##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528106_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5320759
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.831561061119288	31.0	31.0	33.0	30.0	33.0
2	31.640791097661065	31.0	31.0	34.0	30.0	34.0
3	31.978989087835025	31.0	31.0	34.0	30.0	34.0
4	35.745133917924115	37.0	35.0	37.0	35.0	37.0
5	35.608664478131786	37.0	35.0	37.0	33.0	37.0
6	35.86972027862942	37.0	35.0	37.0	35.0	37.0
7	35.62013389443123	37.0	35.0	37.0	33.0	37.0
8	35.6889287787701	37.0	35.0	37.0	33.0	37.0
9	35.98137126676852	37.0	35.0	39.0	32.0	39.0
10	37.14070267042728	39.0	37.0	39.0	34.0	39.0
11	37.109651461379855	39.0	37.0	39.0	34.0	39.0
12	37.411273466811785	39.0	37.0	39.0	35.0	39.0
13	37.31307882954293	39.0	37.0	39.0	35.0	39.0
14	37.85438430870483	40.0	38.0	40.0	33.0	40.0
15	37.18946469855147	39.0	37.0	40.0	32.0	40.0
16	37.038695795092394	39.0	36.0	40.0	32.0	40.0
17	36.956352467758826	39.0	36.0	40.0	32.0	40.0
18	36.98150545814986	39.0	36.0	40.0	32.0	40.0
19	37.18910290806255	39.0	36.0	40.0	32.0	40.0
20	37.24347391039512	39.0	36.0	40.0	32.0	40.0
21	37.2251943754641	39.0	36.0	40.0	32.0	40.0
22	37.24053335247847	39.0	36.0	40.0	32.0	40.0
23	37.26070660219717	39.0	36.0	40.0	32.0	40.0
24	37.29585064837554	39.0	36.0	40.0	32.0	40.0
25	37.322276201571995	40.0	37.0	40.0	32.0	40.0
26	37.0387786780044	40.0	36.0	40.0	31.0	40.0
27	36.813224203539384	39.0	36.0	40.0	31.0	40.0
28	36.72611050415927	39.0	36.0	40.0	31.0	40.0
29	36.75013264085067	39.0	35.0	40.0	31.0	40.0
30	36.552444679415096	39.0	35.0	40.0	30.0	40.0
31	36.588810731702	39.0	35.0	40.0	30.0	40.0
32	36.633536305628574	39.0	35.0	40.0	31.0	40.0
33	36.62748209418994	39.0	35.0	40.0	31.0	40.0
34	36.53817716607725	39.0	35.0	40.0	31.0	40.0
35	36.28541134826817	39.0	35.0	40.0	30.0	40.0
36	36.19808997175027	38.0	35.0	40.0	30.0	40.0
37	36.07237708003689	38.0	35.0	40.0	30.0	40.0
38	35.88889010007783	38.0	35.0	40.0	30.0	40.0
39	35.67301018520102	38.0	35.0	40.0	29.0	40.0
40	35.419908325109255	38.0	35.0	40.0	28.0	40.0
41	35.10887206129802	38.0	34.0	40.0	27.0	40.0
42	34.81530398200708	37.0	34.0	40.0	26.0	40.0
43	34.52505610571725	37.0	33.0	40.0	25.0	40.0
44	34.12736791874994	36.0	33.0	40.0	23.0	40.0
45	33.911364713192235	36.0	33.0	40.0	23.0	40.0
46	33.68873143850342	36.0	33.0	39.0	23.0	40.0
47	33.44448226277492	35.0	33.0	39.0	22.0	40.0
48	33.1915873280485	35.0	33.0	39.0	20.0	40.0
49	32.82868571946221	35.0	32.0	39.0	20.0	40.0
50	32.60264766737227	35.0	32.0	39.0	18.0	40.0
51	32.31632686238937	35.0	32.0	38.0	17.0	40.0
52	31.123893602397704	35.0	30.0	37.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	3.0
14	11.0
15	60.0
16	189.0
17	509.0
18	1253.0
19	2641.0
20	5015.0
21	8402.0
22	13473.0
23	20596.0
24	29731.0
25	41674.0
26	56433.0
27	75588.0
28	99504.0
29	128360.0
30	158703.0
31	185979.0
32	211632.0
33	257578.0
34	341014.0
35	459143.0
36	619518.0
37	988576.0
38	1362310.0
39	252860.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.917225343226406	16.624545483078638	33.50256984012995	13.955659333565004
2	32.87589233039873	17.980912121748045	30.74888375887726	18.39431178897597
3	19.280557529480287	23.95744667255179	36.04401176599053	20.717984031977394
4	17.69407334555089	26.011796437312796	38.69447949061403	17.599650726522288
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.44118874769559	0.27597566437419924	0.26033879752869843	0.022496790401519785
12	99.39608240102586	0.44091453869645286	0.07562830791622023	0.08737475236145821
