FastQCFastQC Report
Fri 17 Jun 2016
SRR1528098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838605
Sequences flagged as poor quality0
Sequence length52
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCGTATTGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGATCACTGACT31460.17110798676170247No Hit
GGTTCGTATTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG28420.15457371213501542No Hit
CCGCCGTATTGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTC28290.15386665433847943No Hit
TTCTCGTATTGGGGAAAAACATGGCCGATCTCCAGCTCAGATCTTGCTTAGA23180.12607384402848898No Hit
CCAGCGTATTGGGAACCAGTGGTATCAACGCAGAATAAGTCGTATTGGGATC20690.11253096777176173No Hit
TATGCGTATTGGGTTCCATTATTACAGGTTTCCATTATTATAAGAAATTACT19570.10643939290929808No Hit
GTTTCGTATTGGGAACACCCAAATCGGAAACAAAAGAAACCTCCCAAACCAG18720.10181632270117834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACT2150.046.00000440
TCCGAGC1450.046.00000418
TAGCGCT1450.046.00000428
TAAGTCA1450.046.00000421
GTTACAT1450.046.00000424
GTTAAGT1450.046.00000419
TTAAGTC1450.046.00000420
CCTCATT1450.046.00000431
GAAACTA2150.046.00000441
GCCCTAC1450.046.00000430
CTCATTT1450.046.00000432
TGTTAAG1450.046.00000418
GCTAGCG1450.046.00000426
TGCTAGC1450.046.00000425
TCCCCGG1450.046.00000424
ATGTTAA1450.046.00000417
CCATTCC1450.046.00000426
CCACAAT1550.046.046
TCCGGAT405.6152203E-946.030
TCACGCC750.046.019
Produced by FastQC (version 0.11.5)