##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5359683
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.80647624122546	31.0	31.0	33.0	30.0	33.0
2	31.620842501319576	31.0	31.0	34.0	30.0	34.0
3	31.965781931506022	31.0	31.0	34.0	30.0	34.0
4	35.71893095169994	37.0	35.0	37.0	35.0	37.0
5	35.48482139708636	37.0	35.0	37.0	33.0	37.0
6	35.82753494936174	37.0	35.0	37.0	35.0	37.0
7	35.633991973032735	37.0	35.0	37.0	33.0	37.0
8	35.70415563756289	37.0	35.0	37.0	33.0	37.0
9	35.96138540283073	37.0	35.0	39.0	32.0	39.0
10	37.15845470711607	39.0	37.0	39.0	34.0	39.0
11	37.13034464911451	39.0	37.0	39.0	34.0	39.0
12	37.39755634801536	39.0	37.0	39.0	35.0	39.0
13	37.31969316095747	39.0	37.0	39.0	35.0	39.0
14	37.90844906312556	40.0	38.0	40.0	33.0	40.0
15	37.19024744560453	39.0	37.0	40.0	32.0	40.0
16	37.06345655144157	39.0	36.0	40.0	32.0	40.0
17	36.96349168411639	39.0	36.0	40.0	32.0	40.0
18	37.06827530658063	39.0	36.0	40.0	32.0	40.0
19	37.20502238658518	39.0	36.0	40.0	32.0	40.0
20	37.25067228789464	39.0	36.0	40.0	32.0	40.0
21	37.18992242638231	39.0	36.0	40.0	32.0	40.0
22	37.27320496380103	39.0	36.0	40.0	32.0	40.0
23	37.20135985654375	39.0	36.0	40.0	32.0	40.0
24	37.17924810105374	39.0	36.0	40.0	32.0	40.0
25	37.293131142270916	40.0	36.0	40.0	32.0	40.0
26	37.12971364164634	40.0	36.0	40.0	32.0	40.0
27	36.93461721523456	39.0	36.0	40.0	31.0	40.0
28	36.80826459326046	39.0	36.0	40.0	31.0	40.0
29	36.83256640364738	39.0	35.0	40.0	31.0	40.0
30	36.74690163578704	39.0	35.0	40.0	31.0	40.0
31	36.736816524410116	39.0	35.0	40.0	31.0	40.0
32	36.69628483624871	39.0	35.0	40.0	31.0	40.0
33	36.5836285466883	39.0	35.0	40.0	31.0	40.0
34	36.4490657376565	39.0	35.0	40.0	31.0	40.0
35	36.37088182267496	39.0	35.0	40.0	30.0	40.0
36	36.23152283446614	38.0	35.0	40.0	30.0	40.0
37	36.097862317603486	38.0	35.0	40.0	30.0	40.0
38	35.86023930892928	38.0	35.0	40.0	30.0	40.0
39	35.63579655737102	38.0	35.0	40.0	29.0	40.0
40	35.44727122854094	38.0	35.0	40.0	28.0	40.0
41	35.16442950077458	38.0	34.0	40.0	27.0	40.0
42	34.87245663596149	37.0	34.0	40.0	26.0	40.0
43	34.54682748961086	37.0	33.0	40.0	25.0	40.0
44	34.238199908464736	36.0	33.0	40.0	24.0	40.0
45	34.022606374294895	36.0	33.0	40.0	23.0	40.0
46	33.80227095520388	36.0	33.0	40.0	23.0	40.0
47	33.420541476053714	35.0	33.0	39.0	21.0	40.0
48	33.17680280718095	35.0	33.0	39.0	20.0	40.0
49	33.00098065501262	35.0	33.0	39.0	20.0	40.0
50	32.7705573258717	35.0	32.0	39.0	19.0	40.0
51	32.48399261672752	35.0	32.0	39.0	18.0	40.0
52	31.601490050810842	35.0	31.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	2.0
14	7.0
15	40.0
16	159.0
17	541.0
18	1246.0
19	2661.0
20	5107.0
21	8605.0
22	13635.0
23	20247.0
24	29337.0
25	41282.0
26	56601.0
27	74870.0
28	98681.0
29	127154.0
30	157896.0
31	184942.0
32	211354.0
33	255955.0
34	339975.0
35	460296.0
36	618540.0
37	986735.0
38	1375102.0
39	288707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.89307427323594	16.613482551113563	33.50711226764717	13.986330908003328
2	32.822724776819825	18.015766977263397	30.709185599222938	18.452322646693844
3	19.28817431926478	23.948020806454412	36.03196308438391	20.7318417898969
4	17.70905107634164	26.042659612518126	38.66473073127646	17.583558579863773
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.43412698101734	0.28484893602849276	0.2583361739864093	0.02268790896775052
12	99.35994348919517	0.46508347601901084	0.07935170792750243	0.09562132685832352
13	98.03736526955046	1.1655540075784332	0.28016582323991923	0.5169148996311909
