##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1528109_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 657569 Sequences flagged as poor quality 0 Sequence length 52 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.639873534184247 31.0 31.0 33.0 27.0 34.0 2 31.48368916417897 31.0 31.0 34.0 28.0 34.0 3 31.759506606911213 31.0 31.0 34.0 30.0 34.0 4 35.500259288378864 37.0 35.0 37.0 33.0 37.0 5 35.40377967939486 37.0 35.0 37.0 33.0 37.0 6 35.36159247166457 37.0 35.0 37.0 33.0 37.0 7 35.06434914054647 37.0 35.0 37.0 32.0 37.0 8 34.66828424089335 35.0 35.0 37.0 30.0 37.0 9 36.98913878239394 39.0 37.0 39.0 33.0 39.0 10 37.39277551101101 39.0 37.0 39.0 34.0 39.0 11 37.68084261879742 39.0 37.0 39.0 35.0 39.0 12 37.65782298131451 39.0 37.0 39.0 35.0 39.0 13 37.538393689483534 39.0 37.0 39.0 35.0 39.0 14 37.695604567733575 40.0 37.0 40.0 33.0 40.0 15 36.850759387988184 39.0 36.0 40.0 31.0 40.0 16 36.27813963249484 38.0 35.0 40.0 31.0 40.0 17 35.966374631407504 38.0 35.0 40.0 30.0 40.0 18 35.94573345154653 38.0 35.0 40.0 29.0 40.0 19 36.25820712351099 39.0 35.0 40.0 30.0 40.0 20 36.34040229998677 39.0 35.0 40.0 30.0 40.0 21 36.49972398333863 39.0 35.0 40.0 31.0 40.0 22 36.453499176512274 39.0 35.0 40.0 31.0 40.0 23 36.44688390115714 39.0 35.0 40.0 31.0 40.0 24 36.54409651306555 39.0 35.0 40.0 31.0 40.0 25 36.613813911543886 39.0 35.0 40.0 31.0 40.0 26 36.291681937560924 39.0 35.0 40.0 30.0 40.0 27 35.995357141227764 39.0 35.0 40.0 30.0 40.0 28 35.800743344044506 38.0 35.0 40.0 29.0 40.0 29 35.65562853480015 38.0 35.0 40.0 29.0 40.0 30 35.34417072580976 38.0 34.0 40.0 28.0 40.0 31 35.33701862466144 38.0 34.0 40.0 28.0 40.0 32 35.35625614954476 37.0 34.0 40.0 28.0 40.0 33 35.220004288523334 37.0 34.0 40.0 27.0 40.0 34 35.03026906682037 37.0 34.0 40.0 26.0 40.0 35 34.68074681136124 37.0 33.0 40.0 25.0 40.0 36 34.40704017373082 36.0 33.0 40.0 24.0 40.0 37 34.12970197804337 35.0 33.0 40.0 23.0 40.0 38 33.805048595660686 35.0 33.0 40.0 21.0 40.0 39 33.310522545922936 35.0 33.0 40.0 20.0 40.0 40 32.742814822474905 35.0 33.0 39.0 17.0 40.0 41 32.137907960989644 35.0 31.0 39.0 15.0 40.0 42 31.505080075246855 35.0 30.0 39.0 10.0 40.0 43 30.843985954325706 35.0 28.0 39.0 9.0 40.0 44 30.08952976797872 35.0 24.0 38.0 9.0 40.0 45 29.663249027858672 35.0 23.0 38.0 8.0 40.0 46 29.13502461338658 35.0 22.0 38.0 8.0 40.0 47 28.632619542587925 35.0 20.0 38.0 8.0 40.0 48 28.092746160478978 34.0 18.0 37.0 8.0 40.0 49 27.561944069747813 33.0 15.0 37.0 8.0 40.0 50 27.085578851801102 33.0 13.0 36.0 8.0 40.0 51 26.654938112958487 33.0 11.0 36.0 8.0 40.0 52 25.528069297670662 32.0 10.0 35.0 8.