##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528115_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1436862
Sequences flagged as poor quality	0
Sequence length	52
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.75447537759367	31.0	31.0	34.0	27.0	34.0
2	31.604535439033114	31.0	31.0	34.0	28.0	34.0
3	31.880552899304178	31.0	31.0	34.0	30.0	34.0
4	35.546346134841066	37.0	35.0	37.0	33.0	37.0
5	35.37382573970221	37.0	35.0	37.0	33.0	37.0
6	35.61356483782019	37.0	35.0	37.0	33.0	37.0
7	35.70889410395709	37.0	35.0	37.0	33.0	37.0
8	35.744185593327686	37.0	35.0	37.0	33.0	37.0
9	37.18028871248596	39.0	37.0	39.0	33.0	39.0
10	37.61110252759138	39.0	37.0	39.0	35.0	39.0
11	37.78603025203534	39.0	37.0	39.0	35.0	39.0
12	37.658906004891215	39.0	37.0	39.0	35.0	39.0
13	37.524878519997046	39.0	37.0	39.0	35.0	39.0
14	37.73966045451825	40.0	38.0	40.0	33.0	40.0
15	36.9913756505496	39.0	36.0	40.0	31.0	40.0
16	36.69552886776879	38.0	35.0	40.0	31.0	40.0
17	36.48307631491403	39.0	35.0	40.0	31.0	40.0
18	36.584170922468545	39.0	35.0	40.0	31.0	40.0
19	36.85532848666051	39.0	35.0	40.0	31.0	40.0
20	36.958625115007564	39.0	35.0	40.0	32.0	40.0
21	36.95206916182626	39.0	35.0	40.0	31.0	40.0
22	36.909154810970016	39.0	35.0	40.0	31.0	40.0
23	36.96253641616244	39.0	35.0	40.0	31.0	40.0
24	37.0648858415074	39.0	36.0	40.0	32.0	40.0
25	37.07894912663846	40.0	36.0	40.0	32.0	40.0
26	36.716327664034544	39.0	35.0	40.0	31.0	40.0
27	36.46061138787162	39.0	35.0	40.0	30.0	40.0
28	36.028439752738954	38.0	35.0	40.0	30.0	40.0
29	35.65402453401927	37.0	35.0	40.0	30.0	40.0
30	35.195354877503895	36.0	34.0	40.0	29.0	40.0
31	35.11651153694648	36.0	34.0	40.0	29.0	40.0
32	35.13013219084366	36.0	34.0	40.0	28.0	40.0
33	34.98216669380915	36.0	34.0	40.0	27.0	40.0
34	34.72364082284868	36.0	34.0	40.0	25.0	40.0
35	34.325749445667014	35.0	33.0	40.0	24.0	40.0
36	34.07959358657964	35.0	33.0	40.0	23.0	40.0
37	33.78981488827737	35.0	33.0	40.0	21.0	40.0
38	33.34144823928811	35.0	33.0	40.0	19.0	40.0
39	32.79912336744935	35.0	33.0	40.0	15.0	40.0
40	32.09101361160641	35.0	31.0	40.0	12.0	40.0
41	31.33818835768501	35.0	30.0	39.0	10.0	40.0
42	30.568732418283734	35.0	26.0	39.0	9.0	40.0
43	29.8991461949721	35.0	23.0	39.0	8.0	40.0
44	29.193739551884594	35.0	20.0	39.0	8.0	40.0
45	28.76621415278572	35.0	20.0	38.0	8.0	40.0
46	28.3505360988042	35.0	17.0	38.0	8.0	40.0
47	27.935853965098946	35.0	15.0	38.0	8.0	40.0
48	27.53279298916667	34.0	13.0	38.0	8.0	40.0
49	27.09285930033643	33.0	10.0	37.0	8.0	40.0
50	26.74171562752721	33.0	10.0	37.0	8.0	40.0
51	26.36968755524191	33.0	10.0	36.0	8.0	40.0
52	25.388744360975515	33.0	10.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	7.0
15	24.0
16	110.0
17	306.0
18	806.0
19	1868.0
20	3642.0
21	6314.0
22	9702.0
23	13968.0
24	19933.0
25	26719.0
26	36120.0
27	46882.0
28	59524.0
29	74658.0
30	86433.0
31	83627.0
32	71268.0
33	68128.0
34	84629.0
35	119821.0
36	142906.0
37	183086.0
38	249083.0
39	47295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.08136898324265	23.75301177148536	37.6807236881482	6.484895557123788
