##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528110_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660191
Sequences flagged as poor quality	0
Sequence length	52
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.61510229615369	31.0	31.0	33.0	27.0	34.0
2	31.46767374896053	31.0	31.0	34.0	28.0	34.0
3	31.748004744081637	31.0	31.0	34.0	30.0	34.0
4	35.48567611494249	37.0	35.0	37.0	33.0	37.0
5	35.27740759871007	37.0	35.0	37.0	33.0	37.0
6	35.31799888214168	37.0	35.0	37.0	32.0	37.0
7	35.07953607365141	37.0	35.0	37.0	32.0	37.0
8	34.69335995189271	35.0	35.0	37.0	30.0	37.0
9	37.01359455066791	39.0	37.0	39.0	33.0	39.0
10	37.4272763488142	39.0	37.0	39.0	35.0	39.0
11	37.702358862813945	39.0	37.0	39.0	35.0	39.0
12	37.64866531049348	39.0	37.0	39.0	35.0	39.0
13	37.54721739617777	39.0	37.0	39.0	35.0	39.0
14	37.73803187259445	40.0	38.0	40.0	33.0	40.0
15	36.87423488051185	39.0	36.0	40.0	31.0	40.0
16	36.30615382518089	38.0	35.0	40.0	31.0	40.0
17	35.964994978725855	38.0	35.0	40.0	30.0	40.0
18	36.0353624935814	38.0	35.0	40.0	30.0	40.0
19	36.259826322988346	39.0	35.0	40.0	30.0	40.0
20	36.34257964740507	39.0	35.0	40.0	30.0	40.0
21	36.446714662877866	39.0	35.0	40.0	31.0	40.0
22	36.51822275674767	39.0	35.0	40.0	31.0	40.0
23	36.417903303740886	39.0	35.0	40.0	31.0	40.0
24	36.43004221505595	39.0	35.0	40.0	30.0	40.0
25	36.57982614122277	39.0	35.0	40.0	31.0	40.0
26	36.38630487237784	39.0	35.0	40.0	30.0	40.0
27	36.118633849901016	39.0	35.0	40.0	30.0	40.0
28	35.87453782314512	38.0	35.0	40.0	30.0	40.0
29	35.73234715408117	38.0	35.0	40.0	30.0	40.0
30	35.53555107537061	38.0	35.0	40.0	29.0	40.0
31	35.464176276259444	38.0	34.0	40.0	29.0	40.0
32	35.40504338895865	37.0	34.0	40.0	29.0	40.0
33	35.170971733937606	37.0	34.0	40.0	27.0	40.0
34	34.92330249882231	37.0	34.0	40.0	25.0	40.0
35	34.74991176795806	37.0	33.0	40.0	25.0	40.0
36	34.43013158313276	36.0	33.0	40.0	24.0	40.0
37	34.15295422082397	35.0	33.0	40.0	23.0	40.0
38	33.76318519943471	35.0	33.0	40.0	21.0	40.0
39	33.253029805010975	35.0	33.0	40.0	19.0	40.0
40	32.756541667487134	35.0	33.0	39.0	17.0	40.0
41	32.17230922566348	35.0	31.0	39.0	15.0	40.0
42	31.53556470778911	35.0	30.0	39.0	10.0	40.0
43	30.83775755804002	35.0	28.0	39.0	9.0	40.0
44	30.18638091097879	35.0	25.0	38.0	9.0	40.0
45	29.748966586942263	35.0	23.0	38.0	8.0	40.0
46	29.22130262302879	35.0	22.0	38.0	8.0	40.0
47	28.6040403458999	35.0	20.0	38.0	8.0	40.0
48	28.077536652271842	34.0	18.0	37.0	8.0	40.0
49	27.681654248543225	34.0	15.0	37.0	8.0	40.0
50	27.22660260439782	33.0	14.0	37.0	8.0	40.0
51	26.782214540943453	33.0	12.0	36.0	8.0	40.0
52	25.806618084766377	33.0	10.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	7.0
15	29.0
16	77.0
17	236.0
18	577.0
19	1139.0
20	2015.0
21	3187.0
22	4522.0
23	6509.0
24	8585.0
25	11553.0
26	15228.0
27	19276.0
28	24355.0
29	30679.0
30	36389.0
