FastQCFastQC Report
Fri 17 Jun 2016
SRR1528102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523881
Sequences flagged as poor quality0
Sequence length59
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA750.053.00000439
CTTAACG1150.053.020
CGACGTA1300.053.017
CTATACG650.053.032
ATTACGC401.6025297E-953.015
ATTCGTT503.6379788E-1253.027
CTAAGTT1100.053.029
CGATTAC353.3447577E-853.053
CGTTAGA2200.053.045
ACGATAA150.006697409353.019
CCGTACA1250.053.050
TACGCAC503.6379788E-1253.043
ACGGACC401.6025297E-953.051
TACCTAA150.006697409353.014
TTAACGA353.3447577E-853.031
ATGCGCG150.006697409353.014
TACTAGC503.6379788E-1253.028
TACTAAT251.4793615E-553.019
TACTAAC251.4793615E-553.026
CCGAACA353.3447577E-853.020
Produced by FastQC (version 0.11.5)