FastQCFastQC Report
Fri 17 Jun 2016
SRR1528095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8763479
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT750.046.045
CGTTAAC900.046.025
TACGATA551.8189894E-1246.046
TACTAAC551.8189894E-1246.033
CGGTAAT453.110472E-1046.035
CGATAAC650.046.021
TTATACG950.045.99999636
ACTACGT700.045.99999630
CGTACCA950.045.99999633
TATACGA950.045.99999637
GCGTATT3550.045.3521126
CAATTGA3400.045.3235315
CGCACTA2150.044.93023335
ATATGCG2100.044.90476216
CCGATAG2050.044.87804825
TAACGCC16700.044.760481
TACTCGA1600.044.562516
TAAACTA1550.044.51613221
CGCGATT1450.044.41379517
GTACCGA1350.044.296314
Produced by FastQC (version 0.11.5)