31	38089.0
32	35821.0
33	36404.0
34	44200.0
35	56347.0
36	65122.0
37	93669.0
38	102543.0
39	23627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.792520649327244	24.497910453187032	35.77616174713075	3.933407150354973
2	25.629855602393853	21.673727754543762	34.34672693205451	18.349689711007873
3	18.592346760255744	23.967912316284227	35.4403498381529	21.99939108530713
4	19.52253211570591	25.454603289048173	33.16176682202575	21.861097773220173
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.3258011696615	0.49000970931139626	0.11481525800866718	0.06937386301842952
12	98.47983386625991	1.2082866927904197	0.17267730096290315	0.1392021399867614
13	95.64338199096927	2.8855285818800924	0.9067073013718757	0.5643821257787519
14	49.72364058280104	28.926174394985694	11.367164956807954	9.983020065405315
15	34.30552673393003	35.647714070625014	21.200682832695385	8.846076362749567
16	39.31135080605461	27.62957992459758	19.740953754292317	13.318115515055492
17	23.155723116492048	32.05738945244634	19.48754224156343	25.29934518949819
18	20.336084557347796	42.83124126199842	21.32428342706883	15.508390753584948
19	24.715726206506904	42.85623402924305	21.754916380259655	10.67312338399039
20	19.46709361381782	40.52690812204347	26.671523846886735	13.334474417251977
21	24.705880570925686	41.41665063595232	15.371612154664332	18.505856638457658
22	24.366130407715342	46.26055187059502	15.19242158708616	14.180896134603472
23	18.575079030159454	45.59635014715438	21.222646173607334	14.605924649078828
24	19.000107544634808	48.18529789106486	15.675009201882487	17.139585362417844
25	23.56636185588716	43.79005469629244	18.072497201567426	14.571086246252978
26	18.47465354723103	45.66648136675598	15.601545613314935	20.25731947269805
27	16.453268826748623	52.77154641611291	15.012473662924819	15.762711094213644
28	16.79347340390887	52.147484591580316	12.342488764615087	18.716553239895728
29	18.990716323003497	50.83498563294562	13.312056662390127	16.862241381660763
30	23.465179016375565	52.700809311244775	12.507441028429653	11.326570643950008
31	17.049611400337174	55.9433557864315	12.046362340595373	14.960670472635949
32	17.36361143971972	58.957634987450604	13.392336460206215	10.286417112623468
33	18.699285509799438	57.46700576045417	12.968519716263929	10.865189013482462
34	17.7230528740925	58.13135895521144	12.200257198295644	11.945330972400411
35	20.138111546506995	56.64936359326316	12.755399573759716	10.457125286470127
36	17.62914065777934	55.48424622571346	16.05035512450185	10.836257992005343
37	16.997353796098402	56.30885607346965	14.484141710504991	12.20964841992696
38	16.772721833529992	55.568615749078674	15.235136498376987	12.423525919014345
39	15.815574583718956	55.64738083372842	15.537170303745432	12.999874278807194
40	18.16444029076434	55.09254140089762	12.948374031151591	13.794644277186451
