FastQCFastQC Report
Fri 17 Jun 2016
SRR1528114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382214
Sequences flagged as poor quality0
Sequence length59
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAACTTATGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGACAGTT33590.24301591504644No Hit
CTTTACTTATGGGGGGTGGAGTGCAGTGGCTCTATCTCAGCTCACTGCAACCTCTGCCT23160.16755726681975439No Hit
CTTCACTTATGGAGACTTGAGCCACCAGTGCTGAACACCCAGGAGGTTGCTGTCCTTTG21700.1569945030219633No Hit
TTTCACTTATGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTT19350.1399927941693544No Hit
GGCCACTTATGGAGAGTATAAACTTATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG17450.12624673169277695No Hit
AGCGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC17290.12508916853685464No Hit
TAGCACTTATGGGGTTGCTTTTCTTTTGGAATGGCCCTGTGGTCACAGAGCTGAAGGAG17150.12407630077542263No Hit
ACTGACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16960.12270169452776487No Hit
ATTGACTTATGGGCAATCTTACCTCAAGAAACAAGAAAAATTTCAGATAAACAACCTAA16210.11727561723437906No Hit
AATTACTTATGGGAGTATCCTTCAGGATGCCCTCTGAGGGCTGATAGAGATCTGCTAAG15840.11459875243630871No Hit
TAAAACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT15470.11192188763823838No Hit
GACGACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGC15450.11177719224374809No Hit
TAGTACTTATGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA14890.10772572119802No Hit
CAATACTTATGGGCCTTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCA14760.10678520113383311No Hit
TTGGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCC14690.1062787672531171No Hit
GGGCACTTATGGGAAAGTGTTACGACCACTTCATCAGATGAAGTGTTACCATCACTTCA14440.10447007482198849No Hit
AGTTACTTATGGGAAGGAGGCTGCTGTTTGCAACCATTGCACACCAAAGTCCCCATGGG14240.1030231208770856No Hit
GCTAACTTATGGGGAGACCACCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAAT14070.10179321002391815No Hit
CAAAACTTATGGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTACCCAC13930.10078034226248614No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCA150.006696551653.00000429
CTATGAA650.053.00000439
CCTTATT150.006696551653.00000452
ACTATAA307.011513E-753.00000452
CCCCTCA150.006696551653.00000435
GTACCCG150.006696551653.00000452
GTACATT150.006696551653.00000442
CACGGTA650.053.00000451
CGAGTAC150.006696551653.00000426
TCACATC307.011513E-753.00000424
TTAGCGC650.053.00000425
TCATTCA600.053.00000422
CTATCAC150.006696551653.00000422
CGGCATT307.011513E-753.00000435
TTAGAGG150.006696551653.00000444
ATTACTA150.006696551653.00000429
CTGAGAC150.006696551653.00000453
TAGAACC150.006696551653.00000441
TCCCTCA600.053.00000418
GGTCCAC750.053.00000443
Produced by FastQC (version 0.11.5)