##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528094_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8704197
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32273993798624	31.0	31.0	34.0	30.0	34.0
2	31.846280937805062	31.0	31.0	34.0	30.0	34.0
3	32.001621286834386	33.0	31.0	34.0	30.0	34.0
4	35.56894495839191	37.0	35.0	37.0	33.0	37.0
5	35.658705909344654	37.0	35.0	37.0	33.0	37.0
6	35.529786952202485	37.0	35.0	37.0	33.0	37.0
7	35.849212282304734	37.0	35.0	37.0	35.0	37.0
8	35.66627053592652	37.0	35.0	37.0	33.0	37.0
9	37.30842534928839	39.0	37.0	39.0	34.0	39.0
10	37.18973467627169	39.0	37.0	39.0	33.0	39.0
11	37.40072748813015	39.0	37.0	39.0	35.0	39.0
12	37.389014747713084	39.0	37.0	39.0	35.0	39.0
13	37.26161563209105	39.0	37.0	39.0	35.0	39.0
14	37.62934742860255	40.0	38.0	40.0	32.0	40.0
15	35.89525547273344	38.0	35.0	40.0	31.0	40.0
16	35.762320981475945	38.0	35.0	40.0	27.0	40.0
17	35.71556342302455	38.0	34.0	40.0	27.0	40.0
18	36.06693667434227	38.0	35.0	40.0	30.0	40.0
19	36.3284289176819	38.0	35.0	40.0	30.0	40.0
20	36.43629929331792	38.0	35.0	40.0	31.0	40.0
21	36.40130904665875	38.0	35.0	40.0	31.0	40.0
22	36.463080856281174	38.0	35.0	40.0	31.0	40.0
23	36.495283711984	38.0	35.0	40.0	31.0	40.0
24	36.53161790800461	39.0	35.0	40.0	31.0	40.0
25	36.52973157661758	39.0	35.0	40.0	31.0	40.0
26	36.21679173851419	38.0	35.0	40.0	30.0	40.0
27	35.97162288491403	38.0	35.0	40.0	30.0	40.0
28	35.89305101895097	38.0	35.0	40.0	30.0	40.0
29	35.9169699399037	38.0	35.0	40.0	30.0	40.0
30	35.74182856844807	38.0	35.0	40.0	30.0	40.0
31	35.77148012619659	38.0	35.0	40.0	30.0	40.0
32	35.819420217626046	38.0	35.0	40.0	30.0	40.0
33	35.809573818239635	38.0	35.0	40.0	30.0	40.0
34	35.70824545905843	38.0	35.0	40.0	30.0	40.0
35	35.46057585783042	38.0	34.0	40.0	29.0	40.0
36	35.36850521650647	37.0	34.0	40.0	29.0	40.0
37	35.206304154191365	37.0	34.0	40.0	28.0	40.0
38	34.9951516492561	37.0	34.0	40.0	27.0	40.0
39	34.72676365206348	36.0	34.0	40.0	27.0	40.0
40	34.43599254474594	36.0	33.0	40.0	26.0	40.0
41	34.204720550327615	35.0	33.0	40.0	25.0	40.0
42	34.00585384269221	35.0	33.0	39.0	24.0	40.0
43	33.80643992777277	35.0	33.0	39.0	23.0	40.0
44	33.483975374178684	35.0	33.0	39.0	23.0	40.0
45	33.327119319565035	35.0	33.0	39.0	23.0	40.0
46	33.17739373316114	35.0	33.0	38.0	23.0	40.0
47	32.991794762917245	35.0	33.0	38.0	23.0	40.0
48	32.806559869911034	35.0	33.0	38.0	22.0	40.0
49	32.52727448608987	35.0	32.0	38.0	20.0	40.0
50	32.3650577991284	35.0	32.0	37.0	20.0	40.0
51	32.18766624882226	35.0	32.0	37.0	20.0	40.0
52	31.04093519482613	34.0	30.0	36.0	17.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	9.0
13	23.0
14	62.0
15	125.0
16	477.0
17	1251.0
18	2988.0
19	6088.0
20	11612.0
21	19575.0
22	30662.0
23	45828.0
24	64522.0
25	87925.0
26	116420.0
27	150955.0
28	193330.0
29	239607.0
30	283883.0
31	321641.0
32	376527.0
33	488230.0
34	798774.0
35	1058745.0
36	865634.0
37	1400839.0
38	1804134.0
39	334324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.017483634619023	21.27614988493482	34.62984580886669	17.07652067157947
2	25.678256133219413	21.20346081321459	34.34612061284918	18.772162440716816
3	27.56204851521628	20.652209503070758	34.26063311756386	17.5251088641491
4	27.716709536790123	20.43413079919951	38.23350965057431	13.615650013436047
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	98.86073350591674	0.9678204663796097	0.1283978292311169	0.043048198472529976
12	98.9639136154662	0.6364056328228784	0.26384972674676366	0.13583102496416385
13	95.38362929975045	2.4593538036880367	1.2053610459414004	0.9516558506201089