14	52.65578206770811	32.963330107396274	5.697314561327601	8.683573263568013
15	28.93038636799975	31.218581397444588	19.680007194455342	20.171025040100318
16	30.599888090396394	19.990361370252682	25.972170369031154	23.437580170319773
17	21.837000434540624	20.504477596902653	27.603964637460837	30.05455733109589
18	21.538008871047037	26.665494955578527	29.133364790417644	22.66313138295679
19	24.295989147119336	24.510554075679476	26.498395520779866	24.695061256421322
20	24.320934652291935	23.831614668255565	27.316615553569118	24.530835125883378
21	29.97944094827996	25.39806925148372	18.615951726995796	26.006538073240527
22	29.019813298659642	27.655180353017144	19.635415004954584	23.68959134336863
23	24.111892438414735	27.673744883792565	22.888032743727567	25.32632993406513
24	24.139599300928804	31.220894967109064	19.805163850175468	24.834341881786663
25	27.573682995804045	24.70828965071255	24.126874667774196	23.59115268570921
26	25.741074612061944	26.635940968896854	18.779188993080375	28.843795425960828
27	26.930715865098737	31.45941653638844	17.93753473852838	23.672332859984444
28	25.72079356185804	31.600637575020762	17.685840748417398	24.992728114703798
29	26.481846034550927	29.43295340414722	17.67113838635606	26.414062174945798
30	28.760227050741623	29.267775724795662	18.1902362509126	23.781760973550114
31	26.09103933945347	29.212529920146395	18.32580397012286	26.370626770277273
32	25.700139355256646	33.366973382567586	17.784204774797317	23.14868248737845
33	26.755798803772535	33.181290759173635	18.25410196834402	21.80880846870981
34	25.393236876136143	34.93068526627414	16.594209023182902	23.08186883440681
35	25.604014267261704	34.53165047261191	17.96080103991225	21.903534220214144
36	26.922599713453206	32.60677916958895	18.46428230923359	22.006338807724262
37	25.75523589734691	33.38173171808855	18.866544905734166	21.99648747883037
38	24.73890713312709	34.905590498542544	19.386389083085696	20.969113285244667
39	26.630623490232537	33.56306706945168	19.79572299331882	20.01058644699696
40	26.222035892794405	34.54303174273553	18.141688603598386	21.093243760871676
41	26.43684337301292	35.605128139108224	16.80418412805384	21.153844359825012
42	26.74156288720807	35.75032329337388	16.773305436161056	20.734808383256993
43	27.313294461631404	33.59961773858641	17.38007639630926	21.70701140347293
44	26.596069207824417	32.822538198621075	18.532047511018842	22.049345082535666
45	25.573490073946537	31.91295082190495	20.80802166844569	21.70553743570282
46	25.827329713343122	30.726910528103996	20.68038352268222	22.765376235870665
47	27.411322647253577	29.927180394810666	20.01659426499664	22.644902692939116
48	27.029919493373022	30.73133243141432	19.632821567991986	22.60592650722067
49	26.893903986485768	30.1786878067229	20.187779762347883	22.73962844444345
50	25.870615855452645	32.15451361582392	19.726521139403207	22.248349389320225
51	24.793201388962743	32.02762178285544	20.537837778838785	22.641339049343028
52	26.27480020740033	31.521677681310628	19.769937886251853	22.43358422503719
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	3.5
2	7.0
3	10.5
4	14.0
5	163.5
6	313.0
7	1798.5
8	3284.0
9	5753.5
10	8223.0
11	10917.0
12	13611.0
13	15784.5
14	15912.0
15	13866.0
16	16803.0
17	19740.0
18	25407.0
19	31074.0
20	45664.5
21	60255.0
22	82369.0
23	104483.0
24	128786.0
25	153089.0
26	157789.0
27	162489.0
28	148018.0
29	133547.0
30	120519.0
31	107491.0
32	102449.0
33	97407.0
34	98899.5
35	100392.0
