##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528097_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1831661
Sequences flagged as poor quality	0
Sequence length	52
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.460939005634778	31.0	31.0	33.0	28.0	33.0
2	31.319197711803657	31.0	31.0	34.0	30.0	34.0
3	31.676661784030998	31.0	31.0	34.0	30.0	34.0
4	35.54460787230825	37.0	35.0	37.0	33.0	37.0
5	35.66581152298378	37.0	35.0	37.0	33.0	37.0
6	35.509193568023775	37.0	35.0	37.0	33.0	37.0
7	33.0623172082607	35.0	32.0	35.0	28.0	35.0
8	34.387773720137076	35.0	35.0	36.0	32.0	37.0
9	37.093420671183154	39.0	37.0	39.0	34.0	39.0
10	37.638395969559866	39.0	37.0	39.0	35.0	39.0
11	37.194751102960645	39.0	37.0	39.0	34.0	39.0
12	37.49756641649301	39.0	37.0	39.0	35.0	39.0
13	37.51564727315808	39.0	37.0	39.0	35.0	39.0
14	37.70639545199685	40.0	38.0	40.0	33.0	40.0
15	36.93170351937395	39.0	36.0	40.0	31.0	40.0
16	36.40272681462345	38.0	35.0	40.0	31.0	40.0
17	36.20463284417804	38.0	35.0	40.0	30.0	40.0
18	36.201242478821136	38.0	35.0	40.0	30.0	40.0
19	36.51808276749901	39.0	35.0	40.0	31.0	40.0
20	36.7262310001687	39.0	35.0	40.0	31.0	40.0
21	36.842495964045746	39.0	35.0	40.0	31.0	40.0
22	36.79014730345845	39.0	35.0	40.0	31.0	40.0
23	36.831962901432085	39.0	35.0	40.0	31.0	40.0
24	36.84777314142737	39.0	35.0	40.0	31.0	40.0
25	36.840295775255356	39.0	35.0	40.0	31.0	40.0
26	36.48003205833394	39.0	35.0	40.0	31.0	40.0
27	36.164392319321095	39.0	35.0	40.0	30.0	40.0
28	35.87171752851647	38.0	35.0	40.0	30.0	40.0
29	35.60961771856255	37.0	35.0	40.0	30.0	40.0
30	35.29088242857166	37.0	34.0	40.0	29.0	40.0
31	35.21001812016525	37.0	34.0	40.0	29.0	40.0
32	35.186865910231205	37.0	34.0	40.0	28.0	40.0
33	35.02116439668694	36.0	34.0	40.0	27.0	40.0
34	34.772867359189284	36.0	33.0	40.0	25.0	40.0
35	34.43035092192278	35.0	33.0	40.0	24.0	40.0
36	34.20145485436443	35.0	33.0	40.0	24.0	40.0
37	33.954323425568376	35.0	33.0	40.0	23.0	40.0
38	33.51578976677453	35.0	33.0	40.0	21.0	40.0
39	33.00733869422344	35.0	33.0	40.0	18.0	40.0
40	32.37527140666313	35.0	32.0	39.0	15.0	40.0
41	31.629314594785825	35.0	31.0	39.0	12.0	40.0
42	30.832063356701923	35.0	28.0	39.0	9.0	40.0
43	30.010635155741156	35.0	24.0	38.0	8.0	40.0
44	29.319193890135782	35.0	22.0	38.0	8.0	40.0
45	28.882400728082324	35.0	20.0	38.0	8.0	40.0
46	28.423733976974997	35.0	19.0	38.0	8.0	40.0
47	27.99489861934059	34.0	17.0	38.0	8.0	40.0
48	27.593708661155095	34.0	15.0	37.0	8.0	40.0
49	27.16040304401306	33.0	12.0	37.0	8.0	40.0
50	26.79039025234473	33.0	10.0	36.0	8.0	40.0
51	26.427472660061003	33.0	10.0	36.0	8.0	40.0
52	25.4524936655855	33.0	10.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	7.0
15	42.0
16	149.0
17	523.0
18	1262.0
19	2728.0
20	5019.0
21	8202.0
22	12517.0
23	18033.0
24	25175.0
25	34279.0
26	45297.0
27	58932.0
28	75814.0
29	94970.0
30	111380.0
31	110007.0
32	95036.0
33	93954.0
34	116424.0
35	150436.0
36	198455.0
37	254945.0
38	278920.0
39	39151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.94678382080527	22.527694808155	36.76258871046553	6.7629326605742
2	27.075697959393143	19.757531551962945	34.20463721179847	18.962133276845442
3	19.88866935530101	21.961869581762127	36.40215083467956	21.7473102282573
4	24.462168490785142	24.618747683113853	39.83215234696814	11.086931479132875
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.36107172670052	0.35852704184890105	0.22023726006067715	0.06016397138990239
12	99.01237183081368	0.679437952765277	0.16323981348076966	0.14495040294028208
13	96.85258352937579	1.798586092077082	0.6724497600811504	0.6763806184659716