14	56.711549612215805	26.60383261086577	7.432460455571031	9.252157321347392
15	33.179683318288866	33.09975635891513	15.34702167241849	18.37353865037751
16	30.80742542936471	22.09819010300433	25.317315313520595	21.777069154110368
17	21.538839251914908	22.402434136084008	28.805689944747343	27.25303666725374
18	20.017033162277922	24.864843936781302	29.91979616270174	25.198326738239036
19	26.110622266476735	23.36734795869165	26.153348781053552	24.368680993778057
20	26.44830993599984	23.181070005653595	25.722970194723306	24.64764986362326
21	29.154579107067548	23.595203555250414	22.364486925100614	24.885730412581424
22	30.364076088810947	24.079785878008046	20.90272083685606	24.653417196324945
23	26.46178619348804	23.769533249304907	22.106978966583593	27.661701590623466
24	26.74584456211182	26.338408930772133	20.418827836732095	26.496918670383955
25	27.86492539173918	22.93542988514621	24.120984394080235	25.078660329034374
26	28.690573064924884	25.045848571671804	20.083345999636727	26.18023236376658
27	30.202085269899108	26.508200584154977	19.96344981622084	23.326264329725074
28	28.8095156853642	26.656634724604693	19.44706674263002	25.086782847401086
29	29.213412793851056	25.484590939290552	18.703735680614766	26.598260586243626
30	29.357825885604385	24.74661361639678	20.584816726919207	25.31074377107963
31	27.207851568616842	25.748199403115528	20.57912981519145	26.464819213076176
32	27.523147741256317	28.28548113053967	19.25469977299457	24.936671355209448
33	27.98864731577192	27.358778759258325	19.86538218287109	24.78719174209867
34	26.28268868455068	28.779196978193394	20.142857520343345	24.795256816912577
35	29.129269477701392	28.141205903313082	18.13895066942993	24.5905739495556
36	28.448976970535018	27.844372088545335	20.481889369002104	23.224761571917547
37	29.135151697508682	28.291259951951915	19.710732650007806	22.862855700531597
38	27.648087468608534	28.748142993546676	21.540711911736373	22.063057626108417
39	28.83375686464817	28.629901184451594	21.664973805165484	20.871368145734753
40	29.435880185156655	27.69297386077084	20.5360586392978	22.3350873147747
41	28.95454916748782	28.901528768248237	18.46688442368664	23.677037640577296
42	29.681302020163375	26.49690718167339	19.67795535877692	24.14383543938631
43	28.81107815000051	26.727933662347027	18.610883921859763	25.850104265792694
44	28.380446812037917	27.267983479693765	21.336063510511078	23.015506197757244
45	26.78594016197014	28.79518926329448	20.03489810720047	24.383972467534914
46	28.237331944578	26.870462605568324	19.920907121013002	24.971298328840675
47	28.91885374377441	25.399149398847477	21.882868689667752	23.79912816771036
48	27.604315481370655	24.834582673163304	22.62403987409752	24.937061971368525
49	28.295820970044684	26.22184447341897	21.511978646623003	23.970355909913344
50	28.297176637890892	27.14473259279403	20.86245290633932	23.695637862975758
51	26.399333562877768	28.24047985127175	21.535725811352847	23.824460774497634
52	28.477342596910432	26.647570131971964	20.673210865976493	24.201876405141107
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	62.5
10	122.0
11	1313.0
12	2504.0
13	5066.0
14	13234.0
15	18840.0
16	20965.5
17	23091.0
18	24500.0
19	25909.0
20	24404.5
21	22900.0
22	25259.0
23	27618.0
24	32528.5
25	37439.0
26	45308.0
27	53177.0
28	63891.5
29	74606.0
30	85495.0
31	96384.0
32	113008.0
33	129632.0
34	148517.0
35	167402.0
36	172163.0
37	176924.0
38	169828.0
39	157857.0
40	152982.0
41	172696.5
42	192411.0
43	203175.0
44	213939.0
45	207390.0
46	200841.0
47	215066.5
48	229292.0
49	276221.5
50	323151.0
51	343501.5
52	363852.0
