FastQCFastQC Report
Fri 17 Jun 2016
SRR1528092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10581487
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA1150.046.00000426
TTTACGC351.02063495E-746.00000433
ACGTACT351.02063495E-746.00000417
TAGCGTA650.046.027
TAACCTA1200.046.023
ATATACG453.110472E-1046.035
TACGAAT206.313816E-446.028
CGCTAAT501.6370905E-1146.044
CTCATAA2000.046.040
AACGACT750.046.043
TTACGTG206.313816E-446.024
TATAGCG750.046.016
ACGTAAT405.6188583E-946.044
CGATATA800.046.024
TCGAATA800.046.023
CGTACTA551.8189894E-1245.99999626
ATAAGTC1500.044.46666317
ATGGGAT572750.044.3535589
TATACGT1350.044.29629525
CGCTATA1300.044.2307722
Produced by FastQC (version 0.11.5)