##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528102_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3523881
Sequences flagged as poor quality	0
Sequence length	59
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.39779918788404	31.0	30.0	31.0	28.0	33.0
2	31.205105109962567	31.0	31.0	33.0	30.0	34.0
3	31.589108712808407	31.0	31.0	34.0	30.0	34.0
4	35.397590894811714	37.0	35.0	37.0	33.0	37.0
5	34.75711239965254	35.0	35.0	37.0	32.0	37.0
6	35.12264432311988	35.0	35.0	37.0	33.0	37.0
7	35.39103193325768	37.0	35.0	37.0	33.0	37.0
8	35.36414935691642	37.0	35.0	37.0	33.0	37.0
9	35.7414512578603	37.0	35.0	39.0	32.0	39.0
10	36.25455768795825	37.0	35.0	39.0	32.0	39.0
11	36.899778397738174	38.0	37.0	39.0	34.0	39.0
12	37.12052819036738	39.0	37.0	39.0	34.0	39.0
13	36.998676743056876	39.0	37.0	39.0	33.0	39.0
14	37.07932844497303	40.0	36.0	40.0	31.0	40.0
15	36.42474192516717	38.0	36.0	40.0	31.0	40.0
16	35.74906786012354	38.0	34.0	40.0	30.0	40.0
17	35.703013240231435	38.0	34.0	40.0	27.0	40.0
18	35.90994985358473	38.0	35.0	40.0	29.0	40.0
19	36.16526352620875	38.0	35.0	40.0	30.0	40.0
20	36.22401948306427	38.0	35.0	40.0	30.0	40.0
21	36.128753496500025	38.0	35.0	40.0	30.0	40.0
22	36.100918561097835	38.0	35.0	40.0	30.0	40.0
23	36.073179542669	38.0	34.0	40.0	30.0	40.0
24	36.089821705102985	38.0	35.0	40.0	30.0	40.0
25	36.062084673120346	38.0	35.0	40.0	30.0	40.0
26	35.7522370931368	38.0	34.0	40.0	29.0	40.0
27	35.59031306675793	38.0	34.0	40.0	27.0	40.0
28	35.4629685281654	38.0	34.0	40.0	27.0	40.0
29	35.36825676008923	38.0	34.0	40.0	27.0	40.0
30	35.15408806370022	37.0	34.0	40.0	27.0	40.0
31	35.11192971612832	37.0	34.0	40.0	27.0	40.0
32	35.08015452281164	37.0	34.0	40.0	27.0	40.0
33	34.80809227099326	37.0	33.0	40.0	26.0	40.0
34	34.541728281970926	37.0	33.0	40.0	25.0	40.0
35	34.425068553677036	37.0	33.0	40.0	24.0	40.0
36	34.15977894826755	37.0	33.0	40.0	23.0	40.0
37	34.09938133552183	36.0	33.0	40.0	23.0	40.0
38	33.80438385972739	36.0	33.0	40.0	22.0	40.0
39	33.562006776051746	36.0	33.0	40.0	21.0	40.0
40	33.4005155111651	36.0	32.0	39.0	21.0	40.0
41	33.0440491605704	35.0	32.0	39.0	19.0	40.0
42	32.767310246855665	35.0	31.0	39.0	18.0	40.0
43	32.43968539232738	35.0	31.0	39.0	15.0	40.0
44	32.03283936092053	35.0	31.0	39.0	14.0	40.0
45	31.771961368729535	35.0	30.0	39.0	10.0	40.0
46	31.456178855074846	35.0	30.0	39.0	10.0	40.0
47	31.06414717182561	35.0	29.0	38.0	10.0	40.0
48	30.82851662698031	35.0	28.0	38.0	9.0	40.0
49	30.51718176635363	35.0	27.0	38.0	9.0	40.0
50	30.11670115988593	35.0	26.0	38.0	9.0	40.0
51	29.899001697276383	34.0	26.0	38.0	9.0	40.0
52	29.31844491911049	34.0	24.0	37.0	8.0	40.0
53	29.23639390773979	34.0	23.0	37.0	8.0	40.0
54	29.1595232642646	34.0	24.0	37.0	8.0	40.0
55	29.022128726821364	34.0	23.0	37.0	8.0	40.0
56	28.74637310397258	34.0	23.0	37.0	8.0	39.0
57	28.424341514370095	33.0	22.0	36.0	8.0	39.0
58	28.190148305235052	33.0	21.0	36.0	8.0	39.0
59	27.400382986826173	33.0	18.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	12.0
14	58.0
15	249.0
16	829.0
17	2320.0
18	5250.0
19	9779.0
20	16218.0
21	24431.0
22	34687.0
23	48198.0
24	63852.0
25	82402.0
26	103087.0
27	123898.0
28	134651.0
29	131696.0
30	135598.0
31	155097.0
32	186108.0
33	226149.0
34	292975.0
35	345631.0
36	394196.0
37	469367.0
38	491867.0
39	45271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.492931514997245	20.54924669703659	35.96219622626303	14.995625561703132
2	26.402083384768098	22.462024114889235	34.03801093169718	17.09788156864548
3	27.39471054783065	21.303925983879704	35.297786730028626	16.003576738261025
4	25.912878442830507	21.438578658019384	36.37705132494542	16.271491574204692
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.49061844029353	0.40903764911471185	0.07477551029674384	0.02556840029501564
