FastQCFastQC Report
Fri 17 Jun 2016
SRR1528117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440569
Sequences flagged as poor quality0
Sequence length59
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCTGTGTGGGGATGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAAT21750.4936797641232134No Hit
CAGGCTGTGTGGGAAGCAGTGGTATCAGGGAGAAGAGGTCACAGGGAGACTCATCAACC21020.4771102823848251No Hit
CAGGCTGTGTGGGAAGCAGTGGTATCAGGGAGGAGAGGTCACAGGGAGACTCATCAACC20950.475521427971555No Hit
AGATCTGTGTGGGGCTTCCGCTGGTGGCCCAGGGCTGTGCTTGGTTCTGTCAGCCGGCT19940.4525965285800862No Hit
CTTGCTGTGTGGGGGCTGGCAAGATGGCTCAGCAGGCAAGAGCACTGACTGCTCTTCTG19510.44283642289856984No Hit
ATAACTGTGTGGGGATCTTGTACTGAGCGGCAGCTAAAAGTGTTCTACCCAGTGCCCTG19090.4333032964189491No Hit
TCCACTGTGTGGGCTGCTTAGCGGTGCGGATCGCAGCTACGCGTGGGTGGTTTACCCGG17500.39721360331752803No Hit
CGGCCTGTGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGC17310.3929009984815091No Hit
GATACTGTGTGGGGCTGGGTCTTTGTTGTTCCGAGGGCTTTCTCTAGTTGTGGTGCACC16700.3790552671658696No Hit
GTAGCTGTGTGGGATCACTATCTGCCCGTGCCCATGGATGAGATGGGGGGGAAGCAAGG16370.3715649535033105No Hit
GGATCTGTGTGGGGGAGCTGGGCCTGGTGGCACAAGCCAAGAATCAACTACTCCAGGGA16130.366117452657813No Hit
TTAACTGTGTGGGGAGGAGAGGCAGTAGAGGCAGTAGAGCTGCGGAGCCTCAGGCTTCT16060.36452859824454287No Hit
GCATCTGTGTGGGCATTCGCCTCAGCACATGATTATTCCCAAGGATAAACCATATGTTA16040.3640746398407514No Hit
ACTGCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTTTTTGGTCTA15490.3515907837364862No Hit
TAACCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTCCCCGAGTGCT15080.3422846364587613No Hit
AGGTCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG14950.3393339068341168No Hit
AGGACTGTGTGGGGATATTTTTGGTCATGAATTTATGGGGGAAGTCGTTGAAACCGGAA14940.33910692763222106No Hit
TTAACTGTGTGGGGCCGGTTCTGGTGGCGCAGGCCGGTAGGATTTGCCTCCCAAGTACT13410.3043791097421743No Hit
GCGTCTGTGTGGGGAAGGATTCTAGCTAAAAAATGCAGAAGGAGGCCAGGCGCGGTGGC12270.27850348072606107No Hit
GGTTCTGTGTGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCG11860.26919733344833613No Hit
GTTACTGTGTGGGAGGCCGAGGTGGGAGGGTCACAAGGTCAAGAGATTGAGACCATCCT11760.2669275414293788No Hit
GGTGCTGTGTGGGGTAGCCAACATGCAGCTCTTTGTCCGCGCCCAGGAACTACACACCC11700.2655656662180044No Hit
TGCGCTGTGTGGGGTGCTGCTGTGTGGGTACACTCACGTTCCTTTGTCTCCATCAACCT11400.25875629016113255No Hit
CTTACTGTGTGGGTTCCCTTTTTATCATTGGCAAGCCTTGGAATCTATCATATTTGACT11190.2539897269213222No Hit
TGCCCTGTGTGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG11180.2537627477194265No Hit
GCTGCTGTGTGGGGCTCAGATGCTCATTGCCCTGTCAGCTGTCGAGAGCCGAGAGCCAG10890.2471803508644503No Hit
TGCTCTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCA10870.24672639246065883No Hit
TTGACTGTGTGGGACATGATCATCTCCTTAGATGCTGAAAAAGCATTTGACAAAATAGA10770.24445660044170153No Hit