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 7.0 15 25.0 16 81.0 17 232.0 18 586.0 19 1164.0 20 2020.0 21 3195.0 22 4573.0 23 6256.0 24 8580.0 25 11663.0 26 15229.0 27 19501.0 28 24899.0 29 30767.0 30 36260.0 31 38260.0 32 35504.0 33 36229.0 34 44205.0 35 56346.0 36 64628.0 37 93752.0 38 102994.0 39 20610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.780275530020425 24.47879994342799 35.776777798223456 3.96414672832813 2 25.614802400964763 21.52869128562934 34.466344976724876 18.390161336681018 3 18.709367381978165 23.719183842304002 35.48023097195884 22.091217803758997 4 19.43354993924592 25.360228356263754 33.330950820370184 21.875270884120145 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 0.0 100.0 0.0 0.0 9 0.0 0.0 0.0 100.0 10 0.0 0.0 100.0 0.0 11 99.33223737737028 0.48998660216646467 0.11040666454775087 0.06736935591550088 12 98.49582325200853 1.1956159733807403 0.1660662227081873 0.14249455190253799 13 95.64958202105026 2.861144609919263 0.9354151427454761 0.5538582262849983 14 49.481955505810035 29.081358762350412 11.349987605863415 10.086698125976133 15 34.33860172848781 35.73465294136433 21.051631083582105 8.87511424656576 16 39.414418867069465 27.56714504485461 19.626837639852244 13.391598448223686 17 23.140385267553672 32.09670772192728 19.426858626243025 25.336048384276022 18 20.383564310361347 42.90439482396524 21.18454489186686 15.527495973806552 19 24.864158742276476 42.77254554274912 21.637729272517408 10.725566442456989 20 19.493315530385406 40.46784443913871 26.67780871665179 13.361031313824101 21 24.67877895703721 41.458006688271496 15.359300696961078 18.503913657730216 22 24.424509062927235 46.15500426571204 15.262276658419117 14.158210012941607 23 18.524595897920978 45.43553604260541 21.44809137900357 14.591776680470034 24 19.081495630116383 48.39978770288745 15.535251813878087 16.983464853118075 25 23.548403285434684 43.64606604021783 18.159158962785654 14.646371711561828 26 18.483991794017054 45.533928758807065 15.695843325947543 20.28623612122834 27 16.397062513591727 52.83521577203305 15.03933427518633 15.72838743918889 28 16.73345306728267 52.37032159362743 12.252250334185462 18.64397500490443 29 19.043020580349744 50.70144730058747 13.305067605072624 16.950464513990166 30 23.533956132360252 52.51585765144038 12.55290319342913 11.397283022770234 31 16.940427544485825 55.89527486849288 11.94992464669107 15.214372940330218 32 17.390570419225966 59.028330106802485 13.389621469381922 10.191478004589632 33 18.840456286716677 57.39276030348146 12.861463968039855 10.905319441762005 34 17.761482065000024 58.24803176548773 12.067630925423796 11.922855244088453 35 20.02253755879611 56.63238382587987 12.89720166248713 10.44787695283689 36 17.52622158283009 55.39905317921009 16.303384131551212 10.771341106408606 37 17.002018039171553 56.25797444830884 14.512697526799471 12.22730998572013 38 16.726609678984257 55.59614276220442 15.212852187375015 12.464395371436305 39 15.797581698650637 55.5652714772138 15.59410495324445 13.043041870891114 40 18.15094689682756 55.00259288378862 