##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528105_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4803932
Sequences flagged as poor quality	0
Sequence length	59
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.385902215102128	31.0	30.0	31.0	28.0	33.0
2	31.163421755345414	31.0	31.0	33.0	30.0	34.0
3	31.604987331211184	31.0	31.0	34.0	30.0	34.0
4	35.48036087937964	37.0	35.0	37.0	33.0	37.0
5	35.080524453718326	35.0	35.0	37.0	33.0	37.0
6	35.48482971865547	37.0	35.0	37.0	33.0	37.0
7	35.15122986753351	37.0	35.0	37.0	32.0	37.0
8	35.28088407579458	37.0	35.0	37.0	32.0	37.0
9	35.22529044957339	37.0	34.0	39.0	30.0	39.0
10	36.73557327622456	37.0	35.0	39.0	33.0	39.0
11	36.087253316658106	37.0	35.0	39.0	32.0	39.0
12	36.82073434844623	39.0	37.0	39.0	33.0	39.0
13	36.69938292215627	39.0	37.0	39.0	32.0	39.0
14	37.006046088912164	39.0	36.0	40.0	31.0	40.0
15	36.269210305224966	38.0	36.0	40.0	30.0	40.0
16	35.94061323099494	38.0	35.0	40.0	30.0	40.0
17	36.088026433346684	38.0	35.0	40.0	30.0	40.0
18	36.265505839799566	38.0	35.0	40.0	30.0	40.0
19	36.53470719402356	39.0	35.0	40.0	30.0	40.0
20	36.49789942905104	39.0	35.0	40.0	30.0	40.0
21	36.40032206950473	39.0	35.0	40.0	30.0	40.0
22	36.374748643402945	39.0	35.0	40.0	30.0	40.0
23	36.29667010274084	38.0	35.0	40.0	30.0	40.0
24	36.31312724659716	38.0	35.0	40.0	30.0	40.0
25	36.35852360108345	39.0	35.0	40.0	30.0	40.0
26	35.98317274266164	38.0	35.0	40.0	29.0	40.0
27	35.7563362678739	38.0	35.0	40.0	27.0	40.0
28	35.60090734007059	38.0	34.0	40.0	27.0	40.0
29	35.527844274232024	38.0	34.0	40.0	27.0	40.0
30	35.34074608050239	38.0	34.0	40.0	27.0	40.0
31	35.36163625963065	38.0	34.0	40.0	27.0	40.0
32	35.3161730848813	38.0	34.0	40.0	27.0	40.0
33	35.076560201101934	38.0	33.0	40.0	26.0	40.0
34	34.92240647869287	38.0	33.0	40.0	25.0	40.0
35	34.811787718893605	38.0	33.0	40.0	25.0	40.0
36	34.53290825099106	37.0	33.0	40.0	25.0	40.0
37	34.51304951860268	37.0	33.0	40.0	25.0	40.0
38	34.40755073135923	37.0	33.0	40.0	24.0	40.0
39	34.086875084826346	37.0	33.0	40.0	23.0	40.0
40	33.87688543468143	36.0	33.0	39.0	23.0	40.0
41	33.51859268615792	36.0	32.0	39.0	22.0	40.0
42	33.30416521299635	36.0	32.0	39.0	21.0	40.0
43	33.02226426185883	35.0	32.0	39.0	21.0	40.0
44	32.72058784345823	35.0	31.0	39.0	20.0	40.0
45	32.51068541353208	35.0	31.0	39.0	20.0	40.0
46	32.288139798814804	35.0	31.0	38.0	18.0	40.0
47	31.86349869232121	35.0	30.0	38.0	15.0	40.0
48	31.609454921510128	35.0	30.0	38.0	15.0	40.0
49	31.31124607925341	35.0	29.0	38.0	13.0	40.0
50	30.859621243597953	34.0	28.0	38.0	12.0	40.0
51	30.573876357950112	34.0	28.0	37.0	10.0	40.0
52	29.875977636652642	34.0	26.0	37.0	10.0	39.0
53	29.760716013465636	34.0	26.0	37.0	10.0	39.0
54	29.62556630693357	34.0	26.0	36.0	9.0	39.0
55	29.38225874138102	33.0	26.0	36.0	9.0	39.0
56	29.05488566449317	33.0	25.0	36.0	9.0	39.0
57	28.6121067075887	33.0	23.0	35.0	8.0	39.0
58	28.309383646562857	33.0	23.0	35.0	8.0	38.0
