SRR1528116_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1442561
Sequences flagged as poor quality	0
Sequence length	52
%GC	36

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTTAACTTATGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAA	3904	0.2706298035230399	No Hit
CTTTACTTATGGGGGGTGGAGTGCAGTGGCTCTATCTCAGCTCACTGCAACC	2760	0.191326397982477	No Hit
CTTCACTTATGGAGACTTGAGCCACCAGTGCTGAACACCCAGGAGGTTGCTG	2321	0.16089440931787286	No Hit
ACTGACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2245	0.15562600125748582	No Hit
TAGCACTTATGGGGTTGCTTTTCTTTTGGAATGGCCCTGTGGTCACAGAGCT	2215	0.15354636649680672	No Hit
TTTCACTTATGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC	2185	0.15146673173612762	No Hit
TGCTACTTATGGGGCTGAGGCGGGCAGATCACTTGAGGTCACGAGTTTGAGA	1960	0.1358694710310344	No Hit
GGCCACTTATGGAGAGTATAAACTTATGGGGGCTGGTGAGATGGCTCAGTGG	1949	0.13510693828545206	No Hit
GACGACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGC	1897	0.13150223803360828	No Hit
AGCGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT	1865	0.1292839609555506	No Hit
CAATACTTATGGGCCTTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCC	1847	0.1280361800991431	No Hit
AGTTACTTATGGGAAGGAGGCTGCTGTTTGCAACCATTGCACACCAAAGTCC	1807	0.125263333751571	No Hit
TAGTACTTATGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT	1802	0.12491672795812447	No Hit
TAAAACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA	1743	0.12082677959545558	No Hit
GGGCACTTATGGGAAAGTGTTACGACCACTTCATCAGATGAAGTGTTACCAT	1714	0.11881646599346579	No Hit
TTGCACTTATGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT	1670	0.11576633501113644	No Hit
TTGGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1647	0.11417194836128247	No Hit
AATTACTTATGGGAGTATCCTTCAGGATGCCCTCTGAGGGCTGATAGAGATC	1591	0.11028996347468148	No Hit
ATTGACTTATGGGCAATCTTACCTCAAGAAACAAGAAAAATTTCAGATAAAC	1524	0.10564544584249816	No Hit
AGGCACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC	1511	0.10474427077953723	No Hit
AGTGACTTATGGGCAGCACTCAGCAGGCTGCCATGACAGCAGGCTTTCCTCC	1503	0.10418970151002281	No Hit
GCTAACTTATGGGGAGACCACCCTGGCCAACATGGTGAAACCCCGTCTCTAC	1497	0.10377377455788699	No Hit
CAAAACTTATGGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGC	1487	0.10308056297099394	No Hit
GGCCACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT	1470	0.10190210327327578	No Hit
CTTTACTTATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1468	0.10176346095589718	No Hit
CAGGACTTATGGGGACGACTGGCAGGAGGCGGAGATGACAGACCGCTCCAGG	1457	0.10100092821031484	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTATC	20	6.3119916E-4	46.000004	41
ATCCTAG	80	0.0	46.000004	40
CTAGACC	155	0.0	46.000004	46
TCGTTAA	20	6.3119916E-4	46.000004	25
CTCACCG	40	5.6134013E-9	46.000004	35
ATCTCAA	20	6.3119916E-4	46.000004	30
GCCCAAT	80	0.0	46.000004	14
TCACCGT	40	5.6134013E-9	46.000004	36
GATCGTT	20	6.3119916E-4	46.000004	23
GATGTAC	40	5.6134013E-9	46.000004	13
ATTAGCC	40	5.6134013E-9	46.000004	16
TTGTGCC	40	5.6134013E-9	46.000004	31
CTCCGAA	40	5.6134013E-9	46.000004	23
CGTGCTA	40	5.6134013E-9	46.000004	40
TGAAGGT	40	5.6134013E-9	46.000004	15
ACGTTCT	40	5.6134013E-9	46.000004	17
CCGATCC	160	0.0	46.000004	42
TTAGCGA	20	6.3119916E-4	46.000004	38
TCATTCA	40	5.6134013E-9	46.000004	22
CGTGCAC	80	0.0	46.000004	40

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content