13.013691338855693 13.832768880528127 41 20.123667630317122 54.83972024228636 14.171592638947395 10.865019488449121 42 22.860262573205244 50.63742360117342 14.158818314123689 12.343495511497652 43 23.525744066402158 49.287907428726115 13.95959967699207 13.226748827879659 44 19.234483377409823 50.7166548301395 14.9096140481075 15.139247744343178 45 19.562357714551627 47.531893991352995 16.067819498790243 16.83792879530513 46 22.1114438180632 47.74616808274112 17.065585512699048 13.076802586496628 47 24.276387725090448 48.304588567891734 13.931769897911856 13.487253809105965 48 24.303609202988582 47.17497327276681 13.786385915394431 14.735031608850175 49 23.041232174874423 49.22677315992694 13.137480629409234 14.5945140357894 50 21.056345417743234 51.24633308443677 13.316625327532167 14.380696170287832 51 21.63362323953836 50.321411137082194 15.466361705007383 12.578603918372064 52 24.093441144579504 46.26784413498812 14.577329527395605 15.061385193036777 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.5 6 3.0 7 8.0 8 13.0 9 102.0 10 191.0 11 404.0 12 617.0 13 988.5 14 2385.0 15 3410.0 16 4880.0 17 6350.0 18 6521.5 19 6693.0 20 7121.0 21 7549.0 22 9713.5 23 11878.0 24 15595.0 25 19312.0 26 25996.0 27 32680.0 28 36528.0 29 40376.0 30 41888.0 31 43400.0 32 40060.0 33 36720.0 34 34915.0 35 33110.0 36 29787.5 37 26465.0 38 25655.0 39 23846.5 40 22848.0 41 22216.5 42 21585.0 43 23617.5 44 25650.0 45 26147.0 46 26644.0 47 34296.5 48 41949.0 49 41909.5 50 41870.0 51 41789.0 52 41708.0 53 43290.5 54 44873.0 55 39131.0 56 33389.0 57 30056.5 58 26724.0 59 20970.0 60 15216.0 61 11887.5 62 8559.0 63 7602.5 64 4609.0 65 2572.0 66 1770.0 67 968.0 68 961.5 69 955.0 70 599.5 71 244.0 72 174.5 73 105.0 74 75.5 75 46.0 76 36.5 77 27.0 78 19.0 79 11.0 80 6.5 81 2.0 82 2.5 83 3.0 84 3.0 85 3.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 657569.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.496543256405666 #Duplication Level Percentage of deduplicated Percentage of total 1 88.14137891844082 40.982694375605256 2 5.411679957945222 5.032488225088473 3 1.7032259983805011 2.3758236392740106 4 0.8373188556232574 1.5572972955956352 5 0.5132066117560518 1.193116671149432 6 0.3776093984780125 1.053451903821494 7 0.290294086777858 0.9448370094061819 8 0.2256691926015154 0.8394269900347603 9 0.19285366982960706 0.8070326101250012 >10 1.964643604608029 20.046384964146576 >50 0.23248288918191873 7.240469868434614 >100 0.09015316527404214 8.133801472714259 >500 0.010567403988166111 3.4308357107644913 >1k 0.008916247115015156 6.362339263839843 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGATAGGACTGGGCATTACTTAATATAAATCTAGCTTACAGGGTAAGCCAGA 3153 0.47949340677556274 No Hit TTAGAGGACTGGGATGATAGACCTGCCTTAACAGAGGGACGCTCAGTGGCGC 2258 0.34338601728487805 No Hit AGTAAGGACTGGGGCGAAACTACGTCTCAAAAAAAAATTATAGGCAAATATT 