FastQCFastQC Report
Fri 17 Jun 2016
SRR1528111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160683
Sequences flagged as poor quality0
Sequence length59
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCCTCGCAGGGCAATAAATAATGATGGAGCAAAGAAATCAGTATTGGCCAAATAAAA25041.5583478028167261No Hit
GGGACTCGCAGGGGACAGGATTTACACATCCTCAGGGCTCTCCCATCTTAAAAACATGC19751.2291281591705407No Hit
GGCTCTCGCAGGGATTCCTTCCCTAGGAAGAGCACTCGGAATAACCTGCTGGAAATGAG16951.0548720150856035No Hit
ACCACTCGCAGGGTCTGGGGATCAGACTGACACATGTTTACCCCATCTGCGTCCCGCCC15000.9335150575978791No Hit
TACCCTCGCAGGGCGTGGTGGTACACACCTATAATTCCAGCTTTTAGGGATGCTGAGGC14190.8831052444875935No Hit
AATTCTCGCAGGGGGGAGGCTGAGGCAGGAGAATCACTTGAACCAGGGAGGCAGAGGTT12670.788509051984342No Hit
GTTGCTCGCAGGGATTACCTGTAATAGAAAACCCATTTAAAAACCTAGCTGAACCTACT11600.7219183112090265No Hit
GGTCCTCGCAGGGCCGCCCCCCCGAGAACCTGACAGATTCCGAATTGTATTGAATTATC11010.6852000522768432No Hit
CGGCCTCGCAGGGGCTTTTTTCCGTCTCCGGCCGCCATAGGGAGAGGAGCCGCCGCCAT9760.6074071308103534No Hit
CCGACTCGCAGGGTTAACTCAGATGATCATTATATCTACTACTGCGGACAAGAATCCCT7910.49227360703994816No Hit
CGGGCTCGCAGGGCTCTGACATTTCTATTCCAAGCAAGTAGAAATGACAGCCAAAAAGC7830.48729486006609285No Hit
CTAACTCGCAGGGGACCTTCCTTACAATAAATGTTACAAGAAGTTCTTCAAAGAGAGAG7100.4418637939296628No Hit
GATTCTCGCAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG6450.401411474767088No Hit
CGATCTCGCAGGGGGTACTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCC6380.3970550711649645No Hit
TGTACTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA6040.3758953965260793No Hit
CTATCTCGCAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG5800.36095915560451325No Hit
GGGTCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT5680.3534910351437302No Hit
CACACTCGCAGGGCCCGGGGGGCCACTGGTGTTAAGTCCTAGAGTTCAGAGGCAGGACA5530.3441558845677514No Hit
TTATCTCGCAGGGGCCAGCGCAAAAATCGCCAGCGCGAGCATCACTTCCAGCAAGGTAA5440.33855479422216417No Hit
TATTCTCGCAGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCG5000.3111716858659597No Hit
TGATCTCGCAGGGAAGCAGTGGGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCC4880.3037035654051767No Hit
GCTTCTCGCAGGGGAAACTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCA4690.2918790413422702No Hit
TGGTCTCGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTGTGT4660.29001201122707443No Hit
TAGACTCGCAGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCG4550.28316623413802333No Hit
ACTCCTCGCAGGGGTAAAGAGCTGGCTAAGGACAGAGTGTGGAGTGAACTGGCAGCGGT4370.27196405344684876No Hit
TTCGCTCGCAGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAG4350.2707193667033849No Hit
TCGGCTCGCAGGGTAAAAATTCAAAATAATACAAAATAATAATTATTTTGAATGGCTAA4230.2632512462426019No Hit
GCTGCTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA4220.26262890287087No Hit
AATACTCGCAGGGTCCTTTTTCACCATAGGACTCAAAGCGCTCGAAACGTCCTCTTCCA3880.24146922823198472No Hit
GAGGCTCGCAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA3800.23649048125812935No Hit
GTACCTCGCAGGGCCAAATCAGTAAATTTGTGCTTCGCATGATATTCCCTTACACCCTA3650.22715533068215057No Hit
TCTGCTCGCAGGGGCTGGGTAGGTAGTTATCAAGAATGACAAATATCCTAAAGTTGTTA3570.2221765837082952No Hit
CGTACTCGCAGGGTCTAGAGAATCCTGACTAATACACATGCTTATATAAATAAATGCTT3450.2147084632475122No Hit
TGCTCTCGCAGGGTGATCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCA3340.20786268615846107No Hit
ACTTCTCGCAGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAG3300.2053733126715334No Hit
TTTTCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT3180.19790519221075034No Hit
TAACCTCGCAGGGAAGCAAGTGATATCAACGCAGTTTTTTACTCGCAGGGAGTTCTATC2970.18483598140438007No Hit
AGGCCTCGCAGGGGTAGTTAATACAACAAAATAACAATATGTACAAAAATGTAAAGGAC2880.17923489105879278No Hit
CTTCCTCGCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2870.17861254768706086No Hit
CTTTCTCGCAGGGGATCCAAAGTAAATAAACAACAGAAAAAAAAAAAAAAAAAAAAAAA2680.1667880236241544No Hit
GTAACTCGCAGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGC2640.16429865013722672No Hit
ATAGCTCGCAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA2640.16429865013722672No Hit
CCGACTCGCAGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT2580.1605645899068352No Hit
TGTGCTCGCAGGGTTCAAATTGGGGGGGATAACAGAAAAAAAAAAAAAAAAAAAAAAAA2360.14687303572873295No Hit
TGGCCTCGCAGGGAGCAGTGATATCAACGCAAAGTGGTACTCGCAGGGAGTCTGCTGGT2320.1443836622418053No Hit
GCTGCTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.138782571896218No Hit
TATTCTCGCAGGGAAGCAGTGGTATCAACGGGGGGGGAATTCAGATAAAACGAATAGCT2220.1381602285244861No Hit
GGCTCTCGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190.13629319840929036No Hit
GGTACTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT2140.13318148155063075No Hit
GGAGCTCGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.1282027345767754No Hit
AGCTCTCGCAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.12758039120504347No Hit
TCTACTCGCAGGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTAT2040.12695804783331155No Hit
GGAGCTCGCAGGGATGAGATGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.11202180691174549No Hit
GCAACTCGCAGGGGTCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.10828774668135398No Hit
GGCTCTCGCAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT1740.10828774668135398No Hit
CGATCTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.10704305993789014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTAA2150.053.00000445
CATCTTA2150.053.00000444
TATAATT1950.053.00000430
TTACACA2150.053.00000422
GTACACA1950.053.00000421
TTTACAC2150.053.00000421
GGTACAC1950.053.00000420
TCTTAAA2150.053.00000446
ATTTACA2150.053.00000420
CAGCCAA650.053.048
ACAGCCA650.053.047
ATCCTCA2100.053.028
TCCTCTA401.5861588E-953.026
CTCTGAC550.053.014
ATCCTAA457.6397555E-1153.045
AAACCTA1050.053.041
TTTGAAT503.6379788E-1253.047
TTTGAAA503.6379788E-1253.032
TGCACTC700.053.01
ACTGCGG1150.053.041
Produced by FastQC (version 0.11.5)