12	99.03390608252663	0.7937555212562514	0.09591697335977009	0.07642142285735529
13	97.06539466003534	1.9056262115548168	0.5008965966784916	0.5280825317313496
14	52.0946649447016	33.17614868379494	6.354045440240462	8.375140931263001
15	34.987532212353365	27.781131088138334	19.397788971875045	17.833547727633253
16	34.841868950739254	23.78170545486638	22.015158854683232	19.36126673971113
17	25.22664641626661	24.947266948004206	25.444502808125474	24.381583827603713
18	21.312155546682764	30.858647042848496	28.578689234965655	19.25050817550309
19	23.348433162186804	31.543346668062856	27.877814262172873	17.23040590757747
20	25.34620209933309	32.818645124509025	24.42809504634237	17.407057729815506
21	27.09733955261259	33.83556368674198	19.559655958870348	19.50744080177509
22	26.792420061857936	33.37547437044554	22.837348934314182	16.99475663338234
23	23.035794909078938	38.20971820558072	20.624703274599796	18.129783610740542
24	24.63414627224926	36.324240233992015	21.740773879708197	17.30083961405053
25	25.42180056591014	35.45196333247348	23.16585037917001	15.960385722446361
26	24.5735596633371	34.126209142703736	23.074133320620078	18.226097873339082
27	25.794486249677558	35.54680194932803	19.85038654824042	18.808325252753992
28	26.077952121538722	34.33997913096384	19.89891259097569	19.683156156521743
29	25.714063556629753	34.902512315256956	17.673241519790253	21.710182608323038
30	24.707729914829702	35.09962453329156	19.714712273201055	20.477933278677686
31	22.372236746927605	36.74125204568486	19.039462456308826	21.847048751078713
32	21.183518966730148	39.39968460910002	19.00271320172276	20.41408322244707
33	27.565573298303775	38.14169661234304	18.21122790468804	16.081502184665148
34	25.138788738893282	37.82826378075764	19.312627185764786	17.720320294584297
35	26.338800884592867	36.83648795177817	19.329966023256745	17.49474514037222
36	28.01507769416731	36.044718876715756	20.553560123057505	15.386643306059428
37	26.163426063479445	38.53929800694178	20.301678745678416	14.995597183900363
38	23.333449682324687	39.53975744356861	23.200414542942852	13.92637833116385
39	23.765388218274115	41.51116340194235	20.015715627173563	14.707732752609978
40	25.275314348015726	41.30233115136408	18.76198430083195	14.660370199788245
41	27.376009575805764	41.058055025127125	16.939590184799087	14.626345214268019
42	24.775552863448	41.75980970980575	16.619148035929705	16.845489390816546
43	26.169952958116347	37.55597876318752	19.217334524065937	17.056733754630194
44	25.445269008800242	36.129398240178936	18.524263447034674	19.901069303986144
45	22.231028800348252	37.37402029183165	20.47265500736262	19.922295900457478
46	24.903763776359078	36.15141941512781	17.262756602734314	21.6820602057788
47	24.831230112481098	33.791095669802694	18.83156667322194	22.54610754449427
48	25.482784464061076	34.08931799910383	18.11267179567074	22.315225741164358
49	26.08550061707532	35.90637708821609	18.048878495045663	19.95924379966293
50	23.50817181397442	38.81266138101712	17.46219580059599	20.216971004412464
51	22.454021574508335	37.603908872064636	18.7996700229094	21.14239953051763
52	24.496826084649285	34.16593806658057	21.203014517232564	20.134221331537585
53	24.52063506117261	34.207085880595855	21.259571478151503	20.01270758008003
54	22.107102935655316	36.07670066043661	20.882657501771483	20.933538902136593
55	21.720114839292247	33.80690210594512	22.876907591374398	21.596075463388235
56	22.288550606561344	33.853157924458856	21.82880182389814	22.02948964508166
57	20.798687583377532	32.64965530901867	23.955860030460734	22.59579707714307
58	21.100230115602656	33.36471918319603	22.5946619650323	22.940388736169012
59	23.68119127745801	30.954592394011033	21.863820032515285	23.500396296015673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	46.5
2	93.0
3	93.0
