##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528108_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619866
Sequences flagged as poor quality	0
Sequence length	59
%GC	40
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.193574095046348	31.0	30.0	31.0	27.0	34.0
2	31.114021417532175	31.0	31.0	34.0	28.0	34.0
3	31.428389361571696	31.0	31.0	34.0	30.0	34.0
4	35.231554884442765	35.0	35.0	37.0	33.0	37.0
5	34.88528649740428	35.0	35.0	37.0	32.0	37.0
6	34.97645781507616	35.0	35.0	37.0	32.0	37.0
7	34.50769682479762	36.0	35.0	37.0	30.0	37.0
8	34.08400847925197	35.0	35.0	37.0	30.0	37.0
9	36.58930155872398	38.0	35.0	39.0	32.0	39.0
10	36.99311464090626	39.0	37.0	39.0	33.0	39.0
11	37.14828688781124	39.0	37.0	39.0	34.0	39.0
12	37.25970129027887	39.0	37.0	39.0	35.0	39.0
13	37.17035133399799	39.0	37.0	39.0	34.0	39.0
14	36.89730845053608	40.0	36.0	40.0	31.0	40.0
15	35.94789035049511	38.0	34.0	40.0	29.0	40.0
16	35.23096766075248	37.0	33.0	40.0	27.0	40.0
17	35.077410278995785	38.0	33.0	40.0	26.0	40.0
18	35.23517502169824	38.0	33.0	40.0	26.0	40.0
19	35.63926719645859	38.0	34.0	40.0	27.0	40.0
20	35.63919137361946	38.0	34.0	40.0	27.0	40.0
21	35.71827620808368	38.0	34.0	40.0	29.0	40.0
22	35.68030832470244	38.0	34.0	40.0	28.0	40.0
23	35.55981615381389	38.0	34.0	40.0	27.0	40.0
24	35.64155478764765	38.0	34.0	40.0	27.0	40.0
25	35.70438288275208	38.0	34.0	40.0	27.0	40.0
26	35.32892592915243	38.0	34.0	40.0	26.0	40.0
27	35.04175579883394	38.0	33.0	40.0	25.0	40.0
28	34.81545204931388	37.0	33.0	40.0	25.0	40.0
29	34.58998880403184	37.0	33.0	40.0	25.0	40.0
30	34.33035043057693	36.0	33.0	40.0	25.0	40.0
31	34.26618333639851	36.0	33.0	40.0	24.0	40.0
32	34.22317242758919	36.0	33.0	40.0	24.0	40.0
33	33.87295479990837	35.0	33.0	40.0	23.0	40.0
34	33.61103528827198	35.0	33.0	40.0	22.0	40.0
35	33.42795378355967	35.0	32.0	39.0	21.0	40.0
36	32.95687616355793	35.0	31.0	39.0	20.0	40.0
37	32.82363123642852	35.0	31.0	39.0	20.0	40.0
38	32.608712205541195	35.0	31.0	39.0	18.0	40.0
39	32.02137397437511	35.0	31.0	38.0	15.0	40.0
40	31.52788344577699	35.0	30.0	38.0	15.0	40.0
41	30.957310128318056	35.0	28.0	38.0	12.0	40.0
42	30.4006866000071	35.0	26.0	38.0	10.0	40.0
43	29.75887046555223	34.0	24.0	38.0	9.0	40.0
44	29.101815553684183	34.0	22.0	37.0	8.0	40.0
45	28.73010618423982	34.0	22.0	37.0	8.0	40.0
46	28.176607524852145	33.0	20.0	37.0	8.0	40.0
47	27.525426463138807	33.0	18.0	36.0	8.0	40.0
48	27.000261346807214	33.0	15.0	36.0	8.0	40.0
49	26.533073277127638	33.0	13.0	35.0	8.0	40.0
50	25.868687748642447	32.0	10.0	35.0	8.0	39.0
51	25.455309695966548	31.0	10.0	35.0	8.0	39.0
52	24.70354076526217	30.0	10.0	35.0	8.0	39.0
53	24.408944836464656	29.0	10.0	35.0	8.0	39.0
54	24.017539274617416	28.0	9.0	35.0	8.0	38.0
55	23.599705420203723	27.0	9.0	35.0	8.0	38.0
56	22.961144828075746	26.0	9.0	35.0	8.0	38.0
57	22.401014090142063	24.0	9.0	35.0	8.0	37.0
58	21.93646530056496	23.0	8.0	34.0	8.0	37.0