59	27.521794230226405	32.0	22.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	13.0
14	59.0
15	244.0
16	825.0
17	2262.0
18	5018.0
19	9596.0
20	16538.0
21	25344.0
22	36476.0
23	51163.0
24	67906.0
25	88006.0
26	112015.0
27	137209.0
28	157852.0
29	173806.0
30	196730.0
31	235680.0
32	282638.0
33	339373.0
34	413665.0
35	490965.0
36	624034.0
37	723284.0
38	578449.0
39	34782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.54435574858262	16.880942527912552	32.63310138444923	13.94160033905559
2	32.31852574099717	18.48814679308533	30.53402920774066	18.659298258176843
3	19.284889960973636	24.135312489852062	35.864683346891674	20.71511420228263
4	17.72679130345725	26.31852407569466	38.4863066338158	17.468377987032287
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.35200997849262	0.3414494626485138	0.2695083943736089	0.03703216448525916
12	99.34245530536235	0.4722589745233696	0.0777279944845181	0.10755772562975496
13	97.94720241668699	1.2248924422743703	0.29763119044982317	0.5302739505888093
14	51.79276059694433	33.65053876699337	5.9121361418105	8.644564494251792
15	28.71093928889918	31.95199265934655	19.463535287343785	19.873532764410488
16	31.035535057532037	20.061170724314998	25.730651474667006	23.17264274348596
17	21.743688295338067	20.566964728060263	27.404405391250336	30.284941585351334
18	21.271679116190654	27.45398977337731	28.677320994551962	22.597010115880074
19	24.505946378924598	24.817066519675965	26.354765221489394	24.322221879910042
20	24.08924605926978	24.00660542239149	27.76186257424127	24.14228594409746
21	29.985187134205898	25.65991775070921	18.70157612555715	25.653318989527747
22	29.039232861747415	27.831014260818016	19.691161323682348	23.438591553752218
23	23.621004627043014	27.959450716621305	23.4828261515775	24.936718504758186
24	23.726543173383803	31.891958503992146	19.80629617571606	24.57520214690799
25	27.2667056902554	25.03137013596362	24.393663357433038	23.308260816347943
26	25.283351221457757	26.924902350824283	18.88546715482234	28.906279272895617
27	26.532806875700988	32.124642896693786	17.957185905212647	23.385364322392572
28	25.310974426782064	32.36121993400406	17.577725912856383	24.750079726357495
29	26.18575783337483	29.950777821168163	17.53078519845826	26.33267914699875
30	28.67929854127827	29.830168287144783	18.06332812371199	23.42720504786496
31	25.652382256867917	29.893137538166652	18.192930291269736	26.2615499136957
32	25.29733976251121	34.52051361259901	17.580869171337145	22.601277453552633
33	26.492693901578956	34.19627921461003	18.050755089788947	21.260271794022064
34	25.01367629683351	36.03575154685787	16.4488589763552	22.501713179953423
35	25.22787583171452	35.531039989741735	17.888700339638447	21.352383838905297
36	26.474562920540922	33.52907576543548	18.639210546693832	21.357150767329763
37	25.331374382485016	34.3588751880751	18.72116424628825	21.588586183151634
38	24.292808474391396	36.069515555174384	19.086073657995158	20.55160231243906
