##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1442561
Sequences flagged as poor quality	0
Sequence length	52
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.723101484096688	31.0	31.0	34.0	27.0	34.0
2	31.580118275760956	31.0	31.0	34.0	28.0	34.0
3	31.861292520732224	31.0	31.0	34.0	30.0	34.0
4	35.50743157481729	37.0	35.0	37.0	33.0	37.0
5	35.23682326085344	37.0	35.0	37.0	33.0	37.0
6	35.56513520052185	37.0	35.0	37.0	33.0	37.0
7	35.719588287774314	37.0	35.0	37.0	33.0	37.0
8	35.73258461860539	37.0	35.0	37.0	33.0	37.0
9	37.19274540210085	39.0	37.0	39.0	33.0	39.0
10	37.623260992082834	39.0	37.0	39.0	35.0	39.0
11	37.79751428189172	39.0	37.0	39.0	35.0	39.0
12	37.64975761856864	39.0	37.0	39.0	35.0	39.0
13	37.53538325242399	39.0	37.0	39.0	35.0	39.0
14	37.784373069838985	40.0	38.0	40.0	33.0	40.0
15	36.99578943282121	39.0	36.0	40.0	31.0	40.0
16	36.72398463565839	38.0	35.0	40.0	31.0	40.0
17	36.47376506088824	39.0	35.0	40.0	31.0	40.0
18	36.666954811616286	39.0	35.0	40.0	31.0	40.0
19	36.85557491156353	39.0	35.0	40.0	31.0	40.0
20	36.954051163174384	39.0	35.0	40.0	32.0	40.0
21	36.896906266008855	39.0	35.0	40.0	31.0	40.0
22	36.98068019307329	39.0	35.0	40.0	31.0	40.0
23	36.948010517406196	39.0	35.0	40.0	31.0	40.0
24	36.95067452953462	39.0	35.0	40.0	31.0	40.0
25	37.04155387536471	39.0	36.0	40.0	31.0	40.0
26	36.79135579015376	39.0	35.0	40.0	31.0	40.0
27	36.57708963433782	39.0	35.0	40.0	31.0	40.0
28	36.08649339611982	38.0	35.0	40.0	30.0	40.0
29	35.72280340311432	37.0	35.0	40.0	30.0	40.0
30	35.37579346731265	37.0	34.0	40.0	29.0	40.0
31	35.239647404858445	36.0	34.0	40.0	29.0	40.0
32	35.16844764276866	36.0	34.0	40.0	29.0	40.0
33	34.9240621367138	36.0	34.0	40.0	27.0	40.0
34	34.62366166838005	36.0	34.0	40.0	25.0	40.0
35	34.4033985391259	35.0	33.0	40.0	24.0	40.0
36	34.11132492837391	35.0	33.0	40.0	23.0	40.0
37	33.81865723529196	35.0	33.0	40.0	21.0	40.0
38	33.328699444945485	35.0	33.0	40.0	18.0	40.0
39	32.78312320934782	35.0	33.0	40.0	15.0	40.0
40	32.13409900863811	35.0	31.0	40.0	12.0	40.0
41	31.41147098805527	35.0	30.0	40.0	10.0	40.0
42	30.65051945810264	35.0	26.0	39.0	9.0	40.0
43	29.9430228600385	35.0	23.0	39.0	8.0	40.0
44	29.296831121872835	35.0	21.0	39.0	8.0	40.0
45	28.866960911878248	35.0	20.0	39.0	8.0	40.0
46	28.449944231127834	35.0	17.0	38.0	8.0	40.0
47	27.928592967645734	35.0	15.0	38.0	8.0	40.0
48	27.529166530912732	34.0	13.0	38.0	8.0	40.0
49	27.251846542364586	34.0	11.0	37.0	8.0	40.0
50	26.889559609610963	33.0	10.0	37.0	8.0	40.0
51	26.51455917635372	33.0	10.0	36.0	8.0	40.0
52	25.694909262069334	33.0	10.0	35.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	8.0
15	30.0
16	91.0
17	320.0
18	807.0
19	1912.0
20	3823.0
21	6261.0
22	9819.0
23	13863.0
24	19505.0
25	26617.0
26	35457.0
27	46401.0
28	59258.0
29	74646.0
30	85909.0
31	84365.0
32	71822.0
33	68021.0
34	83901.0
35	121053.0
36	143339.0
37	182500.0
38	249830.0
39	52999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.17264295929254	23.71227282589783	37.70585784587272	6.4092263689369116
2	19.75458923400813	22.09050431836158	36.99268176527717	21.16222468235312
3	20.288639440550522	21.81904266093427	39.41004921109055	18.482268687424657
