##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528100_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36650846
Sequences flagged as poor quality	0
Sequence length	52
%GC	30
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.25649514338632	31.0	30.0	34.0	16.0	34.0
2	31.024877734063764	31.0	30.0	34.0	27.0	34.0
3	31.60970226990122	31.0	31.0	34.0	30.0	34.0
4	35.37247189328181	37.0	35.0	37.0	33.0	37.0
5	35.305753133229175	37.0	35.0	37.0	33.0	37.0
6	35.76610177020198	37.0	35.0	37.0	35.0	37.0
7	35.765766580122055	37.0	35.0	37.0	35.0	37.0
8	35.68678856689966	37.0	35.0	37.0	33.0	37.0
9	37.64630868275182	39.0	37.0	39.0	35.0	39.0
10	37.11796098785823	39.0	37.0	39.0	33.0	39.0
11	37.781894202387576	39.0	37.0	39.0	35.0	39.0
12	37.68089099498549	39.0	37.0	39.0	35.0	39.0
13	37.41987360946593	39.0	37.0	39.0	35.0	39.0
14	37.8787431537051	40.0	38.0	40.0	33.0	40.0
15	36.895674386342954	39.0	36.0	40.0	31.0	40.0
16	37.03778851380402	39.0	36.0	40.0	31.0	40.0
17	36.989993000434424	39.0	36.0	40.0	32.0	40.0
18	36.733976427174426	39.0	36.0	40.0	31.0	40.0
19	36.96770800324773	39.0	36.0	40.0	32.0	40.0
20	37.015730032534584	39.0	36.0	40.0	32.0	40.0
21	37.10589237148851	39.0	36.0	40.0	32.0	40.0
22	37.08656264032759	39.0	36.0	40.0	32.0	40.0
23	37.229675380480984	39.0	37.0	40.0	32.0	40.0
24	37.5011972984198	39.0	37.0	40.0	32.0	40.0
25	37.59571729940422	40.0	37.0	40.0	32.0	40.0
26	37.30076230709654	40.0	37.0	40.0	32.0	40.0
27	36.9287890380484	39.0	36.0	40.0	31.0	40.0
28	36.492778584156014	38.0	36.0	40.0	30.0	40.0
29	36.269333128081136	38.0	35.0	40.0	30.0	40.0
30	36.04642176608966	38.0	35.0	40.0	30.0	40.0
31	35.992593758954435	38.0	35.0	40.0	30.0	40.0
32	35.98174350463834	38.0	35.0	40.0	30.0	40.0
33	35.8903598296203	38.0	35.0	40.0	30.0	40.0
34	35.767600917042955	38.0	35.0	40.0	30.0	40.0
35	35.54187521346711	38.0	35.0	40.0	30.0	40.0
36	35.40253229079624	38.0	35.0	40.0	29.0	40.0
37	35.369458429417975	37.0	35.0	40.0	29.0	40.0
38	35.29413495666648	37.0	35.0	40.0	29.0	40.0
39	35.08076165554269	37.0	35.0	40.0	27.0	40.0
40	34.80744518148367	36.0	34.0	40.0	26.0	40.0
41	34.42089391333559	35.0	33.0	40.0	25.0	40.0
42	34.07043570017456	35.0	33.0	39.0	24.0	40.0
43	33.6906989541251	35.0	33.0	39.0	23.0	40.0
44	33.2502141969656	35.0	33.0	39.0	21.0	40.0
45	32.97294654535396	35.0	33.0	39.0	20.0	40.0
46	32.55276320224641	35.0	33.0	38.0	20.0	40.0
47	32.117534067290016	35.0	32.0	38.0	18.0	40.0
48	31.62281399998243	35.0	31.0	37.0	15.0	40.0
49	31.051712257883487	35.0	30.0	37.0	12.0	40.0
50	30.641196113181124	35.0	28.0	36.0	10.0	40.0
51	30.15168872227397	35.0	27.0	35.0	10.0	40.0
52	29.15564325582007	34.0	24.0	35.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	12.0
11	20.0
12	44.0
13	91.0
14	187.0
15	496.0
16	1457.0
17	3983.0
18	9757.0
19	21143.0
20	40791.0
21	70413.0
22	113369.0
23	170616.0
24	243726.0
25	340893.0
26	458419.0
27	586674.0
28	741884.0
29	935315.0
30	1160771.0
31	1397301.0
32	1666931.0
33	2062612.0
34	2679991.0
35	3576827.0
36	6698076.0
37	7138187.0
38	5912095.0
39	618755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.80354587176514	19.144111980389212	36.97356672203419	9.078775425811452
2	28.297666034775844	20.913487235738025	28.169322476212415	22.619524253273717
3	24.536846980285258	25.582028856850947	26.7811498812333	23.099974281630498
4	18.845701406183093	35.9708722685419	24.8586185432118	20.324807782063203
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	100.0	0.0	0.0
11	97.28757966460037	2.6167936205347075	0.07193558369703117	0.023691131167886275
12	96.96900857349922	2.784857953892797	0.13644432655115246	0.10968914605681954
13	93.6873544474253	5.4257601584421815	0.5140890881481972	0.3727963059843148
14	29.581772819104913	59.507387633016705	5.150642361707012	5.760197186171364
15	18.85988934607403	55.841207594498634	16.094277878333287	9.204625181094046
16	42.092758240832964	38.52192115838199	9.884415764918495	9.500904835866544
17	27.076076224816205	35.08158038152789	14.04304555480111	23.799297838854798
18	21.136251534275637	48.29229589952712	20.792649643066902	9.778802923130343
19	30.121250134307946	43.6805387793777	20.277687450925416	5.920523635388935
