FastQCFastQC Report
Fri 17 Jun 2016
SRR1528104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3877068
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTATGTAGGGGCCCTTTCCCGTCCTGGGCTGAACGAGGCAGGCTCGTGG40840.10533733223146975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTC1550.046.00000417
CTCGACA351.0204167E-746.00000445
TATAGCG351.0204167E-746.00000441
ATAACCG351.0204167E-746.00000438
CGGGTCA800.046.039
CCCCTTA501.6370905E-1146.022
GGTACTC301.8625542E-646.013
GATACGC1250.046.022
TACCCTC501.6370905E-1146.016
ATCTCGA405.6170393E-946.041
GCAAGCC253.418667E-546.036
CGTATAA253.418667E-546.038
ATAGGCG800.046.019
TTTACGC253.418667E-546.025
ACGTTCA900.046.039
CCGATAC900.046.019
TCCGAAC453.110472E-1046.019
GCGCGAA1700.046.017
TTGCTCG1350.046.032
TAGCGTT900.046.042
Produced by FastQC (version 0.11.5)