2001 0.3043026663361563 No Hit GAGGAGGACTGGGTGTTGTTGTTGTTGGAGACAGAGCCTCACTCTGCCACCC 1994 0.30323813926751414 No Hit AATTAGGACTGGGATCGTCCTCAGATCAGGCGAGATCACCCGCTGAATTTAA 1770 0.2691732730709629 No Hit TCCTAGGACTGGGTACCTATATTTGTATGCATCCTTTTTTTTCATTTCTGTC 1670 0.2539657435189311 No Hit GTCAAGGACTGGGCCCAGTATATTTTTTGAATGCTCAATATAATTAATCATC 1659 0.2522929152682076 No Hit TGATAGGACTGGGGCTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGT 1643 0.2498597105398825 No Hit GAGGAGGACTGGGGCCGGGAAAAAAACTTTACCTGATGCGTAAGCAACAAAA 1638 0.2490993340622809 No Hit TGTAAGGACTGGGTGGCTCACTGCAACCTCTACCTCCCGGGTTCAAGTGATT 1618 0.24605782815187455 No Hit GTTAAGGACTGGGTTAAGAGGTAGAGGGTTGGGCGCAGTGGCTCATGTCTGT 1537 0.2337397292147288 No Hit GTGTAGGACTGAGCGCTCGGCATCACCCAATTTCCCAAACATCTCACGAGAT 1535 0.2334355786236882 No Hit ATCAAGGACTGGGGTTCGCCGTTGTTGGCGGTGCAGTTTGTGCGGGCATATC 1513 0.2300899221222412 No Hit GCGCAGGACTGGGAAGCTCCAATACTTGGCCACCAGATGAGAAGAGCTGATT 1511 0.22978577153120053 No Hit CTAGAGGACTGGGAGCCCTCTGTATTCATGGTTTCTGCATCTGTGGATTCAA 1480 0.22507143737007068 No Hit GGCTAGGACTGGGGGCACAATCCTGAAGTCAGGAGTTCGAGACCAGCCTGAC 1426 0.2168593714119735 No Hit ATTAAGGACTGGGGGGGCCGAGGTGGGTGGATTACCTGAGGTCAGGAGTTCA 1394 0.21199296195532333 No Hit ATACAGGACTGGGCCCCCATTAATAAACCCTGTGTGCCAAACTTCACCCTGA 1330 0.20226014304202294 No Hit TAGTAGGACTGGGGAAGATAATGGTGACTTCCTTCAAAAGGTCCCCCTACAG 1235 0.18781298996759277 No Hit TCATAGGACTGGGGGGAATGAGCCACCTCCTGTTGAAGGCCAGCCCCTCTGT 1230 0.18705261348999117 No Hit TGGAAGGACTGGGGCTTTCAACAATTACAATTCTTGTCCCTTTAGCCCAGAA 1218 0.18522770994374735 No Hit GCATAGGACTGGGGTGTTATAAACTTGGATTCCTTCGCAAACAATGACAAAG 1192 0.18127375226021908 No Hit CTTTAGGACTGGGGTCCAGGACTGGGTACTGAAAAAAGCTGACCATTCCAGG 1176 0.178840547531894 No Hit ATCTAGGACTGGGCGCTCAGTATGATCCATGAGCGCCGTGAATGACGCATGA 1133 0.17230130982452033 No Hit ACCGAGGACTGGGCTGGTATAATGGTTAGGAGTGAGACTCTGGAGTCAGATA 1059 0.1610477379560168 No Hit TCACAGGACTGGGGACATCTGTGATTGCCAGATCAAGGAAATATTTTAAGTT 1041 0.15831038263665106 No Hit ATATAGGACTGGGGCAATGGTTTCTGTTATCAGCAAGTAAATCCTGATTGAT 1022 0.155420952021765 No Hit GTCGAGGACTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC 977 0.1485775637233507 No Hit GGCTAGGACTGGGGATGCTATTTTTTTACTCACAACACAACATAACAAACTG 919 0.13975719658317226 No Hit AGAAAGGACTGGGGGTCTTGTTCCGGGCAAAAGGCCAGCCAAGTCAGGCCCG 888 0.1350428624220424 No Hit TTTCAGGACTGGGAACAATTTCATTTACAATCACATCAAAAAAGAATAAAAT 843 0.1281994741236281 No Hit TCATAGGACTGGGCAGAGTGGTCAGCACTGGGTAAGTAGAAAGACTAAACAA 841 0.12789532353258745 No Hit GTCCAGGACTGGGTCAACGCAGAGTTTACAGGACTGGGTCGCATTGGCTCCT 817 0.12424551644009982 No Hit TTACAGGACTGGGTGCGGGTAACATTTGGGTTACTTGAATACACGTTAGTGA 807 0.12272476348489665 No Hit TTTTAGGACTGGGAAGCAGTGGTATCAATGCAAAGTCGTCAGGACTGGGGGC 792 0.12044363405209187 No Hit ACACAGGACTGGGGAATGGGGAAACCCGGCTGATGAAGAATCAGTCATCCAC 791 0.12029155875657156 No Hit