39	26.018270033797315	34.76069186657929	19.582229723484843	19.63880837613855
40	25.63585412949226	35.732874653512994	17.936640235540384	20.69463098145436
41	25.853592432199292	37.02111103987317	16.539305718732074	20.58599080919547
42	26.172123169103976	37.16370256698055	16.431185953506418	20.232988310409056
43	26.675981258685592	34.97539099221221	17.132007697028186	21.21662005207401
44	26.070289920839844	34.126648753562705	18.20731434166845	21.595746983928997
45	24.972189448143727	33.13119336410257	20.646899248365713	21.249717939387985
46	25.23428724636402	31.985402790880467	20.44106785857918	22.33924210417633
47	26.956251670506575	31.189096764900086	19.65741813164716	22.197233432946177
48	26.550146838048498	31.950473070809494	19.23632557663181	22.2630545145102
49	26.41896263310971	31.36799188664619	19.86637196363312	22.346673516610977
50	25.24708925938169	33.48057382993764	19.42206925493533	21.850267655745334
51	24.167681807319504	33.2381682338551	20.388340217971447	22.20580974085395
52	25.114468731031163	33.156672492449935	19.6616022041944	22.067256572324503
53	23.92442274370245	33.22141112738481	20.385571652554617	22.468594476358117
54	24.194056035764035	32.53618077857888	19.95382116149854	23.31594202415854
55	24.740108727600642	32.907230993278006	19.867058900916998	22.485601378204354
56	26.38201373374977	32.224061456323696	20.057257263425043	21.33666754650149
57	26.411239792736453	31.752197991145586	19.198585658581347	22.637976557536618
58	25.21188892765343	31.83192434863774	18.72334995582785	24.23283676788098
59	26.051222207141983	29.355348910017874	20.36885617864699	24.224572704193147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	10.0
4	134.5
5	253.0
6	1359.0
7	2465.0
8	4252.0
9	8091.5
10	10144.0
11	11149.0
12	12154.0
13	10230.5
14	8411.0
15	8515.0
16	9347.0
17	10179.0
18	13937.5
19	26245.5
20	34795.0
21	51851.0
22	68907.0
23	85261.5
24	101616.0
25	111303.5
26	121023.0
27	121055.0
28	118291.5
29	115528.0
30	116186.5
31	109335.5
32	101826.0
33	101973.5
34	102121.0
35	100009.5
36	102113.0
37	106328.0
38	113742.5
39	121157.0
40	126965.5
41	132774.0
42	139932.5
43	157026.0
44	166961.0
45	171144.5
46	175328.0
47	182429.5
48	202417.5
49	215304.0
50	220281.0
51	225258.0
52	237539.0
53	269633.5
54	289447.0
55	309922.5
56	330398.0
57	318538.5
58	274255.5
59	241832.0
60	233019.0
61	224206.0
62	206416.0
63	188626.0
64	159062.0
65	125460.0
66	121422.0
67	98431.5
68	75441.0
69	64161.0
70	39722.5
71	26564.0
72	22291.0
73	18018.0
74	12352.5
75	5113.5
76	3540.0
77	2241.5
78	943.0
79	662.0
80	381.0
81	302.5
82	168.5
83	113.0
84	109.0
85	105.0
86	61.0
87	10.5
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	4803932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.550777700337342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.17299251911626	22.273377533291097
2	5.6680562380650095	2.896464898636188
3	2.042543016871634	1.5656568770239052
4	1.1303537561493782	1.1552567018445634
