FastQCFastQC Report
Fri 17 Jun 2016
SRR1528094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8704197
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG206.313751E-446.00000438
CGAATTA206.313751E-446.00000424
CGTAACG800.046.00000435
TCGAATT206.313751E-446.00000423
GATACGT501.6370905E-1146.032
ACCGGTA2300.046.031
ATATGCG1550.046.016
TAGATTC650.046.031
CACGTCA1350.046.016
TGTAACG750.046.037
TAATCGT1350.046.043
CGATTAC453.110472E-1045.99999639
ACTACGT950.045.99999630
CAATACG850.045.99999638
TACGTCG700.045.99999623
CCGATAG2300.045.025
ACGCGTT2100.044.90476246
TACTCGA1850.044.75675616
TAACGCC18250.044.7397271
ATCGTTT3450.044.66666833
Produced by FastQC (version 0.11.5)