FastQCFastQC Report
Fri 17 Jun 2016
SRR1528112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173622
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCCTCGCAGGGCAATAAATAATGATGGAGCAAAGAAATCAGTATTGGCCA29191.6812385527179736No Hit
GGGACTCGCAGGGGACAGGATTTACACATCCTCAGGGCTCTCCCATCTTAAA22631.3034062503599775No Hit
GGCTCTCGCAGGGATTCCTTCCCTAGGAAGAGCACTCGGAATAACCTGCTGG20141.1599912453490917No Hit
TACCCTCGCAGGGCGTGGTGGTACACACCTATAATTCCAGCTTTTAGGGATG15610.8990796097268778No Hit
AATTCTCGCAGGGGGGAGGCTGAGGCAGGAGAATCACTTGAACCAGGGAGGC15210.876041054705049No Hit
GGTCCTCGCAGGGCCGCCCCCCCGAGAACCTGACAGATTCCGAATTGTATTG14720.8478188248033083No Hit
ACCACTCGCAGGGTCTGGGGATCAGACTGACACATGTTTACCCCATCTGCGT14660.8443630415500339No Hit
CGGCCTCGCAGGGGCTTTTTTCCGTCTCCGGCCGCCATAGGGAGAGGAGCCG11710.6744536982640449No Hit
GTTGCTCGCAGGGATTACCTGTAATAGAAAACCCATTTAAAAACCTAGCTGA11130.6410477934823928No Hit
CGGGCTCGCAGGGCTCTGACATTTCTATTCCAAGCAAGTAGAAATGACAGCC9370.5396781513863451No Hit
CCGACTCGCAGGGTTAACTCAGATGATCATTATATCTACTACTGCGGACAAG8930.514335740862333No Hit
CTAACTCGCAGGGGACCTTCCTTACAATAAATGTTACAAGAAGTTCTTCAAA8280.476898088951861No Hit
CGATCTCGCAGGGGGTACTCGCAGGGGGGAATTCTGGACATTAATTAGGGCT7650.4406123647924802No Hit
GATTCTCGCAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA7390.4256373040282913No Hit
CACACTCGCAGGGCCCGGGGGGCCACTGGTGTTAAGTCCTAGAGTTCAGAGG7340.42275748465056273No Hit
TGTACTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT7220.41584591814401406No Hit
GGGTCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC7080.40778242388637387No Hit
TCGGCTCGCAGGGTAAAAATTCAAAATAATACAAAATAATAATTATTTTGAA6430.37034477197590165No Hit
TATTCTCGCAGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG6270.36112934996717005No Hit
CTATCTCGCAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6130.3530658557095299No Hit
TGGTCTCGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAA5990.3450023614518898No Hit
GCTTCTCGCAGGGGAAACTCGCAGGGGGGAATTCTCCAGATTACTTCCATTT5740.33060326456324657No Hit
TTATCTCGCAGGGGCCAGCGCAAAAATCGCCAGCGCGAGCATCACTTCCAGC5730.33002730068770086No Hit
TAGACTCGCAGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG5720.3294513368121551No Hit
TGATCTCGCAGGGAAGCAGTGGGGGGGGGAATTCTGGACATTAATTAGGGCT5600.32253977030560643No Hit
ACTCCTCGCAGGGGTAAAGAGCTGGCTAAGGACAGAGTGTGGAGTGAACTGG5460.3144762760479663No Hit
TTCGCTCGCAGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG5320.3064127817903261No Hit
GCTGCTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT4820.2776145880130398No Hit
AATACTCGCAGGGTCCTTTTTCACCATAGGACTCAAAGCGCTCGAAACGTCC4570.2632154911243967No Hit
TTTTCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4490.2586077801200309No Hit
TGCTCTCGCAGGGTGATCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTT4340.249968321986845No Hit
GAGGCTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT4110.23672115284929326No Hit
GTACCTCGCAGGGCCAAATCAGTAAATTTGTGCTTCGCATGATATTCCCTTA4060.2338413334715647No Hit
CGTACTCGCAGGGTCTAGAGAATCCTGACTAATACACATGCTTATATAAATA3920.2257778392139245No Hit
CTTCCTCGCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3830.22059416433401297No Hit
ACTTCTCGCAGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG3700.21310663395191853No Hit
TCTGCTCGCAGGGGCTGGGTAGGTAGTTATCAAGAATGACAAATATCCTAAA3640.2096508506986442No Hit
TAACCTCGCAGGGAAGCAAGTGATATCAACGCAGTTTTTTACTCGCAGGGAG3590.20677103132091557No Hit
AGGCCTCGCAGGGGTAGTTAATACAACAAAATAACAATATGTACAAAAATGT3560.2050431396942784No Hit
GCTGCTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.1946757899344553No Hit
CCGACTCGCAGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG3290.18949211505454377No Hit
CTTTCTCGCAGGGGATCCAAAGTAAATAAACAACAGAAAAAAAAAAAAAAAA3240.18661229567681514No Hit
TCTACTCGCAGGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATG3190.1837324762990865No Hit
GGAGCTCGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3190.1837324762990865No Hit
GTAACTCGCAGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACAT3140.1808526569213579No Hit
TGTGCTCGCAGGGTTCAAATTGGGGGGGATAACAGAAAAAAAAAAAAAAAAA3100.178548801419175No Hit
GGCTCTCGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.17048530716153482No Hit
ATAGCTCGCAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC2900.16702952390826045No Hit
AGCTCTCGCAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2890.16645356003271475No Hit
TATTCTCGCAGGGAAGCAGTGGTATCAACGGGGGGGGAATTCAGATAAAACG2800.16126988515280322No Hit
GGTACTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC2650.15263042701961732No Hit
TGGCCTCGCAGGGAGCAGTGATATCAACGCAAAGTGGTACTCGCAGGGAGTC2590.14917464376634298No Hit
TTTTCTCGCAGGGAACCAGTTGATATTCTGGTACCGGCTTGTCACCGTCCGT2570.14802271601525152No Hit
GGAGCTCGCAGGGATGAGATGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2510.14456693276197718No Hit
GTGGCTCGCAGGGTGTATCTCTCAATTAAGAAGTCCAATAGACAGTTCCTGG2270.13074379974887976No Hit
CACTCTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.12786398037115113No Hit
CGATCTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.12556012486896823No Hit
GAGTCTCGCAGGGATGAGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.12325626936678531No Hit
GGCTCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC2110.12152837774014814No Hit
GCAACTCGCAGGGGTCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.11519277510914515No Hit
TAAACTCGCAGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC1960.11288891960696225No Hit
CGGCCTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.11116102798032507No Hit
GGAGCTCGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.1025215698471392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA351.0128679E-746.00000442
AGGGGTG4500.046.00000410
ATCCTCA2150.046.00000428
CATGATA351.0128679E-746.00000438
TCCTCAG2150.046.00000429
TTCACAC351.0128679E-746.00000419
TATACTC351.0128679E-746.0000041
GTCATAC351.0128679E-746.00000443
ATCTTAA2250.046.00000445
TAGGAAT700.046.00000445
CACATCC2150.046.00000425
TCTAGAG351.0128679E-746.00000414
GAAATCA2800.046.00000436
AGGATTT2150.046.00000417
TTCGCAT351.0128679E-746.00000434
GACAGGA2250.046.00000414
CTCCCAT2250.046.00000440
AATTCAC351.0128679E-746.00000417
CAGTATT2800.046.00000441
ATGATAT351.0128679E-746.00000439
Produced by FastQC (version 0.11.5)