2	19.765015707841115	22.079503807602958	37.087277692638544	21.068202791917386
3	20.27258010859776	21.791793505569775	39.457999446014995	18.477626939817462
4	22.09739000683434	22.718256868091714	32.56812414831765	22.616228976756293
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.59648177765158	0.24880607880227884	0.0851160375874649	0.0695961059586794
12	98.90622759875339	0.626712934157908	0.1526242603673839	0.31443520672131353
13	95.5926873979547	2.935772537654973	0.6909501399577691	0.7805899244325482
14	47.77222864826267	33.446496601622144	10.964588109366105	7.816686640749077
15	29.06256829117897	43.893498470973555	18.013142528649237	9.03079070919824
16	32.36740897873282	40.90921744746538	15.720785990582256	11.002587583219544
17	18.106540502845785	42.718785798497	18.840501036286017	20.334172662371195
18	16.014272769410006	50.13786988590414	20.31545130986831	13.53240603481754
19	21.630052155321806	48.26121088872836	19.718177528530923	10.390559427418918
20	17.1223819684841	48.35864543707051	21.74314582750466	12.775826766940737
21	20.316495251457688	48.03857294576654	14.744839796723694	16.900092006052077
22	22.287317779995576	51.265535590752634	15.155178437456069	11.291968191795734
23	13.666030558258205	50.83800671184846	18.67061694164088	16.825345788252456
24	15.883571282419606	55.70054744296947	14.612537599296244	13.803343675314677
25	18.10828040549475	49.69663057412612	18.280530767742484	13.914558252636649
26	15.954698502709377	51.37180884455152	16.189446168108002	16.484046484631094
27	18.10048564162738	53.34458006405626	16.20900267388239	12.345931620433975
28	19.90852287832791	51.762034210661845	14.042197510964865	14.287245400045375
29	19.46693558602009	51.22878884680644	13.285827031405939	16.018448535767526
30	20.965130958992585	51.42832088258997	15.458756651647827	12.147791506769613
31	16.8946635097873	54.14959822168029	13.502549305361267	15.453188963171133
32	16.38856062725578	59.47676255618146	14.029183039150595	10.105493777412168
33	19.471876909543155	57.265903058192094	12.617704414202615	10.644515618062139
34	16.026521684058732	56.30700791029341	14.262399590218127	13.404070815429737
35	19.985704959836088	53.673630453028885	16.21491834288888	10.125746244246143
36	17.33959141518114	54.28454507113418	17.4078651951266	10.96799831855808
37	17.56306451141446	55.495308526497325	15.367655348947915	11.573971613140301
38	14.836358676059358	53.84817748677327	20.432859940620602	10.88260389654678
39	17.706989258537007	55.53379517309247	16.278181203205317	10.4810343651652
40	19.590607866308666	55.65287411038777	13.652946490337973	11.103571532965589
41	22.352529331278856	52.00109683462991	13.493014638844928	12.153359195246308
42	20.91418660943083	49.62056203031328	14.317171725607608	15.148079634648282
43	23.879259107694406	47.175929212408704	16.242826381378308	12.701985298518577
44	21.923121357513807	47.55724627695631	16.211577729802862	14.308054635727022
45	21.394678124969552	49.668165766789016	14.58581269460811	14.35134341363332
46	24.30066352927421	45.47089421252702	15.890043720273765	14.338398537925007
47	26.024141497234947	45.84754833797539	13.610562461809137	14.517747702980524