53	403918.5
54	443985.0
55	487225.0
56	530465.0
57	572973.5
58	615482.0
59	645669.0
60	675856.0
61	695215.0
62	714574.0
63	730572.5
64	700109.5
65	653648.0
66	578565.5
67	503483.0
68	434123.0
69	364763.0
70	316918.0
71	269073.0
72	250380.0
73	231687.0
74	186139.5
75	140592.0
76	104070.5
77	67549.0
78	44513.5
79	21478.0
80	15522.0
81	9566.0
82	10199.0
83	10832.0
84	5964.5
85	1097.0
86	583.5
87	70.0
88	50.5
89	22.0
90	13.0
91	7.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	8704197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.876406518481533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.5293631003122	9.801522658906771
2	7.0203018702426405	1.6675191778691558
3	2.6350183675348555	0.9388364794952857
4	1.4937414786695362	0.709611241367885
5	0.941524264688246	0.5590962457226009
6	0.6996287934091283	0.49854455775369244
7	0.5224505123331107	0.434338426917988
8	0.39346686538955356	0.3738377955935191
9	0.300469847816075	0.3211651853288988
>10	2.2509434307575753	5.431534274902069
>50	0.26022554353918775	2.129408579070731
>100	0.41921459109047654	13.493275169785809
>500	0.33137053458573046	28.713599147656403
>1k	0.2021802624550675	34.858051910580194
>5k	1.0053717675537918E-4	0.06965914904896917
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.14239107869456538	0.0	0.0	0.0	0.0
10	0.14239107869456538	0.0	0.0	0.0	0.0
11	0.14565387249392447	0.0	0.0	0.0	0.0
12	0.1610257672247078	0.0	0.0	0.0	0.0
13	0.20168431390052408	0.0	0.0	0.0	0.0
14	0.3821145132629696	0.0	0.0	0.0	0.0
15	0.5128560394485557	0.0	0.0	0.0	0.0
16	0.6026518011942974	0.0	0.0	0.0	0.0
17	0.6800282668234646	0.0	0.0	0.0	0.0
18	0.7858967346442182	0.0	0.0	0.0	0.0
19	0.8405715082046052	0.0	0.0	0.0	0.0
20	0.888157747348779	0.0	0.0	0.0	0.0
21	0.9364447978371813	0.0	0.0	0.0	0.0
22	0.9919008037157248	0.0	0.0	0.0	0.0
23	1.041842228524929	0.0	0.0	0.0	0.0
24	1.099975103964214	0.0	0.0	0.0	0.0
25	1.1622324264949426	0.0	0.0	0.0	1.1488710561123559E-5
26	1.245020074798399	0.0	0.0	0.0	1.1488710561123559E-5
27	1.3097934249420136	0.0	0.0	0.0	1.1488710561123559E-5
28	1.375187165455929	0.0	0.0	0.0	1.1488710561123559E-5
29	1.4383176299893028	0.0	0.0	0.0	1.1488710561123559E-5
30	1.5129253163732392	0.0	0.0	0.0	1.1488710561123559E-5
31	1.585694809067396	0.0	0.0	0.0	1.1488710561123559E-5
32	1.6764326450791498	0.0	0.0	1.1488710561123559E-5	1.1488710561123559E-5
33	1.7292002926863903	0.0	0.0	1.1488710561123559E-5	1.1488710561123559E-5
34	1.788861166630305	0.0	0.0	1.1488710561123559E-5	1.1488710561123559E-5
35	1.8349194072698491	0.0	0.0	1.1488710561123559E-5	1.1488710561123559E-5
36	1.8857914176345043	0.0	0.0	1.1488710561123559E-5	1.1488710561123559E-5
37	1.9345035504136683	0.0	0.0	1.1488710561123559E-5	1.1488710561123559E-5
38	1.980780076553874	0.0	0.0	1.1488710561123559E-5	1.1488710561123559E-5
39	2.03360516771392	0.0	0.0	1.1488710561123559E-5	1.1488710561123559E-5
40	2.089784962357814	0.0	0.0	1.1488710561123559E-5	1.1488710561123559E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	6.313751E-4	46.000004	38
CGAATTA	20	6.313751E-4	46.000004	24
CGTAACG	80	0.0	46.000004	35
TCGAATT	20	6.313751E-4	46.000004	23
GATACGT	50	1.6370905E-11	46.0	32
ACCGGTA	230	0.0	46.0	31
ATATGCG	155	0.0	46.0	16
TAGATTC	65	0.0	46.0	31
CACGTCA	135	0.0	46.0	16
TGTAACG	75	0.0	46.0	37
TAATCGT	135	0.0	46.0	43
CGATTAC	45	3.110472E-10	45.999996	39
ACTACGT	95	0.0	45.999996	30
CAATACG	85	0.0	45.999996	38
TACGTCG	70	0.0	45.999996	23
CCGATAG	230	0.0	45.0	25
ACGCGTT	210	0.0	44.904762	46
TACTCGA	185	0.0	44.756756	16
TAACGCC	1825	0.0	44.739727	1
ATCGTTT	345	0.0	44.666668	33
>>END_MODULE