FastQCFastQC Report
Fri 17 Jun 2016
SRR1528107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5359683
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAAAGTTGGGACCTGCTGGGATTTATTCAAGCCAAACTTTAGTGATGAG65770.12271248131652562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA405.6188583E-946.00000428
CATATTA206.3135335E-446.00000436
CGACCTA800.046.00000445
CCTAATT206.3135335E-446.00000427
CGCGACA405.6188583E-946.00000427
TAGTTAG206.3135335E-446.00000439
TAGTTAC405.6188583E-946.00000418
TAGCACA1600.046.00000419
CCCAATA405.6188583E-946.00000446
CTAGTTT206.3135335E-446.00000432
TTAAGTG206.3135335E-446.00000439
TATTCTA206.3135335E-446.00000428
TTGACCG405.6188583E-946.00000436
TCTAAAC405.6188583E-946.00000420
ACTAATA405.6188583E-946.00000431
TTAACTA405.6188583E-946.00000428
TATAGGC405.6188583E-946.00000416
CTCTATA405.6188583E-946.00000417
ATATTCG405.6188583E-946.00000415
GATAGGT206.3135335E-446.00000413