GCGTCTGTGTGGGGTGAGGCCTCATCTCCACAAAAAATCAAAAAAGTATCTGCGTGTGG10770.24445660044170153No Hit
AGGGCTGTGTGGGTATTCGGCTACAATTTTTCCTCAGTTAAGCATAATTTCACCAGAAA10450.23719326598103815No Hit
TGGCCTGTGTGGGGACATGGCGCCCTCTTTCTATCACTATCTGCCCGTGCCCATGGATG10350.23492347396208085No Hit
TTCACTGTGTGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA10230.23219972353933208No Hit
TTCCCTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGC10150.23038388992416622No Hit
TTGACTGTGTGGGTGGTATCCCGTGGCCCAGTCAACACATAAAATTAACCATCACAAGT9960.22607128508814736No Hit
AAGGCTGTGTGGGGCTTCCTTTCCTTTCAGCGGCGCGCGGCTGCAAGATGGCGGTGCAG9880.2242554514729815No Hit
GCTGCTGTGTGGTGACCCGCTTTTGCCAGCCTTGAAGGCCTGCGGCTACCGGACGGAAG9540.2165381586085267No Hit
TGATCTGTGTGGGGACTGGAGCGATGGCTCAACAGCCAAGAGCACTGACTGCTCTTCCA9530.21631117940663097No Hit
GCGTCTGTGTGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCACTGACTGCTCTTCC9270.210409720157342No Hit
GGTACTGTGTGGGGGAGCTGGGCCTGGTGGCACAAGCCAAGAATCAACTACTCCAGGGA8970.2036003441004701No Hit
TGAGCTGTGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTAC8950.2031463856966786No Hit
ACTGCTGTGTGGGAGAACATTCAGGTATTTGATTTCACCTTTAGCCCAGAGGAGATGAA8940.2029194064947829No Hit
TGTACTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGC8920.20246544809099143No Hit
TTACCTGTGTGGGGCAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC8880.2015575312834085No Hit
GTAGCTGTGTGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGTGCTTGTG8840.20064961447582558No Hit
TTTGCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC8300.18839273757345615No Hit
TTTTCTGTGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGA8270.18771179996776896No Hit
ACTGCTGTGTGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGTGTTGAC8230.18680388316018604No Hit
TTGTCTGTGTGGGACTTGAACTATTTCTTGTCTAAATTCTTGCACAATGAGTTCTATTA8220.18657690395829032No Hit
CATTCTGTGTGGGAGTGGAGAAGGAACATGAGGCCACCTTATGTGACCATCTCTCACAT8150.18498804954502018No Hit
TCTACTGTGTGGGGACCGCCTCAGCTGTTCGCGTTTCGCCATGTCGGGGTCCCCGGTAA8150.18498804954502018No Hit
GGTACTGTGTGGGGGAACCTCCAAAGTATGGCAGAAGCGAGGTTGACTTGGTGATAAAG8130.18453409114122873No Hit
TGAGCTGTGTGGGGGCACCAACTCAGAGTCTTGTTGGAGCTTTATCCCTTTGTCCTAGC7970.18090242391089706No Hit
CGAACTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCT7930.17999450710331413No Hit
CTACCTGTGTGGGGTAAACTAGGTGCTGCCAAGATGGCTCAGCGGTTAAGAGCACTTGA7910.17954054869952266No Hit
GTGACTGTGTGGGAGGCTGGAGAGATGGCTCAGTGGTTATGAGCGCTGACTGCTCTTCC7860.17840565269004402No Hit
TTAACTGTGTGGGAGGTGGCTTTATGAATGAATTCTACCAAACATTTAAAGAACTAACA7820.1774977358824611No Hit
GTCCCTGTGTGGGGACCTTCAAGGCTCCACCATGTTCTCGCGGGCGAGCGTTGTCGGGC7820.1774977358824611No Hit
GTAACTGTGTGGGTCTCCCAGTATTATTCTGCCAGCATACCAGAAGTCTGGTCACTCCC7770.17636283987298246No Hit
CGTACTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG7750.17590888146919098No Hit
