FastQCFastQC Report
Fri 17 Jun 2016
SRR1528101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36670550
Sequences flagged as poor quality0
Sequence length52
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4433481.2090028646966025No Hit
CCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3079220.8396983410393353No Hit
AAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2163740.5900484175994087No Hit
GGGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2045510.5578072867737189No Hit
GCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1539650.41986007845532725No Hit
GCCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1261890.344115373235471No Hit
TCCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1119840.3053785667245242No Hit
TCGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1086770.29636043091799824No Hit
GGAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1078130.2940043168155373No Hit
GAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050770.2865432888244109No Hit
TAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA876510.23902286712361828No Hit
CCGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA771550.21040044395298135No Hit
CCAAAATAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA502500.13703094172299024No Hit
ACAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA498420.135918332285717No Hit
GTCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA496700.13544929105235673No Hit
TCGCAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA453620.12370144434703052No Hit
CGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA418790.11420335937148475No Hit
GGCAAATAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA411810.11229992459889475No Hit
TAAAAATAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA389820.10630328697006182No Hit
CTCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380280.10370174431526115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGT405.6188583E-946.015
TACGACC405.6188583E-946.034
GGCAAAT1203800.043.753111
CCAAAAT640500.042.9117851
TTACCGG1400.042.71428339
GCAAATA1771200.042.702972
GCCAAAT456600.042.6552731
CCAAATA1085750.042.5830962
TACGGTT650.042.46153637
AGGGAAA5788600.042.45340710
CCTAACT5650.042.33628546
TAACTCC1850.042.2702730
CCGAAAT187550.042.11251
TTAGCAC2250.041.9111122
CGAACTA1100.041.8181824
TAAAAGG568400.041.5691387
CCTAAGT1550.041.54838624
ATAAAAG642650.041.4261256
CAAATAA4326400.041.323873
TAAAGGA1646500.041.3008167
Produced by FastQC (version 0.11.5)