48	26.652037565194153	45.13968634426966	13.371778222264908	14.836497868271275
49	25.64094533782646	47.23223246212928	12.930747698804756	14.196074501239506
50	22.867401323161165	49.66252848220636	12.998186325478716	14.471883869153753
51	21.37797505953947	48.37687961683168	14.056255924368518	16.188889399260333
52	23.938415797759284	45.460176412209385	15.052454585061056	15.548953204970276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	16.0
4	31.0
5	51.0
6	71.0
7	2093.0
8	4115.0
9	9779.0
10	15443.0
11	19067.5
12	22692.0
13	22204.5
14	20137.5
15	18558.0
16	19888.5
17	21219.0
18	25516.0
19	29813.0
20	38584.0
21	47355.0
22	58520.0
23	69685.0
24	79631.0
25	89577.0
26	91022.0
27	92467.0
28	86670.0
29	80873.0
30	76027.0
31	71181.0
32	66393.0
33	61605.0
34	58418.0
35	55231.0
36	55280.0
37	55329.0
38	59554.0
39	67098.0
40	70417.0
41	69220.0
42	68023.0
43	68586.5
44	69150.0
45	73970.0
46	78790.0
47	71536.5
48	64283.0
49	56874.0
50	49465.0
51	46558.0
52	43651.0
53	43893.5
54	44136.0
55	41941.0
56	39746.0
57	34341.5
58	28937.0
59	24210.5
60	19484.0
61	18373.0
62	17262.0
63	12835.5
64	7364.0
65	6319.0
66	5464.0
67	4609.0
68	3416.5
69	2224.0
70	1642.5
71	1061.0
72	582.0
73	103.0
74	64.0
75	25.0
76	20.0
77	15.0
78	11.5
79	8.0
80	5.0
81	2.0
82	1.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1436862.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.37127140880453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.82448392835812	39.41255284132175
2	4.93460219694269	4.379091467500544
3	1.548974312888467	2.06189878927322
4	0.7912191089659949	1.404295913110506
5	0.4916108774284358	1.090669983494883
6	0.35621235398362805	0.948335702266603
7	0.29820779814843135	0.9262301403506231
8	0.22899538867487002	0.8128653233805871
9	0.20011025017277534	0.799123159988999
>10	2.044577573313612	19.163350692496277
>50	0.15024854622209594	4.394391950335731
>100	0.09333007837355203	9.455374325681627
>500	0.02580114475506215	7.866816709371024
>1k	0.01162644177234282	7.285003001427694
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTAACTTATGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAA	3743	0.260498224603337	No Hit
CTTTACTTATGGGGGGTGGAGTGCAGTGGCTCTATCTCAGCTCACTGCAACC	2883	0.2006455734788727	No Hit
CTTCACTTATGGAGACTTGAGCCACCAGTGCTGAACACCCAGGAGGTTGCTG	2493	0.17350309215498774	No Hit
ACTGACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2286	0.1590966982215411	No Hit
TTTCACTTATGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC	2234	0.1554777007116898	No Hit
TAGCACTTATGGGGTTGCTTTTCTTTTGGAATGGCCCTGTGGTCACAGAGCT	2187	0.15220668373163185	No Hit
TGCTACTTATGGGGCTGAGGCGGGCAGATCACTTGAGGTCACGAGTTTGAGA	1949	0.13564281051346613	No Hit
GGCCACTTATGGAGAGTATAAACTTATGGGGGCTGGTGAGATGGCTCAGTGG	1934	0.13459886892408596	No Hit
AGCGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT	1897	0.13202381300361482	No Hit
GACGACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGC	1892	0.13167583247382142	No Hit
AGTTACTTATGGGAAGGAGGCTGCTGTTTGCAACCATTGCACACCAAAGTCC	1794	0.12485541408987086	No Hit
CAATACTTATGGGCCTTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCC	1775	0.12353308807665593	No Hit
TAGTACTTATGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT	1750	0.12179318542768895	No Hit
TAAAACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA	1724	0.1199836866727633	No Hit
TTGCACTTATGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT	1682	0.11706065022249876	No Hit
TTGGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1653	0.11504236314969704	No Hit
GGGCACTTATGGGAAAGTGTTACGACCACTTCATCAGATGAAGTGTTACCAT	1634	0.11372003713648214	No Hit
AGGCACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC	1548	0.10773477202403571	No Hit
AATTACTTATGGGAGTATCCTTCAGGATGCCCTCTGAGGGCTGATAGAGATC	1541	0.10724759928232497	No Hit
GGCCACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT	1517	0.10557729273931665	No Hit
ATTGACTTATGGGCAATCTTACCTCAAGAAACAAGAAAAATTTCAGATAAAC	1504	0.1046725433618538	No Hit
GCTAACTTATGGGGAGACCACCCTGGCCAACATGGTGAAACCCCGTCTCTAC	1462	0.1017495069115893	No Hit
CAAAACTTATGGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGC	1461	0.1016799108056306	No Hit
CTTTACTTATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1445	0.10056637311029173	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.02644652026429817	0.0	0.0	0.0	0.0
12	0.07551177496516714	0.0	0.0	0.0	0.0
13	0.2006455734788727	0.0	0.0	0.0	0.0
14	0.6660347340245618	0.0	0.0	0.0	0.0
15	1.0435936088503976	0.0	0.0	0.0	0.0
16	1.3252490496651732	0.0	0.0	0.0	0.0
17	1.5430848613158397	0.0	0.0	0.0	0.0
18	1.779850813787267	0.0	0.0	0.0	0.0
19	1.972840815610685	0.0	0.0	0.0	0.0
20	2.1731384085597645	0.0	0.0	0.0	0.0
21	2.366963563654686	0.0	0.0	0.0	0.0
22	2.5625286213985756	0.0	0.0	0.0	0.0
23	2.753430740043233	0.0	0.0	0.0	0.0
24	2.963193403402693	0.0	0.0	0.0	0.0
25	3.1941828790795497	0.0	0.0	0.0	0.0
26	3.468948305404416	0.0	0.0	0.0	0.0
27	3.724505206484687	0.0	0.0	0.0	0.0
28	3.9964171924652474	0.0	0.0	0.0	0.0
29	4.29721156241866	0.0	0.0	0.0	0.0
30	4.642547440185627	0.0	0.0	0.0	0.0
31	4.926708340814915	0.0	0.0	0.0	0.0
32	5.158532969763276	0.0	0.0	0.0	0.0
33	5.349017511772181	0.0	0.0	0.0	0.0
34	5.523077372774838	0.0	0.0	0.0	0.0
35	5.680503764453372	0.0	0.0	0.0	0.0
36	5.830483372794325	0.0	0.0	0.0	0.0
37	5.979210251228023	0.0	0.0	0.0	0.0
38	6.124735708787622	0.0	0.0	0.0	0.0
39	6.279378256227807	0.0	0.0	0.0	0.0
40	6.426504424224456	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTGT	55	1.8189894E-12	46.000004	46
CTTAACG	35	1.0198164E-7	46.000004	31
CTGCTCA	55	1.8189894E-12	46.000004	39
ACAACAC	55	1.8189894E-12	46.000004	45
TTCTAAG	55	1.8189894E-12	46.000004	18
CTAGACC	140	0.0	46.000004	46
AGCGTCA	55	1.8189894E-12	46.000004	39
TAATAGG	70	0.0	46.000004	41
CTGTCTC	140	0.0	46.000004	25
GACAGAC	155	0.0	46.000004	37
CCTTAGG	55	1.8189894E-12	46.000004	27
ACAGTGT	35	1.0198164E-7	46.000004	29
GCCTTCG	145	0.0	46.000004	26
ACCGGTT	55	1.8189894E-12	46.000004	43
GCCTTAG	55	1.8189894E-12	46.000004	26
ACTGCAT	55	1.8189894E-12	46.000004	27
TCACCGA	35	1.0198164E-7	46.000004	36
CTCAATT	35	1.0198164E-7	46.000004	19
TAAAGTA	35	1.0198164E-7	46.000004	42
TCCTGTG	70	0.0	46.000004	18
>>END_MODULE