36	99695.5
37	98999.0
38	109136.5
39	140352.5
40	161431.0
41	181728.0
42	202025.0
43	208477.5
44	214930.0
45	228728.5
46	242527.0
47	251279.0
48	260031.0
49	285080.5
50	310130.0
51	318265.5
52	326401.0
53	339838.0
54	353275.0
55	387507.5
56	421740.0
57	403502.5
58	385265.0
59	373035.5
60	360806.0
61	311580.0
62	262354.0
63	234672.5
64	185163.0
65	163335.0
66	136070.5
67	108806.0
68	89264.5
69	69723.0
70	51116.0
71	32509.0
72	25215.0
73	17921.0
74	13582.5
75	9244.0
76	6711.5
77	4179.0
78	2250.5
79	322.0
80	208.5
81	95.0
82	92.5
83	90.0
84	60.5
85	31.0
86	18.5
87	6.0
88	3.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5359683.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.596309525941454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.6816956572621	12.739330080744073
2	7.377569118006119	2.3012570302690065
3	2.655624704377346	1.2425383462261759
4	1.4297704760424574	0.891965715816433
5	0.912757428252752	0.7117823686566109
6	0.6970836615210612	0.6523159530335444
7	0.5177227941748451	0.5652195462610314
8	0.4319467035123333	0.5389419589350729
9	0.3436591069012709	0.48238324223767415
>10	2.9173451096021257	9.466837006054478
>50	0.29482423855928874	3.141892043675015
>100	0.37645220577349175	15.295718850809598
>500	0.2562070785416586	28.415845234223973
>1k	0.10721776629827513	23.42682731592071
>5k	1.2395117491130071E-4	0.12714530713666505
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAACAAAGTTGGGACCTGCTGGGATTTATTCAAGCCAAACTTTAGTGATGAG	6577	0.12271248131652562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.009272936477773032	0.0	0.0	0.0	0.0
12	0.020896758259770214	0.0	0.0	0.0	0.0
13	0.04849167385459177	0.0	0.0	0.0	0.0
14	0.20794140250458842	0.0	0.0	0.0	0.0
15	0.39603088466239517	0.0	0.0	0.0	0.0
16	0.4852898949434136	0.0	0.0	0.0	0.0
17	0.5474204351264804	0.0	0.0	0.0	0.0
18	0.6470905088976344	0.0	0.0	0.0	0.0
19	0.7238674376824151	0.0	0.0	0.0	0.0
20	0.799543555094583	0.0	0.0	0.0	0.0
21	0.8786153957239635	0.0	0.0	0.0	0.0
22	0.9428542695528821	0.0	0.0	0.0	0.0
23	1.0052087035744466	0.0	0.0	0.0	0.0
24	1.0878628456197876	0.0	0.0	0.0	0.0
25	1.1687258369571485	0.0	0.0	0.0	0.0
26	1.2678921495916828	0.0	0.0	0.0	0.0
27	1.3941309588645447	0.0	0.0	0.0	0.0
28	1.505965931194065	0.0	0.0	0.0	0.0
29	1.621289915840172	0.0	0.0	0.0	0.0
30	1.753238017994721	0.0	0.0	0.0	0.0
31	1.8816224765531842	0.0	0.0	0.0	0.0
32	2.04114683648268	0.0	0.0	0.0	0.0
33	2.154735643880431	0.0	0.0	0.0	0.0
34	2.2541071925335885	0.0	0.0	0.0	0.0
35	2.347806763944808	0.0	0.0	0.0	0.0
36	2.4445102443558695	0.0	0.0	0.0	0.0
37	2.5383777361459625	0.0	0.0	0.0	0.0
38	2.63452148196078	0.0	0.0	0.0	0.0
39	2.7327922192413245	0.0	0.0	0.0	0.0
40	2.8393843441860276	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCTA	40	5.6188583E-9	46.000004	28
CATATTA	20	6.3135335E-4	46.000004	36
CGACCTA	80	0.0	46.000004	45
CCTAATT	20	6.3135335E-4	46.000004	27
CGCGACA	40	5.6188583E-9	46.000004	27
TAGTTAG	20	6.3135335E-4	46.000004	39
TAGTTAC	40	5.6188583E-9	46.000004	18
TAGCACA	160	0.0	46.000004	19
CCCAATA	40	5.6188583E-9	46.000004	46
CTAGTTT	20	6.3135335E-4	46.000004	32
TTAAGTG	20	6.3135335E-4	46.000004	39
TATTCTA	20	6.3135335E-4	46.000004	28
TTGACCG	40	5.6188583E-9	46.000004	36
TCTAAAC	40	5.6188583E-9	46.000004	20
ACTAATA	40	5.6188583E-9	46.000004	31
TTAACTA	40	5.6188583E-9	46.000004	28
TATAGGC	40	5.6188583E-9	46.000004	16
CTCTATA	40	5.6188583E-9	46.000004	17
ATATTCG	40	5.6188583E-9	46.000004	15
GATAGGT	20	6.3135335E-4	46.000004	13
>>END_MODULE