FastQCFastQC Report
Fri 17 Jun 2016
SRR1528109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657569
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATAGGACTGGGCATTACTTAATATAAATCTAGCTTACAGGGTAAGCCAGA31530.47949340677556274No Hit
TTAGAGGACTGGGATGATAGACCTGCCTTAACAGAGGGACGCTCAGTGGCGC22580.34338601728487805No Hit
AGTAAGGACTGGGGCGAAACTACGTCTCAAAAAAAAATTATAGGCAAATATT20010.3043026663361563No Hit
GAGGAGGACTGGGTGTTGTTGTTGTTGGAGACAGAGCCTCACTCTGCCACCC19940.30323813926751414No Hit
AATTAGGACTGGGATCGTCCTCAGATCAGGCGAGATCACCCGCTGAATTTAA17700.2691732730709629No Hit
TCCTAGGACTGGGTACCTATATTTGTATGCATCCTTTTTTTTCATTTCTGTC16700.2539657435189311No Hit
GTCAAGGACTGGGCCCAGTATATTTTTTGAATGCTCAATATAATTAATCATC16590.2522929152682076No Hit
TGATAGGACTGGGGCTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGT16430.2498597105398825No Hit
GAGGAGGACTGGGGCCGGGAAAAAAACTTTACCTGATGCGTAAGCAACAAAA16380.2490993340622809No Hit
TGTAAGGACTGGGTGGCTCACTGCAACCTCTACCTCCCGGGTTCAAGTGATT16180.24605782815187455No Hit
GTTAAGGACTGGGTTAAGAGGTAGAGGGTTGGGCGCAGTGGCTCATGTCTGT15370.2337397292147288No Hit
GTGTAGGACTGAGCGCTCGGCATCACCCAATTTCCCAAACATCTCACGAGAT15350.2334355786236882No Hit
ATCAAGGACTGGGGTTCGCCGTTGTTGGCGGTGCAGTTTGTGCGGGCATATC15130.2300899221222412No Hit
GCGCAGGACTGGGAAGCTCCAATACTTGGCCACCAGATGAGAAGAGCTGATT15110.22978577153120053No Hit
CTAGAGGACTGGGAGCCCTCTGTATTCATGGTTTCTGCATCTGTGGATTCAA14800.22507143737007068No Hit
GGCTAGGACTGGGGGCACAATCCTGAAGTCAGGAGTTCGAGACCAGCCTGAC14260.2168593714119735No Hit
ATTAAGGACTGGGGGGGCCGAGGTGGGTGGATTACCTGAGGTCAGGAGTTCA13940.21199296195532333No Hit
ATACAGGACTGGGCCCCCATTAATAAACCCTGTGTGCCAAACTTCACCCTGA13300.20226014304202294No Hit
TAGTAGGACTGGGGAAGATAATGGTGACTTCCTTCAAAAGGTCCCCCTACAG12350.18781298996759277No Hit
TCATAGGACTGGGGGGAATGAGCCACCTCCTGTTGAAGGCCAGCCCCTCTGT12300.18705261348999117No Hit
TGGAAGGACTGGGGCTTTCAACAATTACAATTCTTGTCCCTTTAGCCCAGAA12180.18522770994374735No Hit
GCATAGGACTGGGGTGTTATAAACTTGGATTCCTTCGCAAACAATGACAAAG11920.18127375226021908No Hit
CTTTAGGACTGGGGTCCAGGACTGGGTACTGAAAAAAGCTGACCATTCCAGG11760.178840547531894No Hit
ATCTAGGACTGGGCGCTCAGTATGATCCATGAGCGCCGTGAATGACGCATGA11330.17230130982452033No Hit
ACCGAGGACTGGGCTGGTATAATGGTTAGGAGTGAGACTCTGGAGTCAGATA10590.1610477379560168No Hit
TCACAGGACTGGGGACATCTGTGATTGCCAGATCAAGGAAATATTTTAAGTT10410.15831038263665106No Hit
ATATAGGACTGGGGCAATGGTTTCTGTTATCAGCAAGTAAATCCTGATTGAT10220.155420952021765No Hit
GTCGAGGACTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC9770.1485775637233507No Hit
GGCTAGGACTGGGGATGCTATTTTTTTACTCACAACACAACATAACAAACTG9190.13975719658317226No Hit
AGAAAGGACTGGGGGTCTTGTTCCGGGCAAAAGGCCAGCCAAGTCAGGCCCG8880.1350428624220424No Hit
TTTCAGGACTGGGAACAATTTCATTTACAATCACATCAAAAAAGAATAAAAT8430.1281994741236281No Hit
TCATAGGACTGGGCAGAGTGGTCAGCACTGGGTAAGTAGAAAGACTAAACAA8410.12789532353258745No Hit
GTCCAGGACTGGGTCAACGCAGAGTTTACAGGACTGGGTCGCATTGGCTCCT8170.12424551644009982No Hit
TTACAGGACTGGGTGCGGGTAACATTTGGGTTACTTGAATACACGTTAGTGA8070.12272476348489665No Hit
TTTTAGGACTGGGAAGCAGTGGTATCAATGCAAAGTCGTCAGGACTGGGGGC7920.12044363405209187No Hit
ACACAGGACTGGGGAATGGGGAAACCCGGCTGATGAAGAATCAGTCATCCAC7910.12029155875657156No Hit
GCTCAGGACTGGGGTTCCCGGGCTGGTCTCAAACTTTCATGCTCAAGTAATC7730.11755420343720582No Hit
ACCTAGGACTGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC7610.115729299890962No Hit
TGCAAGGACTGGGGTGAAGTTACCCTGGGCCGCAGCAACTCCCTGATGAATG7460.11344817045815725No Hit
ACTTAGGACTGGGACCTTCTCCTGAATGACTACTGGGTAAATAAAGAAATGA7460.11344817045815725No Hit
GTGAAGGACTGGGTCAACGCAGAGTGTGTAGGACTGGGCCAAATCAGTAAAT7190.10934213747910866No Hit
GATCAGGACTGGGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGT7000.10645270686422262No Hit
AACAAGGACTGGGGACGGGCCGGGCATGGTGGCTCATGCCTGTAATCCCAGC6930.10538817979558039No Hit
TGAAAGGACTGGAGGGAGTTGGAGGAGATTGGATTGGGCTTCCCAGGTGGTG6920.10523610450006006No Hit
CATCAGGACTGGGGCTACTCCTCAGTTAGCCACATAGCACTTGTTATTGCAT6850.10417157743141785No Hit
GGCAAGGACTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6820.10371535154485689No Hit
GCTGAGGACTGGGGGGAATTCGAGCTCTTGATGATGATACTTAACCAAACCT6770.1029549750672553No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACTG1100.046.00000446
TCGCAGT1050.046.00000445
ATTAGCC551.8189894E-1246.00000446
TTTACTC1050.046.00000425
TTAGCTG551.8189894E-1246.00000428
ACGTTAT551.8189894E-1246.00000416
TCACAAC1050.046.00000430
ATCAGTC551.8189894E-1246.00000440
GCTATTT1050.046.00000417
TGGGTCC1050.046.00000410
TAGAAAT551.8189894E-1246.00000441
TTGTCCC1100.046.00000434
TTACTCA1050.046.00000426
TTGTAGT551.8189894E-1246.00000422
CAACATA1050.046.00000438
CATTAGC551.8189894E-1246.00000445
CGCTCCA551.8189894E-1246.00000439
ATTCATT551.8189894E-1246.00000423
TTCTTGT1100.046.00000431
CTCATTA551.8189894E-1246.00000443
Produced by FastQC (version 0.11.5)