##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528104_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3877068
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.797103120192887	31.0	31.0	33.0	30.0	34.0
2	31.61657907470284	31.0	31.0	34.0	30.0	34.0
3	31.931148228506697	31.0	31.0	34.0	30.0	34.0
4	35.64292888337269	37.0	35.0	37.0	33.0	37.0
5	35.130987643239685	35.0	35.0	37.0	32.0	37.0
6	35.485519727794305	37.0	35.0	37.0	33.0	37.0
7	35.80515946586441	37.0	35.0	37.0	35.0	37.0
8	35.800220166373144	37.0	35.0	37.0	33.0	37.0
9	36.29846703746233	37.0	35.0	39.0	32.0	39.0
10	36.72955078425243	39.0	35.0	39.0	32.0	39.0
11	37.548794088728904	39.0	37.0	39.0	35.0	39.0
12	37.59697276395462	39.0	37.0	39.0	35.0	39.0
13	37.499176955369364	39.0	37.0	39.0	35.0	39.0
14	37.924070457366234	40.0	38.0	40.0	33.0	40.0
15	37.25847134999954	39.0	37.0	40.0	32.0	40.0
16	36.78993636428353	38.0	35.0	40.0	31.0	40.0
17	36.5561439727134	39.0	35.0	40.0	31.0	40.0
18	36.72713684670994	39.0	35.0	40.0	31.0	40.0
19	36.843569418952676	39.0	35.0	40.0	31.0	40.0
20	37.00010729757641	39.0	35.0	40.0	32.0	40.0
21	36.914730409680715	39.0	35.0	40.0	32.0	40.0
22	36.99192250432543	39.0	35.0	40.0	32.0	40.0
23	36.978835810978815	39.0	35.0	40.0	32.0	40.0
24	36.92721355416	39.0	35.0	40.0	32.0	40.0
25	36.9818329211662	39.0	35.0	40.0	32.0	40.0
26	36.85152362558511	39.0	35.0	40.0	31.0	40.0
27	36.71315514713696	39.0	35.0	40.0	31.0	40.0
28	36.59410461720042	39.0	35.0	40.0	31.0	40.0
29	36.57113390840708	39.0	35.0	40.0	31.0	40.0
30	36.4382425069666	38.0	35.0	40.0	31.0	40.0
31	36.374930230782645	38.0	35.0	40.0	31.0	40.0
32	36.32930142055801	38.0	35.0	40.0	31.0	40.0
33	36.177651771905985	38.0	35.0	40.0	31.0	40.0
34	35.96260885803396	38.0	35.0	40.0	30.0	40.0
35	35.87803334891212	38.0	35.0	40.0	30.0	40.0
36	35.770298328530735	38.0	35.0	40.0	30.0	40.0
37	35.60560660787998	38.0	35.0	40.0	29.0	40.0
38	35.237845454348495	38.0	34.0	40.0	27.0	40.0
39	35.053387508292346	38.0	34.0	40.0	26.0	40.0
40	34.83129132633216	37.0	34.0	40.0	25.0	40.0
41	34.52843514738457	37.0	34.0	40.0	24.0	40.0
42	34.15960385528446	37.0	33.0	40.0	22.0	40.0
43	33.77876632548101	37.0	33.0	40.0	20.0	40.0
44	33.361543052636684	36.0	33.0	40.0	17.0	40.0
45	33.052336198384964	36.0	33.0	40.0	15.0	40.0
46	32.76135110346272	36.0	33.0	40.0	12.0	40.0
47	32.389580734720155	35.0	32.0	40.0	10.0	40.0
48	32.148128173145274	35.0	32.0	40.0	10.0	40.0
49	31.978882237814762	35.0	31.0	39.0	10.0	40.0
50	31.766727073138775	35.0	31.0	39.0	10.0	40.0
51	31.530047964080072	35.0	31.0	39.0	9.0	40.0
52	30.701485761921123	35.0	29.0	38.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	10.0
15	50.0
16	195.0
17	567.0
18	1327.0
19	2842.0
20	5371.0
21	8917.0
22	13643.0
23	19797.0
24	28068.0
25	39062.0
26	52940.0
27	70419.0
28	91468.0
29	116684.0
30	141627.0
31	157452.0
32	163564.0
33	182538.0
34	244785.0
35	367463.0
36	398095.0
37	584646.0
38	950825.0
39	234706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.065409221607666	20.190308759093213	36.808949443239065	14.935332576060054
2	26.85498423035139	21.92876163121204	34.37102470217185	16.845229436264724
3	27.369909426401602	21.13904115171568	35.480290776432085	16.010758645450636
4	25.812882312097702	21.241386532297085	36.78645822049033	16.159272935114885
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.52954139571449	0.37621212730857445	0.0730448885601181	0.021201588416813945
12	99.00432491769554	0.8153584100149908	0.10706544223624656	0.07325123005322579