13	98.06281397071358	1.154797651989124	0.27398346739628693	0.5084049099010123
14	52.65228137564585	32.986910326139565	5.702156402874102	8.658651895340496
15	28.91610012782011	31.360732557140814	19.50832578585123	20.214841529187847
16	30.699999755673957	20.01231403264083	25.90587169988342	23.381814511801792
17	21.817751941029464	20.492377121384376	27.623145494843875	30.066725442742285
18	21.49729014225226	26.765391929985928	29.097953882143507	22.639364045618304
19	24.34985309426719	24.48272887383172	26.458687566942984	24.7087304649581
20	24.285576550262846	23.82652550134295	27.34940635349205	24.538491594902155
21	29.992487913848382	25.457289082253116	18.5807701495219	25.969452854376602
22	29.005993317870626	27.679753959914365	19.605943437768936	23.708309284446074
23	24.051512199669258	27.697740115648916	22.98779177933073	25.2629559053511
24	24.180084081989055	31.31323933295983	19.721058593332266	24.78561799171885
25	27.53092181021542	24.750660573049824	24.14770148394242	23.57071613279233
26	25.653445307333033	26.660576056912184	18.795156856380828	28.890821779373958
27	26.918114502085132	31.480114021326656	17.945898320145677	23.65587315644253
28	25.647825808310433	31.65926139484987	17.65274465541476	25.040168141424935
29	26.469531884454835	29.45621104056771	17.685221225016956	26.3890358499605
30	28.742158778475023	29.27313565602201	18.25615480798886	23.728550757514107
31	26.049516619715344	29.238478946330776	18.31077107608144	26.40123335787244
32	25.65049460048839	33.48896275888459	17.743953447243147	23.116589193383877
33	26.833220598790508	33.28378526447073	18.145211989492477	21.737782147246286
34	25.44405788723	35.0095916766762	16.459850934800844	23.086499501292952
35	25.374969999580888	34.63385580891749	18.114802794112645	21.876371397388983
36	26.917475495507315	32.63603933198252	18.47971313867063	21.966772033839533
37	25.732588151427265	33.441789037992514	18.863248645540985	21.962374165039236
38	24.702227633313218	35.02626223063289	19.332260679350448	20.93924945670345
39	26.6241714762875	33.67513920476383	19.699031660708556	20.001657658240113
40	26.17459276016824	34.58743386046991	18.137844619536423	21.10012875982543
41	26.41910674774031	35.69611027298925	16.75666949019867	21.128113489071765
42	26.744661804828972	35.824343857709025	16.756443958465326	20.67455037899668
43	27.322135808067983	33.635971860405625	17.37340480935145	21.668487522174935
44	26.57229917761733	32.857605465686376	18.582781140810926	21.987314215885366
45	25.5110032234123	31.92694876802351	20.881870424877352	21.680177583686838
46	25.783539528852934	30.77557168065684	20.70802304708783	22.732865743402396
47	27.472828594567055	29.98183153944766	19.952021882592312	22.59331798339297
48	27.040390290182287	30.739016745543257	19.618949101058703	22.601643863215756
49	26.815591534967098	30.167575716171317	20.306952447949627	22.709880300911955
50	25.85255223925759	32.20038720039754	19.74626176453397	22.200798795810897
51	24.736997108871122	32.04005293229782	20.571745497211957	22.651204461619106
52	26.262832050840867	31.53965439893068	19.789281942670208	22.408231607558246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	5.5
2	11.0
3	15.0
4	19.0
5	183.5
6	348.0
7	1796.0
8	3244.0
9	5768.5
10	8293.0
11	10964.0
12	13635.0
13	15701.5
14	15662.0
15	13556.0
16	16434.5
17	19313.0
18	25185.5
19	31058.0
20	45078.5
21	59099.0
22	81831.5
23	104564.0
24	128585.5
25	152607.0
26	157433.5
