FastQCFastQC Report
Fri 17 Jun 2016
SRR1528110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660191
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATAGGACTGGGCATTACTTAATATAAATCTAGCTTACAGGGTAAGCCAGA31550.4778920039806662No Hit
TTAGAGGACTGGGATGATAGACCTGCCTTAACAGAGGGACGCTCAGTGGCGC24620.37292238155321716No Hit
AGTAAGGACTGGGGCGAAACTACGTCTCAAAAAAAAATTATAGGCAAATATT20500.3105161990999574No Hit
GAGGAGGACTGGGTGTTGTTGTTGTTGGAGACAGAGCCTCACTCTGCCACCC20310.30763824408390905No Hit
AATTAGGACTGGGATCGTCCTCAGATCAGGCGAGATCACCCGCTGAATTTAA17580.26628657464279276No Hit
TCCTAGGACTGGGTACCTATATTTGTATGCATCCTTTTTTTTCATTTCTGTC17150.25977330802752535No Hit
GAGGAGGACTGGGGCCGGGAAAAAAACTTTACCTGATGCGTAAGCAACAAAA17150.25977330802752535No Hit
GTCAAGGACTGGGCCCAGTATATTTTTTGAATGCTCAATATAATTAATCATC16770.2540173979954286No Hit
TGTAAGGACTGGGTGGCTCACTGCAACCTCTACCTCCCGGGTTCAAGTGATT16650.2521997421958191No Hit
GTGTAGGACTGAGCGCTCGGCATCACCCAATTTCCCAAACATCTCACGAGAT16320.24720118874689295No Hit
ATCAAGGACTGGGGTTCGCCGTTGTTGGCGGTGCAGTTTGTGCGGGCATATC15970.24189969266469855No Hit
GTTAAGGACTGGGTTAAGAGGTAGAGGGTTGGGCGCAGTGGCTCATGTCTGT15800.23932468028191844No Hit
TGATAGGACTGGGGCTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGT15360.23265994235001689No Hit
GGCTAGGACTGGGGGCACAATCCTGAAGTCAGGAGTTCGAGACCAGCCTGAC14190.2149377983038242No Hit
GCGCAGGACTGGGAAGCTCCAATACTTGGCCACCAGATGAGAAGAGCTGATT14160.2144833843539218No Hit
CTAGAGGACTGGGAGCCCTCTGTATTCATGGTTTCTGCATCTGTGGATTCAA14140.21418044172065356No Hit
ATACAGGACTGGGCCCCCATTAATAAACCCTGTGTGCCAAACTTCACCCTGA14070.2131201425042147No Hit
ATTAAGGACTGGGGGGGCCGAGGTGGGTGGATTACCTGAGGTCAGGAGTTCA13910.21069660143806868No Hit
TGGAAGGACTGGGGCTTTCAACAATTACAATTCTTGTCCCTTTAGCCCAGAA13660.20690981852221554No Hit
TCATAGGACTGGGGGGAATGAGCCACCTCCTGTTGAAGGCCAGCCCCTCTGT13110.19857889610733864No Hit
GCATAGGACTGGGGTGTTATAAACTTGGATTCCTTCGCAAACAATGACAAAG12710.1925200434419736No Hit
ATCTAGGACTGGGCGCTCAGTATGATCCATGAGCGCCGTGAATGACGCATGA12020.18206852259421896No Hit
TAGTAGGACTGGGGAAGATAATGGTGACTTCCTTCAAAAGGTCCCCCTACAG11800.17873615362826817No Hit
CTTTAGGACTGGGGTCCAGGACTGGGTACTGAAAAAAGCTGACCATTCCAGG11680.17691849782865868No Hit
GTCGAGGACTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC10650.16131695221534376No Hit
ATATAGGACTGGGGCAATGGTTTCTGTTATCAGCAAGTAAATCCTGATTGAT10570.16010518168227073No Hit
ACCGAGGACTGGGCTGGTATAATGGTTAGGAGTGAGACTCTGGAGTCAGATA10440.1581360545660271No Hit
TCACAGGACTGGGGACATCTGTGATTGCCAGATCAAGGAAATATTTTAAGTT10360.1569242840329541No Hit
GGCTAGGACTGGGGATGCTATTTTTTTACTCACAACACAACATAACAAACTG10240.1551066282333446No Hit
AGAAAGGACTGGGGGTCTTGTTCCGGGCAAAAGGCCAGCCAAGTCAGGCCCG10140.15359191506700332No Hit
TCATAGGACTGGGCAGAGTGGTCAGCACTGGGTAAGTAGAAAGACTAAACAA8930.13526388575427414No Hit
TTTCAGGACTGGGAACAATTTCATTTACAATCACATCAAAAAAGAATAAAAT8780.13299181600476226No Hit
TTACAGGACTGGGTGCGGGTAACATTTGGGTTACTTGAATACACGTTAGTGA8430.12769031992256788No Hit
ACACAGGACTGGGGAATGGGGAAACCCGGCTGATGAAGAATCAGTCATCCAC8240.12481236490651947No Hit
GCTCAGGACTGGGGTTCCCGGGCTGGTCTCAAACTTTCATGCTCAAGTAATC7980.1208741106740322No Hit
GTCCAGGACTGGGTCAACGCAGAGTTTACAGGACTGGGTCGCATTGGCTCCT7910.11981381145759332No Hit
TGAAAGGACTGGAGGGAGTTGGAGGAGATTGGATTGGGCTTCCCAGGTGGTG7530.11405790142549656No Hit
TGCAAGGACTGGGGTGAAGTTACCCTGGGCCGCAGCAACTCCCTGATGAATG7310.1107255324595458No Hit
ACCTAGGACTGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC7270.11011964719300929No Hit
GTGAAGGACTGGGTCAACGCAGAGTGTGTAGGACTGGGCCAAATCAGTAAAT7170.10860493402666804No Hit
ACTTAGGACTGGGACCTTCTCCTGAATGACTACTGGGTAAATAAAGAAATGA7110.1076961061268633No Hit
AACAAGGACTGGGGACGGGCCGGGCATGGTGGCTCATGCCTGTAATCCCAGC7050.10678727822705854No Hit
GCTGAGGACTGGGGGGAATTCGAGCTCTTGATGATGATACTTAACCAAACCT6830.10345490926110777No Hit
TTTTAGGACTGGGAAGCAGTGGTATCAATGCAAAGTCGTCAGGACTGGGGGC6820.10330343794447364No Hit
CATCAGGACTGGGGCTACTCCTCAGTTAGCCACATAGCACTTGTTATTGCAT6740.10209166741140063No Hit
GGCAAGGACTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6700.10148578214486415No Hit
ACTAAGGACTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6650.10072842556169352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGA551.8189894E-1246.00000420
TCTGCAT1400.046.00000434
TATTTGA351.01868864E-746.00000437
TTTAGCG750.046.00000445
ATCTCTG351.01868864E-746.00000414
GGTACTT351.01868864E-746.00000413
GGTACCT1500.046.00000412
GTCGCAT700.046.00000438
ACACGTT700.046.00000441
TACCCGT551.8189894E-1246.00000438
ACCGGCG1400.046.00000424
TCACCGG351.01868864E-746.00000418
CTACTCC750.046.00000415
TACTTGA700.046.00000432
CTCAAGT1100.046.00000442
TTTACAT700.046.00000430
TCATTTA700.046.00000421
ACTGACC351.01868864E-746.00000444
ACGTTAG700.046.00000443
CCGATAT750.046.00000428
Produced by FastQC (version 0.11.5)