GCTCAGGACTGGGGTTCCCGGGCTGGTCTCAAACTTTCATGCTCAAGTAATC 773 0.11755420343720582 No Hit ACCTAGGACTGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC 761 0.115729299890962 No Hit TGCAAGGACTGGGGTGAAGTTACCCTGGGCCGCAGCAACTCCCTGATGAATG 746 0.11344817045815725 No Hit ACTTAGGACTGGGACCTTCTCCTGAATGACTACTGGGTAAATAAAGAAATGA 746 0.11344817045815725 No Hit GTGAAGGACTGGGTCAACGCAGAGTGTGTAGGACTGGGCCAAATCAGTAAAT 719 0.10934213747910866 No Hit GATCAGGACTGGGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGT 700 0.10645270686422262 No Hit AACAAGGACTGGGGACGGGCCGGGCATGGTGGCTCATGCCTGTAATCCCAGC 693 0.10538817979558039 No Hit TGAAAGGACTGGAGGGAGTTGGAGGAGATTGGATTGGGCTTCCCAGGTGGTG 692 0.10523610450006006 No Hit CATCAGGACTGGGGCTACTCCTCAGTTAGCCACATAGCACTTGTTATTGCAT 685 0.10417157743141785 No Hit GGCAAGGACTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA 682 0.10371535154485689 No Hit GCTGAGGACTGGGGGGAATTCGAGCTCTTGATGATGATACTTAACCAAACCT 677 0.1029549750672553 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.035585619151754413 0.0 0.0 0.0 0.0 12 0.08364141253617491 0.0 0.0 0.0 0.0 13 0.11588137518648232 0.0 0.0 0.0 0.0 14 0.5777340476816881 0.0 0.0 0.0 0.0 15 0.9696320842375477 0.0 0.0 0.0 0.0 16 1.1877080580136838 0.0 0.0 0.0 0.0 17 1.361378045497887 0.0 0.0 0.0 0.0 18 1.609108701900485 0.0 0.0 0.0 0.0 19 1.8239910944706943 0.0 0.0 0.0 0.0 20 2.0449564988617164 0.0 0.0 0.0 0.0 21 2.297249414129924 0.0 0.0 0.0 0.0 22 2.5452842211235627 0.0 0.0 0.0 0.0 23 2.761231140762414 0.0 0.0 0.0 0.0 24 2.99345011702194 0.0 0.0 0.0 0.0 25 3.2477200111319116 0.0 0.0 0.0 0.0 26 3.5441147621010116 0.0 0.0 0.0 0.0 27 3.847504976664046 0.0 0.0 0.0 0.0 28 4.164277817232868 0.0 0.0 0.0 0.0 29 4.5347332371203635 0.0 0.0 0.0 0.0 30 4.978793100039692 0.0 0.0 0.0 0.0 31 5.3731243413238765 0.0 0.0 0.0 0.0 32 5.72852430695486 0.0 0.0 0.0 0.0 33 5.977319490426099 0.0 0.0 0.0 0.0 34 6.2156214785064385 0.0 0.0 0.0 0.0 35 6.4233563321871925 0.0 0.0 0.0 0.0 36 6.642648908327491 0.0 0.0 0.0 0.0 37 6.831830575954767 0.0 0.0 0.0 0.0 38 7.045496366160814 0.0 0.0 0.0 0.0 39 7.256272725751974 0.0 0.0 0.0 0.0 40 7.4627909770685665 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAACTG 110 0.0 46.000004 46 TCGCAGT 105 0.0 46.000004 45 ATTAGCC 55 1.8189894E-12 46.000004 46 TTTACTC 105 0.0 46.000004 25 TTAGCTG 55 1.8189894E-12 46.000004 28 ACGTTAT 55 1.8189894E-12 46.000004 16 TCACAAC 105 0.0 46.000004 30 ATCAGTC 55 1.8189894E-12 46.000004 40 GCTATTT 105 0.0 46.000004 17 TGGGTCC 105 0.0 46.000004 10 TAGAAAT 55 1.8189894E-12 46.000004 41 TTGTCCC 110 0.0 46.000004 34 TTACTCA 105 0.0 46.000004 26 TTGTAGT 55 1.8189894E-12 46.000004 22 CAACATA 105 0.0 46.000004 38 CATTAGC 55 1.8189894E-12 46.000004 45 CGCTCCA 55 1.8189894E-12 46.000004 39 ATTCATT 55 1.8189894E-12 46.000004 23 TTCTTGT 110 0.0 46.000004 31 CTCATTA 55 1.8189894E-12 46.000004 43 >>END_MODULE