14	52.45266454873473	27.914990819807812	10.73413693909517	8.898207692362288
15	31.620916752608696	40.0276033611023	17.86007345245654	10.491406433832463
16	36.193269387730595	34.74250966745484	17.106058380890353	11.958162563924219
17	23.350117734668153	38.615824653142695	17.979145704363418	20.05491190782574
18	17.331427594953432	48.58082363494118	20.03465706809284	14.053091702012546
19	19.51403671312541	45.29806552631737	24.582824005097013	10.605073755460207
20	15.263577703516098	45.99328150787728	24.181166711525766	14.561974077080858
21	21.73322465237836	47.82184039513862	13.798350240573992	16.646584711909025
22	20.404048565755346	48.20089525299714	16.178048230540476	15.217007950707037
23	18.199601345445473	48.10622708022937	20.46437632291128	13.229795251413881
24	18.304806402494787	50.552531281716426	15.444287998707185	15.698374317081601
25	19.28926804687112	48.385973168615806	18.78387976814487	13.540879016368205
26	16.20043228523182	47.473795642315906	19.674219192306875	16.6515528801454
27	18.451667639372133	52.3446205384075	16.023216086382796	13.18049573583758
28	16.364491027542762	50.80001157419414	15.410056773606032	17.425440624657075
29	18.084514547178763	51.58121508292201	14.633220885305743	15.70104948459349
30	21.35569846166949	50.767472802008676	15.322158412501002	12.554670323820838
31	17.590318295798184	53.61991110800525	12.724461567942976	16.06530902825359
32	17.355394912049775	58.61854349685886	12.693724439183887	11.332337151907476
33	19.182315941650774	54.67507360805301	12.611886151422125	13.530724298874084
34	17.581910626475093	56.7118587991992	13.867740810117157	11.838489764208552
35	17.540745803945164	56.40295884445866	15.195824991633277	10.860470359962896
36	16.378522008166357	54.859387190096854	18.335161364466458	10.426929437270324
37	16.58117959600603	55.728543655185106	16.123452975195736	11.56682377361313
38	14.376404804164089	57.54465482422785	18.06677108919172	10.012169282416343
39	16.054499167695333	56.12206625570998	17.577379220281482	10.246055356313203
40	18.493378414455513	54.231104991589596	14.492965674325106	12.782550919629779
41	19.66804992845292	53.26111108987962	14.220699135920894	12.850139845746567
42	22.003088999547405	50.25880880796173	13.636093141689429	14.102009050801431
43	18.80025834474829	50.0417926679664	16.895593671536382	14.262355315748929
44	19.672635929901876	50.04523216905311	16.992664035539327	13.28946786550568
45	20.885742503661977	45.032896371107974	20.242719586211642	13.83864153901841
46	24.927866018875765	44.4966071778566	17.003801467629653	13.57172533563798
47	25.37876823276796	44.05744294386352	15.24861860355164	15.315170219816876
48	25.70175376338744	44.519155018313974	15.81657304490296	13.962518173395623
49	24.565844880684796	44.350947036596835	15.604688858910027	15.478519223808334
50	23.173229107351194	46.88394850357135	14.237732855588453	15.705089533489003
51	21.989385590455875	46.72518550102885	16.73683066899388	14.548598239521397
52	25.616748950815683	43.82688718054269	15.129491756389418	15.426872112252212
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	3.0
4	6.0
5	14.0
6	22.0
7	294.0
8	566.0
9	3286.0
10	6006.0
11	9385.0
12	12764.0
13	17698.5
14	23271.0
15	23909.0
16	24775.0
17	25641.0
18	27456.0
19	29271.0
20	36075.0
21	42879.0
22	52302.0
23	61725.0
24	76523.0
25	91321.0
26	101463.0
27	111605.0
28	113285.0
29	114965.0
30	108206.5
31	101448.0
32	93929.0
33	86410.0
34	82069.5
35	77729.0
36	71552.5
37	65376.0
38	63218.0
39	60705.0
40	60350.0
41	66616.5
42	72883.0
43	79513.0
44	86143.0
45	94609.0
46	103075.0
47	108714.0
48	114353.0
49	109731.0
50	105109.0
51	95333.0