41	20.081915688035735	54.818529789106485	14.265114186652045	10.834440336205734
42	22.78991988682063	50.66821571333144	14.128790001681333	12.413074398166591
43	23.400652235489428	49.245900050137	14.138332694629282	13.215115019744287
44	19.307291374768816	50.6214110764915	14.86448618657328	15.206811362166404
45	19.608719294870728	47.559115468099385	16.04717422685253	16.784991010177357
46	22.187366989250084	47.821009374559786	16.979177238102306	13.012446398087826
47	24.159523531826395	48.2778468655283	14.073805913743145	13.488823688902151
48	24.211932607381804	47.19285782447807	13.884466767950487	14.71074280018964
49	23.161933440474044	49.29346204356012	12.966550589147685	14.57805392681815
50	21.06087480744209	51.21563305164718	13.264949082916914	14.458543057993822
51	21.668123315828296	50.232129792741794	15.420537389937156	12.67920950149275
52	23.90353700671472	46.46382637751802	14.478688743106161	15.153947872661094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	7.0
8	12.0
9	101.0
10	190.0
11	388.0
12	586.0
13	968.5
14	2477.0
15	3603.0
16	5090.0
17	6577.0
18	6657.5
19	6738.0
20	7209.5
21	7681.0
22	9811.5
23	11942.0
24	15799.5
25	19657.0
26	26439.5
27	33222.0
28	36826.5
29	40431.0
30	41965.5
31	43500.0
32	40047.0
33	36594.0
34	34907.0
35	33220.0
36	29958.5
37	26697.0
38	25779.5
39	23737.0
40	22612.0
41	22115.0
42	21618.0
43	23700.5
44	25783.0
45	26215.5
46	26648.0
47	33897.5
48	41147.0
49	41378.5
50	41610.0
51	41770.0
52	41930.0
53	43617.0
54	45304.0
55	39675.5
56	34047.0
57	30592.5
58	27138.0
59	21133.5
60	15129.0
61	11852.0
62	8575.0
63	7680.0
64	4661.0
65	2537.0
66	1762.5
67	988.0
68	982.5
69	977.0
70	636.0
71	295.0
72	194.5
73	94.0
74	75.5
75	57.0
76	43.5
77	30.0
78	20.5
79	11.0
80	9.0
81	7.0
82	4.5
83	2.0
84	2.0
85	2.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	660191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.44626221043379
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.08974786075544	40.91439527191646
2	5.476778877549718	5.087518156304789
3	1.6908277457739687	2.3559788649868327
4	0.8338197026137764	1.5491123417530153
5	0.5393685141031158	1.2525825717042685
6	0.3812595431911636	1.0624848427972198
7	0.2818606520366813	0.9163961625909678
8	0.21867114520903128	0.8125165878587606
9	0.19539519874470562	0.8167838972000777
>10	1.9489529681892004	19.9036705826213
>50	0.23073844003598304	7.172573772651354
>100	0.0932143904094509	8.25585340757223
>500	0.00951837085166928	2.985498063629045
>1k	0.0098465905362096	6.914635476413716
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGATAGGACTGGGCATTACTTAATATAAATCTAGCTTACAGGGTAAGCCAGA	3155	0.4778920039806662	No Hit
TTAGAGGACTGGGATGATAGACCTGCCTTAACAGAGGGACGCTCAGTGGCGC	2462	0.37292238155321716	No Hit
AGTAAGGACTGGGGCGAAACTACGTCTCAAAAAAAAATTATAGGCAAATATT	2050	0.3105161990999574	No Hit
