FastQCFastQC Report
Fri 17 Jun 2016
SRR1528096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666892
Sequences flagged as poor quality0
Sequence length59
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCGTATTGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGATCACTGACTGCTCTTC24790.14871989307045685No Hit
CCGCCGTATTGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGC22310.1338419045745015No Hit
TATGCGTATTGGGTTCCATTATTACAGGTTTCCATTATTATAAGAAATTACTAAATAGA18300.10978515704676728No Hit
TTCTCGTATTGGGGAAAAACATGGCCGATCTCCAGCTCAGATCTTGCTTAGATGGCAGG18120.10870530304302858No Hit
CCAGCGTATTGGGAACCAGTGGTATCAACGCAGAATAAGTCGTATTGGGATCCCTGCTT17760.10654559503555121No Hit
GTCACGTATTGGGGACAAAATGAATACTTTCCATCCCATTTTTCAAAATTAGTGAATGT17520.10510578969723293No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATAT251.4790219E-553.00000416
GATACTA503.6379788E-1253.00000413
ATCTCCG750.053.00000435
TCCGAGC1000.053.00000418
TTAGAAT251.4790219E-553.0000049
TTCACAG1350.053.00000416
ATTGTCC251.4790219E-553.00000430
ATTAAGG503.6379788E-1253.00000426
TTCAATT503.6379788E-1253.00000451
CTAAGTG503.6379788E-1253.00000422
CAATTAG251.4790219E-553.00000439
CTAAGCA503.6379788E-1253.00000443
TCGATCA1000.053.00000427
CATTAAG503.6379788E-1253.00000425
GACCAAG1000.053.00000452
GTTACAT1350.053.00000424
ATATCCG251.4790219E-553.00000429
CTTATGT1000.053.00000439
CTCATTT1000.053.00000432
ACTCATT503.6379788E-1253.00000416