5	0.7099119391266135	0.906940107171976
6	0.472229757291747	0.723950253122741
7	0.33024398696060436	0.5906593488392454
8	0.2521539272541904	0.5154183153231082
9	0.19060694539355805	0.4383140120902026
>10	1.429766512529572	7.50309219107002
>50	0.13875524244050624	2.4503080531430776
>100	0.2786694919349758	20.226141264155643
>500	0.14731075414733466	25.92658526229511
>1k	0.03631711780968043	12.711378756817624
>5k	8.87949090701233E-5	0.11645642517544069
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAACAAAGTTGGGACCTGCTGGGATTTATTCAAGCCAAACTTTAGTGATGAGGCTTGAG	5133	0.10684997206455045	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.008576307907772217	0.0	0.0	0.0	0.0
12	0.018193429882021644	0.0	0.0	0.0	0.0
13	0.042444397630940656	0.0	0.0	0.0	0.0
14	0.1874297970912161	0.0	0.0	0.0	0.0
15	0.35406412913421753	0.0	0.0	0.0	0.0
16	0.4330411004985083	0.0	0.0	0.0	0.0
17	0.4871842482366528	0.0	0.0	0.0	0.0
18	0.5746958949460567	0.0	0.0	0.0	0.0
19	0.6439308466481207	0.0	0.0	0.0	0.0
20	0.7083988699257192	0.0	0.0	0.0	0.0
21	0.7771758634385333	0.0	0.0	0.0	0.0
22	0.8348161464400412	0.0	0.0	0.0	0.0
23	0.8906870455285378	0.0	0.0	0.0	0.0
24	0.9641268860591699	0.0	0.0	0.0	0.0
25	1.034631630922336	0.0	0.0	0.0	0.0
26	1.1189375703069901	0.0	0.0	0.0	0.0
27	1.2277026402538587	0.0	0.0	0.0	0.0
28	1.3251852857201143	0.0	0.0	0.0	0.0
29	1.4211067100866541	0.0	0.0	0.0	0.0
30	1.5334729967035337	0.0	0.0	0.0	0.0
31	1.6480249928600155	0.0	0.0	0.0	0.0
32	1.7902001943408026	0.0	0.0	0.0	0.0
33	1.8935530311419895	0.0	0.0	0.0	0.0
34	1.9783793775598821	0.0	0.0	0.0	0.0
35	2.059812670121059	0.0	0.0	0.0	0.0
36	2.144347588600338	0.0	0.0	0.0	0.0
37	2.2277792441691515	0.0	0.0	0.0	0.0
38	2.312897018525658	0.0	0.0	0.0	0.0
39	2.4013662141762206	2.0816281329544217E-5	0.0	0.0	0.0
40	2.4979537595453056	2.0816281329544217E-5	0.0	0.0	0.0
41	2.588608664735471	2.0816281329544217E-5	0.0	0.0	0.0
42	2.6754958230049883	2.0816281329544217E-5	0.0	0.0	0.0
43	2.759947476358949	2.0816281329544217E-5	0.0	0.0	2.0816281329544217E-5
44	2.841255871232149	2.0816281329544217E-5	0.0	0.0	2.0816281329544217E-5
45	2.9226267149493372	2.0816281329544217E-5	0.0	0.0	2.0816281329544217E-5
46	3.001603686313628	2.0816281329544217E-5	0.0	0.0	2.0816281329544217E-5
47	3.074231691872408	2.0816281329544217E-5	0.0	0.0	2.0816281329544217E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	60	0.0	53.000004	40
CTAGTTT	15	0.006697556	53.000004	32
CGTAACG	30	7.0151873E-7	53.000004	27
CGATTTA	40	1.6025297E-9	53.0	34
TAACCTA	20	3.139251E-4	53.0	19
CGTACTT	40	1.6025297E-9	53.0	26
TCCGTAC	50	3.6379788E-12	53.0	46
CGTAATT	125	0.0	53.0	52
TACTAAG	70	0.0	53.0	21
ATAACGT	35	3.3451215E-8	53.0	35
ACGTAGT	85	0.0	53.0	29
CACGTTA	115	0.0	53.0	15
TAAGACG	20	3.139251E-4	53.0	17
TAGTTAT	190	0.0	51.605263	30
CGACGTA	125	0.0	50.88	46
TTGGGAC	22090	0.0	50.756676	9
ATTTACG	230	0.0	50.695652	28
CGCAATT	200	0.0	50.35	40
TATCGCT	200	0.0	50.35	34
CTAGTCA	95	0.0	50.21053	42
>>END_MODULE