GAGGAGGACTGGGTGTTGTTGTTGTTGGAGACAGAGCCTCACTCTGCCACCC	2031	0.30763824408390905	No Hit
AATTAGGACTGGGATCGTCCTCAGATCAGGCGAGATCACCCGCTGAATTTAA	1758	0.26628657464279276	No Hit
TCCTAGGACTGGGTACCTATATTTGTATGCATCCTTTTTTTTCATTTCTGTC	1715	0.25977330802752535	No Hit
GAGGAGGACTGGGGCCGGGAAAAAAACTTTACCTGATGCGTAAGCAACAAAA	1715	0.25977330802752535	No Hit
GTCAAGGACTGGGCCCAGTATATTTTTTGAATGCTCAATATAATTAATCATC	1677	0.2540173979954286	No Hit
TGTAAGGACTGGGTGGCTCACTGCAACCTCTACCTCCCGGGTTCAAGTGATT	1665	0.2521997421958191	No Hit
GTGTAGGACTGAGCGCTCGGCATCACCCAATTTCCCAAACATCTCACGAGAT	1632	0.24720118874689295	No Hit
ATCAAGGACTGGGGTTCGCCGTTGTTGGCGGTGCAGTTTGTGCGGGCATATC	1597	0.24189969266469855	No Hit
GTTAAGGACTGGGTTAAGAGGTAGAGGGTTGGGCGCAGTGGCTCATGTCTGT	1580	0.23932468028191844	No Hit
TGATAGGACTGGGGCTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGT	1536	0.23265994235001689	No Hit
GGCTAGGACTGGGGGCACAATCCTGAAGTCAGGAGTTCGAGACCAGCCTGAC	1419	0.2149377983038242	No Hit
GCGCAGGACTGGGAAGCTCCAATACTTGGCCACCAGATGAGAAGAGCTGATT	1416	0.2144833843539218	No Hit
CTAGAGGACTGGGAGCCCTCTGTATTCATGGTTTCTGCATCTGTGGATTCAA	1414	0.21418044172065356	No Hit
ATACAGGACTGGGCCCCCATTAATAAACCCTGTGTGCCAAACTTCACCCTGA	1407	0.2131201425042147	No Hit
ATTAAGGACTGGGGGGGCCGAGGTGGGTGGATTACCTGAGGTCAGGAGTTCA	1391	0.21069660143806868	No Hit
TGGAAGGACTGGGGCTTTCAACAATTACAATTCTTGTCCCTTTAGCCCAGAA	1366	0.20690981852221554	No Hit
TCATAGGACTGGGGGGAATGAGCCACCTCCTGTTGAAGGCCAGCCCCTCTGT	1311	0.19857889610733864	No Hit
GCATAGGACTGGGGTGTTATAAACTTGGATTCCTTCGCAAACAATGACAAAG	1271	0.1925200434419736	No Hit
ATCTAGGACTGGGCGCTCAGTATGATCCATGAGCGCCGTGAATGACGCATGA	1202	0.18206852259421896	No Hit
TAGTAGGACTGGGGAAGATAATGGTGACTTCCTTCAAAAGGTCCCCCTACAG	1180	0.17873615362826817	No Hit
CTTTAGGACTGGGGTCCAGGACTGGGTACTGAAAAAAGCTGACCATTCCAGG	1168	0.17691849782865868	No Hit
GTCGAGGACTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC	1065	0.16131695221534376	No Hit
ATATAGGACTGGGGCAATGGTTTCTGTTATCAGCAAGTAAATCCTGATTGAT	1057	0.16010518168227073	No Hit
ACCGAGGACTGGGCTGGTATAATGGTTAGGAGTGAGACTCTGGAGTCAGATA	1044	0.1581360545660271	No Hit
TCACAGGACTGGGGACATCTGTGATTGCCAGATCAAGGAAATATTTTAAGTT	1036	0.1569242840329541	No Hit
GGCTAGGACTGGGGATGCTATTTTTTTACTCACAACACAACATAACAAACTG	1024	0.1551066282333446	No Hit
AGAAAGGACTGGGGGTCTTGTTCCGGGCAAAAGGCCAGCCAAGTCAGGCCCG	1014	0.15359191506700332	No Hit
TCATAGGACTGGGCAGAGTGGTCAGCACTGGGTAAGTAGAAAGACTAAACAA	893	0.13526388575427414	No Hit
TTTCAGGACTGGGAACAATTTCATTTACAATCACATCAAAAAAGAATAAAAT	878	0.13299181600476226	No Hit
TTACAGGACTGGGTGCGGGTAACATTTGGGTTACTTGAATACACGTTAGTGA	843	0.12769031992256788	No Hit
ACACAGGACTGGGGAATGGGGAAACCCGGCTGATGAAGAATCAGTCATCCAC	824	0.12481236490651947	No Hit
GCTCAGGACTGGGGTTCCCGGGCTGGTCTCAAACTTTCATGCTCAAGTAATC	798	0.1208741106740322	No Hit
GTCCAGGACTGGGTCAACGCAGAGTTTACAGGACTGGGTCGCATTGGCTCCT	791	0.11981381145759332	No Hit
