##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8763479
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29454135737645	31.0	31.0	34.0	30.0	34.0
2	31.82702714298739	31.0	31.0	34.0	30.0	34.0
3	31.98174765980497	31.0	31.0	34.0	30.0	34.0
4	35.53142695954426	37.0	35.0	37.0	33.0	37.0
5	35.46336198215344	37.0	35.0	37.0	33.0	37.0
6	35.45724340755538	37.0	35.0	37.0	32.0	37.0
7	35.835686603459656	37.0	35.0	37.0	35.0	37.0
8	35.656648347077684	37.0	35.0	37.0	33.0	37.0
9	37.32681472734744	39.0	37.0	39.0	34.0	39.0
10	37.23239640330056	39.0	37.0	39.0	33.0	39.0
11	37.41019770801071	39.0	37.0	39.0	35.0	39.0
12	37.370216554407214	39.0	37.0	39.0	35.0	39.0
13	37.26593605119611	39.0	37.0	39.0	35.0	39.0
14	37.68581370480833	40.0	38.0	40.0	33.0	40.0
15	35.90121400416433	38.0	35.0	40.0	31.0	40.0
16	35.80119276830583	38.0	35.0	40.0	29.0	40.0
17	35.73407273526872	38.0	34.0	40.0	27.0	40.0
18	36.163016651263725	38.0	35.0	40.0	30.0	40.0
19	36.354194721069106	38.0	35.0	40.0	31.0	40.0
20	36.455852749804045	38.0	35.0	40.0	31.0	40.0
21	36.37695794101863	38.0	35.0	40.0	31.0	40.0
22	36.507388104655696	38.0	35.0	40.0	31.0	40.0
23	36.47157709854728	38.0	35.0	40.0	31.0	40.0
24	36.42032040015158	38.0	35.0	40.0	31.0	40.0
25	36.4956554354726	39.0	35.0	40.0	31.0	40.0
26	36.31309768643252	38.0	35.0	40.0	31.0	40.0
27	36.091766637427895	38.0	35.0	40.0	30.0	40.0
28	35.98193194734648	38.0	35.0	40.0	30.0	40.0
29	36.010241138251146	38.0	35.0	40.0	30.0	40.0
30	35.950444908922584	38.0	35.0	40.0	30.0	40.0
31	35.932197019014936	38.0	35.0	40.0	30.0	40.0
32	35.89706508111676	38.0	35.0	40.0	30.0	40.0
33	35.778588161162936	38.0	35.0	40.0	30.0	40.0
34	35.635162930155936	38.0	35.0	40.0	30.0	40.0
35	35.56236569974094	38.0	34.0	40.0	30.0	40.0
36	35.42491229795838	37.0	34.0	40.0	29.0	40.0
37	35.256195285000395	37.0	34.0	40.0	28.0	40.0
38	34.98640539904301	37.0	34.0	40.0	27.0	40.0
39	34.71400365083319	36.0	34.0	40.0	26.0	40.0
40	34.479024369203145	36.0	33.0	40.0	26.0	40.0
41	34.275970422248975	35.0	33.0	40.0	25.0	40.0
42	34.080745900115694	35.0	33.0	40.0	24.0	40.0
43	33.847581879297024	35.0	33.0	39.0	23.0	40.0
44	33.60628170615802	35.0	33.0	39.0	23.0	40.0
45	33.45644806132359	35.0	33.0	39.0	23.0	40.0
46	33.31451607289753	35.0	33.0	39.0	23.0	40.0
47	32.998285840589105	35.0	33.0	38.0	22.0	40.0
48	32.81078553391866	35.0	33.0	38.0	22.0	40.0
49	32.71316893667458	35.0	32.0	38.0	22.0	40.0
50	32.55668028644788	35.0	32.0	37.0	20.0	40.0
51	32.37179469477818	35.0	32.0	37.0	20.0	40.0
52	31.56385814355235	35.0	31.0	36.0	18.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	13.0
14	43.0
15	143.0
16	505.0
17	1347.0
18	3007.0
19	6172.0
20	11795.0
21	19676.0
22	30383.0
23	44786.0
24	63666.0
25	87235.0
26	115474.0
27	149748.0
28	190451.0
29	237222.0
30	280542.0
31	319302.0
32	373924.0
33	484556.0
34	791711.0
35	1076998.0
36	863290.0
37	1398484.0
38	1825128.0
39	387871.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.97368248386286	21.343658152201883	34.627081322383496	17.055578041551765
2	25.703707397484493	21.228019146277408	34.315150410014105	18.753123046223994
3	27.574859253956106	20.688039533158005	34.23804632840451	17.49905488448138
4	27.713023560620158	20.441345269384453	38.242814297837654	13.602816872157735
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	98.85874091784781	0.9700485389421256	0.12869318223961054	0.04251736097045477
12	98.92840503183724	0.6577068308145657	0.27064593867344233	0.14324219867475008
13	95.37734956630808	2.4551664926680377	1.2115051567990291	0.9559787842248495
14	56.72187951839675	26.577059179351032	7.443881590861347	9.257179711390876