59	21.163309489470304	22.0	8.0	33.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	5.0
14	18.0
15	86.0
16	343.0
17	864.0
18	1855.0
19	3357.0
20	5425.0
21	7801.0
22	10861.0
23	14296.0
24	19146.0
25	23583.0
26	28858.0
27	34282.0
28	35911.0
29	32755.0
30	31146.0
31	33444.0
32	37637.0
33	41645.0
34	47057.0
35	49595.0
36	52733.0
37	56434.0
38	46692.0
39	4034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.0469843482301	25.00717897093888	34.95658739146848	3.9892492893625398
2	25.12720491202937	22.042183310586484	34.274665814869664	18.55594596251448
3	18.503192625502933	24.19829447009515	35.39990901259304	21.898603891808875
4	19.58729789986868	25.578754117825465	33.19830414960653	21.635643832699326
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.28420013357726	0.5243068663227214	0.11470220983244765	0.07679079026757396
12	98.49031887536984	1.202033987990953	0.15826001103464296	0.14938712560456616
13	95.71439633727289	2.848357548244298	0.8798675842843453	0.5573785301984623
14	48.82700454614384	29.501698754246885	11.530879254548564	10.140417445060706
15	33.951692785214874	36.01988171637095	21.178448245265912	8.84997725314826
16	39.069573101283176	27.48803773718836	19.983835215998297	13.458553945530163
17	22.742173308424725	31.844947133735356	19.475499543449715	25.937380014390204
18	20.159518347513817	42.892657445318825	21.24265567074174	15.705168536425615
19	24.77809720165326	42.469985448467895	22.045409814379237	10.706507535499608
20	19.305462793571515	39.99735426688994	27.059074057941558	13.638108881596992
21	24.789067314548628	40.95385131625222	15.706781788321992	18.55029958087716
22	24.501101851045227	45.683260575672804	15.506415902791893	14.309221670490075
23	18.4218847299255	44.96858998557753	21.91489773596229	14.694627548534683
24	18.754375945768924	48.30608550880351	15.931346452297754	17.008192093129807
25	23.498143147067267	43.369857356267325	18.434629419906884	14.697370076758526
26	18.380585481378233	45.433367856923915	15.706781788321992	20.479264873375858
27	16.302233063274965	52.899658958549104	15.06712741140827	15.730980566767656
28	16.473237764290992	52.41519941406692	12.290559572552777	18.82100324908932
29	18.869562131170284	50.65949737523916	13.220921941193742	17.25001855239681
30	23.723998412560135	52.73026750942946	12.306530766326915	11.239203311683493
31	16.91284890605389	56.14842562747433	11.76947921002281	15.169246256448973
32	17.215656287003966	59.42881203356855	13.175589562905532	10.179942116521957
33	18.67984370815628	57.93574740347107	12.829385705942897	10.555023182429752
34	17.555084486001814	58.75463406607234	12.011305669289813	11.678975778636028
35	20.194848564044488	57.051040063497595	12.585139368831328	10.16897200362659
36	17.457805396650244	55.834970784008156	16.265450920037555	10.441772899304043
37	16.843963050078564	56.92891689494181	14.242594367169678	11.984525687809947
38	16.644081140117382	56.26554771515134	14.939196536025529	12.151174608705753
39	15.451081362746141	56.5157630842795	15.264266793145614	12.768888759828737
