FastQCFastQC Report
Fri 17 Jun 2016
SRR1528106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5320759
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAAAGTTGGGACCTGCTGGGATTTATTCAAGCCAAACTTTAGTGATGAG65660.12340344676389214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACG351.0205076E-746.00000446
CCAATAC700.046.00000426
CTGTTAG351.0205076E-746.00000436
TAAGACG351.0205076E-746.00000417
TACACTA351.0205076E-746.00000434
ACGACTA1050.046.00000426
AACGTTC405.6188583E-946.029
TGATAGC800.046.023
CTACTTA206.3135306E-446.032
ATAGGGT750.046.019
TTTACGT253.41884E-546.021
TAGACCC800.046.033
GTTTACG600.046.018
ATTACCC551.8189894E-1246.033
TTCACGG800.046.021
CGCGCAT206.3135306E-446.045
CGATTTA1000.046.034
TACGGTC1600.046.027
TAACGTA501.6370905E-1146.027
CGATTAG551.8189894E-1246.041
Produced by FastQC (version 0.11.5)