FastQCFastQC Report
Fri 17 Jun 2016
SRR1528103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850759
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTATGTAGGGGCCCTTTCCCGTCCTGGGCTGAACGAGGCAGGCTCGTGG40110.10416128352877965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA405.6170393E-946.00000424
CTAGACG405.6170393E-946.00000440
TTAGGCG551.8189894E-1246.00000429
GATACTA405.6170393E-946.00000416
TACCCTC405.6170393E-946.00000416
CGAACTT206.313311E-446.00000443
CGACGTA1600.046.00000417
AGATACT405.6170393E-946.00000415
TAGAACG206.313311E-446.00000416
CCTACCG1600.046.00000420
GTTAGGC551.8189894E-1246.00000428
TTATCGC405.6170393E-946.00000418
TCGATAC206.313311E-446.00000439
ATACTAC405.6170393E-946.00000446
CGCTCTA1100.046.00000446
CTAGTAA405.6170393E-946.00000443
TAATTCG405.6170393E-946.00000425
TAACAGG206.313311E-446.00000428
ACGCTTG800.046.00000443
TTACGTG800.046.00000439
Produced by FastQC (version 0.11.5)