FastQCFastQC Report
Fri 17 Jun 2016
SRR1528093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7802678
Sequences flagged as poor quality0
Sequence length59
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG4401350.050.4983258
ATTGGCC68700.050.4155731
TAACGCC16700.050.3023951
AGGGGGG1596300.050.0998279
AGGGGGC911750.049.8231969
ACGCCTA286850.049.581843
TTGGCCT296450.049.3796582
AGCCTAG1718200.049.3077054
AGGGGGT585400.049.3061189
TAGCCTA580750.049.299353
TTGCCTA1077750.049.2576643
CGAACTA2100.049.21428719
CAATACG700.049.21428738
TAGGGGC727350.049.0323758
TGGCCTA863050.049.0267683
AGGGGGA1387900.048.9922569
AGGGGAT328300.048.931779
TTCGATA650.048.92307737
GAGCCTA439000.048.919363
CGCCTAG1169300.048.8730474
Produced by FastQC (version 0.11.5)