FastQCFastQC Report
Fri 17 Jun 2016
SRR1528097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831661
Sequences flagged as poor quality0
Sequence length52
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCGTATTGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGATCACTGACT31930.17432265031575167No Hit
GGTTCGTATTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG29350.160237074436809No Hit
CCGCCGTATTGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTC26930.14702502264338216No Hit
TTCTCGTATTGGGGAAAAACATGGCCGATCTCCAGCTCAGATCTTGCTTAGA22860.12480475371807337No Hit
CCAGCGTATTGGGAACCAGTGGTATCAACGCAGAATAAGTCGTATTGGGATC20580.11235703549947289No Hit
TATGCGTATTGGGTTCCATTATTACAGGTTTCCATTATTATAAGAAATTACT18890.103130437346212No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG1600.046.00000426
TCTGCAC405.6152203E-946.00000446
CTTGTAC800.046.00000434
CTATGTG1400.046.00000431
CTATGGA1450.046.00000442
CTATGCG206.312439E-446.00000437
GATACTA405.6152203E-946.00000413
ACTATGA206.312439E-446.00000434
CCTTAGC351.0200165E-746.00000419
GATACGC206.312439E-446.00000420
TCTGACA1450.046.00000415
ACAGTCT405.6152203E-946.00000441
TGCCCCT351.0200165E-746.00000443
ACCGGCA700.046.00000432
ATTAGTG1600.046.00000415
CTCAATA206.312439E-446.00000431
ATTAGAG351.0200165E-746.00000435
CAAGTAT351.0200165E-746.00000430
AGATAGT700.046.00000433
CCTAGTC700.046.00000419