SRR1528108_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528108_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619866
Sequences flagged as poor quality	0
Sequence length	59
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGATAGGACTGGGCATTACTTAATATAAATCTAGCTTACAGGGTAAGCCAGATTTTATC	2869	0.4628419690707346	No Hit
TTAGAGGACTGGGATGATAGACCTGCCTTAACAGAGGGACGCTCAGTGGCGCGGATAAA	2193	0.3537861408756086	No Hit
AGTAAGGACTGGGGCGAAACTACGTCTCAAAAAAAAATTATAGGCAAATATTCATAACC	1795	0.28957871539978	No Hit
GAGGAGGACTGGGTGTTGTTGTTGTTGGAGACAGAGCCTCACTCTGCCACCCAGGCTGG	1704	0.27489812314274376	No Hit
GAGGAGGACTGGGGCCGGGAAAAAAACTTTACCTGATGCGTAAGCAACAAAACCATAAA	1580	0.25489379962766145	No Hit
GTCAAGGACTGGGCCCAGTATATTTTTTGAATGCTCAATATAATTAATCATCACAGAAA	1572	0.25360319811055937	No Hit
AATTAGGACTGGGATCGTCCTCAGATCAGGCGAGATCACCCGCTGAATTTAAGCATATT	1506	0.2429557355944672	No Hit
CTAGAGGACTGGGAGCCCTCTGTATTCATGGTTTCTGCATCTGTGGATTCAATCAACCT	1499	0.24182645926700289	No Hit
GTGTAGGACTGAGCGCTCGGCATCACCCAATTTCCCAAACATCTCACGAGATCCAGTTT	1477	0.23827730509497214	No Hit
TCCTAGGACTGGGTACCTATATTTGTATGCATCCTTTTTTTTCATTTCTGTCTGCTAAA	1471	0.23730935395714559	No Hit
GCGCAGGACTGGGAAGCTCCAATACTTGGCCACCAGATGAGAAGAGCTGATTCATTAGA	1425	0.22988839523380858	No Hit
ATCAAGGACTGGGGTTCGCCGTTGTTGGCGGTGCAGTTTGTGCGGGCATATCACCCACT	1338	0.21585310373532343	No Hit
ATACAGGACTGGGCCCCCATTAATAAACCCTGTGTGCCAAACTTCACCCTGATCCTCTC	1320	0.21294925032184375	No Hit
TGTAAGGACTGGGTGGCTCACTGCAACCTCTACCTCCCGGGTTCAAGTGATTCTCCTGC	1230	0.19842998325444533	No Hit
TGATAGGACTGGGGCTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTC	1229	0.19826865806480756	No Hit
GTTAAGGACTGGGTTAAGAGGTAGAGGGTTGGGCGCAGTGGCTCATGTCTGTAATCCCA	1217	0.1963327557891544	No Hit
GGCTAGGACTGGGGGCACAATCCTGAAGTCAGGAGTTCGAGACCAGCCTGACTAACATG	1179	0.19020239858291954	No Hit
ATTAAGGACTGGGGGGGCCGAGGTGGGTGGATTACCTGAGGTCAGGAGTTCAAGACCAG	1163	0.18762119554871537	No Hit
TCATAGGACTGGGGGGAATGAGCCACCTCCTGTTGAAGGCCAGCCCCTCTGTCTCCACC	1118	0.18036156201501613	No Hit
CTTTAGGACTGGGGTCCAGGACTGGGTACTGAAAAAAGCTGACCATTCCAGGAAAGGTG	1100	0.17745770860153645	No Hit
TAGTAGGACTGGGGAAGATAATGGTGACTTCCTTCAAAAGGTCCCCCTACAGGCCACCA	1042	0.16810084760254634	No Hit
GCATAGGACTGGGGTGTTATAAACTTGGATTCCTTCGCAAACAATGACAAAGCAGTTAG	1034	0.16681024608544429	No Hit
TGGAAGGACTGGGGCTTTCAACAATTACAATTCTTGTCCCTTTAGCCCAGAAAAGCCGA	991	0.15987326293102058	No Hit
TCACAGGACTGGGGACATCTGTGATTGCCAGATCAAGGAAATATTTTAAGTTTCTTAAA	948	0.15293627977659688	No Hit
ATATAGGACTGGGGCAATGGTTTCTGTTATCAGCAAGTAAATCCTGATTGATAAAGATA	947	0.1527749545869591	No Hit
ATCTAGGACTGGGCGCTCAGTATGATCCATGAGCGCCGTGAATGACGCATGAGCCTCGT	926	0.14938712560456616	No Hit
ACCGAGGACTGGGCTGGTATAATGGTTAGGAGTGAGACTCTGGAGTCAGATATACCCAA	912	0.14712857294963752	No Hit
ACACAGGACTGGGGAATGGGGAAACCCGGCTGATGAAGAATCAGTCATCCACAGGTAAA	823	0.13277063107187684	No Hit
GGCTAGGACTGGGGATGCTATTTTTTTACTCACAACACAACATAACAAACTGATTAATA	816	0.1316413547444125	No Hit
GTCGAGGACTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA	783	0.1263176234863664	No Hit
TCATAGGACTGGGCAGAGTGGTCAGCACTGGGTAAGTAGAAAGACTAAACAATAAACCA	781	0.1259949731070909	No Hit
AGAAAGGACTGGGGGTCTTGTTCCGGGCAAAAGGCCAGCCAAGTCAGGCCCGAGGCGGT	767	0.12373642045216224	No Hit
TTTCAGGACTGGGAACAATTTCATTTACAATCACATCAAAAAAGAATAAAATGCCTAGG	759	0.12244581893506015	No Hit
TTTTAGGACTGGGAAGCAGTGGTATCAATGCAAAGTCGTCAGGACTGGGGGCAGGCGCC	661	0.10663595035055964	No Hit
GTCCAGGACTGGGTCAACGCAGAGTTTACAGGACTGGGTCGCATTGGCTCCTGAGGCTT	656	0.10582932440237085	No Hit
GCTCAGGACTGGGGTTCCCGGGCTGGTCTCAAACTTTCATGCTCAAGTAATCCTCTTGT	634	0.1022801702303401	No Hit
TTACAGGACTGGGTGCGGGTAACATTTGGGTTACTTGAATACACGTTAGTGAGTGTGGG	627	0.1011508939028758	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATT	15	0.0066948165	53.000004	40
TTGATGC	25	1.477934E-5	53.000004	41
TTTAGCG	30	7.005183E-7	53.000004	45
GTCATAG	15	0.0066948165	53.000004	26
TGTTTAC	15	0.0066948165	53.000004	18
ATGGGCC	15	0.0066948165	53.000004	22
CTATGAG	15	0.0066948165	53.000004	17
CGTGGAG	60	0.0	53.000004	22
CTATGAA	15	0.0066948165	53.000004	23
AGACTAA	60	0.0	53.000004	42
GATACTA	15	0.0066948165	53.000004	13
CCTTAGT	15	0.0066948165	53.000004	37
CCTTACC	15	0.0066948165	53.000004	50
TCGTTCC	15	0.0066948165	53.000004	21
GGAGCTA	15	0.0066948165	53.000004	47
ACTGCAC	50	3.6379788E-12	53.000004	49
TCACCTC	15	0.0066948165	53.000004	33
CAAGTTC	15	0.0066948165	53.000004	49
CCCTCAA	15	0.0066948165	53.000004	52
CTCCGTA	15	0.0066948165	53.000004	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content