52	85557.0
53	80220.0
54	74883.0
55	65740.5
56	56598.0
57	47050.5
58	37503.0
59	33133.5
60	28764.0
61	27076.0
62	25388.0
63	22736.5
64	15769.5
65	11454.0
66	8905.0
67	6356.0
68	5054.0
69	3752.0
70	2062.5
71	373.0
72	1659.0
73	2945.0
74	1649.5
75	354.0
76	236.5
77	119.0
78	86.0
79	53.0
80	79.0
81	105.0
82	89.0
83	73.0
84	50.5
85	28.0
86	22.5
87	17.0
88	10.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1831661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.44356216097747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.08755966907783	37.38749814423317
2	5.366956429703241	4.555854976787345
3	1.7099352968594113	2.1772723519050565
4	0.8359861634314627	1.4192892277328142
5	0.5106849677018054	1.0837644585664175
6	0.3713896766449219	0.9457860495974438
7	0.3005808191326708	0.893040447687853
8	0.21934395185710118	0.7447790924225058
9	0.1939081796196383	0.7407138487687293
>10	2.0249406861591455	18.505470081064622
>50	0.20514707312871303	5.928317865612236
>100	0.136430679273905	11.94852283187905
>500	0.02712801392365343	7.988490603833685
>1k	0.010008393486396412	5.681200019909093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCGCGTATTGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGATCACTGACT	3193	0.17432265031575167	No Hit
GGTTCGTATTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	2935	0.160237074436809	No Hit
CCGCCGTATTGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTC	2693	0.14702502264338216	No Hit
TTCTCGTATTGGGGAAAAACATGGCCGATCTCCAGCTCAGATCTTGCTTAGA	2286	0.12480475371807337	No Hit
CCAGCGTATTGGGAACCAGTGGTATCAACGCAGAATAAGTCGTATTGGGATC	2058	0.11235703549947289	No Hit
TATGCGTATTGGGTTCCATTATTACAGGTTTCCATTATTATAAGAAATTACT	1889	0.103130437346212	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.011683384643774148	0.0	0.0	0.0	0.0
12	0.06824406918092377	0.0	0.0	0.0	0.0
13	0.08915405197795881	0.0	0.0	0.0	0.0
14	0.4398739723125622	0.0	0.0	0.0	0.0
15	0.8848798986275299	0.0	0.0	0.0	0.0
16	1.1915960431542736	0.0	0.0	0.0	0.0
17	1.3888487007148156	0.0	0.0	0.0	0.0
18	1.583753762295534	0.0	0.0	0.0	0.0
19	1.7878308267741683	0.0	0.0	0.0	0.0
20	1.9946376540200397	0.0	0.0	0.0	0.0
21	2.2095791743122772	0.0	0.0	0.0	0.0
22	2.410052951938159	0.0	0.0	0.0	0.0
23	2.6047396324974983	0.0	0.0	0.0	0.0
24	2.8233390348978333	0.0	0.0	0.0	0.0
25	3.0630122058612375	0.0	0.0	0.0	0.0
26	3.330638147561148	0.0	0.0	0.0	0.0
27	3.623705478251707	0.0	0.0	0.0	0.0
28	3.9164998326655422	0.0	0.0	0.0	0.0
29	4.243580007435874	0.0	0.0	0.0	0.0
30	4.610023361309762	0.0	0.0	0.0	0.0
31	4.920506578455293	0.0	0.0	0.0	0.0
32	5.164001417292829	0.0	0.0	0.0	0.0
33	5.368406053303532	0.0	0.0	0.0	0.0
34	5.56746035429045	0.0	0.0	0.0	0.0
35	5.750136078673947	0.0	0.0	0.0	0.0
36	5.919654346519361	0.0	0.0	0.0	0.0
37	6.076015157826694	0.0	0.0	0.0	0.0
38	6.232867326432129	0.0	0.0	0.0	0.0
39	6.391248162187217	0.0	0.0	0.0	0.0
40	6.542859186279557	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	160	0.0	46.000004	26
TCTGCAC	40	5.6152203E-9	46.000004	46
CTTGTAC	80	0.0	46.000004	34
CTATGTG	140	0.0	46.000004	31
CTATGGA	145	0.0	46.000004	42
CTATGCG	20	6.312439E-4	46.000004	37
GATACTA	40	5.6152203E-9	46.000004	13
ACTATGA	20	6.312439E-4	46.000004	34
CCTTAGC	35	1.0200165E-7	46.000004	19
GATACGC	20	6.312439E-4	46.000004	20
TCTGACA	145	0.0	46.000004	15
ACAGTCT	40	5.6152203E-9	46.000004	41
TGCCCCT	35	1.0200165E-7	46.000004	43
ACCGGCA	70	0.0	46.000004	32
ATTAGTG	160	0.0	46.000004	15
CTCAATA	20	6.312439E-4	46.000004	31
ATTAGAG	35	1.0200165E-7	46.000004	35
CAAGTAT	35	1.0200165E-7	46.000004	30
AGATAGT	70	0.0	46.000004	33
CCTAGTC	70	0.0	46.000004	19
>>END_MODULE