FastQCFastQC Report
Fri 17 Jun 2016
SRR1528100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36650846
Sequences flagged as poor quality0
Sequence length52
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4398061.199988671475687No Hit
CCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3053140.8330339768964677No Hit
AAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2138640.5835172263145031No Hit
GGGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2027260.553127750448107No Hit
GCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1519350.41454704756337685No Hit
GCCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1246100.33999215188648035No Hit
TCCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1103140.3009862309863188No Hit
TCGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1079010.29440248118692813No Hit
GGAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1064410.29041894421754955No Hit
GAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1041580.2841898929154323No Hit
TAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA872280.23799723477051526No Hit
CCGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA766190.20905110894302412No Hit
GTCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA495290.1351373990111988No Hit
CCAAAATAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA493450.13463536421505795No Hit
ACAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA493300.13459443746537256No Hit
TCGCAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA446650.12186621831321437No Hit
CGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA419110.11435206707097567No Hit
GGCAAATAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA407080.11106974174620689No Hit
TAAAAATAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA388550.10601392393507098No Hit
CTCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA377570.10301808585809998No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTT700.046.00000437
CGATCTA253.4192366E-546.044
CGTACTG1800.044.72221828
CTAACAT1750.044.68571532
GGCAAAT1205400.043.8247871
CTACCCG1050.043.80952528
ACGTACT1800.043.44444327
CCAAAAT652250.042.8545841
CGTCGCT1450.042.82758322
CCTATCT1400.042.71428731
CGCCCTA2050.042.63414421
GCAAATA1775450.042.591682
GCCAAAT454250.042.567091
CGGTACT650.042.4615427
AGGGAAA5776000.042.43890410
CCAAATA1089900.042.393522
CACGACC601.8189894E-1242.16666836
CCGAAAT179900.042.1389661
CCCGCCG1150.042.042
CGAACTA1100.041.8181824
Produced by FastQC (version 0.11.5)