FastQCFastQC Report
Fri 17 Jun 2016
SRR1528105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4803932
Sequences flagged as poor quality0
Sequence length59
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAAAGTTGGGACCTGCTGGGATTTATTCAAGCCAAACTTTAGTGATGAGGCTTGAG51330.10684997206455045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA600.053.00000440
CTAGTTT150.00669755653.00000432
CGTAACG307.0151873E-753.00000427
CGATTTA401.6025297E-953.034
TAACCTA203.139251E-453.019
CGTACTT401.6025297E-953.026
TCCGTAC503.6379788E-1253.046
CGTAATT1250.053.052
TACTAAG700.053.021
ATAACGT353.3451215E-853.035
ACGTAGT850.053.029
CACGTTA1150.053.015
TAAGACG203.139251E-453.017
TAGTTAT1900.051.60526330
CGACGTA1250.050.8846
TTGGGAC220900.050.7566769
ATTTACG2300.050.69565228
CGCAATT2000.050.3540
TATCGCT2000.050.3534
CTAGTCA950.050.2105342
Produced by FastQC (version 0.11.5)