4	74.0
5	55.0
6	1914.0
7	3773.0
8	7052.5
9	13402.0
10	16472.0
11	18243.0
12	20014.0
13	19861.0
14	19758.0
15	19808.0
16	18729.5
17	17651.0
18	19055.5
19	25470.0
20	30480.0
21	36887.0
22	43294.0
23	52545.0
24	61796.0
25	72945.0
26	91554.5
27	99015.0
28	104782.0
29	110549.0
30	115575.5
31	117986.5
32	115371.0
33	114320.5
34	113270.0
35	115094.5
36	114861.5
37	112804.0
38	113177.5
39	113551.0
40	120234.5
41	126918.0
42	134950.0
43	140829.0
44	138676.0
45	147167.5
46	155659.0
47	160474.5
48	157155.0
49	149020.0
50	161059.5
51	173099.0
52	172418.0
53	180913.5
54	190090.0
55	199086.0
56	208082.0
57	200935.0
58	173494.0
59	153200.0
60	132511.0
61	111822.0
62	92349.0
63	72876.0
64	58562.0
65	37931.0
66	31614.0
67	26279.5
68	20945.0
69	16318.5
70	9156.0
71	6620.0
72	4635.0
73	2650.0
74	2194.5
75	1097.0
76	455.0
77	358.0
78	261.0
79	183.5
80	106.0
81	77.5
82	43.0
83	37.0
84	26.0
85	15.0
86	11.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	3523881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.667204253012297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.60498086474517	27.47934250266399
2	4.442103733176015	2.724538049967547
3	1.517110479255876	1.39576610825176
4	0.8546919954236275	1.0484405598828417
5	0.5352948945642857	0.8207998933598817
6	0.4178457722825874	0.7688496986908663
7	0.29576387619595423	0.634917584137478
8	0.24061751492661082	0.5903253181685288
9	0.1755894566162402	0.48463539576531117
>10	1.4019476847634251	8.222375284930012
>50	0.10698685340013556	2.3318604270138428
>100	0.2870657386297456	24.135725732236445
>500	0.09832869005011154	20.44932128602148
>1k	0.021672445970228667	8.913102158910101
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.10698431643974357	0.0	0.0	0.0	0.0
11	0.11101396443296467	0.0	0.0	0.0	0.0
12	0.1419173916485829	0.0	0.0	0.0	0.0
13	0.19813381893429433	0.0	0.0	0.0	0.0
14	0.3107653181251013	0.0	0.0	0.0	0.0
15	0.4844374710723773	0.0	0.0	0.0	0.0
16	0.5952527908859578	0.0	0.0	0.0	0.0
17	0.6827699346260557	0.0	0.0	0.0	0.0
18	0.8005661939208504	0.0	0.0	0.0	0.0
19	0.909309934132282	0.0	0.0	0.0	0.0
20	1.003978284170209	0.0	0.0	0.0	0.0
21	1.0961210097616805	0.0	0.0	0.0	0.0
22	1.1920096053186813	0.0	0.0	0.0	0.0
23	1.282080751308004	0.0	0.0	0.0	0.0
24	1.3766639679376234	0.0	0.0	0.0	0.0
25	1.4817185937890638	0.0	0.0	0.0	0.0
26	1.6006215873918557	0.0	0.0	0.0	0.0
27	1.7212840047663358	0.0	0.0	0.0	0.0
28	1.8379451519503638	0.0	0.0	0.0	0.0
29	1.9773369191524912	0.0	0.0	0.0	0.0
30	2.1306338097115085	0.0	0.0	0.0	0.0
31	2.2633284154601134	0.0	0.0	0.0	0.0
32	2.376101803664766	0.0	0.0	0.0	0.0
33	2.473182266938072	0.0	0.0	0.0	0.0
34	2.5701775968030702	0.0	0.0	0.0	0.0
35	2.6614689883114666	0.0	0.0	0.0	0.0
36	2.745921329352495	0.0	0.0	0.0	0.0
37	2.8349141188365894	0.0	0.0	0.0	0.0
38	2.9219488399296116	0.0	0.0	0.0	0.0
39	3.0062592919567943	0.0	0.0	0.0	0.0
40	3.0915062114753593	0.0	0.0	0.0	0.0
41	3.174284262153007	0.0	0.0	0.0	0.0
42	3.2579136469137295	0.0	0.0	0.0	0.0
43	3.3434159666572167	0.0	0.0	0.0	0.0
44	3.4282088413314753	0.0	0.0	0.0	0.0
45	3.520408322528485	0.0	0.0	0.0	0.0
46	3.611472691614728	0.0	0.0	0.0	0.0
47	3.694477764714529	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTA	75	0.0	53.000004	39
CTTAACG	115	0.0	53.0	20
CGACGTA	130	0.0	53.0	17
CTATACG	65	0.0	53.0	32
ATTACGC	40	1.6025297E-9	53.0	15
ATTCGTT	50	3.6379788E-12	53.0	27
CTAAGTT	110	0.0	53.0	29
CGATTAC	35	3.3447577E-8	53.0	53
CGTTAGA	220	0.0	53.0	45
ACGATAA	15	0.0066974093	53.0	19
CCGTACA	125	0.0	53.0	50
TACGCAC	50	3.6379788E-12	53.0	43
ACGGACC	40	1.6025297E-9	53.0	51
TACCTAA	15	0.0066974093	53.0	14
TTAACGA	35	3.3447577E-8	53.0	31
ATGCGCG	15	0.0066974093	53.0	14
TACTAGC	50	3.6379788E-12	53.0	28
TACTAAT	25	1.4793615E-5	53.0	19
TACTAAC	25	1.4793615E-5	53.0	26
CCGAACA	35	3.3447577E-8	53.0	20
>>END_MODULE