FastQCFastQC Report
Fri 17 Jun 2016
SRR1528099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35595122
Sequences flagged as poor quality0
Sequence length59
%GC30

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320130.9327485940348793No Hit
CCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2481950.6972725082948164No Hit
AAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1706850.4795179519261094No Hit
GGGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1551610.43590523443071777No Hit
GCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1202280.33776538257124106No Hit
GCCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA972600.273239687168371No Hit
TCCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA831840.23369494280705091No Hit
GGAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA831390.23356852099003902No Hit
GAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830880.2334252429307589No Hit
TCGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA775650.21790907192283257No Hit
TAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA675610.18980409731423312No Hit
CCGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA616690.1732512674068093No Hit
CCAAAATAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA402710.11313628873079856No Hit
ACAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA381000.10703713840340258No Hit
GTCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA378090.10621961065339235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAAAT1122900.050.1444471
TACGTAC1700.049.88235526
CCAAAAT612350.048.836861
GCCAAAT442100.048.72021
CCAAATA1029850.048.5998422
CCTAACT4150.048.53012546
GCAAATA1678100.048.470952
AGGGAAA5370900.048.1982410
CCGAAAT172000.047.7000051
TAAAAGG547850.047.4228367
TAAAGGA1560400.047.1748967
CCCAAAT134300.047.001491
CGAAATA714300.046.9899252
TCCAAAT368350.046.9424481
CAAATAA4140900.046.9370463
TCGAAAT301000.046.529071
ATAAAAG628500.046.3971376
GTCAAAT296050.046.376121
CAAAATA1650900.046.3384862
GGAAATA1056800.046.2095032
Produced by FastQC (version 0.11.5)