CATCCTGTGTGGGTAACTGATGAGGGCTAAGTAAATTTGAATGAAATTTTCTTTTATTT7660.1738660686521294No Hit
CAATCTGTGTGGGGGATATAAAAGTTTGTCGCCGCTGTCTTGTTAGGGGGACATTTCGC7650.17363908945023368No Hit
ATCGCTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC7370.16728367179715323No Hit
ATATCTGTGTGGGACACCAGGTCCCACCAGCCCCTGGACTGTGGTGTCCAGTGCATATC7090.16092825414407277No Hit
GCTACTGTGTGGGGGCAAGGTTCTAAGTTAATCTTTTTTTGTGAATTGCTTTGGTACCT7030.1595663789326984No Hit
GGTACTGTGTGGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC6890.15638867010615817No Hit
TGTGCTGTGTGGGGCCATATAAAGTCTATAGCCTATTTCTGTACCTGTTGTGATCCTCT6740.1529839820777222No Hit
AATACTGTGTGGGCGCTCCCAGGGGGGCAGCAGTCTGCAGGATGGCTCGTTGGAGCTCA6660.15116814846255638No Hit
GCCACTGTGTGGGGGGAGTCTGGAAAGTTAGAAAGTCTGGGAGGGCGGCAGCGGCGGAG6660.15116814846255638No Hit
TTCACTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGC6460.14662856442464176No Hit
GATTCTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCA6450.14640158522274604No Hit
GGCCCTGTGTGGGAGAAGAGTAGAAGGAAGACTAACAGAATCAACTAACCTGGATCCTT6440.1461746060208503No Hit
TTTCCTGTGTGGGTCCCATCCTGATCACCATCATAGCCATGAGTGCCCTGGGAGTACTC6420.14572064761705886No Hit
GGGACTGTGTGGGATTCTACTGCTGAGCTGTGCCTAGCCACGGAGACTTCCTGTTCAGT6400.1452666892132674No Hit
TTAGCTGTGTGGGTTCTGCTGAGTCAAACATCTGGTATGGGCAGTGTCATCTGTATAGG6320.14345085559810156No Hit
ATTACTGTGTGGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGC6260.14208898038672715No Hit
GTTTCTGTGTGGGCTGTGTGGGTAGCTACCTAACATTTTCCCGGCAGGAAAACTAACTT6260.14208898038672715No Hit
CTTACTGTGTGGGCGGGGCTTCAGGCCTGCCTCCGAGGAGATCAAGACCCTCCAGAGGT6200.14072710517535278No Hit
GCCGCTGTGTGGGATCCAGGACACATGGTGTGCCACGCTTATCCAGCCTCCACCCACTG6080.13800335475260403No Hit
GATACTGTGTGGGACCTTGCTGCCTCTTAAAAACTGTGTGTGTGTGTGTGATTGATAGA6010.1364145003393339No Hit
CGTTCTGTGTGGGAAGCAATGGTATCAACGCAGAATCTAGCTGTGTGGGGGGCTGGCAA6010.1364145003393339No Hit
GCTTCTGTGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG5930.13459866672416806No Hit
TATGCTGTGTGGGAGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTT5890.13369074991658514No Hit
GCTACTGTGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACT5880.1334637707146894No Hit
AGGACTGTGTGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACATGCA5710.129605124282462No Hit
TGAGCTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT5670.1286972074748791No Hit
GAATCTGTGTGGGGCTTTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGGTTTTTTA5630.12778929066729616No Hit
AGACCTGTGTGGGAACCTGTTAGGCTATCTCTAATGACTATAGAGGGAGCACCTTGAAG5610.1273353322635047No Hit
GGCTCTGTGTGGGAGCTTTTGCATAGCACTCTTTTTTTTTTAGATTTATTTATTTATTA5580.1266543946578175No Hit
TGAACTGTGTGGGGAGGCCAGGCCGGGCGGCGGCTCACCAGGACGAACCATGGACAGCC5580.1266543946578175No Hit
ATTGCTGTGTGGGCAGGGTTCTCTAAGTGAACAGCATTGGTAGAATGAATATACAGGAC5560.12620043625402602No Hit