40	17.55814966460493	56.23586388025799	12.60223983893293	13.603746616204148
41	19.719423230182006	56.167945975420494	13.709414615416883	10.40321617898062
42	22.352411650259896	52.097227465290885	13.571965553845509	11.97839533060371
43	22.953186656470915	50.81001377717119	13.462425750081469	12.774373816276421
44	18.39381414692853	52.46053179235513	14.302446012525289	14.843208048191062
45	18.50916165751953	48.920411830944104	15.588530424317515	16.98189608721885
46	21.293795755856912	49.02091742408843	16.734584571504165	12.950702248550494
47	23.569126230507884	49.5116686509665	13.533570158711722	13.385634959813894
48	23.537506493338885	48.396750265379936	13.36079088060968	14.7049523606715
49	22.430170391665296	50.383953951337865	12.70016422904305	14.485711427953785
50	19.903172621179415	52.690581512778564	12.84148509516573	14.564760770876287
51	20.619456463171073	51.6495500640461	15.232647055976614	12.498346416806214
52	21.273468781962553	48.96558288404268	14.649133845056836	15.111814488937933
53	19.168981683137968	48.85862428331285	14.738346674926516	17.23404735862267
54	19.663443389377704	50.01952034794617	14.43037688790803	15.886659374768094
55	21.198291243591356	47.35233098766508	14.697208751568889	16.75216901717468
56	19.84945133303004	44.54882184214007	18.775670870801108	16.826055954028774
57	21.63612780826824	45.34157382402003	15.726624786647436	17.295673581064293
58	22.93769943826569	41.88937609096159	17.01174124730184	18.16118322347088
59	22.15898274788422	41.332642861521684	18.541104045067804	17.96727034552629
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	2.0
5	4.0
6	4.0
7	4.0
8	78.0
9	316.0
10	480.0
11	695.0
12	910.0
13	1633.0
14	3350.0
15	4344.0
16	4099.0
17	3854.0
18	3646.0
19	3959.0
20	4480.0
21	5199.5
22	5919.0
23	8125.5
24	10332.0
25	13309.5
26	19746.0
27	23205.0
28	25816.5
29	28428.0
30	30586.0
31	33209.0
32	33674.0
33	34019.0
34	34364.0
35	36829.5
36	34242.5
37	29190.0
38	26774.5
39	24359.0
40	24500.0
41	24641.0
42	24806.0
43	25689.5
44	26408.0
45	28548.0
46	30688.0
47	29701.5
48	28494.5
49	28274.0
50	32205.5
51	36137.0
52	33870.0
53	28107.0
54	24611.0
55	23387.5
56	22164.0
57	19072.0
58	13004.0
59	10028.0
60	9273.5
61	8519.0
62	6828.5
63	5138.0
64	3633.5
65	1419.0
66	709.0
67	516.5
68	324.0
69	533.5
70	440.5
71	138.0
72	99.5
73	61.0
74	47.0
75	24.5
76	16.0
77	11.0
78	6.0
79	5.5
80	5.0
81	4.0
82	3.0
83	3.0
84	1.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	619866.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.83473739693959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.17908271820056	57.617041107676435
2	3.370124490900374	4.167815257796387
3	0.9981416490614742	1.8515948026399345
4	0.4581948803580934	1.1332944041425943
5	0.27538269932581244	0.8514108448235993
6	0.198566616430416	0.7366988749664163
7	0.15463848379110848	0.6693421025678865
8	0.11283741476044058	0.5581817528209119
9	0.09740782388530435	0.5420868489319621