15	33.149894008988895	33.029142878073884	15.50793925563124	18.313023857305986
16	30.76261151535823	22.104680116195862	25.346600362709832	21.786108005736075
17	21.56199609766852	22.39077653977376	28.85001493128471	27.197212431273016
18	20.002934907472252	24.847654681434165	29.958353297817	25.19105711327659
19	26.06867660663077	23.342099638739363	26.19982315242611	24.389400602203757
20	26.447510172615235	23.16566286060593	25.73886466778776	24.647962298991075
21	29.154825383845846	23.556147050731795	22.394496523583843	24.894531041838523
22	30.371237267756335	24.070098188173898	20.875020069084435	24.683644474985332
23	26.50230576235762	23.745889047032577	22.04538859510019	27.706416595509616
24	26.709517989373854	26.30891224820645	20.48841561667461	26.493154145745084
25	27.898223981594523	22.932559089831788	24.09990370262769	25.069313225946
26	28.706898253536068	25.04932116571512	20.11156756352129	26.132213017227517
27	30.209611958903537	26.435528629668653	20.02759406395565	23.327265347472164
28	28.816067226269386	26.645114343287634	19.466002029559267	25.072816400883713
29	29.242187948416376	25.486613250285643	18.70129431473505	26.56990448656293
30	29.37201081899095	24.761547326124706	20.533774314972398	25.332667539911945
31	27.25182544512288	25.73464260027325	20.582841586086985	26.430690368516885
32	27.534361638796646	28.24608811181039	19.255971287202264	24.9635789621907
33	27.975989900814508	27.31431204433764	19.913484131131025	24.796213923716827
34	26.239225312230452	28.742740183436283	20.244984896979844	24.773049607353425
35	29.280357720946213	28.157732790824287	17.931212022074796	24.630697466154707
36	28.469424072334743	27.83429959722617	20.48051920932315	23.21575712111594
37	29.16447908416281	28.25889124627331	19.70300836003601	22.873621309527874
38	27.629426623832842	28.725612282519304	21.568751405691735	22.07620968795612
39	28.835614257762245	28.578695744007604	21.71804143080619	20.867648567423966
40	29.443397992966037	27.669034181516267	20.562176277252448	22.32539154826525
41	28.951424428585952	28.89497424481761	18.45203257747294	23.701568749123496
42	29.672199819272688	26.50389189042388	19.71214856565526	24.11175972464817
43	28.804176971269058	26.74354557134216	18.678951589887987	25.773325867500795
44	28.41825717845618	27.254415740598	21.285975581159036	23.041351499786785
45	26.765682898310132	28.790803287142015	20.02309813260236	24.420415681945492
46	28.220949693609125	26.893953873798292	19.93537041624679	24.949726016345792
47	28.863160395546107	25.387485951640894	21.943454192107954	23.805899460705046
48	27.586954906835516	24.87158353434749	22.655089377175436	24.886372181641562
49	28.380635133603903	26.2280653608002	21.423021610481406	23.968277895114486
50	28.330095844355878	27.099602794734828	20.88397769881117	23.686323662098125
51	26.45369493097433	28.202509528464663	21.522103265153028	23.821692275407973
52	28.50836979240779	26.65970900369591	20.640375814217162	24.191545389679145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	56.0
10	108.0
11	1386.5
12	2665.0
13	5102.0
14	13218.0
15	18897.0
16	21082.0
17	23267.0
18	24711.0
19	26155.0
20	24649.0
21	23143.0
22	25564.0
23	27985.0
24	33038.0
25	38091.0
26	45801.5
27	53512.0
28	63852.5
29	74193.0
30	85087.0
31	95981.0
32	112499.0
33	129017.0
34	148372.0
35	167727.0
36	172818.0
37	177909.0
38	171042.5
39	158680.5
40	153185.0
41	173625.5
42	194066.0
43	205478.0
44	216890.0
45	210306.5
46	203723.0
47	217147.5
48	230572.0
49	279313.0
50	328054.0
51	346926.5
52	365799.0
53	406160.0
54	446521.0