27	162260.0
28	148149.0
29	134038.0
30	120418.5
31	106799.0
32	102304.0
33	97809.0
34	98769.0
35	99729.0
36	99399.5
37	99070.0
38	108442.5
39	139282.5
40	160750.0
41	180574.0
42	200398.0
43	207316.5
44	214235.0
45	227701.0
46	241167.0
47	249626.0
48	258085.0
49	282953.5
50	307822.0
51	315352.0
52	322882.0
53	336452.5
54	350023.0
55	384109.5
56	418196.0
57	400646.0
58	383096.0
59	369671.0
60	356246.0
61	307526.0
62	258806.0
63	231516.5
64	183198.5
65	162170.0
66	135277.0
67	108384.0
68	88780.5
69	69177.0
70	50929.5
71	32682.0
72	25171.5
73	17661.0
74	13352.5
75	9044.0
76	6656.5
77	4269.0
78	2253.0
79	237.0
80	158.0
81	79.0
82	70.0
83	61.0
84	41.5
85	22.0
86	13.5
87	5.0
88	2.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5320759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.773178620683426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.47641005738376	12.851419692071614
2	7.500693261106757	2.3661954917278667
3	2.6614790941056907	1.2593995543963132
4	1.474638011303648	0.9303891501256729
5	0.9566081089789836	0.7544375286459851
6	0.7157362866317243	0.6773661776608114
7	0.5311884912512826	0.5864971667630472
8	0.42981274941052444	0.5423610615919396
9	0.3319728363374183	0.4712640160288504
>10	2.8916646991051937	9.489021327576017
>50	0.29344737845776037	3.1515729585236136
>100	0.37864321702724757	15.65385766142892
>500	0.25555721341975607	28.61562119639533
>1k	0.10202477900091039	22.522364386686437
>5k	1.2381647937003687E-4	0.12823263037759405
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAACAAAGTTGGGACCTGCTGGGATTTATTCAAGCCAAACTTTAGTGATGAG	6566	0.12340344676389214	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.00875814897836944	0.0	0.0	0.0	0.0
12	0.020617359290281707	0.0	0.0	0.0	0.0
13	0.048808825958852865	0.0	0.0	0.0	0.0
14	0.21158635450318272	0.0	0.0	0.0	0.0
15	0.4006383299826209	0.0	0.0	0.0	0.0
16	0.4897045703441934	0.0	0.0	0.0	0.0
17	0.5502598407482843	0.0	0.0	0.0	0.0
18	0.6490803285771823	0.0	0.0	0.0	0.0
19	0.7297831004937454	0.0	0.0	0.0	0.0
20	0.803851480587638	0.0	0.0	0.0	0.0
21	0.8840843947263914	0.0	0.0	0.0	0.0
22	0.949507391708589	0.0	0.0	0.0	0.0
23	1.0114910297572208	0.0	0.0	0.0	0.0
24	1.0965916704740808	0.0	0.0	0.0	0.0
25	1.1803015321686248	0.0	0.0	0.0	0.0
26	1.2781635101307915	0.0	0.0	0.0	0.0
27	1.4064346834727903	0.0	0.0	0.0	0.0
28	1.5194636705026483	0.0	0.0	0.0	0.0
29	1.634560031754868	0.0	0.0	0.0	0.0
30	1.7654436143414878	0.0	0.0	0.0	0.0
31	1.892756277816755	0.0	0.0	0.0	0.0
32	2.057018556938963	0.0	0.0	0.0	0.0
33	2.1734869029023867	0.0	0.0	0.0	0.0
34	2.2722885964201724	0.0	0.0	0.0	0.0
35	2.3631215020263086	0.0	0.0	0.0	0.0
36	2.4585778081660905	0.0	0.0	0.0	0.0
37	2.552962838572467	0.0	0.0	0.0	0.0
38	2.647460634845517	0.0	0.0	0.0	0.0
39	2.7444392801854023	0.0	0.0	0.0	0.0
40	2.84846579219243	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTACG	35	1.0205076E-7	46.000004	46
CCAATAC	70	0.0	46.000004	26
CTGTTAG	35	1.0205076E-7	46.000004	36
TAAGACG	35	1.0205076E-7	46.000004	17
TACACTA	35	1.0205076E-7	46.000004	34
ACGACTA	105	0.0	46.000004	26
AACGTTC	40	5.6188583E-9	46.0	29
TGATAGC	80	0.0	46.0	23
CTACTTA	20	6.3135306E-4	46.0	32
ATAGGGT	75	0.0	46.0	19
TTTACGT	25	3.41884E-5	46.0	21
TAGACCC	80	0.0	46.0	33
GTTTACG	60	0.0	46.0	18
ATTACCC	55	1.8189894E-12	46.0	33
TTCACGG	80	0.0	46.0	21
CGCGCAT	20	6.3135306E-4	46.0	45
CGATTTA	100	0.0	46.0	34
TACGGTC	160	0.0	46.0	27
TAACGTA	50	1.6370905E-11	46.0	27
CGATTAG	55	1.8189894E-12	46.0	41
>>END_MODULE