SRR1528115_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528115_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1436862
Sequences flagged as poor quality	0
Sequence length	52
%GC	36

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTTAACTTATGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAA	3743	0.260498224603337	No Hit
CTTTACTTATGGGGGGTGGAGTGCAGTGGCTCTATCTCAGCTCACTGCAACC	2883	0.2006455734788727	No Hit
CTTCACTTATGGAGACTTGAGCCACCAGTGCTGAACACCCAGGAGGTTGCTG	2493	0.17350309215498774	No Hit
ACTGACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2286	0.1590966982215411	No Hit
TTTCACTTATGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC	2234	0.1554777007116898	No Hit
TAGCACTTATGGGGTTGCTTTTCTTTTGGAATGGCCCTGTGGTCACAGAGCT	2187	0.15220668373163185	No Hit
TGCTACTTATGGGGCTGAGGCGGGCAGATCACTTGAGGTCACGAGTTTGAGA	1949	0.13564281051346613	No Hit
GGCCACTTATGGAGAGTATAAACTTATGGGGGCTGGTGAGATGGCTCAGTGG	1934	0.13459886892408596	No Hit
AGCGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT	1897	0.13202381300361482	No Hit
GACGACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGC	1892	0.13167583247382142	No Hit
AGTTACTTATGGGAAGGAGGCTGCTGTTTGCAACCATTGCACACCAAAGTCC	1794	0.12485541408987086	No Hit
CAATACTTATGGGCCTTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCC	1775	0.12353308807665593	No Hit
TAGTACTTATGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT	1750	0.12179318542768895	No Hit
TAAAACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA	1724	0.1199836866727633	No Hit
TTGCACTTATGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT	1682	0.11706065022249876	No Hit
TTGGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1653	0.11504236314969704	No Hit
GGGCACTTATGGGAAAGTGTTACGACCACTTCATCAGATGAAGTGTTACCAT	1634	0.11372003713648214	No Hit
AGGCACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC	1548	0.10773477202403571	No Hit
AATTACTTATGGGAGTATCCTTCAGGATGCCCTCTGAGGGCTGATAGAGATC	1541	0.10724759928232497	No Hit
GGCCACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT	1517	0.10557729273931665	No Hit
ATTGACTTATGGGCAATCTTACCTCAAGAAACAAGAAAAATTTCAGATAAAC	1504	0.1046725433618538	No Hit
GCTAACTTATGGGGAGACCACCCTGGCCAACATGGTGAAACCCCGTCTCTAC	1462	0.1017495069115893	No Hit
CAAAACTTATGGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGC	1461	0.1016799108056306	No Hit
CTTTACTTATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1445	0.10056637311029173	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTGT	55	1.8189894E-12	46.000004	46
CTTAACG	35	1.0198164E-7	46.000004	31
CTGCTCA	55	1.8189894E-12	46.000004	39
ACAACAC	55	1.8189894E-12	46.000004	45
TTCTAAG	55	1.8189894E-12	46.000004	18
CTAGACC	140	0.0	46.000004	46
AGCGTCA	55	1.8189894E-12	46.000004	39
TAATAGG	70	0.0	46.000004	41
CTGTCTC	140	0.0	46.000004	25
GACAGAC	155	0.0	46.000004	37
CCTTAGG	55	1.8189894E-12	46.000004	27
ACAGTGT	35	1.0198164E-7	46.000004	29
GCCTTCG	145	0.0	46.000004	26
ACCGGTT	55	1.8189894E-12	46.000004	43
GCCTTAG	55	1.8189894E-12	46.000004	26
ACTGCAT	55	1.8189894E-12	46.000004	27
TCACCGA	35	1.0198164E-7	46.000004	36
CTCAATT	35	1.0198164E-7	46.000004	19
TAAAGTA	35	1.0198164E-7	46.000004	42
TCCTGTG	70	0.0	46.000004	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content