4	22.125858109293127	22.794391363692764	32.54046102729798	22.53928949971613
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.59717474685645	0.24782314231425917	0.08665144836162908	0.06835066246765302
12	98.8593896549262	0.6530746360119261	0.16269675944379475	0.3248389496180751
13	95.58971856302783	2.920015167469521	0.7001437027619629	0.7901225667406786
14	47.73323277143913	33.47088961922581	10.947613307166906	7.8482643021681575
15	29.02317475656142	43.87752060398139	18.100309103046598	8.998995536410593
16	32.36230564946647	40.93248049822503	15.645300268064922	11.059913584243578
17	18.139475557706053	42.62876925135228	18.90069120127329	20.331063989668372
18	16.00937499350114	50.100342377202765	20.317754327200028	13.572528302096066
19	21.586331531214277	48.214876182012404	19.765957904033176	10.43283438274014
20	17.101737812127183	48.30991549057544	21.79110623398248	12.797240463314896
21	20.301047927955906	48.05204078025123	14.809425736589303	16.837485555203557
22	22.315867405260505	51.25356917315802	15.14597996202587	11.28458345955561
23	13.684689936855357	50.840692352004524	18.669574458203154	16.80504325293696
24	15.959047832292708	55.632378804085235	14.649640465810457	13.7589328978116
25	18.133028689947945	49.637069073682156	18.325117620676007	13.904784615693893
26	16.018248101813374	51.329822447716246	16.205761836067936	16.44616761440244
27	18.117223465766784	53.451049903608926	16.136717962013392	12.295008668610894
28	19.886230114359115	51.702978244940766	14.077810227782395	14.33298141291772
29	19.489227838545474	51.208649062327346	13.2754871371124	16.02663596201478
30	20.99717100351389	51.405451831846285	15.422086137085364	12.175291027554467
31	16.980287142103524	54.120137727278085	13.45939617111512	15.440178959503273
32	16.39078000860969	59.41967098791663	14.091050569092053	10.09849843438163
33	19.461568696228444	57.22447785570246	12.696170213945893	10.617783234123202
34	16.098314040099517	56.170103032038156	14.323068487225148	13.408514440637173
35	20.22514125919112	53.60632929907296	15.986914938085809	10.181614503650106
36	17.414237595498562	54.20096619830981	17.41707976300482	10.967716443186806
37	17.59267025796483	55.49616272726075	15.340980381418879	11.57018663335554
38	14.84533409679036	53.83335609378044	20.47837145188314	10.842938357546059
39	17.67606361186806	55.527842496781766	16.30891172019762	10.487182171152554
40	19.59743816725948	55.64970909375756	13.625697630810759	11.127155108172202
41	22.345606182338216	52.03717555098189	13.465427111921091	12.151791154758794
42	20.913500364975903	49.71560994647714	14.304629059013795	15.06626062953317
43	23.767591110531892	47.26122500192366	16.283817460752093	12.687366426792352
44	21.960873751612585	47.604226095118335	16.05311664463409	14.38178350863499
45	21.478259844817654	49.67325471851797	14.521327001076559	14.327158435587819
46	24.394531669717953	45.36549927524728	15.880922886449863	14.3590461685849
47	25.89859284979977	45.80215325383121	13.742642425519612	14.556611470849411
48	26.65717428933681	45.135283707240106	13.439085071619155	14.768456931803925
