGGTTCTCTAAGTGAACAGCATTGGTAGAATGAATAT5700.11931925226601914No Hit
TGTGCTGTGTGGGCAAGCAGCTCTACCAGGTTCTCACAGTAAATATTGGGGG5670.11869125620146113No Hit
GGAACTGTGTGGGGAGTCCATCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA5620.11764459609386448No Hit
CTAGCTGTGTGGGGCATTCAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTC5620.11764459609386448No Hit
CGTTCTGTGTGGGGGTGCTGGCCATCCTCAGCACACTTTTAATTGGACTGCT5610.11743526407234516No Hit
GGTGCTGTGTGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCA5480.11471394779259383No Hit
ATTTCTGTGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGC5470.11450461577107451No Hit
GTTGCTGTGTGGGGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCT5440.1138766197065165No Hit
AATTCTGTGTGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC5370.11241129555588118No Hit
AAGGCTGTGTGGGAGTCAGTTAGGTGACTCTTAGCATGCGCAGGCCAGCTGA5340.1117832994913232No Hit
GCGTCTGTGTGGGATGAGCAAAGATGAGAGTGGTTGGTTTGGTAAAGAACTT5340.1117832994913232No Hit
CCTACTGTGTGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG5310.1111553034267652No Hit
GTCTCTGTGTGGGACCATGTCATTAGCCAAGCATGAGGGGCAGTTTCAACCC5300.11094597140524586No Hit
AAGCCTGTGTGGGAAGTAGGGTAATGCCATTTGTAGATAGGTCATTGATACA5250.1098993112976492No Hit
TACCCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG5220.10927131523309122No Hit
TTGGCTGTGTGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG5210.10906198321157187No Hit
ATGGCTGTGTTCCTCATTCTGTGCTCTACTCAGGAGGTATTTTGACATGAAA5140.10759665906093654No Hit
TGATCTGTGTGGGGCTCTTTTACCGAGACCCGCCAACATGGGCCGCGTTCGC4970.10403801469510791No Hit
GTATCTGTGTGGGGAGTGCCATCTGGGCACTGTGCGCTTCCTGCACAACTCC4940.10341001863054991No Hit
GGTGCTGTGTGGGAGCTTAAGGTTTTAAACTTAGAAAGACACTAGAGTGCCT4930.10320068660903059No Hit
TATTCTGTGTGGGATTGCTTAGGCACTGAAAGTCCTTATCCCTGGCTTAGCC4810.1006887023507986No Hit
GAGACTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA4810.1006887023507986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTT1450.046.00000424
TGGTTTG351.0178883E-746.00000435
CTACAAT1450.046.00000421
TCCTCTA351.0178883E-746.00000424
GTTTGTT700.046.00000423
TTGTTGT1450.046.00000423
TTGTTGA700.046.00000425
TTGTTCC1450.046.00000426
ACGCCTC1450.046.00000427
TGCATGG351.0178883E-746.00000424
TGGCAGC351.0178883E-746.00000444
ACTAAAG351.0178883E-746.00000438
GTGCATG351.0178883E-746.00000423
GTGTTCA351.0178883E-746.00000413
CTATAGC700.046.00000426
CAGTCAA1450.046.00000429
CAGTTAG351.0178883E-746.00000417
CAGTTAA1450.046.00000435
TAAATGA351.0178883E-746.00000438
GTCACAG4650.046.00000438
Produced by FastQC (version 0.11.5)