##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528114_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1382214
Sequences flagged as poor quality	0
Sequence length	59
%GC	36
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.303149150565687	31.0	30.0	33.0	27.0	34.0
2	31.24276776244489	31.0	31.0	34.0	28.0	34.0
3	31.539037370479534	31.0	31.0	34.0	30.0	34.0
4	35.252021756399515	37.0	35.0	37.0	33.0	37.0
5	34.78532267796448	35.0	35.0	37.0	32.0	37.0
6	35.274246968993225	37.0	35.0	37.0	33.0	37.0
7	35.26043145272729	37.0	35.0	37.0	33.0	37.0
8	35.37834228274348	37.0	35.0	37.0	33.0	37.0
9	36.80629482844191	39.0	37.0	39.0	33.0	39.0
10	37.270010287842545	39.0	37.0	39.0	34.0	39.0
11	37.40824069210701	39.0	37.0	39.0	35.0	39.0
12	37.30781268313011	39.0	37.0	39.0	35.0	39.0
13	37.16129050928438	39.0	37.0	39.0	34.0	39.0
14	37.03373283731752	40.0	36.0	40.0	31.0	40.0
15	36.25721993844658	38.0	35.0	40.0	30.0	40.0
16	35.812067451205095	38.0	34.0	40.0	30.0	40.0
17	35.79632025142272	38.0	34.0	40.0	28.0	40.0
18	35.94691632410032	38.0	35.0	40.0	29.0	40.0
19	36.255000311095095	38.0	35.0	40.0	30.0	40.0
20	36.22210743054259	38.0	35.0	40.0	30.0	40.0
21	36.17031299060782	38.0	35.0	40.0	30.0	40.0
22	36.14459772509901	38.0	35.0	40.0	30.0	40.0
23	36.115398194490865	38.0	35.0	40.0	30.0	40.0
24	36.19557318910096	38.0	35.0	40.0	30.0	40.0
25	36.213155849962455	38.0	35.0	40.0	30.0	40.0
26	35.801198656647955	38.0	34.0	40.0	27.0	40.0
27	35.54986275641833	38.0	34.0	40.0	27.0	40.0
28	35.078610113918685	37.0	34.0	40.0	26.0	40.0
29	34.65115676733125	37.0	33.0	40.0	26.0	40.0
30	34.25399395462642	35.0	33.0	40.0	25.0	40.0
31	34.14052165583622	35.0	33.0	40.0	25.0	40.0
32	34.097660709557275	35.0	33.0	39.0	25.0	40.0
33	33.732304114992324	35.0	33.0	39.0	23.0	40.0
34	33.39978903411483	35.0	33.0	39.0	21.0	40.0
35	33.1550186874102	35.0	32.0	39.0	21.0	40.0
36	32.72558012001036	35.0	31.0	39.0	19.0	40.0
37	32.57196570140369	35.0	31.0	39.0	18.0	40.0
38	32.20183705272845	35.0	31.0	39.0	16.0	40.0
39	31.592773622608366	35.0	30.0	39.0	14.0	40.0
40	30.986032553569853	35.0	28.0	38.0	10.0	40.0
41	30.25652612403	35.0	25.0	38.0	9.0	40.0
42	29.574308319840487	35.0	23.0	38.0	8.0	40.0
43	28.918157390968403	34.0	21.0	38.0	8.0	40.0
44	28.287250020619094	34.0	18.0	38.0	8.0	40.0
45	27.892042766170796	34.0	17.0	38.0	8.0	40.0
46	27.459784809009314	33.0	15.0	37.0	8.0	40.0
47	26.895064006007754	33.0	12.0	37.0	8.0	40.0
48	26.487537385672553	33.0	10.0	36.0	8.0	40.0
49	26.110419225966456	33.0	10.0	36.0	8.0	40.0
50	25.585540299837795	32.0	10.0	35.0	8.0	39.0
51	25.22338147349108	31.0	10.0	35.0	8.0	39.0
52	24.57863181822786	30.0	10.0	35.0	8.0	39.0
53	24.383434113675595	30.0	10.0	35.0	8.0	39.0
54	24.07656412104059	29.0	9.0	35.0	8.0	38.0
55	23.796101761377038	28.0	9.0	35.0	8.0	38.0
56	23.32935710389274	27.0	9.0	35.0	8.0	38.0
57	22.84379987469379	26.0	9.0	35.0	8.0	38.0
58	22.448429114449716	25.0	8.0	35.0	8.0	37.0
59	21.786248005012247	23.0	8.0	34.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	8.0
14	19.0
15	103.0
16	465.0
17	1405.0
18	3392.0
19	6622.0
20	11460.0
21	17453.0
22	25103.0
23	34136.0
24	44854.0
25	57703.0
26	71319.0
27	82993.0
28	81518.0
29	67353.0
30	57700.0
31	61499.0
32	71316.0
33	84412.0
34	101893.0
35	115359.0
36	124535.0
37	133962.0
38	115947.0