55	490517.5
56	534514.0
57	577955.0
58	621396.0
59	651840.5
60	682285.0
61	700793.0
62	719301.0
63	734463.0
64	703801.5
65	657978.0
66	583522.0
67	509066.0
68	437405.5
69	365745.0
70	317203.0
71	268661.0
72	250390.5
73	232120.0
74	187061.5
75	142003.0
76	104960.5
77	67918.0
78	44790.0
79	21662.0
80	15817.0
81	9972.0
82	10370.5
83	10769.0
84	5968.0
85	1167.0
86	620.5
87	74.0
88	50.0
89	21.0
90	16.0
91	9.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	8763479.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.735003727260457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.65881501009872	9.70001502234441
2	6.931809080138093	1.6268961078415682
3	2.556186319524549	0.899905679615783
4	1.4805938381137873	0.6949909683529665
5	0.9705412659254367	0.5694652686547541
6	0.7276458638624967	0.5123356154731233
7	0.5194765528825831	0.42672414990110685
8	0.384625525448271	0.36108655797879774
9	0.30437112551111983	0.32146166631091067
>10	2.2497508454740287	5.345185051242894
>50	0.26200478205706323	2.1372830483797616
>100	0.4155981581274814	13.304987535636123
>500	0.329155616175866	28.26372069254007
>1k	0.20932523416940366	35.7649698343242
>5k	1.007824911744842E-4	0.07097280140360572
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.14012699750863783	0.0	0.0	0.0	0.0
10	0.14012699750863783	0.0	0.0	0.0	0.0
11	0.14380133734559072	0.0	0.0	0.0	0.0
12	0.15913771231722013	0.0	0.0	0.0	0.0
13	0.19815189834995897	0.0	0.0	0.0	0.0
14	0.37883356598446805	0.0	0.0	0.0	0.0
15	0.5066937457144588	0.0	0.0	0.0	0.0
16	0.5957907812639249	0.0	0.0	0.0	0.0
17	0.6747320327919997	0.0	0.0	0.0	0.0
18	0.7811509561442437	0.0	0.0	0.0	0.0
19	0.8365056845574685	0.0	0.0	0.0	0.0
20	0.8844889113102228	0.0	0.0	0.0	0.0
21	0.9335675934180935	0.0	0.0	0.0	0.0
22	0.9880208533620038	0.0	0.0	0.0	0.0
23	1.0389252943950684	0.0	0.0	0.0	1.1410993282462364E-5
24	1.0976690878131847	0.0	0.0	0.0	2.282198656492473E-5
25	1.1607490586786366	0.0	0.0	0.0	2.282198656492473E-5
26	1.2451561759890108	0.0	0.0	0.0	2.282198656492473E-5
27	1.309548411081946	0.0	0.0	0.0	2.282198656492473E-5
28	1.375127389476257	0.0	0.0	0.0	3.423297984738709E-5
29	1.4377166876305632	0.0	0.0	0.0	3.423297984738709E-5
30	1.5136112039522203	0.0	0.0	0.0	3.423297984738709E-5
31	1.587714194328531	0.0	0.0	0.0	3.423297984738709E-5
32	1.6749512379729556	0.0	0.0	2.282198656492473E-5	3.423297984738709E-5
33	1.7268484354215945	0.0	0.0	2.282198656492473E-5	3.423297984738709E-5
34	1.786459464329178	0.0	0.0	2.282198656492473E-5	3.423297984738709E-5
35	1.832240369378417	0.0	0.0	2.282198656492473E-5	3.423297984738709E-5
36	1.8836012501427801	0.0	0.0	2.282198656492473E-5	3.423297984738709E-5
37	1.933524345753553	0.0	0.0	2.282198656492473E-5	3.423297984738709E-5
38	1.981576038466002	0.0	0.0	2.282198656492473E-5	3.423297984738709E-5
39	2.0337356887601374	0.0	0.0	2.282198656492473E-5	3.423297984738709E-5
40	2.0910759300045108	0.0	0.0	2.282198656492473E-5	3.423297984738709E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	75	0.0	46.0	45
CGTTAAC	90	0.0	46.0	25
TACGATA	55	1.8189894E-12	46.0	46
TACTAAC	55	1.8189894E-12	46.0	33
CGGTAAT	45	3.110472E-10	46.0	35
CGATAAC	65	0.0	46.0	21
TTATACG	95	0.0	45.999996	36
ACTACGT	70	0.0	45.999996	30
CGTACCA	95	0.0	45.999996	33
TATACGA	95	0.0	45.999996	37
GCGTATT	355	0.0	45.35211	26
CAATTGA	340	0.0	45.32353	15
CGCACTA	215	0.0	44.930233	35
ATATGCG	210	0.0	44.904762	16
CCGATAG	205	0.0	44.878048	25
TAACGCC	1670	0.0	44.76048	1
TACTCGA	160	0.0	44.5625	16
TAAACTA	155	0.0	44.516132	21
CGCGATT	145	0.0	44.413795	17
GTACCGA	135	0.0	44.2963	14
>>END_MODULE