20	22.325162698836472	39.42331372105299	31.46782205245685	6.783701527653687
21	26.972946272508963	40.094389635644426	16.36720200128532	16.565462090561294
22	20.71278245528084	50.936352192252265	17.957233511062746	10.393631841404153
23	9.316928182230773	54.487473495154795	22.911621194228367	13.283977128386068
24	10.628982479695011	55.81159026997631	14.1058408310684	19.453586419260283
25	18.72784055243909	44.7158709515191	16.987476905717262	19.568811590324543
26	13.027399149258384	50.75861004681856	6.8763051199418435	29.337685683981213
27	14.935799844838508	62.511361947825165	6.057898363382935	16.494939843953397
28	17.6467413603495	57.544093252308556	7.710831558976839	17.09833382836511
29	21.108882452536022	50.674868460062285	7.03591125836495	21.180337829036745
30	24.829279520587328	51.85179354386526	8.69874599893274	14.620180936614668
31	15.936966366342538	57.43464966674985	10.389863851983117	16.238520114924494
32	17.138330176607656	65.63444674646802	10.129588823133851	7.097634253790486
33	19.865268594345682	63.787217899417655	9.630757227268369	6.716756278968294
34	16.314982197136732	66.1803959450213	10.007550712471957	7.497071145370014
35	17.10115504564342	61.84915895256552	13.260275083418266	7.789410918372798
36	12.953542736776116	64.59784311663638	14.675612126388572	7.773002020198934
37	13.558532864425556	66.61930259399742	11.429116806744378	8.393047734832642
38	12.580814642041277	66.6784963162924	11.555741441821016	9.184947599845309
39	12.27488991659292	68.22347838846612	10.4403019782954	9.061329716645558
40	11.607762069121133	70.73370148127005	9.852408318214536	7.806128131394294
41	11.83440622352892	71.57854146122575	9.552535294819661	7.0345170204256675
42	13.131077520011408	69.33194666229532	10.086473856565275	7.450501961127991
43	14.158006066217407	67.09842932411438	11.015096350026955	7.728468259641265
44	14.778463776797949	67.82580407557305	9.592774475110343	7.802957672518665
45	14.845875590429754	67.15963664249387	10.054469138311296	7.940018628765076
46	15.849287626266525	66.09707453956179	10.332779221521927	7.72085861264976
47	17.964635795855845	64.7188498732062	9.78701555756721	7.529498773370744
48	17.368188445090734	65.27836492505521	9.401728407578913	7.951718222275142
49	15.362496680158488	66.39486029872272	9.835464643844784	8.40717837727402
50	13.950802117910186	67.38613346060279	10.260578978176929	8.402485443310095
51	13.930333286167528	66.22401840328597	10.658018644371811	9.18762966617469
52	15.477247100926403	64.71913363200402	9.997010710202979	9.8066085568666
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5898.0
1	24240.0
2	42582.0
3	122213.0
4	201844.0
5	404850.0
6	607856.0
7	731568.5
8	855281.0
9	1333290.5
10	1811300.0
11	1737130.0
12	1662960.0
13	1086987.0
14	388066.5
15	265119.0
16	293735.0
17	322351.0
18	494548.0
19	666745.0
20	1093967.0
21	1521189.0
22	1941935.0
23	2362681.0
24	2413414.5
25	2464148.0
26	2258436.5
27	2052725.0
28	1890907.5
29	1729090.0
30	1695544.5
31	1661999.0
32	1648674.0
33	1635349.0
34	1592959.0
35	1550569.0
36	1572984.5
37	1595400.0
38	1713729.5
39	1933157.0
40	2034255.0
41	2052891.0
42	2071527.0
43	2016062.5
44	1960598.0
45	1879350.0
46	1798102.0
47	1666701.5
48	1535301.0
49	1295032.0
50	1054763.0
51	791111.5
52	527460.0
53	361353.0
54	195246.0
55	129289.5
56	63333.0
57	43667.5
58	24002.0
59	17589.0
60	11176.0
61	9821.0
62	8466.0
63	6115.0
64	2842.5
65	1921.0
66	1548.5
67	1176.0
68	971.0
69	766.0
70	572.5
71	379.0
72	288.0
73	197.0
74	160.5
75	124.0
76	101.0
77	78.0
78	48.5
79	19.0
80	17.0
81	15.0
82	15.5
83	16.0
84	8.5
85	1.0
86	1.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3.6650846E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.96714818988137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43477434176305	18.833911896610587
2	9.127362899686029	4.557684441985729
3	3.4297817080329076	2.5689560449020616
4	1.9580199755626102	1.9554469955447837
5	1.298496147749447	1.620987287242527
6	0.9681988657974906	1.4503898735784608
7	0.7693258755605256	1.3445511199000915
8	0.6307791602423554	1.2599005415087852
9	0.5455564516700567	1.2258889897312946