13	97.11712562173271	1.8704082569611882	0.4878428750798284	0.524623246226272
14	52.423480836549686	32.971952000841874	6.2181782728598	8.386388889748646
15	34.81218281443606	27.560930063646033	19.483202254899837	18.143684867018067
16	34.49550020788905	23.89372587738982	22.16819514127686	19.442578773444264
17	25.131413738422953	25.07675903543606	25.58157349832399	24.210253727816998
18	21.338083314504672	30.72705972657689	28.833154332088064	19.101702626830377
19	23.120125827042497	31.446159830057148	27.994995187084676	17.438719155815683
20	25.278715771815197	32.810928258157965	24.304500204793932	17.605855765232903
21	27.044895782070366	33.84892913923615	19.41598651352001	19.69018856517348
22	26.634714686458942	33.44932304514649	22.68015417836365	17.235808090030922
23	23.14805930667195	38.021669983606174	20.39497372756939	18.435296982152494
24	24.706505018740966	36.16121770368743	21.64426829758983	17.48800897998178
25	25.46460366441858	35.29497032293475	23.105888264018066	16.134537748628603
26	24.814911680682414	34.00159605144919	22.759440897090276	18.424051370778123
27	25.784252429929005	35.36577124775733	19.789026140372055	19.06095018194161
28	26.064412592195957	34.20899504470904	19.872259140154362	19.854333222940635
29	25.765810658982506	34.70834145802962	17.735954076637295	21.789893806350573
30	24.789454298970252	34.94875509018671	19.54693598358347	20.71485462725957
31	22.559341234149105	36.40983341019554	19.08057841647348	21.950246939181877
32	21.380976552384432	38.858333152784525	19.145292267249374	20.615398027581666
33	27.638540257741155	37.68904749671659	18.237157563395844	16.435254682146404
34	25.27226760015558	37.259289751946575	19.268736065501045	18.1997065823968
35	26.432551608586696	36.511018119878216	19.188830322295093	17.86759994923999
36	28.000489029338667	35.66024635110862	20.459378066105625	15.87988655344709
37	26.25747085168483	38.036346022303455	20.281821211286466	15.424361914725251
38	23.521047348150717	38.86710266624161	23.26830481178045	14.343545173827232
39	24.049307363193012	40.75922836535237	20.068747826966153	15.122716444488463
40	25.688148879514106	40.240073168693456	19.023860298555505	15.047917653236931
41	27.65698202868766	40.202235297394836	17.077312030637586	15.063470643279922
42	25.180419843036027	40.70106585698265	16.89518987028342	17.223324429697907
43	26.617253037604705	36.661673202533464	19.272089114764043	17.44898464509779
44	25.786496393666553	35.35888460042486	18.752933918105125	20.101685087803464
45	22.889513415808025	36.39100474894946	20.622181504167582	20.097300331074923
46	25.448663784076007	35.162060608686765	17.64286827055909	21.74640733667813
47	25.280443881819973	33.061168903924305	19.127701654961946	22.530685559293776
48	25.98520325152925	33.24380691801124	18.447780642485505	22.323209187974005
49	26.42994654723621	35.10247950255193	18.272287202597425	20.195286747614436
50	24.165090733513058	37.927268750509405	17.580682103073766	20.32695841290377
51	22.97787916023139	36.92223092295518	18.88282588801641	21.21706402879702
52	25.90493641071036	32.793776121543395	20.996381801918357	20.30490566582789
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	66.0
2	132.0
3	139.5
4	147.0
5	117.0
6	87.0
7	2562.0
8	5037.0
9	9663.5
10	14290.0
11	18555.5
12	22821.0
13	25850.5
14	29708.5
15	30537.0
16	32697.5
17	34858.0
18	37483.5
19	40109.0
20	48687.0
21	57265.0
22	70444.0
23	83623.0
24	96540.5