39	9685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.58373884217639	24.356286363761328	36.63889962046398	6.421075173598299
2	19.372181152846085	22.751759134258513	36.57776581629184	21.298293896603564
3	20.19499151361511	22.172471122416646	39.135980390880135	18.496556973088104
4	22.219063039442517	22.9360287191419	32.227498780941296	22.617409460474285
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.57394441092335	0.2681205659905051	0.08110176861180686	0.07683325447434333
12	98.89488892458041	0.6317400923446008	0.14809573626081057	0.32527524681416914
13	95.54728862535035	2.9720434028305314	0.7103096915528275	0.7703582802662974
14	47.3268972821864	33.769734643116045	11.05834552392032	7.845022550777231
15	29.02379805153182	44.0542491973023	17.959230625648416	8.962722125517466
16	32.52716294293069	40.68602980435736	15.756749678414486	11.030057574297468
17	18.033604058416426	42.42418323067195	18.79875330448107	20.743459406430553
18	15.925609203784653	50.16444631583822	20.200779329394724	13.709165150982408
19	21.713859069579673	48.14826068901053	19.785648242602086	10.35223199880771
20	16.982753755930702	48.13719149133202	22.208572623341972	12.671482129395303
21	20.40588505108471	47.76337093966636	14.97922897612092	16.851515033128013
22	22.279907452825682	51.042819708091514	15.305878829182745	11.371394009900058
23	13.34663084008699	50.775350271376205	19.116793781570728	16.761225106966073
24	15.692504923260797	55.72335398136613	14.863400312831443	13.720740782541633
25	17.845427697881806	49.609684173362446	18.632570643908974	13.912317484846776
26	15.855504285154106	51.39594881834506	16.294076025854174	16.45447087064666
27	18.006618367343986	53.76699990016017	16.18085187966552	12.045529852830313
28	19.729940515723325	52.01459397748829	14.098757500647514	14.156708006140873
29	19.38346739361633	51.248070125175985	13.193687808110754	16.17477467309693
30	20.946539392597675	51.68483317344492	15.417945412215476	11.95068202174193
31	16.68316194163856	54.421963603320464	13.373544183462185	15.521330271578787
32	16.23692134503051	60.04996331971749	13.809800797850405	9.90331453740159
33	19.452921182971668	57.721235640790795	12.545886526977734	10.27995664925981
34	15.802690466165153	56.83360174329011	14.319707368034182	13.044000422510551
35	20.01745026457553	54.073826484176834	16.10047358802617	9.808249663221469
36	17.14416146848462	54.61035700694683	17.628384606146373	10.617096918422183
37	17.21998185519753	56.27637977910801	15.216963509268464	11.286674856425995
38	14.489941499652007	54.718950900511786	20.295844203574845	10.495263396261361
39	17.293414767901353	56.46759474292693	16.053881671000294	10.185108818171425
40	19.107605624020593	56.83606156499644	13.241364940595307	10.814967870387655
41	21.677540525562613	53.17577451827285	13.209966039990913	11.936718916173616
42	20.098841423976317	50.971774269396775	13.931706667708474	14.99767763891843
43	22.86852831761218	48.44300520758725	16.2795341387079	12.408932336092676
44	20.927584295919445	49.006014987548966	15.91432296301441	14.152077753517183
45	20.36023365412302	51.357459843410645	14.169730591645	14.112575910821334
46	23.44528416005047	46.689586417153926	15.550703436660315	14.314425986135287
47	25.294925387819834	46.85497325305633	13.249106144200537	14.600995214923303
