FastQCFastQC Report
Fri 17 Jun 2016
SRR1528113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175361
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCCTCGCAGGGCAATAAATAATGATGGAGCAAAGAAATCAGTATTGGCCA30161.7198807032350407No Hit
GGGACTCGCAGGGGACAGGATTTACACATCCTCAGGGCTCTCCCATCTTAAA23621.3469357496820844No Hit
GGCTCTCGCAGGGATTCCTTCCCTAGGAAGAGCACTCGGAATAACCTGCTGG19471.110281077320499No Hit
TACCCTCGCAGGGCGTGGTGGTACACACCTATAATTCCAGCTTTTAGGGATG15960.9101225472026276No Hit
ACCACTCGCAGGGTCTGGGGATCAGACTGACACATGTTTACCCCATCTGCGT15630.8913042238582124No Hit
AATTCTCGCAGGGGGGAGGCTGAGGCAGGAGAATCACTTGAACCAGGGAGGC15300.8724859005137973No Hit
GGTCCTCGCAGGGCCGCCCCCCCGAGAACCTGACAGATTCCGAATTGTATTG14740.8405517760505472No Hit
CGGCCTCGCAGGGGCTTTTTTCCGTCTCCGGCCGCCATAGGGAGAGGAGCCG11750.670046361505694No Hit
GTTGCTCGCAGGGATTACCTGTAATAGAAAACCCATTTAAAAACCTAGCTGA11720.6683356048380199No Hit
CGGGCTCGCAGGGCTCTGACATTTCTATTCCAAGCAAGTAGAAATGACAGCC9570.5457313769880419No Hit
CCGACTCGCAGGGTTAACTCAGATGATCATTATATCTACTACTGCGGACAAG9470.5400288547624614No Hit
CGATCTCGCAGGGGGTACTCGCAGGGGGGAATTCTGGACATTAATTAGGGCT8310.4738795969457291No Hit
CACACTCGCAGGGCCCGGGGGGCCACTGGTGTTAAGTCCTAGAGTTCAGAGG8170.46589606582991655No Hit
CTAACTCGCAGGGGACCTTCCTTACAATAAATGTTACAAGAAGTTCTTCAAA7950.45335051693363976No Hit
TGTACTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT7770.4430859769275951No Hit
GATTCTCGCAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA7740.441375220259921No Hit
GGGTCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC6860.39119302467481365No Hit
TATTCTCGCAGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG6560.3740854579980725No Hit
CTATCTCGCAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6450.3678126835499341No Hit
TGGTCTCGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAA6260.35697789132133145No Hit
TCGGCTCGCAGGGTAAAAATTCAAAATAATACAAAATAATAATTATTTTGAA6260.35697789132133145No Hit
TGATCTCGCAGGGAAGCAGTGGGGGGGGGAATTCTGGACATTAATTAGGGCT6080.34671335131528674No Hit
GCTTCTCGCAGGGGAAACTCGCAGGGGGGAATTCTCCAGATTACTTCCATTT5890.335878559086684No Hit
TTATCTCGCAGGGGCCAGCGCAAAAATCGCCAGCGCGAGCATCACTTCCAGC5830.33245704575133583No Hit
TAGACTCGCAGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG5690.3244735146355233No Hit
ACTCCTCGCAGGGGTAAAGAGCTGGCTAAGGACAGAGTGTGGAGTGAACTGG5620.320481749077617No Hit
TTCGCTCGCAGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG5500.3136387224069206No Hit
GCTGCTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT5110.2913988857271571No Hit
AATACTCGCAGGGTCCTTTTTCACCATAGGACTCAAAGCGCTCGAAACGTCC4760.2714400579376258No Hit
TGCTCTCGCAGGGTGATCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTT4710.2685887968248356No Hit
CGTACTCGCAGGGTCTAGAGAATCCTGACTAATACACATGCTTATATAAATA4600.2623160223766972No Hit
GAGGCTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT4330.2469192123676302No Hit
TTTTCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4310.2457787079225141No Hit
GTACCTCGCAGGGCCAAATCAGTAAATTTGTGCTTCGCATGATATTCCCTTA4130.23551416791646942No Hit
TCTGCTCGCAGGGGCTGGGTAGGTAGTTATCAAGAATGACAAATATCCTAAA3730.21270407901414798No Hit
GTAACTCGCAGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACAT3630.20700155678856758No Hit
ACTTCTCGCAGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG3610.2058610523434515No Hit
TAACCTCGCAGGGAAGCAAGTGATATCAACGCAGTTTTTTACTCGCAGGGAG3580.2041502956757774No Hit
AGGCCTCGCAGGGGTAGTTAATACAACAAAATAACAATATGTACAAAAATGT3370.19217499900205862No Hit
CTTCCTCGCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.19160474677950057No Hit
TGTGCTCGCAGGGTTCAAATTGGGGGGGATAACAGAAAAAAAAAAAAAAAAA3250.18533197233136217No Hit
TCTACTCGCAGGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATG3160.18019970232833982No Hit
ATAGCTCGCAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC3130.17848894566066573No Hit
GGTACTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC3060.17449718010275944No Hit
GGCTCTCGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.17335667565764337No Hit
CTTTCTCGCAGGGGATCCAAAGTAAATAAACAACAGAAAAAAAAAAAAAAAA3040.17335667565764337No Hit
CCGACTCGCAGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG3000.17107566676741123No Hit
TATTCTCGCAGGGAAGCAGTGGTATCAACGGGGGGGGAATTCAGATAAAACG2920.16651364898694693No Hit
GGAGCTCGCAGGGATGAGATGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2900.16537314454183083No Hit
AGCTCTCGCAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.16195163120648262No Hit
GCTGCTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780.1585301178711344No Hit
TGGCCTCGCAGGGAGCAGTGATATCAACGCAAAGTGGTACTCGCAGGGAGTC2740.15624910898090225No Hit
GGAGCTCGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.15168709120043797No Hit
TTTTCTCGCAGGGAACCAGTTGATATTCTGGTACCGGCTTGTCACCGTCCGT2600.14826557786508973No Hit
CGATCTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.1340092723011388No Hit
GGCTCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC2280.13001750674323254No Hit
GTGGCTCGCAGGGTGTATCTCTCAATTAAGAAGTCCAATAGACAGTTCCTGG2180.12431498451765215No Hit
GCAACTCGCAGGGGTCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.12260422784997804No Hit
CGGCCTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.12203397562742001No Hit
TAAACTCGCAGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC2130.12146372340486196No Hit
GGCTCTCGCAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2070.11804221006951375No Hit
TGTTCTCGCAGGGATTTTGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.11462069673416553No Hit
CACTCTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.1140504445116075No Hit
GAGTCTCGCAGGGATGAGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.1020751478378887No Hit
TGAGCTCGCAGGGGTTCTATCGTCGTCCAAAAAAAAAAAAAAAAAAAAAAAA1760.10036439117021459No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTCT2950.046.00000410
CGTCATA453.074092E-1046.042
TGATGGA3350.046.024
GATGGGC650.046.018
TGGTCTC2000.046.01
CTTTTAG2100.046.041
ATCCTCA2300.046.028
TAGAAAT1200.046.039
CTCTGAC1250.046.014
TCCTTTT551.8189894E-1246.014
AAACCTA1300.046.041
TTCACTC501.6370905E-1146.01
ATGGGGT501.6370905E-1146.019
TGCACTC405.5642886E-946.01
ACTAATA405.5642886E-946.028
ATGGGCA650.046.019
TTGTTGC206.2967604E-446.039
GGAGACA600.046.037
CATGATA551.8189894E-1246.038
AGGGGAC3450.046.010
Produced by FastQC (version 0.11.5)