AGCACTGTGTGGGGGTCGCTAGGTTTGTTGAAGGACATCGGCCGCCGAGTTTGGGGTTC5540.12574647785023457No Hit
CGTGCTGTGTGGGCAGTGACAATGAGGAGGAGCTGGTAGAGAGACTTGAGAGTGAGGAA5470.12415762343696446No Hit
AGTTCTGTGTGGGAGACTGTCTTTGCTTCATCATCTGAAGGTAAAATTTTCCAGCCACG5360.12166085221611143No Hit
TCCGCTGTGTGGGCAGAGTTGTGCTGTGTGGGATATTGACCGATTGTCAGAGAAGCAGT5320.12075293540852851No Hit
GTAACTGTGTGGGAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATAC5320.12075293540852851No Hit
AACTCTGTGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG5190.11780220578388402No Hit
TCACCTGTGTGGGGTACCTGTGTATTTATCCTTGATCATAGTTTGCTTATTTATGTTTA5180.1175752265819883No Hit
TTAGCTGTGTGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGC5110.11598637216871817No Hit
TCACCTGTGTGGGAGAGGTGGCATGCAGATCTTTGTGAAGACCCTGACAGGCAAGACCA4920.11167376733269932No Hit
AGTCCTGTGTGGGGAGTCTGTGCAATGCTTTAATGCAGCAAGGAACAAAGAACTCACAT4910.11144678813080357No Hit
ATACCTGTGTGGGGAAGCCTCACCGCTACAGGCCAGGGACCGTGGCTCTGAGAGAGATC4820.109403975313742No Hit
TAGTCTGTGTGGGGGTAAAGGCGCTTGCCACCAAGCCTGGAGACCTGAGTTTAGTCCCA4820.109403975313742No Hit
GGCGCTGTGTGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTACATGGTAGTTCA4790.1087230377080548No Hit
TGATCTGTGTGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATT4770.10826907930426335No Hit
CAAACTGTGTGGGACGAAACCAGTTTTCTTTGCCGACAATAACTGGCACCACAAGACAA4760.10804210010236762No Hit
CGTTCTGTGTGGGGGTGCTGGCCATCCTCAGCACACTTTTAATTGGACTGCTCTTAGTG4690.10645324568909752No Hit
CCTACTGTGTGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCG4680.10622626648720176No Hit
GCTGCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT4650.10554532888151459No Hit
TGGACTGTGTGGGGAGGTCTCCAGTGCCGAGCACAGGCACCTGCCATCAGTAGTTCCTG4640.10531834967961884No Hit
AATTCTGTGTGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATC4640.10531834967961884No Hit
CTAGCTGTGTGGGGCATTCAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGGTATTA4610.10463741207393167No Hit
TGTGCTGTGTGGGCAAGCAGCTCTACCAGGTTCTCACAGTAAATATTGGGGGAAAATGT4550.10327553686255728No Hit
GGTGCTGTGTGGGAGCTTAAGGTTTTAAACTTAGAAAGACACTAGAGTGCCTGCTCATG4500.10214064085307864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGTC457.6397555E-1153.00000450
AGGTATG457.6397555E-1153.00000436
CGGGTAG457.6397555E-1153.00000415
TAGAGGC1950.053.00000426
CTGTCGA1500.053.00000439
CTCACGT1800.053.00000433
GATACAT457.6397555E-1153.00000447
CTCACCG457.6397555E-1153.00000419
CTCACAT1500.053.00000453
CGAACTC457.6397555E-1153.00000445
AATTTTC1500.053.00000445
CTGTATA900.053.00000451
ATGATCT457.6397555E-1153.00000426
CATATAA900.053.00000416
TCCGCAC457.6397555E-1153.00000449
CTACTAA2700.053.00000441
TTGTGAT900.053.00000448
CTCCGCA457.6397555E-1153.00000448
ATGGCTA457.6397555E-1153.00000426
ACGTTCC1800.053.00000436
Produced by FastQC (version 0.11.5)