>10	0.9967794604802725	13.587373822327297
>50	0.09746632955988066	3.9881158190373176
>100	0.04794288777181032	5.821566603710855
>500	0.007639251348255008	3.253170906807759
>1k	0.005795294126262419	5.222306851750629
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGATAGGACTGGGCATTACTTAATATAAATCTAGCTTACAGGGTAAGCCAGATTTTATC	2869	0.4628419690707346	No Hit
TTAGAGGACTGGGATGATAGACCTGCCTTAACAGAGGGACGCTCAGTGGCGCGGATAAA	2193	0.3537861408756086	No Hit
AGTAAGGACTGGGGCGAAACTACGTCTCAAAAAAAAATTATAGGCAAATATTCATAACC	1795	0.28957871539978	No Hit
GAGGAGGACTGGGTGTTGTTGTTGTTGGAGACAGAGCCTCACTCTGCCACCCAGGCTGG	1704	0.27489812314274376	No Hit
GAGGAGGACTGGGGCCGGGAAAAAAACTTTACCTGATGCGTAAGCAACAAAACCATAAA	1580	0.25489379962766145	No Hit
GTCAAGGACTGGGCCCAGTATATTTTTTGAATGCTCAATATAATTAATCATCACAGAAA	1572	0.25360319811055937	No Hit
AATTAGGACTGGGATCGTCCTCAGATCAGGCGAGATCACCCGCTGAATTTAAGCATATT	1506	0.2429557355944672	No Hit
CTAGAGGACTGGGAGCCCTCTGTATTCATGGTTTCTGCATCTGTGGATTCAATCAACCT	1499	0.24182645926700289	No Hit
GTGTAGGACTGAGCGCTCGGCATCACCCAATTTCCCAAACATCTCACGAGATCCAGTTT	1477	0.23827730509497214	No Hit
TCCTAGGACTGGGTACCTATATTTGTATGCATCCTTTTTTTTCATTTCTGTCTGCTAAA	1471	0.23730935395714559	No Hit
GCGCAGGACTGGGAAGCTCCAATACTTGGCCACCAGATGAGAAGAGCTGATTCATTAGA	1425	0.22988839523380858	No Hit
ATCAAGGACTGGGGTTCGCCGTTGTTGGCGGTGCAGTTTGTGCGGGCATATCACCCACT	1338	0.21585310373532343	No Hit
ATACAGGACTGGGCCCCCATTAATAAACCCTGTGTGCCAAACTTCACCCTGATCCTCTC	1320	0.21294925032184375	No Hit
TGTAAGGACTGGGTGGCTCACTGCAACCTCTACCTCCCGGGTTCAAGTGATTCTCCTGC	1230	0.19842998325444533	No Hit
TGATAGGACTGGGGCTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTC	1229	0.19826865806480756	No Hit
GTTAAGGACTGGGTTAAGAGGTAGAGGGTTGGGCGCAGTGGCTCATGTCTGTAATCCCA	1217	0.1963327557891544	No Hit
GGCTAGGACTGGGGGCACAATCCTGAAGTCAGGAGTTCGAGACCAGCCTGACTAACATG	1179	0.19020239858291954	No Hit
ATTAAGGACTGGGGGGGCCGAGGTGGGTGGATTACCTGAGGTCAGGAGTTCAAGACCAG	1163	0.18762119554871537	No Hit
TCATAGGACTGGGGGGAATGAGCCACCTCCTGTTGAAGGCCAGCCCCTCTGTCTCCACC	1118	0.18036156201501613	No Hit
CTTTAGGACTGGGGTCCAGGACTGGGTACTGAAAAAAGCTGACCATTCCAGGAAAGGTG	1100	0.17745770860153645	No Hit
TAGTAGGACTGGGGAAGATAATGGTGACTTCCTTCAAAAGGTCCCCCTACAGGCCACCA	1042	0.16810084760254634	No Hit
GCATAGGACTGGGGTGTTATAAACTTGGATTCCTTCGCAAACAATGACAAAGCAGTTAG	1034	0.16681024608544429	No Hit
TGGAAGGACTGGGGCTTTCAACAATTACAATTCTTGTCCCTTTAGCCCAGAAAAGCCGA	991	0.15987326293102058	No Hit
TCACAGGACTGGGGACATCTGTGATTGCCAGATCAAGGAAATATTTTAAGTTTCTTAAA	948	0.15293627977659688	No Hit
ATATAGGACTGGGGCAATGGTTTCTGTTATCAGCAAGTAAATCCTGATTGATAAAGATA	947	0.1527749545869591	No Hit
ATCTAGGACTGGGCGCTCAGTATGATCCATGAGCGCCGTGAATGACGCATGAGCCTCGT	926	0.14938712560456616	No Hit
ACCGAGGACTGGGCTGGTATAATGGTTAGGAGTGAGACTCTGGAGTCAGATATACCCAA	912	0.14712857294963752	No Hit
ACACAGGACTGGGGAATGGGGAAACCCGGCTGATGAAGAATCAGTCATCCACAGGTAAA	823	0.13277063107187684	No Hit
GGCTAGGACTGGGGATGCTATTTTTTTACTCACAACACAACATAACAAACTGATTAATA	816	0.1316413547444125	No Hit
GTCGAGGACTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA	783	0.1263176234863664	No Hit
TCATAGGACTGGGCAGAGTGGTCAGCACTGGGTAAGTAGAAAGACTAAACAATAAACCA	781	0.1259949731070909	No Hit
AGAAAGGACTGGGGGTCTTGTTCCGGGCAAAAGGCCAGCCAAGTCAGGCCCGAGGCGGT	767	0.12373642045216224	No Hit
TTTCAGGACTGGGAACAATTTCATTTACAATCACATCAAAAAAGAATAAAATGCCTAGG	759	0.12244581893506015	No Hit
TTTTAGGACTGGGAAGCAGTGGTATCAATGCAAAGTCGTCAGGACTGGGGGCAGGCGCC	661	0.10663595035055964	No Hit
GTCCAGGACTGGGTCAACGCAGAGTTTACAGGACTGGGTCGCATTGGCTCCTGAGGCTT	656	0.10582932440237085	No Hit
GCTCAGGACTGGGGTTCCCGGGCTGGTCTCAAACTTTCATGCTCAAGTAATCCTCTTGT	634	0.1022801702303401	No Hit
TTACAGGACTGGGTGCGGGTAACATTTGGGTTACTTGAATACACGTTAGTGAGTGTGGG	627	0.1011508939028758	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.03452359058248073	0.0	0.0	0.0	0.0
12	0.0750162131815586	0.0	0.0	0.0	0.0
13	0.10534534883345756	0.0	0.0	0.0	0.0
14	0.503011941290537	0.0	0.0	0.0	0.0
15	0.8351805067546857	0.0	0.0	0.0	0.0
16	1.025544230527243	0.0	0.0	0.0	0.0
17	1.1715435271494161	0.0	0.0	0.0	0.0
18	1.382395550005969	0.0	0.0	0.0	0.0
19	1.567596867710118	0.0	0.0	0.0	0.0
20	1.7616710708443437	0.0	0.0	0.0	0.0
21	1.9697805654770548	0.0	0.0	0.0	0.0
22	2.1633707930423673	0.0	0.0	0.0	0.0
23	2.3369566970925972	0.0	0.0	0.0	0.0
24	2.532482826933563	0.0	0.0	0.0	0.0
25	2.7362365414460545	0.0	0.0	0.0	0.0
26	2.9916143166426292	0.0	0.0	0.0	0.0
27	3.2431202872878977	0.0	0.0	0.0	0.0
28	3.492851680847151	0.0	0.0	0.0	0.0
29	3.8142114586055698	0.0	0.0	0.0	0.0
30	4.189776500082276	0.0	0.0	0.0	0.0
31	4.515653383150552	0.0	0.0	0.0	0.0
32	4.816363536635337	0.0	0.0	0.0	0.0
33	5.02866748619863	0.0	0.0	0.0	0.0
34	5.234034452607499	0.0	0.0	0.0	0.0
35	5.410362884881571	0.0	0.0	0.0	0.0
36	5.590240471327674	0.0	0.0	0.0	0.0
37	5.763987700567542	0.0	0.0	0.0	0.0
38	5.931120597032262	0.0	0.0	0.0	0.0
39	6.1042225255135785	0.0	0.0	0.0	0.0
40	6.284261437149319	0.0	0.0	0.0	0.0
41	6.470430705991295	0.0	0.0	0.0	0.0
42	6.642564683334785	0.0	0.0	0.0	0.0
43	6.798727466904137	0.0	0.0	0.0	0.0
44	6.952954348197836	0.0	0.0	0.0	0.0
45	7.101050872285301	0.0	0.0	0.0	0.0
46	7.245275591821458	0.0	0.0	0.0	0.0
47	7.370786589359636	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATT	15	0.0066948165	53.000004	40
TTGATGC	25	1.477934E-5	53.000004	41
TTTAGCG	30	7.005183E-7	53.000004	45
GTCATAG	15	0.0066948165	53.000004	26
TGTTTAC	15	0.0066948165	53.000004	18
ATGGGCC	15	0.0066948165	53.000004	22
CTATGAG	15	0.0066948165	53.000004	17
CGTGGAG	60	0.0	53.000004	22
CTATGAA	15	0.0066948165	53.000004	23
AGACTAA	60	0.0	53.000004	42
GATACTA	15	0.0066948165	53.000004	13
CCTTAGT	15	0.0066948165	53.000004	37
CCTTACC	15	0.0066948165	53.000004	50
TCGTTCC	15	0.0066948165	53.000004	21
GGAGCTA	15	0.0066948165	53.000004	47
ACTGCAC	50	3.6379788E-12	53.000004	49
TCACCTC	15	0.0066948165	53.000004	33
CAAGTTC	15	0.0066948165	53.000004	49
CCCTCAA	15	0.0066948165	53.000004	52
CTCCGTA	15	0.0066948165	53.000004	17
>>END_MODULE