TGAAAGGACTGGAGGGAGTTGGAGGAGATTGGATTGGGCTTCCCAGGTGGTG	753	0.11405790142549656	No Hit
TGCAAGGACTGGGGTGAAGTTACCCTGGGCCGCAGCAACTCCCTGATGAATG	731	0.1107255324595458	No Hit
ACCTAGGACTGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC	727	0.11011964719300929	No Hit
GTGAAGGACTGGGTCAACGCAGAGTGTGTAGGACTGGGCCAAATCAGTAAAT	717	0.10860493402666804	No Hit
ACTTAGGACTGGGACCTTCTCCTGAATGACTACTGGGTAAATAAAGAAATGA	711	0.1076961061268633	No Hit
AACAAGGACTGGGGACGGGCCGGGCATGGTGGCTCATGCCTGTAATCCCAGC	705	0.10678727822705854	No Hit
GCTGAGGACTGGGGGGAATTCGAGCTCTTGATGATGATACTTAACCAAACCT	683	0.10345490926110777	No Hit
TTTTAGGACTGGGAAGCAGTGGTATCAATGCAAAGTCGTCAGGACTGGGGGC	682	0.10330343794447364	No Hit
CATCAGGACTGGGGCTACTCCTCAGTTAGCCACATAGCACTTGTTATTGCAT	674	0.10209166741140063	No Hit
GGCAAGGACTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA	670	0.10148578214486415	No Hit
ACTAAGGACTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA	665	0.10072842556169352	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.03983695627477503	0.0	0.0	0.0	0.0
12	0.08679306443135396	0.0	0.0	0.0	0.0
13	0.11935939750769095	0.0	0.0	0.0	0.0
14	0.5625644699791424	0.0	0.0	0.0	0.0
15	0.9610855040435268	0.0	0.0	0.0	0.0
16	1.1884439503113493	0.0	0.0	0.0	0.0
17	1.36066683732435	0.0	0.0	0.0	0.0
18	1.6080194973878772	0.0	0.0	0.0	0.0
19	1.810536647727703	0.0	0.0	0.0	0.0
20	2.038652450578696	0.0	0.0	0.0	0.0
21	2.278583016127151	0.0	0.0	0.0	0.0
22	2.523512135124532	0.0	0.0	0.0	0.0
23	2.731785195496455	0.0	0.0	0.0	0.0
24	2.9570230433313998	0.0	0.0	0.0	0.0
25	3.2078595436775115	0.0	0.0	0.0	0.0
26	3.5065609800800073	0.0	0.0	0.0	0.0
27	3.817531593129867	0.0	0.0	0.0	0.0
28	4.125169837213776	0.0	0.0	0.0	0.0
29	4.4893068824022135	0.0	0.0	0.0	0.0
30	4.957959136068198	0.0	0.0	0.0	0.0
31	5.352390444583461	0.0	0.0	0.0	0.0
32	5.693200907010244	0.0	0.0	0.0	0.0
33	5.937978554690991	0.0	0.0	0.0	0.0
34	6.1851797434378835	0.0	0.0	0.0	0.0
35	6.400420484374976	0.0	0.0	0.0	0.0
36	6.612025913712849	0.0	0.0	0.0	0.0
37	6.81984456013487	0.0	0.0	0.0	0.0
38	7.030238218939671	0.0	0.0	0.0	0.0
39	7.24350983276052	0.0	0.0	0.0	0.0
40	7.458144688431076	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGA	55	1.8189894E-12	46.000004	20
TCTGCAT	140	0.0	46.000004	34
TATTTGA	35	1.01868864E-7	46.000004	37
TTTAGCG	75	0.0	46.000004	45
ATCTCTG	35	1.01868864E-7	46.000004	14
GGTACTT	35	1.01868864E-7	46.000004	13
GGTACCT	150	0.0	46.000004	12
GTCGCAT	70	0.0	46.000004	38
ACACGTT	70	0.0	46.000004	41
TACCCGT	55	1.8189894E-12	46.000004	38
ACCGGCG	140	0.0	46.000004	24
TCACCGG	35	1.01868864E-7	46.000004	18
CTACTCC	75	0.0	46.000004	15
TACTTGA	70	0.0	46.000004	32
CTCAAGT	110	0.0	46.000004	42
TTTACAT	70	0.0	46.000004	30
TCATTTA	70	0.0	46.000004	21
ACTGACC	35	1.01868864E-7	46.000004	44
ACGTTAG	70	0.0	46.000004	43
CCGATAT	75	0.0	46.000004	28
>>END_MODULE