>10	5.143650334081203	26.694595873706405
>50	0.4519614272763306	7.598617422446287
>100	0.20528648885782555	9.757196967496467
>500	0.021894467590560764	3.7624393911710485
>1k	0.012874278788249972	5.980456370494122
>5k	0.0011895046107357123	2.034844820177599
>10k+	8.480727302650042E-4	9.35413196350382
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	439806	1.199988671475687	No Hit
CCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	305314	0.8330339768964677	No Hit
AAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	213864	0.5835172263145031	No Hit
GGGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	202726	0.553127750448107	No Hit
GCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	151935	0.41454704756337685	No Hit
GCCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124610	0.33999215188648035	No Hit
TCCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	110314	0.3009862309863188	No Hit
TCGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107901	0.29440248118692813	No Hit
GGAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	106441	0.29041894421754955	No Hit
GAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	104158	0.2841898929154323	No Hit
TAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	87228	0.23799723477051526	No Hit
CCGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76619	0.20905110894302412	No Hit
GTCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49529	0.1351373990111988	No Hit
CCAAAATAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49345	0.13463536421505795	No Hit
ACAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49330	0.13459443746537256	No Hit
TCGCAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44665	0.12186621831321437	No Hit
CGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41911	0.11435206707097567	No Hit
GGCAAATAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40708	0.11106974174620689	No Hit
TAAAAATAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38855	0.10601392393507098	No Hit
CTCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37757	0.10301808585809998	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.2089747123436114	0.0	0.0	0.0	0.0
11	0.21869072271892442	0.0	0.0	0.0	0.0
12	0.33213148749690524	0.0	0.0	0.0	0.0
13	0.5147111747434152	0.0	0.0	0.0	0.0
14	1.1309261456065707	0.0	0.0	0.0	0.0
15	1.8264626142599818	0.0	0.0	0.0	0.0
16	2.1474101852928578	0.0	0.0	0.0	0.0
17	2.394078434096719	0.0	0.0	0.0	0.0
18	2.782500573110918	0.0	0.0	0.0	0.0
19	3.001974360973823	0.0	0.0	0.0	0.0
20	3.1738394251526962	0.0	0.0	0.0	0.0
21	3.3378656525418267	0.0	0.0	0.0	0.0
22	3.4798296334005494	0.0	0.0	0.0	0.0
23	3.6278016611130885	0.0	0.0	0.0	0.0
24	3.801314709079294	0.0	0.0	0.0	0.0
25	3.9623641975413064	0.0	0.0	0.0	0.0
26	4.141967145860699	0.0	0.0	0.0	0.0
27	4.5144633223473205	0.0	0.0	0.0	0.0
28	4.85706387241375	0.0	0.0	0.0	0.0
29	5.155458075919994	0.0	0.0	0.0	0.0
30	5.492784532176965	0.0	0.0	0.0	0.0
31	5.9143764375861885	0.0	0.0	0.0	0.0
32	6.3739865650031655	2.728449979026405E-6	0.0	0.0	0.0
33	6.7174574906129045	2.728449979026405E-6	0.0	0.0	0.0
34	7.013838098034626	2.728449979026405E-6	0.0	0.0	0.0
35	7.299408586639446	2.728449979026405E-6	0.0	0.0	0.0
36	7.6182225097887235	2.728449979026405E-6	0.0	0.0	0.0
37	7.956132854341207	2.728449979026405E-6	0.0	0.0	0.0
38	8.302978872575002	2.728449979026405E-6	0.0	0.0	0.0
39	8.682959187354093	2.728449979026405E-6	0.0	0.0	0.0
40	9.092567740455431	2.728449979026405E-6	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTT	70	0.0	46.000004	37
CGATCTA	25	3.4192366E-5	46.0	44
CGTACTG	180	0.0	44.722218	28
CTAACAT	175	0.0	44.685715	32
GGCAAAT	120540	0.0	43.824787	1
CTACCCG	105	0.0	43.809525	28
ACGTACT	180	0.0	43.444443	27
CCAAAAT	65225	0.0	42.854584	1
CGTCGCT	145	0.0	42.827583	22
CCTATCT	140	0.0	42.714287	31
CGCCCTA	205	0.0	42.634144	21
GCAAATA	177545	0.0	42.59168	2
GCCAAAT	45425	0.0	42.56709	1
CGGTACT	65	0.0	42.46154	27
AGGGAAA	577600	0.0	42.438904	10
CCAAATA	108990	0.0	42.39352	2
CACGACC	60	1.8189894E-12	42.166668	36
CCGAAAT	17990	0.0	42.138966	1
CCCGCCG	115	0.0	42.0	42
CGAACTA	110	0.0	41.81818	24
>>END_MODULE