25	109458.0
26	115403.0
27	121348.0
28	120898.0
29	120448.0
30	120430.0
31	120412.0
32	116404.5
33	112397.0
34	112028.0
35	111659.0
36	108153.5
37	104648.0
38	121369.0
39	144447.0
40	150804.0
41	163947.5
42	177091.0
43	178700.0
44	180309.0
45	192636.5
46	204964.0
47	208679.0
48	212394.0
49	211656.0
50	210918.0
51	221252.0
52	231586.0
53	233556.5
54	235527.0
55	243518.5
56	251510.0
57	244072.5
58	236635.0
59	225350.0
60	214065.0
61	168800.5
62	123536.0
63	101676.5
64	66498.5
65	53180.0
66	44670.0
67	36160.0
68	25250.5
69	14341.0
70	9225.0
71	4109.0
72	3372.0
73	2635.0
74	1814.5
75	994.0
76	531.5
77	69.0
78	64.0
79	59.0
80	68.0
81	77.0
82	54.5
83	32.0
84	19.5
85	7.0
86	4.0
87	1.0
88	1.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3877068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.043542168720656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.06325085639101	18.750953572613184
2	6.143607289744316	2.7085373271907693
3	2.070531436104507	1.3692554107029433
4	1.1459309709466252	1.0104151072201977
5	0.7605417403228331	0.8382516961939283
6	0.5511829273860397	0.7290014461507838
7	0.4438196155611648	0.684834948764955
8	0.32966474180478356	0.5813582910011323
9	0.28265797475442234	0.5607704687241859
>10	2.4866048988781637	10.80275437988319
>50	0.17765011497735558	2.71167034522535
>100	0.3384121201926748	20.46589248261191
>500	0.15915096427626185	24.419269138081866
>1k	0.046994348659961646	14.36703538563564
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGGTATGTAGGGGCCCTTTCCCGTCCTGGGCTGAACGAGGCAGGCTCGTGG	4084	0.10533733223146975	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.11862056585027655	0.0	0.0	0.0	0.0
11	0.12246367615940705	0.0	0.0	0.0	0.0
12	0.15700008356830472	0.0	0.0	0.0	0.0
13	0.22150759285109262	0.0	0.0	0.0	0.0
14	0.3558359048641912	0.0	0.0	0.0	0.0
15	0.5607845928933927	0.0	0.0	0.0	0.0
16	0.6818296712876845	0.0	0.0	0.0	0.0
17	0.7860321253070619	0.0	0.0	0.0	0.0
18	0.9242293403159295	0.0	0.0	0.0	0.0
19	1.0532701515681437	0.0	0.0	0.0	0.0
20	1.1652104115790591	0.0	0.0	0.0	0.0
21	1.2701350608243136	0.0	0.0	0.0	0.0
22	1.3815594671024598	0.0	0.0	0.0	0.0
23	1.4894760679977757	0.0	0.0	0.0	0.0
24	1.600513583976345	0.0	0.0	0.0	0.0
25	1.72870323656949	0.0	0.0	0.0	0.0
26	1.8720589889060497	0.0	0.0	0.0	0.0
27	2.0144862045236245	0.0	0.0	0.0	0.0
28	2.152399699979469	0.0	0.0	0.0	0.0
29	2.3123659425111964	0.0	0.0	0.0	0.0
30	2.4908255413627	0.0	0.0	0.0	0.0
31	2.6525972719591198	0.0	0.0	0.0	0.0
32	2.7825666199303183	0.0	0.0	0.0	0.0
33	2.9005423686146337	0.0	0.0	0.0	0.0
34	3.016738421920895	0.0	0.0	0.0	0.0
35	3.124603437442934	0.0	0.0	0.0	0.0
36	3.225298086079481	0.0	0.0	0.0	0.0
37	3.327488710541058	0.0	0.0	0.0	0.0
38	3.42508823678099	0.0	0.0	0.0	0.0
39	3.5213207506290836	0.0	0.0	0.0	0.0
40	3.6176306425370925	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCTC	155	0.0	46.000004	17
CTCGACA	35	1.0204167E-7	46.000004	45
TATAGCG	35	1.0204167E-7	46.000004	41
ATAACCG	35	1.0204167E-7	46.000004	38
CGGGTCA	80	0.0	46.0	39
CCCCTTA	50	1.6370905E-11	46.0	22
GGTACTC	30	1.8625542E-6	46.0	13
GATACGC	125	0.0	46.0	22
TACCCTC	50	1.6370905E-11	46.0	16
ATCTCGA	40	5.6170393E-9	46.0	41
GCAAGCC	25	3.418667E-5	46.0	36
CGTATAA	25	3.418667E-5	46.0	38
ATAGGCG	80	0.0	46.0	19
TTTACGC	25	3.418667E-5	46.0	25
ACGTTCA	90	0.0	46.0	39
CCGATAC	90	0.0	46.0	19
TCCGAAC	45	3.110472E-10	46.0	19
GCGCGAA	170	0.0	46.0	17
TTGCTCG	135	0.0	46.0	32
TAGCGTT	90	0.0	46.0	42
>>END_MODULE