49	25.866774437961375	47.22372225507275	12.701022695054142	14.20848061191173
50	22.8207334039947	49.69668527015496	12.975049235352959	14.507532090497385
51	21.356670532476617	48.42582046790396	14.048764662291576	16.16874433732785
52	23.790397771740675	45.62476040874528	15.000266886460953	15.58457493305309
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	4.0
2	8.0
3	17.0
4	26.0
5	66.0
6	106.0
7	2100.0
8	4094.0
9	9800.0
10	15506.0
11	19249.0
12	22992.0
13	22620.0
14	20520.5
15	18793.0
16	19911.0
17	21029.0
18	25434.5
19	29840.0
20	38904.0
21	47968.0
22	59036.0
23	70104.0
24	79646.5
25	89189.0
26	90821.5
27	92454.0
28	86777.5
29	81101.0
30	75951.5
31	70802.0
32	66333.0
33	61864.0
34	58625.5
35	55387.0
36	55469.0
37	55551.0
38	59622.5
39	67066.5
40	70439.0
41	69536.5
42	68634.0
43	68889.5
44	69145.0
45	73923.0
46	78701.0
47	71877.0
48	65053.0
49	57484.5
50	49916.0
51	47144.5
52	44373.0
53	44320.0
54	44267.0
55	42421.5
56	40576.0
57	34721.0
58	28866.0
59	24279.5
60	19693.0
61	18410.0
62	17127.0
63	12943.0
64	7511.0
65	6263.0
66	5425.5
67	4588.0
68	3400.0
69	2212.0
70	1634.5
71	1057.0
72	575.5
73	94.0
74	60.5
75	27.0
76	18.0
77	9.0
78	7.0
79	5.0
80	2.5
81	0.0
82	0.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1442561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.812517174179106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.58058397194831	38.809383565698
2	5.059136890965775	4.433070438439222
3	1.6202835563405917	2.1296610341763653
4	0.7954692354556095	1.3940603815972006
5	0.5130417654653271	1.1238825580260405
6	0.36266652862492565	0.9533600108327686
7	0.29452360972992475	0.9032674496645486
8	0.20596140757662978	0.7218950165335356
9	0.19676816179534962	0.7758817621191367
>10	2.0853536055607607	19.38882555866485
>50	0.1525854101687255	4.459525107970781
>100	0.09597794544489816	9.65126361375807
>500	0.02569106367723604	7.8198784145946565
>1k	0.011956847246009228	7.436045087924802
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTAACTTATGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAA	3904	0.2706298035230399	No Hit
CTTTACTTATGGGGGGTGGAGTGCAGTGGCTCTATCTCAGCTCACTGCAACC	2760	0.191326397982477	No Hit
CTTCACTTATGGAGACTTGAGCCACCAGTGCTGAACACCCAGGAGGTTGCTG	2321	0.16089440931787286	No Hit
ACTGACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2245	0.15562600125748582	No Hit
TAGCACTTATGGGGTTGCTTTTCTTTTGGAATGGCCCTGTGGTCACAGAGCT	2215	0.15354636649680672	No Hit
TTTCACTTATGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC	2185	0.15146673173612762	No Hit
TGCTACTTATGGGGCTGAGGCGGGCAGATCACTTGAGGTCACGAGTTTGAGA	1960	0.1358694710310344	No Hit
GGCCACTTATGGAGAGTATAAACTTATGGGGGCTGGTGAGATGGCTCAGTGG	1949	0.13510693828545206	No Hit
GACGACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGC	1897	0.13150223803360828	No Hit
AGCGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT	1865	0.1292839609555506	No Hit
CAATACTTATGGGCCTTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCC	1847	0.1280361800991431	No Hit
AGTTACTTATGGGAAGGAGGCTGCTGTTTGCAACCATTGCACACCAAAGTCC	1807	0.125263333751571	No Hit
TAGTACTTATGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT	1802	0.12491672795812447	No Hit
TAAAACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA	1743	0.12082677959545558	No Hit
GGGCACTTATGGGAAAGTGTTACGACCACTTCATCAGATGAAGTGTTACCAT	1714	0.11881646599346579	No Hit
TTGCACTTATGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT	1670	0.11576633501113644	No Hit
TTGGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1647	0.11417194836128247	No Hit
AATTACTTATGGGAGTATCCTTCAGGATGCCCTCTGAGGGCTGATAGAGATC	1591	0.11028996347468148	No Hit
ATTGACTTATGGGCAATCTTACCTCAAGAAACAAGAAAAATTTCAGATAAAC	1524	0.10564544584249816	No Hit
AGGCACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC	1511	0.10474427077953723	No Hit
AGTGACTTATGGGCAGCACTCAGCAGGCTGCCATGACAGCAGGCTTTCCTCC	1503	0.10418970151002281	No Hit
GCTAACTTATGGGGAGACCACCCTGGCCAACATGGTGAAACCCCGTCTCTAC	1497	0.10377377455788699	No Hit
CAAAACTTATGGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGC	1487	0.10308056297099394	No Hit
GGCCACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT	1470	0.10190210327327578	No Hit
CTTTACTTATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1468	0.10176346095589718	No Hit
CAGGACTTATGGGGACGACTGGCAGGAGGCGGAGATGACAGACCGCTCCAGG	1457	0.10100092821031484	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.025648828715042205	0.0	0.0	0.0	0.0
12	0.07729309193857313	0.0	0.0	0.0	0.0
13	0.20581452014854137	0.0	0.0	0.0	0.0
14	0.6762279030141533	0.0	0.0	0.0	0.0
15	1.05562260452071	0.0	0.0	0.0	0.0
16	1.355991185121461	0.0	0.0	0.0	0.0
17	1.5688764634563115	0.0	0.0	0.0	0.0
18	1.7998545642090698	0.0	0.0	0.0	0.0
19	1.9987369684886809	0.0	0.0	0.0	0.0
20	2.2007388249093105	0.0	0.0	0.0	0.0
21	2.394214178811156	0.0	0.0	0.0	0.0
22	2.5878974961890693	0.0	0.0	0.0	0.0
23	2.7760351208718386	0.0	0.0	0.0	0.0
24	3.000843638501249	0.0	0.0	0.0	0.0
25	3.238615212805559	0.0	0.0	0.0	0.0
26	3.5122951473109283	0.0	0.0	0.0	0.0
27	3.767119726652807	0.0	0.0	0.0	0.0
28	4.0335902606544884	0.0	0.0	0.0	0.0
29	4.334582731683444	0.0	0.0	0.0	0.0
30	4.681327167447338	0.0	0.0	0.0	0.0
31	4.975248880289985	0.0	0.0	0.0	0.0
32	5.199294865173813	0.0	0.0	0.0	0.0
33	5.39152243821925	0.0	0.0	0.0	0.0
34	5.5721733777635745	0.0	0.0	0.0	0.0
35	5.7326518601293115	0.0	0.0	0.0	0.0
36	5.888208540228108	0.0	0.0	0.0	0.0
37	6.041408300931469	0.0	0.0	0.0	0.0
38	6.190934040224295	0.0	0.0	0.0	0.0
39	6.343925837451588	0.0	0.0	0.0	0.0
40	6.50183943694582	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTATC	20	6.3119916E-4	46.000004	41
ATCCTAG	80	0.0	46.000004	40
CTAGACC	155	0.0	46.000004	46
TCGTTAA	20	6.3119916E-4	46.000004	25
CTCACCG	40	5.6134013E-9	46.000004	35
ATCTCAA	20	6.3119916E-4	46.000004	30
GCCCAAT	80	0.0	46.000004	14
TCACCGT	40	5.6134013E-9	46.000004	36
GATCGTT	20	6.3119916E-4	46.000004	23
GATGTAC	40	5.6134013E-9	46.000004	13
ATTAGCC	40	5.6134013E-9	46.000004	16
TTGTGCC	40	5.6134013E-9	46.000004	31
CTCCGAA	40	5.6134013E-9	46.000004	23
CGTGCTA	40	5.6134013E-9	46.000004	40
TGAAGGT	40	5.6134013E-9	46.000004	15
ACGTTCT	40	5.6134013E-9	46.000004	17
CCGATCC	160	0.0	46.000004	42
TTAGCGA	20	6.3119916E-4	46.000004	38
TCATTCA	40	5.6134013E-9	46.000004	22
CGTGCAC	80	0.0	46.000004	40
>>END_MODULE