48	25.815756460287627	46.38406209168768	12.981926098274219	14.818255349750473
49	25.19291513470418	48.38519939748838	12.391641236451086	14.03024423135636
50	21.815796974998083	50.90579316950921	12.764376572658067	14.51403328283464
51	20.47244493255024	49.6416618555448	13.796054735373827	16.089838476531128
52	21.15193450507664	48.296790511454816	15.074510893392773	15.476764090075775
53	21.56894663199765	48.117368222286856	14.56395319393379	15.749731951781706
54	18.635536899496024	48.80778229709726	15.53124190610137	17.025438897305335
55	20.023021037263405	46.393177901540575	16.319831806073445	17.26396925512258
56	20.187250310009883	44.54317493528499	16.463731375894035	18.805843378811097
57	19.914065405212217	42.8912599640866	18.63669446265195	18.55798016804923
58	19.199631894916415	41.82304621426204	18.382826393018735	20.5944954978028
59	20.46984041544942	38.703196465959685	19.048208164582327	21.77875495400857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	11.5
4	34.5
5	49.0
6	1515.5
7	2982.0
8	7075.0
9	13934.0
10	16700.0
11	15841.0
12	14982.0
13	12623.0
14	9653.0
15	9042.0
16	9448.0
17	9854.0
18	12172.0
19	19085.5
20	23681.0
21	31156.0
22	38631.0
23	46391.0
24	54151.0
25	61174.5
26	74040.5
27	79883.0
28	81678.5
29	83474.0
30	84285.0
31	82619.0
32	80142.0
33	77647.5
34	75153.0
35	72763.0
36	67045.5
37	63718.0
38	58373.5
39	53029.0
40	56926.5
41	60824.0
42	56846.5
43	53485.0
44	54101.0
45	51981.5
46	49862.0
47	47233.0
48	44227.0
49	43850.0
50	38540.0
51	33230.0
52	31904.5
53	31767.0
54	32955.0
55	31288.5
56	29622.0
57	27313.5
58	22706.5
59	20408.0
60	17051.5
61	13695.0
62	10601.5
63	7508.0
64	7632.5
65	5795.5
66	3834.0
67	3490.0
68	3146.0
69	3034.5
70	1571.0
71	219.0
72	141.0
73	63.0
74	56.0
75	33.5
76	18.0
77	12.5
78	7.0
79	3.5
80	0.0
81	0.0
82	1.0
83	2.0
84	1.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1382214.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.457745298753856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.04429757765344	53.461155517182725
2	3.1693972867098053	3.6421284410066708
3	1.0317411946837254	1.7784456833510855
4	0.5183355297000939	1.1912956337921066
5	0.31825892651766424	0.9143220169453384
6	0.22868362399785122	0.7883787252998719
7	0.17639488693197747	0.7094676739738033
8	0.1384156697238459	0.6362441837079338
9	0.10915725946167078	0.5644737010492802
>10	1.1087816851776995	13.365356335847652
>50	0.07095134773930417	2.726960765530249
>100	0.06243284312517326	8.633239023771152
>500	0.017169023889211592	6.806430799673274
>1k	0.005983144688664645	4.782101498868992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTAACTTATGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGACAGTT	3359	0.24301591504644	No Hit
CTTTACTTATGGGGGGTGGAGTGCAGTGGCTCTATCTCAGCTCACTGCAACCTCTGCCT	2316	0.16755726681975439	No Hit
CTTCACTTATGGAGACTTGAGCCACCAGTGCTGAACACCCAGGAGGTTGCTGTCCTTTG	2170	0.1569945030219633	No Hit
TTTCACTTATGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTT	1935	0.1399927941693544	No Hit
GGCCACTTATGGAGAGTATAAACTTATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	1745	0.12624673169277695	No Hit
AGCGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC	1729	0.12508916853685464	No Hit
TAGCACTTATGGGGTTGCTTTTCTTTTGGAATGGCCCTGTGGTCACAGAGCTGAAGGAG	1715	0.12407630077542263	No Hit
ACTGACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1696	0.12270169452776487	No Hit
ATTGACTTATGGGCAATCTTACCTCAAGAAACAAGAAAAATTTCAGATAAACAACCTAA	1621	0.11727561723437906	No Hit
AATTACTTATGGGAGTATCCTTCAGGATGCCCTCTGAGGGCTGATAGAGATCTGCTAAG	1584	0.11459875243630871	No Hit
TAAAACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1547	0.11192188763823838	No Hit
GACGACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGC	1545	0.11177719224374809	No Hit
TAGTACTTATGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA	1489	0.10772572119802	No Hit
CAATACTTATGGGCCTTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCA	1476	0.10678520113383311	No Hit
TTGGACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCC	1469	0.1062787672531171	No Hit
GGGCACTTATGGGAAAGTGTTACGACCACTTCATCAGATGAAGTGTTACCATCACTTCA	1444	0.10447007482198849	No Hit
AGTTACTTATGGGAAGGAGGCTGCTGTTTGCAACCATTGCACACCAAAGTCCCCATGGG	1424	0.1030231208770856	No Hit
GCTAACTTATGGGGAGACCACCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAAT	1407	0.10179321002391815	No Hit
CAAAACTTATGGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTACCCAC	1393	0.10078034226248614	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.02192135226527875	0.0	0.0	0.0	0.0
12	0.06424475515368822	0.0	0.0	0.0	0.0
13	0.1797840276541838	0.0	0.0	0.0	0.0
14	0.5878250401167981	0.0	0.0	0.0	0.0
15	0.915994194820773	0.0	0.0	0.0	0.0
16	1.1663895749862179	0.0	0.0	0.0	0.0
17	1.3437137809340667	0.0	0.0	0.0	0.0
18	1.5520751490000824	0.0	0.0	0.0	0.0
19	1.71615972635207	0.0	0.0	0.0	0.0
20	1.8858874240891788	0.0	0.0	0.0	0.0
21	2.0546022540648554	0.0	0.0	0.0	0.0
22	2.2270791642972796	0.0	0.0	0.0	0.0
23	2.396300428153672	0.0	0.0	0.0	0.0
24	2.576518541991327	0.0	0.0	0.0	0.0
25	2.7665759426543213	0.0	0.0	0.0	0.0
26	2.9893345024721207	0.0	0.0	0.0	0.0
27	3.2005897784279425	0.0	0.0	0.0	0.0
28	3.4195862579889944	0.0	0.0	0.0	0.0
29	3.674756586172619	0.0	0.0	0.0	0.0
30	3.956478519245211	0.0	0.0	0.0	0.0
31	4.212878758282002	0.0	0.0	0.0	0.0
32	4.405178937559596	0.0	0.0	0.0	0.0
33	4.571506293526183	0.0	0.0	0.0	0.0
34	4.723219414649251	0.0	0.0	0.0	0.0
35	4.871098107818327	0.0	0.0	0.0	0.0
36	5.000745181281625	0.0	0.0	0.0	0.0
37	5.137337633680458	0.0	0.0	0.0	0.0
38	5.266984707143756	0.0	0.0	0.0	0.0
39	5.397934039157468	0.0	0.0	0.0	0.0
40	5.535032925437016	0.0	0.0	0.0	0.0
41	5.686890741954574	0.0	0.0	0.0	0.0
42	5.8295604009219995	0.0	0.0	0.0	0.0
43	5.974038752320553	0.0	0.0	0.0	0.0
44	6.125317787260149	0.0	0.0	0.0	0.0
45	6.275077520557598	0.0	0.0	0.0	0.0
46	6.4181089180112485	0.0	0.0	0.0	0.0
47	6.564902395721647	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCA	15	0.0066965516	53.000004	29
CTATGAA	65	0.0	53.000004	39
CCTTATT	15	0.0066965516	53.000004	52
ACTATAA	30	7.011513E-7	53.000004	52
CCCCTCA	15	0.0066965516	53.000004	35
GTACCCG	15	0.0066965516	53.000004	52
GTACATT	15	0.0066965516	53.000004	42
CACGGTA	65	0.0	53.000004	51
CGAGTAC	15	0.0066965516	53.000004	26
TCACATC	30	7.011513E-7	53.000004	24
TTAGCGC	65	0.0	53.000004	25
TCATTCA	60	0.0	53.000004	22
CTATCAC	15	0.0066965516	53.000004	22
CGGCATT	30	7.011513E-7	53.000004	35
TTAGAGG	15	0.0066965516	53.000004	44
ATTACTA	15	0.0066965516	53.000004	29
CTGAGAC	15	0.0066965516	53.000004	53
TAGAACC	15	0.0066965516	53.000004	41
TCCCTCA	60	0.0	53.000004	18
GGTCCAC	75	0.0	53.000004	43
>>END_MODULE